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The map label for this gene is yieH [H]

Identifier: 89052933

GI number: 89052933

Start: 445582

End: 446238

Strand: Direct

Name: yieH [H]

Synonym: Jann_0442

Alternate gene names: 89052933

Gene position: 445582-446238 (Clockwise)

Preceding gene: 89052932

Following gene: 89052934

Centisome position: 10.32

GC content: 60.27

Gene sequence:

>657_bases
ATGATACCTGACCTTGTCATCTTCGATTGCGATGGAGTGCTGGTGGACAGCGAGCCCTTGTCCAATCAGGTTACCGTCGA
TGCGCTGGCAGACATGGGTATTCACATGAGCCTGGACGAGATTATGGCGATGTTCGTTGGCAAGAGCATGGCTCAGGTTG
CGGCCGGTGTGCGTGCCATGGGTGCACCACTGCCCGGCTCGGATGCGGAGTGGATCGACACCCTATATACCGAGACCTAC
GCGCGGCTGCGCCAAGGGGTGGATCCCATCCCCGGTGTCGTGGGCGTGCTTGACGCGTTGGACACCGCAGGCGTGCCCTA
TTGCGTCGCGTCCAACGGGTCCGATGAGAAGATGGACATCACCCTTGGCGGCACCGGCATGGCGGAGCGTTTCAAGGGCA
AACGGTTTTCCGCCCACACATTGGGTGTGTCAAAACCGGACCCGGAGTTGTTCTTCATCGCGGCGAAATACATGAGCGTC
GTGCCGTCGCGCGCGGTGGTGATCGAGGACAGCCCATCGGGAGCGTTAGGCGCACAGCGGGCTGGGATGCGGTGCTTTGG
CTATGCACCAGAGGGAGGGGACGCTTTGGAAGAGGTGGGCGCACACGTCTTTCAATCCATGAACGATCTACCAGCCCTGC
TCGGGATTAAGGACTAA

Upstream 100 bases:

>100_bases
TGGTGTGGGAAGAAAGCGCCCCGCCCATTATTCCCGCGCCGTTTCAGATGCTTGACCAACGCGGCTACGGCCAAACCACC
ATAACCATCCTGAGGTTCCC

Downstream 100 bases:

>100_bases
TCCACAAGATGTTGGCGTCCCGGGTCTTGTTTACGGAATCTCAATATGGGACCATCTAGATAACAACGAGCATTTAGATA
AAAATCAACAGGCTGCCCCA

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MIPDLVIFDCDGVLVDSEPLSNQVTVDALADMGIHMSLDEIMAMFVGKSMAQVAAGVRAMGAPLPGSDAEWIDTLYTETY
ARLRQGVDPIPGVVGVLDALDTAGVPYCVASNGSDEKMDITLGGTGMAERFKGKRFSAHTLGVSKPDPELFFIAAKYMSV
VPSRAVVIEDSPSGALGAQRAGMRCFGYAPEGGDALEEVGAHVFQSMNDLPALLGIKD

Sequences:

>Translated_218_residues
MIPDLVIFDCDGVLVDSEPLSNQVTVDALADMGIHMSLDEIMAMFVGKSMAQVAAGVRAMGAPLPGSDAEWIDTLYTETY
ARLRQGVDPIPGVVGVLDALDTAGVPYCVASNGSDEKMDITLGGTGMAERFKGKRFSAHTLGVSKPDPELFFIAAKYMSV
VPSRAVVIEDSPSGALGAQRAGMRCFGYAPEGGDALEEVGAHVFQSMNDLPALLGIKD
>Mature_218_residues
MIPDLVIFDCDGVLVDSEPLSNQVTVDALADMGIHMSLDEIMAMFVGKSMAQVAAGVRAMGAPLPGSDAEWIDTLYTETY
ARLRQGVDPIPGVVGVLDALDTAGVPYCVASNGSDEKMDITLGGTGMAERFKGKRFSAHTLGVSKPDPELFFIAAKYMSV
VPSRAVVIEDSPSGALGAQRAGMRCFGYAPEGGDALEEVGAHVFQSMNDLPALLGIKD

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=211, Percent_Identity=31.7535545023697, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082080, Length=196, Percent_Identity=27.0408163265306, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 22916; Mature: 22916

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPDLVIFDCDGVLVDSEPLSNQVTVDALADMGIHMSLDEIMAMFVGKSMAQVAAGVRAM
CCCCEEEEECCCEEECCCCCCCCEEHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHH
GAPLPGSDAEWIDTLYTETYARLRQGVDPIPGVVGVLDALDTAGVPYCVASNGSDEKMDI
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEEE
TLGGTGMAERFKGKRFSAHTLGVSKPDPELFFIAAKYMSVVPSRAVVIEDSPSGALGAQR
EECCCCHHHHHCCCCCEEEECCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCCCCHHH
AGMRCFGYAPEGGDALEEVGAHVFQSMNDLPALLGIKD
CCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure
MIPDLVIFDCDGVLVDSEPLSNQVTVDALADMGIHMSLDEIMAMFVGKSMAQVAAGVRAM
CCCCEEEEECCCEEECCCCCCCCEEHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHHH
GAPLPGSDAEWIDTLYTETYARLRQGVDPIPGVVGVLDALDTAGVPYCVASNGSDEKMDI
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEEE
TLGGTGMAERFKGKRFSAHTLGVSKPDPELFFIAAKYMSVVPSRAVVIEDSPSGALGAQR
EECCCCHHHHHCCCCCEEEECCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCCCCHHH
AGMRCFGYAPEGGDALEEVGAHVFQSMNDLPALLGIKD
CCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]