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The map label for this gene is mscL

Identifier: 89052948

GI number: 89052948

Start: 458997

End: 459488

Strand: Direct

Name: mscL

Synonym: Jann_0457

Alternate gene names: 89052948

Gene position: 458997-459488 (Clockwise)

Preceding gene: 89052946

Following gene: 89052950

Centisome position: 10.63

GC content: 55.49

Gene sequence:

>492_bases
GTGTGCGAATGCGCACGAAAATCAATCCCTTGCGAACAGGAACTGAAAGATTGGGACACTCACATGATTAACGAATTCAA
GGACTTCATTGCCAAGGGCAATGTGATGGACATGGCCGTCGGTATCATTATCGGCGCGGCATTCACCGCAATCGTCACCT
CGCTGGTGGGCGATCTGATCAATCCGATCATCGCACTGTTTACCGGTGGCATGGATTTCTCGGGATGGTTCTATGTGCTG
GGTGACGGCGAATGCGCGTCGGTCGCGGCCTGTACGGAGGCAGGGATTTCCGTCTTTGCCATCGGCAACTTCCTGATGGC
GATCATCAACTTCCTGATCATCGCCTTCGTTGTCTTCATGCTGGTGAAGATGGTCAACCGTGTGAAAGCTGCCGCAGAGA
AGCCCGATGAGGTTGCCCCAGAGGTGGAGACGGGCCCGTCCGAGATCGACCTGCTGATCGAGATCCGCGATGGCTTGAAG
GCCCGCGGCTAA

Upstream 100 bases:

>100_bases
CGGTCATTGGCTTATCCTATGCTTCGAAGTGTTCATGCGCAGATATGTCCCCTTGCCTCACCGTTCCAGCCCGGCGAGTG
TGTCGCCGCGAGAGGGAGCC

Downstream 100 bases:

>100_bases
CAACCGTCCGTTCAAGACGCGACGGGGCCGAGGGTGTGACACCTCGGCCCCATCGGCGCGCTAGCCTTTGATCGCGAGTG
ACGGGGATAGCACATCAAGC

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MCECARKSIPCEQELKDWDTHMINEFKDFIAKGNVMDMAVGIIIGAAFTAIVTSLVGDLINPIIALFTGGMDFSGWFYVL
GDGECASVAACTEAGISVFAIGNFLMAIINFLIIAFVVFMLVKMVNRVKAAAEKPDEVAPEVETGPSEIDLLIEIRDGLK
ARG

Sequences:

>Translated_163_residues
MCECARKSIPCEQELKDWDTHMINEFKDFIAKGNVMDMAVGIIIGAAFTAIVTSLVGDLINPIIALFTGGMDFSGWFYVL
GDGECASVAACTEAGISVFAIGNFLMAIINFLIIAFVVFMLVKMVNRVKAAAEKPDEVAPEVETGPSEIDLLIEIRDGLK
ARG
>Mature_163_residues
MCECARKSIPCEQELKDWDTHMINEFKDFIAKGNVMDMAVGIIIGAAFTAIVTSLVGDLINPIIALFTGGMDFSGWFYVL
GDGECASVAACTEAGISVFAIGNFLMAIINFLIIAFVVFMLVKMVNRVKAAAEKPDEVAPEVETGPSEIDLLIEIRDGLK
ARG

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=143, Percent_Identity=39.8601398601399, Blast_Score=98, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 17585; Mature: 17585

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCECARKSIPCEQELKDWDTHMINEFKDFIAKGNVMDMAVGIIIGAAFTAIVTSLVGDLI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NPIIALFTGGMDFSGWFYVLGDGECASVAACTEAGISVFAIGNFLMAIINFLIIAFVVFM
HHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
LVKMVNRVKAAAEKPDEVAPEVETGPSEIDLLIEIRDGLKARG
HHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHEECCCCCCCC
>Mature Secondary Structure
MCECARKSIPCEQELKDWDTHMINEFKDFIAKGNVMDMAVGIIIGAAFTAIVTSLVGDLI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NPIIALFTGGMDFSGWFYVLGDGECASVAACTEAGISVFAIGNFLMAIINFLIIAFVVFM
HHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
LVKMVNRVKAAAEKPDEVAPEVETGPSEIDLLIEIRDGLKARG
HHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA