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The map label for this gene is phaA [H]

Identifier: 89052984

GI number: 89052984

Start: 491500

End: 492675

Strand: Reverse

Name: phaA [H]

Synonym: Jann_0493

Alternate gene names: 89052984

Gene position: 492675-491500 (Counterclockwise)

Preceding gene: 89052985

Following gene: 89052983

Centisome position: 11.41

GC content: 63.86

Gene sequence:

>1176_bases
ATGACCAACGTCGTCATCGCCTCCGCCGCCCGAACGCCGGTTGGCAGCTTCCTCGGATCTTTCGCCAATACATCCGCCCA
TGACCTCGGCAAAACTGTGATTGAGGCTGTGGTTGAACGGGCCGGCATCGACAAGGCGGACGTGTCCGAGACCATCCTGG
GTCAGGTCCTGACCGCGGCACAGGGCCAGAACCCCGCACGCCAGGCCCACATCAACGCGGGCCTCCCGAAGGAAAGCGCC
GCTTGGTCCATCAACCAGGTCTGCGGCTCCGGTCTGCGCGCCGTGGCGCTGGGGGCACAGCATATCATGCTCGGCGATGC
CTCTGTCGTGGTCGCCGGTGGCCAGGAAAACATGTCCATGTCCCCCCACGCGGCAATGCTTCGGCAGGGCCAGAAGATGG
GCGACATGAAATATATCGACACGATGATCCGCGACGGTTTGTGGGACGCCTTCAACGGCTATCACATGGGCCAGACCGCT
GAGAACGTGGCCGAGAAGTGGCAGATCTCGCGCGAGCAGCAGGATGAATTTGCCGTCGCGTCGCAAAACAAGGCCGAAGC
CGCCCAGAAAGACGGCCGCTTTGCCGATGAAATCACGCCTGTGATCGTCCCGAACCGCAAGGGCGACATCACCGTCGACG
CGGACGAATACATCCGCCACGGCGCAACGATGGAGGCGATGGCCAAACTGCGCCCTGCCTTCACCAAGGAAGGGTCGGTC
ACTGCCGCAAACGCCTCCGGCCTGAACGATGGTGCGGCGGCCACGCTGCTGATGTCCGCTGACGATGCCGAAAAGCGGGG
CATCCAGCCGCTCGCACGCATCGCCTCCTACGCAACCGCCGGTCTCGACCCCTCGATCATGGGCGTTGGTCCGATCTATG
CGTCGCGCAAAGCGTTGGAAAAAGCCGGTTGGTCCGTGGATGATCTGGATCTGGTGGAAGCCAATGAAGCCTTCGCCGCA
CAGGCCTGCGCCGTGAACAAGGACATGGGCTGGGATCCCAGCATCGTGAACGTCAACGGCGGCGCCATCGCCATCGGCCA
CCCCATCGGCGCATCGGGCTGCCGCGTGCTGAACACGTTGCTCTTCGAAATGCAGCGCCGGGACGCGAAGAAAGGCCTCG
CCACGCTGTGCATCGGCGGCGGCATGGGCGTTGCCCTCTGCGTCGAGCGGGACTGA

Upstream 100 bases:

>100_bases
GTTGAACTTCACCCCCTTTGCGCACTATGACGCGGCGGAACTCACGCCGCGCCGGATGTCGGATCATCCGCCCAGGCGCG
CACAGGAAAGGACATCTTCC

Downstream 100 bases:

>100_bases
TCACCCTTACATCGCAGGCCGTGCGCAATAAATTTGCGCGCGGCCCTTTCTTTGCGTAACAAAGTTATAAACTTCAGAAG
GAGAGACACATGGGACGAGT

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTNVVIASAARTPVGSFLGSFANTSAHDLGKTVIEAVVERAGIDKADVSETILGQVLTAAQGQNPARQAHINAGLPKESA
AWSINQVCGSGLRAVALGAQHIMLGDASVVVAGGQENMSMSPHAAMLRQGQKMGDMKYIDTMIRDGLWDAFNGYHMGQTA
ENVAEKWQISREQQDEFAVASQNKAEAAQKDGRFADEITPVIVPNRKGDITVDADEYIRHGATMEAMAKLRPAFTKEGSV
TAANASGLNDGAAATLLMSADDAEKRGIQPLARIASYATAGLDPSIMGVGPIYASRKALEKAGWSVDDLDLVEANEAFAA
QACAVNKDMGWDPSIVNVNGGAIAIGHPIGASGCRVLNTLLFEMQRRDAKKGLATLCIGGGMGVALCVERD

Sequences:

>Translated_391_residues
MTNVVIASAARTPVGSFLGSFANTSAHDLGKTVIEAVVERAGIDKADVSETILGQVLTAAQGQNPARQAHINAGLPKESA
AWSINQVCGSGLRAVALGAQHIMLGDASVVVAGGQENMSMSPHAAMLRQGQKMGDMKYIDTMIRDGLWDAFNGYHMGQTA
ENVAEKWQISREQQDEFAVASQNKAEAAQKDGRFADEITPVIVPNRKGDITVDADEYIRHGATMEAMAKLRPAFTKEGSV
TAANASGLNDGAAATLLMSADDAEKRGIQPLARIASYATAGLDPSIMGVGPIYASRKALEKAGWSVDDLDLVEANEAFAA
QACAVNKDMGWDPSIVNVNGGAIAIGHPIGASGCRVLNTLLFEMQRRDAKKGLATLCIGGGMGVALCVERD
>Mature_390_residues
TNVVIASAARTPVGSFLGSFANTSAHDLGKTVIEAVVERAGIDKADVSETILGQVLTAAQGQNPARQAHINAGLPKESAA
WSINQVCGSGLRAVALGAQHIMLGDASVVVAGGQENMSMSPHAAMLRQGQKMGDMKYIDTMIRDGLWDAFNGYHMGQTAE
NVAEKWQISREQQDEFAVASQNKAEAAQKDGRFADEITPVIVPNRKGDITVDADEYIRHGATMEAMAKLRPAFTKEGSVT
AANASGLNDGAAATLLMSADDAEKRGIQPLARIASYATAGLDPSIMGVGPIYASRKALEKAGWSVDDLDLVEANEAFAAQ
ACAVNKDMGWDPSIVNVNGGAIAIGHPIGASGCRVLNTLLFEMQRRDAKKGLATLCIGGGMGVALCVERD

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=390, Percent_Identity=56.9230769230769, Blast_Score=461, Evalue=1e-130,
Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=41.7721518987342, Blast_Score=311, Evalue=6e-85,
Organism=Homo sapiens, GI4557237, Length=392, Percent_Identity=41.8367346938776, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI4501853, Length=398, Percent_Identity=40.9547738693467, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI4504327, Length=423, Percent_Identity=32.8605200945626, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI194353979, Length=394, Percent_Identity=31.2182741116751, Blast_Score=142, Evalue=7e-34,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=54.3367346938776, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1788554, Length=392, Percent_Identity=55.8673469387755, Blast_Score=413, Evalue=1e-116,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=41.1471321695761, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=41.7283950617284, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1788683, Length=409, Percent_Identity=33.0073349633252, Blast_Score=201, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI133906874, Length=389, Percent_Identity=40.6169665809769, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI25147385, Length=386, Percent_Identity=39.119170984456, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17535921, Length=391, Percent_Identity=37.8516624040921, Blast_Score=262, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17551802, Length=426, Percent_Identity=31.2206572769953, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17535917, Length=392, Percent_Identity=29.8469387755102, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6325229, Length=395, Percent_Identity=42.5316455696203, Blast_Score=283, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6322031, Length=394, Percent_Identity=36.2944162436548, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24655093, Length=390, Percent_Identity=56.4102564102564, Blast_Score=455, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24640423, Length=392, Percent_Identity=45.1530612244898, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI17648125, Length=389, Percent_Identity=39.8457583547558, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=32.1596244131455, Blast_Score=191, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40981; Mature: 40850

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVVIASAARTPVGSFLGSFANTSAHDLGKTVIEAVVERAGIDKADVSETILGQVLTAA
CCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
QGQNPARQAHINAGLPKESAAWSINQVCGSGLRAVALGAQHIMLGDASVVVAGGQENMSM
CCCCCHHHHCCCCCCCCCHHCCCHHHHHCCCHHEEHHCCCEEEECCCEEEEECCCCCCCC
SPHAAMLRQGQKMGDMKYIDTMIRDGLWDAFNGYHMGQTAENVAEKWQISREQQDEFAVA
CHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
SQNKAEAAQKDGRFADEITPVIVPNRKGDITVDADEYIRHGATMEAMAKLRPAFTKEGSV
CCCHHHHHHHCCCCHHHCCCEEECCCCCCEEECHHHHHHCCCHHHHHHHHHHHHCCCCCE
TAANASGLNDGAAATLLMSADDAEKRGIQPLARIASYATAGLDPSIMGVGPIYASRKALE
EECCCCCCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHH
KAGWSVDDLDLVEANEAFAAQACAVNKDMGWDPSIVNVNGGAIAIGHPIGASGCRVLNTL
HCCCCCCCCHHEECCHHHHHHHHHCCCCCCCCCCEEEECCCEEEECCCCCCHHHHHHHHH
LFEMQRRDAKKGLATLCIGGGMGVALCVERD
HHHHHHHHHHCCCEEEEECCCCEEEEEEECC
>Mature Secondary Structure 
TNVVIASAARTPVGSFLGSFANTSAHDLGKTVIEAVVERAGIDKADVSETILGQVLTAA
CCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
QGQNPARQAHINAGLPKESAAWSINQVCGSGLRAVALGAQHIMLGDASVVVAGGQENMSM
CCCCCHHHHCCCCCCCCCHHCCCHHHHHCCCHHEEHHCCCEEEECCCEEEEECCCCCCCC
SPHAAMLRQGQKMGDMKYIDTMIRDGLWDAFNGYHMGQTAENVAEKWQISREQQDEFAVA
CHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
SQNKAEAAQKDGRFADEITPVIVPNRKGDITVDADEYIRHGATMEAMAKLRPAFTKEGSV
CCCHHHHHHHCCCCHHHCCCEEECCCCCCEEECHHHHHHCCCHHHHHHHHHHHHCCCCCE
TAANASGLNDGAAATLLMSADDAEKRGIQPLARIASYATAGLDPSIMGVGPIYASRKALE
EECCCCCCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHH
KAGWSVDDLDLVEANEAFAAQACAVNKDMGWDPSIVNVNGGAIAIGHPIGASGCRVLNTL
HCCCCCCCCHHEECCHHHHHHHHHCCCCCCCCCCEEEECCCEEEECCCCCCHHHHHHHHH
LFEMQRRDAKKGLATLCIGGGMGVALCVERD
HHHHHHHHHHCCCEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8566717 [H]