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The map label for this gene is thcD [H]

Identifier: 89052931

GI number: 89052931

Start: 443809

End: 445017

Strand: Direct

Name: thcD [H]

Synonym: Jann_0440

Alternate gene names: 89052931

Gene position: 443809-445017 (Clockwise)

Preceding gene: 89052929

Following gene: 89052932

Centisome position: 10.28

GC content: 62.2

Gene sequence:

>1209_bases
ATGGACAGTATTATCGTCATCGGAGCGGGTCAGGCGGGCGCGTCTTGCGTTGCCAAACTGCGGGCCGAAGGATTTGAGGG
CAAGATTACCCTTATAGGTGATGAGCCGGTGCCACCCTATCAGCGCCCACCTCTCTCCAAGGCTTATCTGCTTGGCGATA
TGGCGCTGGAACGGCTCTTTCTGCGGCCCGAGGCCTGGTATGCGGATAATGAGGTCGAACTGCGCTTGGGTGCGCCTGTG
GACGCGATTGACGCCGCTGAAAAGACGATCAGTGTGGAGGGCGACGTGCTGCCCTACGAGGCCCTCGTGCTGGCAACAGG
ATCGACGCCCCGTCGCTTGCCTGCCAAGATTGGCGGCGATCTGGGGGGCGTCCACGTGGTGCGCACGCTGGCGGATGTCG
ACGCGATGGAGCCCGAGGTGACAGGCAATCGCCGCGCGCTGATCGTGGGTGGGGGGTATATCGGTCTGGAGGCGGCCGCC
GTTGCCCGCAAACGCGGCATGGAGGTCACGCTGATCGAGGCCGCACCGCGTATTCTGGGCCGGGTCGCGGCGTCAGAGAC
GGCGGATTATTTCCGCGATGTGCACCGCGCCAATGGTGTGAGAATTCTGGAAGGCGTCGGCCTTGAGTGTATCAACGGTG
TGGATGGTCGCGTGGTCGGCGCGATGCTGACCAATGGCGAGGAACATCCCTATGACGTGGTGATTGCAGGCATCGGGATC
GTGCCCAACGACAGCCCCGCAGAGATGGCGGGCGTGACGCTGGACAACGGTATCGCCACGGACCTACAGGGGCGCACCAG
CGACCCAAGCATTTGGGCCGCAGGTGATTGCGCGTCGTTGCCGTTCCGGGGTGAGCGCATCCGGTTGGAGTCTGTGCAGA
ACGCGATTGATCAGGCGGAGGCTGTGGCGAAGAACATCCTCGGTGCGACGGAGGATTACGTGCCCAAACCCTGGTTCTGG
TCGGATCAATACGACGTCAAATTACAGATTGCCGGATTGAATACGGGCTACGACAAGGTCGTCGTTCGGGACGGTGGGGC
GGCGCGGTCCCACTGGTATTACAACGGCGACACATTGCTGGCCGTAGACGCGATGAACGATCCGCGTGGCTACATGATCG
GCAAGCGGTTGATTGAGGCTGGAAAGTCGCCGTCGGCAGACATCGTGGCGGACCCGGACAGCGACCTTAAGCCGTTGCTC
AAGGCATGA

Upstream 100 bases:

>100_bases
CGCCCATGGAAAGTGTCATATGTCGGTGCTCCTGCGCTTGTACCAATGTTCCTCTGGCATATAGGGTCTGGCCGGATAAG
GGCCAGCGGGGAACAGCGTC

Downstream 100 bases:

>100_bases
GGCCCGCACGATGAGAATCGTGGGGGGTCGGCTGCGCGGCAAGAAGTTGGCGGATGTGGGCACGGGCGACGCTGCGGCGC
ATCTGCGCCCCACCACGGAT

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MDSIIVIGAGQAGASCVAKLRAEGFEGKITLIGDEPVPPYQRPPLSKAYLLGDMALERLFLRPEAWYADNEVELRLGAPV
DAIDAAEKTISVEGDVLPYEALVLATGSTPRRLPAKIGGDLGGVHVVRTLADVDAMEPEVTGNRRALIVGGGYIGLEAAA
VARKRGMEVTLIEAAPRILGRVAASETADYFRDVHRANGVRILEGVGLECINGVDGRVVGAMLTNGEEHPYDVVIAGIGI
VPNDSPAEMAGVTLDNGIATDLQGRTSDPSIWAAGDCASLPFRGERIRLESVQNAIDQAEAVAKNILGATEDYVPKPWFW
SDQYDVKLQIAGLNTGYDKVVVRDGGAARSHWYYNGDTLLAVDAMNDPRGYMIGKRLIEAGKSPSADIVADPDSDLKPLL
KA

Sequences:

>Translated_402_residues
MDSIIVIGAGQAGASCVAKLRAEGFEGKITLIGDEPVPPYQRPPLSKAYLLGDMALERLFLRPEAWYADNEVELRLGAPV
DAIDAAEKTISVEGDVLPYEALVLATGSTPRRLPAKIGGDLGGVHVVRTLADVDAMEPEVTGNRRALIVGGGYIGLEAAA
VARKRGMEVTLIEAAPRILGRVAASETADYFRDVHRANGVRILEGVGLECINGVDGRVVGAMLTNGEEHPYDVVIAGIGI
VPNDSPAEMAGVTLDNGIATDLQGRTSDPSIWAAGDCASLPFRGERIRLESVQNAIDQAEAVAKNILGATEDYVPKPWFW
SDQYDVKLQIAGLNTGYDKVVVRDGGAARSHWYYNGDTLLAVDAMNDPRGYMIGKRLIEAGKSPSADIVADPDSDLKPLL
KA
>Mature_402_residues
MDSIIVIGAGQAGASCVAKLRAEGFEGKITLIGDEPVPPYQRPPLSKAYLLGDMALERLFLRPEAWYADNEVELRLGAPV
DAIDAAEKTISVEGDVLPYEALVLATGSTPRRLPAKIGGDLGGVHVVRTLADVDAMEPEVTGNRRALIVGGGYIGLEAAA
VARKRGMEVTLIEAAPRILGRVAASETADYFRDVHRANGVRILEGVGLECINGVDGRVVGAMLTNGEEHPYDVVIAGIGI
VPNDSPAEMAGVTLDNGIATDLQGRTSDPSIWAAGDCASLPFRGERIRLESVQNAIDQAEAVAKNILGATEDYVPKPWFW
SDQYDVKLQIAGLNTGYDKVVVRDGGAARSHWYYNGDTLLAVDAMNDPRGYMIGKRLIEAGKSPSADIVADPDSDLKPLL
KA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=398, Percent_Identity=27.8894472361809, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI65787454, Length=398, Percent_Identity=27.8894472361809, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI21389617, Length=375, Percent_Identity=28, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI4757732, Length=340, Percent_Identity=24.7058823529412, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI22202629, Length=340, Percent_Identity=24.7058823529412, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1788892, Length=403, Percent_Identity=36.2282878411911, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1789065, Length=325, Percent_Identity=29.8461538461538, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789765, Length=283, Percent_Identity=31.095406360424, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17559934, Length=346, Percent_Identity=27.7456647398844, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI281359715, Length=402, Percent_Identity=27.6119402985075, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI281359713, Length=402, Percent_Identity=27.6119402985075, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24639250, Length=402, Percent_Identity=27.6119402985075, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI18543267, Length=402, Percent_Identity=27.6119402985075, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24639252, Length=402, Percent_Identity=27.6119402985075, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24639257, Length=402, Percent_Identity=27.6119402985075, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24585130, Length=406, Percent_Identity=24.6305418719212, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24581020, Length=359, Percent_Identity=24.5125348189415, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28573993, Length=359, Percent_Identity=24.5125348189415, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 42739; Mature: 42739

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSIIVIGAGQAGASCVAKLRAEGFEGKITLIGDEPVPPYQRPPLSKAYLLGDMALERLF
CCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
LRPEAWYADNEVELRLGAPVDAIDAAEKTISVEGDVLPYEALVLATGSTPRRLPAKIGGD
CCCCCEECCCEEEEEECCCCCHHHCCHHEEEECCCCCCEEEEEEECCCCCCCCHHHHCCC
LGGVHVVRTLADVDAMEPEVTGNRRALIVGGGYIGLEAAAVARKRGMEVTLIEAAPRILG
CCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEECHHHHHHHHHCCCEEEEEHHHHHHHH
RVAASETADYFRDVHRANGVRILEGVGLECINGVDGRVVGAMLTNGEEHPYDVVIAGIGI
HHHHHHHHHHHHHHHHCCCEEEEECCCHHEECCCCCEEEEEEECCCCCCCEEEEEEEEEE
VPNDSPAEMAGVTLDNGIATDLQGRTSDPSIWAAGDCASLPFRGERIRLESVQNAIDQAE
CCCCCCHHHCCEEECCCCEECCCCCCCCCCEEECCCCCCCCCCCCEEEHHHHHHHHHHHH
AVAKNILGATEDYVPKPWFWSDQYDVKLQIAGLNTGYDKVVVRDGGAARSHWYYNGDTLL
HHHHHHCCCCHHCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCEEECCCEEE
AVDAMNDPRGYMIGKRLIEAGKSPSADIVADPDSDLKPLLKA
EEECCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCHHHHHCC
>Mature Secondary Structure
MDSIIVIGAGQAGASCVAKLRAEGFEGKITLIGDEPVPPYQRPPLSKAYLLGDMALERLF
CCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
LRPEAWYADNEVELRLGAPVDAIDAAEKTISVEGDVLPYEALVLATGSTPRRLPAKIGGD
CCCCCEECCCEEEEEECCCCCHHHCCHHEEEECCCCCCEEEEEEECCCCCCCCHHHHCCC
LGGVHVVRTLADVDAMEPEVTGNRRALIVGGGYIGLEAAAVARKRGMEVTLIEAAPRILG
CCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEECHHHHHHHHHCCCEEEEEHHHHHHHH
RVAASETADYFRDVHRANGVRILEGVGLECINGVDGRVVGAMLTNGEEHPYDVVIAGIGI
HHHHHHHHHHHHHHHHCCCEEEEECCCHHEECCCCCEEEEEEECCCCCCCEEEEEEEEEE
VPNDSPAEMAGVTLDNGIATDLQGRTSDPSIWAAGDCASLPFRGERIRLESVQNAIDQAE
CCCCCCHHHCCEEECCCCEECCCCCCCCCCEEECCCCCCCCCCCCEEEHHHHHHHHHHHH
AVAKNILGATEDYVPKPWFWSDQYDVKLQIAGLNTGYDKVVVRDGGAARSHWYYNGDTLL
HHHHHHCCCCHHCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCCEEECCCEEE
AVDAMNDPRGYMIGKRLIEAGKSPSADIVADPDSDLKPLLKA
EEECCCCCCCHHHHHHHHHCCCCCCCCEEECCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]