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The map label for this gene is yfgF [H]

Identifier: 89052985

GI number: 89052985

Start: 492860

End: 493690

Strand: Reverse

Name: yfgF [H]

Synonym: Jann_0494

Alternate gene names: 89052985

Gene position: 493690-492860 (Counterclockwise)

Preceding gene: 89052987

Following gene: 89052984

Centisome position: 11.43

GC content: 59.21

Gene sequence:

>831_bases
ATGCGCGATGACAGCGTGGAACCGGCAGAGCCGGGGCTGGCAAGCCCCCTTGATGCGGCTATCAACCAAAGGGACCGCGG
GACCCTGGCGATGGTGCAGCAGGCGCTTGCACGGGGTGACACACGTCTGGCGTTTCAGCCCATCATACGCGCGGACGGCA
AGGGGGTCGCCTTTTATGAAGGCTTGATCCGGGTCCTTGATGAAACCGGTCGCGTGATCCCGGCCAGTGATTTCATGGGC
GCGGTGGAGGATTCGGACATCGGGCGGCAGATCGACTGCGCCGCGCTCGACATCGGCCTTCGGATGCTGCGCGACCATCC
CGGACTGCGCCTGTCCATCAACATGTCCGCCCGGTCCGTCGGATATCCCCGCTGGATGAAGATCCTGCGCACATGCCTGC
GCGATCACCCGATAGTGGTGGAACGGCTGATTCTGGAAATAACCGAAAGCTCGGTCATGATCATGCCCGAGATTGTCGCG
ATTTTTATGGAAGATTTGCAGGATAAGGGCATCACGTTCGCCTTGGATGACTTTGGCGCAGGTTATACGGCCTTCCGCCA
CTTCAAGACCTTCTACTTCGATGTCCTGAAGATTGATGGCCAGTTTACCCGCGACATCGCCCATGACGCCGACAATCAGG
TATTGTCGGAGGCCCTCATCAGTCTTGCACGTCATTTCGATATGCTTGTGGTCTCGGAATCAGTCGAAACCGCGGAAGAG
GCGAGCTGGCTTGTCTCCGCCGGCGTCGATTGCTTGCAGGGCTATTACTATGGCCCCCCCACCATGAAACTGCCTGATGT
CGGCACCAACCAGATCGCGCGCACGGGTTAG

Upstream 100 bases:

>100_bases
GCCTAAACTCCTCCAAATTCTATACAAATCCCGACCTTCATCCTTCTTTAAAGGGTCGCACGGCATCAAGCTTAAACGCG
AGGGCGATTGGAGTGAAAAG

Downstream 100 bases:

>100_bases
CCCGCTGCTGCGCATGCCAGAGGTCAGATCGCCTTGCGCGCCCTGCATATCCCCCATCCCTTCGATGCCTGAGACACCGT
GACGGTTGAACTTCACCCCC

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MRDDSVEPAEPGLASPLDAAINQRDRGTLAMVQQALARGDTRLAFQPIIRADGKGVAFYEGLIRVLDETGRVIPASDFMG
AVEDSDIGRQIDCAALDIGLRMLRDHPGLRLSINMSARSVGYPRWMKILRTCLRDHPIVVERLILEITESSVMIMPEIVA
IFMEDLQDKGITFALDDFGAGYTAFRHFKTFYFDVLKIDGQFTRDIAHDADNQVLSEALISLARHFDMLVVSESVETAEE
ASWLVSAGVDCLQGYYYGPPTMKLPDVGTNQIARTG

Sequences:

>Translated_276_residues
MRDDSVEPAEPGLASPLDAAINQRDRGTLAMVQQALARGDTRLAFQPIIRADGKGVAFYEGLIRVLDETGRVIPASDFMG
AVEDSDIGRQIDCAALDIGLRMLRDHPGLRLSINMSARSVGYPRWMKILRTCLRDHPIVVERLILEITESSVMIMPEIVA
IFMEDLQDKGITFALDDFGAGYTAFRHFKTFYFDVLKIDGQFTRDIAHDADNQVLSEALISLARHFDMLVVSESVETAEE
ASWLVSAGVDCLQGYYYGPPTMKLPDVGTNQIARTG
>Mature_276_residues
MRDDSVEPAEPGLASPLDAAINQRDRGTLAMVQQALARGDTRLAFQPIIRADGKGVAFYEGLIRVLDETGRVIPASDFMG
AVEDSDIGRQIDCAALDIGLRMLRDHPGLRLSINMSARSVGYPRWMKILRTCLRDHPIVVERLILEITESSVMIMPEIVA
IFMEDLQDKGITFALDDFGAGYTAFRHFKTFYFDVLKIDGQFTRDIAHDADNQVLSEALISLARHFDMLVVSESVETAEE
ASWLVSAGVDCLQGYYYGPPTMKLPDVGTNQIARTG

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=233, Percent_Identity=31.7596566523605, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082096, Length=237, Percent_Identity=30.8016877637131, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1786507, Length=235, Percent_Identity=31.9148936170213, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1790496, Length=236, Percent_Identity=27.1186440677966, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788502, Length=239, Percent_Identity=27.6150627615063, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787541, Length=236, Percent_Identity=27.9661016949153, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87081980, Length=125, Percent_Identity=40, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787055, Length=253, Percent_Identity=26.8774703557312, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI226510982, Length=155, Percent_Identity=34.8387096774194, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081845, Length=246, Percent_Identity=24.7967479674797, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081743, Length=233, Percent_Identity=25.3218884120172, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081921, Length=242, Percent_Identity=26.8595041322314, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787410, Length=235, Percent_Identity=24.2553191489362, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789650, Length=245, Percent_Identity=22.4489795918367, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 30558; Mature: 30558

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDDSVEPAEPGLASPLDAAINQRDRGTLAMVQQALARGDTRLAFQPIIRADGKGVAFYE
CCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCEEHHHHHHCCCCCHHHHH
GLIRVLDETGRVIPASDFMGAVEDSDIGRQIDCAALDIGLRMLRDHPGLRLSINMSARSV
HHHHHHHHCCCEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
GYPRWMKILRTCLRDHPIVVERLILEITESSVMIMPEIVAIFMEDLQDKGITFALDDFGA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCC
GYTAFRHFKTFYFDVLKIDGQFTRDIAHDADNQVLSEALISLARHFDMLVVSESVETAEE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHH
ASWLVSAGVDCLQGYYYGPPTMKLPDVGTNQIARTG
HHHHHHHCHHHHCCEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRDDSVEPAEPGLASPLDAAINQRDRGTLAMVQQALARGDTRLAFQPIIRADGKGVAFYE
CCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCEEHHHHHHCCCCCHHHHH
GLIRVLDETGRVIPASDFMGAVEDSDIGRQIDCAALDIGLRMLRDHPGLRLSINMSARSV
HHHHHHHHCCCEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCC
GYPRWMKILRTCLRDHPIVVERLILEITESSVMIMPEIVAIFMEDLQDKGITFALDDFGA
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCC
GYTAFRHFKTFYFDVLKIDGQFTRDIAHDADNQVLSEALISLARHFDMLVVSESVETAEE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHH
ASWLVSAGVDCLQGYYYGPPTMKLPDVGTNQIARTG
HHHHHHHCHHHHCCEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]