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The map label for this gene is def [H]

Identifier: 89052958

GI number: 89052958

Start: 466513

End: 466998

Strand: Direct

Name: def [H]

Synonym: Jann_0467

Alternate gene names: 89052958

Gene position: 466513-466998 (Clockwise)

Preceding gene: 89052957

Following gene: 89052959

Centisome position: 10.8

GC content: 62.55

Gene sequence:

>486_bases
GTGAGTGTCCGGCGGGTCCTGCTGTGGCCCGATCCGCTGTTGTCGGTCGTTTGCGATGCGGTCGTGGCCGTTGATCCCGA
GTTGATCAAAGACCTGTTCGATACGATGTACGCCGCGAAAGGACGGGGCCTCGCCGCGCCACAGATCGGTGTGTTGCAGC
GGGTCTTCGTGGTGGATGTGACTTGGAAAGAGGGCGCGCGCGATCCGCGTGTGTTCATCAACCCGGTGGTGCGGGACAGC
GGCGAGGACATGCGCAGCATGGAGGAGCAGTGCCTGTCCATTCCCGACCTGCCGATGCAAGTTACACGGCCCACCACCCT
CACGCTTGACTGGGAAACGCCCGAAGGGCGCAAAACAACGGGCACGTTCGACGGCAACCTTGCGCGCTGTATCCTCCATG
AACTGGACCACCTGGACGGAACGGTCATCTTCGACCACCAATCCCCAGCGCGGCGCGCAGAACTGGAAGCGGCTTATGCC
GATTAG

Upstream 100 bases:

>100_bases
TGGAGGGAAAGCTTTTCATCGACTACCTCAGCGGGCTGAAGCGGCAGCTGATCACGCGCAAGATGGTGAAGTTGAAGCGC
GACCGAGCGCGCGAAGGCGC

Downstream 100 bases:

>100_bases
GCCTTTCGTCCCCTATCCAGACAAGCGTTTGCGCACGATGGCAGAGACCGTAGGACCTGTCACCGACGCCCATCGGGAGA
TCTGGCAGGACATGATCGAC

Product: formylmethionine deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MSVRRVLLWPDPLLSVVCDAVVAVDPELIKDLFDTMYAAKGRGLAAPQIGVLQRVFVVDVTWKEGARDPRVFINPVVRDS
GEDMRSMEEQCLSIPDLPMQVTRPTTLTLDWETPEGRKTTGTFDGNLARCILHELDHLDGTVIFDHQSPARRAELEAAYA
D

Sequences:

>Translated_161_residues
MSVRRVLLWPDPLLSVVCDAVVAVDPELIKDLFDTMYAAKGRGLAAPQIGVLQRVFVVDVTWKEGARDPRVFINPVVRDS
GEDMRSMEEQCLSIPDLPMQVTRPTTLTLDWETPEGRKTTGTFDGNLARCILHELDHLDGTVIFDHQSPARRAELEAAYA
D
>Mature_160_residues
SVRRVLLWPDPLLSVVCDAVVAVDPELIKDLFDTMYAAKGRGLAAPQIGVLQRVFVVDVTWKEGARDPRVFINPVVRDSG
EDMRSMEEQCLSIPDLPMQVTRPTTLTLDWETPEGRKTTGTFDGNLARCILHELDHLDGTVIFDHQSPARRAELEAAYAD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=157, Percent_Identity=40.7643312101911, Blast_Score=96, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 17989; Mature: 17858

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRRVLLWPDPLLSVVCDAVVAVDPELIKDLFDTMYAAKGRGLAAPQIGVLQRVFVVDV
CCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEEEEE
TWKEGARDPRVFINPVVRDSGEDMRSMEEQCLSIPDLPMQVTRPTTLTLDWETPEGRKTT
EHHCCCCCCEEEEEHHCCCCCHHHHHHHHHHCCCCCCCEEECCCEEEEEECCCCCCCCCC
GTFDGNLARCILHELDHLDGTVIFDHQSPARRAELEAAYAD
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCC
>Mature Secondary Structure 
SVRRVLLWPDPLLSVVCDAVVAVDPELIKDLFDTMYAAKGRGLAAPQIGVLQRVFVVDV
CCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHEEEEEEEE
TWKEGARDPRVFINPVVRDSGEDMRSMEEQCLSIPDLPMQVTRPTTLTLDWETPEGRKTT
EHHCCCCCCEEEEEHHCCCCCHHHHHHHHHHCCCCCCCEEECCCEEEEEECCCCCCCCCC
GTFDGNLARCILHELDHLDGTVIFDHQSPARRAELEAAYAD
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA