Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 89052959

GI number: 89052959

Start: 466988

End: 467482

Strand: Direct

Name: def [H]

Synonym: Jann_0468

Alternate gene names: 89052959

Gene position: 466988-467482 (Clockwise)

Preceding gene: 89052958

Following gene: 89052960

Centisome position: 10.81

GC content: 62.83

Gene sequence:

>495_bases
ATGCCGATTAGGCCTTTCGTCCCCTATCCAGACAAGCGTTTGCGCACGATGGCAGAGACCGTAGGACCTGTCACCGACGC
CCATCGGGAGATCTGGCAGGACATGATCGACACGATGGATGCGATGCCGGGCGTCGGCCTGGCCGCGCCACAGATCGGGG
TCATGCTGCGCCTCGCGGTCGTGGATGCCAGCGATGACCGCGGTCAAGCGATCCGCATGGCCGACCCGGAGATCATCAGC
GCCTCAGATGAGATGAACACCTACCCTGAGGGGTCGCCAAACCTGCCGGGTGTGACGGCCAAAATCACGCGTCCCGCCCG
CGTCACCGTCGCTTTCACCGATCATATGGGCCTGCGTGTGCGGCAGGAGTTCGTGGACCTCTGGGCCACGTCCGTCCAGC
ACCAGATCGACCATCTGGCGGGCAAAGTGTATGTCGATCACCTCAGCCGAACGAAGCGCGAGATGGTGATCAAGAAATCC
CGGAGGGGCAGCTGA

Upstream 100 bases:

>100_bases
AACCTTGCGCGCTGTATCCTCCATGAACTGGACCACCTGGACGGAACGGTCATCTTCGACCACCAATCCCCAGCGCGGCG
CGCAGAACTGGAAGCGGCTT

Downstream 100 bases:

>100_bases
TGCGGATCATCTTCATGGGGACGCCCGACTTTTCCGTCCCGGTTCTGGACGCGCTGGTGGCCGCAGAGCATGAGGTCGTC
GCGGTCTATTCGCAACCTCC

Product: formylmethionine deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MPIRPFVPYPDKRLRTMAETVGPVTDAHREIWQDMIDTMDAMPGVGLAAPQIGVMLRLAVVDASDDRGQAIRMADPEIIS
ASDEMNTYPEGSPNLPGVTAKITRPARVTVAFTDHMGLRVRQEFVDLWATSVQHQIDHLAGKVYVDHLSRTKREMVIKKS
RRGS

Sequences:

>Translated_164_residues
MPIRPFVPYPDKRLRTMAETVGPVTDAHREIWQDMIDTMDAMPGVGLAAPQIGVMLRLAVVDASDDRGQAIRMADPEIIS
ASDEMNTYPEGSPNLPGVTAKITRPARVTVAFTDHMGLRVRQEFVDLWATSVQHQIDHLAGKVYVDHLSRTKREMVIKKS
RRGS
>Mature_163_residues
PIRPFVPYPDKRLRTMAETVGPVTDAHREIWQDMIDTMDAMPGVGLAAPQIGVMLRLAVVDASDDRGQAIRMADPEIISA
SDEMNTYPEGSPNLPGVTAKITRPARVTVAFTDHMGLRVRQEFVDLWATSVQHQIDHLAGKVYVDHLSRTKREMVIKKSR
RGS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=162, Percent_Identity=32.7160493827161, Blast_Score=94, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=28.3132530120482, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18290; Mature: 18159

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIRPFVPYPDKRLRTMAETVGPVTDAHREIWQDMIDTMDAMPGVGLAAPQIGVMLRLAV
CCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEEEE
VDASDDRGQAIRMADPEIISASDEMNTYPEGSPNLPGVTAKITRPARVTVAFTDHMGLRV
EECCCCCCCEEEECCCCEECCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCHHH
RQEFVDLWATSVQHQIDHLAGKVYVDHLSRTKREMVIKKSRRGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PIRPFVPYPDKRLRTMAETVGPVTDAHREIWQDMIDTMDAMPGVGLAAPQIGVMLRLAV
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEEEE
VDASDDRGQAIRMADPEIISASDEMNTYPEGSPNLPGVTAKITRPARVTVAFTDHMGLRV
EECCCCCCCEEEECCCCEECCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEEECCCCHHH
RQEFVDLWATSVQHQIDHLAGKVYVDHLSRTKREMVIKKSRRGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA