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The map label for this gene is ispE

Identifier: 89052977

GI number: 89052977

Start: 485706

End: 486536

Strand: Direct

Name: ispE

Synonym: Jann_0486

Alternate gene names: 89052977

Gene position: 485706-486536 (Clockwise)

Preceding gene: 89052976

Following gene: 89052979

Centisome position: 11.25

GC content: 68.59

Gene sequence:

>831_bases
GTGAGCGCCGCCACCTTCCGGGGATATGCGCCCGCCAAGGTCAACCTCGCGCTCCATGTGACGGGGCGGCGCGTTGATGG
ATATCATGAGCTGGATAGTCTGGTCGTCTTCGCGGGTGTGGGGGACCGGCTGGAGATCGCGCCTGCCGATGCGTTGTCGT
TGACGGTCACCGGGCCAAGGGCCGAAGGGGTGCCCGATGACGCGCGCAATCTGGTGTGGAAGGCTGCGGATTGGTTGGCC
CCCGGGCGTGGCGCGGCGATGACCCTGGACAAGCATCTGCCCCATGCGGGTGGAATTGGCGGCGGATCTGCCGATGCGGC
CTGTGCGCTACGGGGGTTGGCCGAGATCTGGGACGTCGATGTGCCCGACGGGGCCGAGGCGCTTGGCGCGGACGTGCCCG
TCTGCCTGCACGGACAGCCCGTTCGGATGCGCGGGATCGGCGATGTGCTGGATGCCGTGCCCCCGCTGCCGCCGATGTGG
ATCGTACTGGTCAACGCAGGCGTCGAGGTGCCGACGGGGGCCGTCTTCAAGGCGATGGAGCGGGTGGACAATCCGCCGCT
GCCCGCGCCCGCCTGGGATGGGTTCGACAGCTTCCTCGTGTGGCTAGAGCGGACCCGGAATGATATGGAATCCTCCGCGC
GCAGCCAGGCCCCGGTGATCGGTATGGTGCTGGAACGCCTGCGCGCCCTTCCCGGATGCCGGTTCACCCGGATGAGCGGG
TCCGGCGGCACATGCTTTGGCCTGTTCGAGACCGAGATCGCCGCCCGCGATGCGGCCCTGGGTCTGCCCAAGCACTGGTG
GGTGACCTACGCGCCCGTCTTGAGGGCATAG

Upstream 100 bases:

>100_bases
ATCCCGAGGAGGAGGACGCCGTGCGGATCCGGCGCAAGCTGGAAGTCGGCCTTGACGTTGTGCTGGAGGAAGAAGGCGGC
GTGGGCGTTCTGGAAGAAGA

Downstream 100 bases:

>100_bases
CCCCCGGCGGGGTAAATCCCACCCTACGGATGCTGAAAGCGCGGGCCTAATTCAGGCGCGCCACCACGTAATCTGCCAGA
TCGGCCAGCATGTCGCGAAG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSAATFRGYAPAKVNLALHVTGRRVDGYHELDSLVVFAGVGDRLEIAPADALSLTVTGPRAEGVPDDARNLVWKAADWLA
PGRGAAMTLDKHLPHAGGIGGGSADAACALRGLAEIWDVDVPDGAEALGADVPVCLHGQPVRMRGIGDVLDAVPPLPPMW
IVLVNAGVEVPTGAVFKAMERVDNPPLPAPAWDGFDSFLVWLERTRNDMESSARSQAPVIGMVLERLRALPGCRFTRMSG
SGGTCFGLFETEIAARDAALGLPKHWWVTYAPVLRA

Sequences:

>Translated_276_residues
MSAATFRGYAPAKVNLALHVTGRRVDGYHELDSLVVFAGVGDRLEIAPADALSLTVTGPRAEGVPDDARNLVWKAADWLA
PGRGAAMTLDKHLPHAGGIGGGSADAACALRGLAEIWDVDVPDGAEALGADVPVCLHGQPVRMRGIGDVLDAVPPLPPMW
IVLVNAGVEVPTGAVFKAMERVDNPPLPAPAWDGFDSFLVWLERTRNDMESSARSQAPVIGMVLERLRALPGCRFTRMSG
SGGTCFGLFETEIAARDAALGLPKHWWVTYAPVLRA
>Mature_275_residues
SAATFRGYAPAKVNLALHVTGRRVDGYHELDSLVVFAGVGDRLEIAPADALSLTVTGPRAEGVPDDARNLVWKAADWLAP
GRGAAMTLDKHLPHAGGIGGGSADAACALRGLAEIWDVDVPDGAEALGADVPVCLHGQPVRMRGIGDVLDAVPPLPPMWI
VLVNAGVEVPTGAVFKAMERVDNPPLPAPAWDGFDSFLVWLERTRNDMESSARSQAPVIGMVLERLRALPGCRFTRMSGS
GGTCFGLFETEIAARDAALGLPKHWWVTYAPVLRA

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=269, Percent_Identity=34.5724907063197, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_JANSC (Q28V59)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508428.1
- ProteinModelPortal:   Q28V59
- SMR:   Q28V59
- STRING:   Q28V59
- GeneID:   3932927
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0486
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   PAVMVNP
- PhylomeDB:   Q28V59
- BioCyc:   JSP290400:JANN_0486-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29214; Mature: 29083

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: ACT_SITE 13-13 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAATFRGYAPAKVNLALHVTGRRVDGYHELDSLVVFAGVGDRLEIAPADALSLTVTGPR
CCCCCCCCCCCEEEEEEEEEECCCCCCHHHHCCEEEEECCCCEEEECCCCEEEEEEECCC
AEGVPDDARNLVWKAADWLAPGRGAAMTLDKHLPHAGGIGGGSADAACALRGLAEIWDVD
CCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
VPDGAEALGADVPVCLHGQPVRMRGIGDVLDAVPPLPPMWIVLVNAGVEVPTGAVFKAME
CCCCHHHHCCCCCEEECCCCEEECCHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHH
RVDNPPLPAPAWDGFDSFLVWLERTRNDMESSARSQAPVIGMVLERLRALPGCRFTRMSG
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECC
SGGTCFGLFETEIAARDAALGLPKHWWVTYAPVLRA
CCCEEEEEEHHHHHHHHHHCCCCCHHHEEHHHHHCC
>Mature Secondary Structure 
SAATFRGYAPAKVNLALHVTGRRVDGYHELDSLVVFAGVGDRLEIAPADALSLTVTGPR
CCCCCCCCCCEEEEEEEEEECCCCCCHHHHCCEEEEECCCCEEEECCCCEEEEEEECCC
AEGVPDDARNLVWKAADWLAPGRGAAMTLDKHLPHAGGIGGGSADAACALRGLAEIWDVD
CCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
VPDGAEALGADVPVCLHGQPVRMRGIGDVLDAVPPLPPMWIVLVNAGVEVPTGAVFKAME
CCCCHHHHCCCCCEEECCCCEEECCHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHH
RVDNPPLPAPAWDGFDSFLVWLERTRNDMESSARSQAPVIGMVLERLRALPGCRFTRMSG
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECC
SGGTCFGLFETEIAARDAALGLPKHWWVTYAPVLRA
CCCEEEEEEHHHHHHHHHHCCCCCHHHEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA