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The map label for this gene is yngF [H]

Identifier: 89052897

GI number: 89052897

Start: 409006

End: 409617

Strand: Direct

Name: yngF [H]

Synonym: Jann_0406

Alternate gene names: 89052897

Gene position: 409006-409617 (Clockwise)

Preceding gene: 89052896

Following gene: 89052898

Centisome position: 9.47

GC content: 64.22

Gene sequence:

>612_bases
GTGATCGACGTAAGCGACCAAGATGGCCTGCGCAGTGTCACGCTCAGTCGCCCGGAGAAAGCCAATTCCCTGACCACAGC
CATGCTGACTGACCTTGTGGAGGTGTTCGAGGGTAGCCACCGCGTCCGCGCGCTGATTTTGACAGGCGCCGGGGCCAAGG
TCTTTTCAGCAGGCGCGGATCTGGAAGAGGCCCACGGGGGCCTCGCCACCTCTCCACTTTGGGAGCAAGCCTCCGGTGCG
CTTGCGGCCTTGCCGTGTCTGACCATCGCGGCGCTGAATGGCACCCTCGCTGGCGGCGCGTTCGGCATGGCGCTGGCCTG
TGACATCCGCCTCTCGGTGCCGCACGCCAAATTCTTCTACCCCGTCGCCAAACTGGGCTTCCTCCCCCAACCATCGGACC
CCGCGCGTCTGTCCGCGTTGATCGGCCCGGCCCGCACGAAACTGATCCTTTTGGCGGCCGAGAAACTCAGCGCGGATGAG
GCGCTTTCCTTCGGCCTCGTCGACCGTATCTGCACGCCGGACGATCTTCTGCCCGTCGCCCGTGACCTCGCCAGCGCCTC
TCTCAATGGCGACCCATTCACGCTTCATAAGATCAAAGCCATGATCCCCTGA

Upstream 100 bases:

>100_bases
GCAGATCCCTATCTCGGTGGCGATATCTCGCTTCGGGATGAAGGTATTCGCGCGGTGGTCGGTCTCGCATGAGCGAGAGC
GCAGATAAGGGACGCCGTTC

Downstream 100 bases:

>100_bases
CGCGCCGCTCCTCTTTCATCTGGGCCTTACAACTCCGGGGGTTTGGGGGCAGCGCCCCCGATTATCGAACACATGACGCA
ACACCACATCGACGCGCTCG

Product: enoyl-CoA hydratase/isomerase

Products: crotonoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MIDVSDQDGLRSVTLSRPEKANSLTTAMLTDLVEVFEGSHRVRALILTGAGAKVFSAGADLEEAHGGLATSPLWEQASGA
LAALPCLTIAALNGTLAGGAFGMALACDIRLSVPHAKFFYPVAKLGFLPQPSDPARLSALIGPARTKLILLAAEKLSADE
ALSFGLVDRICTPDDLLPVARDLASASLNGDPFTLHKIKAMIP

Sequences:

>Translated_203_residues
MIDVSDQDGLRSVTLSRPEKANSLTTAMLTDLVEVFEGSHRVRALILTGAGAKVFSAGADLEEAHGGLATSPLWEQASGA
LAALPCLTIAALNGTLAGGAFGMALACDIRLSVPHAKFFYPVAKLGFLPQPSDPARLSALIGPARTKLILLAAEKLSADE
ALSFGLVDRICTPDDLLPVARDLASASLNGDPFTLHKIKAMIP
>Mature_203_residues
MIDVSDQDGLRSVTLSRPEKANSLTTAMLTDLVEVFEGSHRVRALILTGAGAKVFSAGADLEEAHGGLATSPLWEQASGA
LAALPCLTIAALNGTLAGGAFGMALACDIRLSVPHAKFFYPVAKLGFLPQPSDPARLSALIGPARTKLILLAAEKLSADE
ALSFGLVDRICTPDDLLPVARDLASASLNGDPFTLHKIKAMIP

Specific function: Catalyzes The Reversible Dehydration Of L-Carnitinyl-CoA To Crotobetainyl-CoA. [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI157694516, Length=214, Percent_Identity=26.1682242990654, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI213417737, Length=214, Percent_Identity=26.1682242990654, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI194097323, Length=178, Percent_Identity=30.3370786516854, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI70995211, Length=186, Percent_Identity=29.5698924731183, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI157694520, Length=184, Percent_Identity=25.5434782608696, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI221142681, Length=199, Percent_Identity=34.6733668341709, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787659, Length=206, Percent_Identity=33.0097087378641, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790281, Length=221, Percent_Identity=30.7692307692308, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788682, Length=190, Percent_Identity=29.4736842105263, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787660, Length=208, Percent_Identity=31.25, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=188, Percent_Identity=28.1914893617021, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554946, Length=209, Percent_Identity=25.8373205741627, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=176, Percent_Identity=28.4090909090909, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI20129971, Length=181, Percent_Identity=29.2817679558011, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24653477, Length=181, Percent_Identity=29.2817679558011, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24653139, Length=177, Percent_Identity=28.8135593220339, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.55

Molecular weight: Translated: 21114; Mature: 21114

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVSDQDGLRSVTLSRPEKANSLTTAMLTDLVEVFEGSHRVRALILTGAGAKVFSAGAD
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEECCCC
LEEAHGGLATSPLWEQASGALAALPCLTIAALNGTLAGGAFGMALACDIRLSVPHAKFFY
HHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCHHCEEEEEEEEEECCCHHHHH
PVAKLGFLPQPSDPARLSALIGPARTKLILLAAEKLSADEALSFGLVDRICTPDDLLPVA
HHHHHCCCCCCCCHHHHHHHHCCCHHEEEEEEHHHCCHHHHHHHHHHHHCCCCHHHHHHH
RDLASASLNGDPFTLHKIKAMIP
HHHHHCCCCCCCEEHHHHEECCC
>Mature Secondary Structure
MIDVSDQDGLRSVTLSRPEKANSLTTAMLTDLVEVFEGSHRVRALILTGAGAKVFSAGAD
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEECCCC
LEEAHGGLATSPLWEQASGALAALPCLTIAALNGTLAGGAFGMALACDIRLSVPHAKFFY
HHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCCHHCEEEEEEEEEECCCHHHHH
PVAKLGFLPQPSDPARLSALIGPARTKLILLAAEKLSADEALSFGLVDRICTPDDLLPVA
HHHHHCCCCCCCCHHHHHHHHCCCHHEEEEEEHHHCCHHHHHHHHHHHHCCCCHHHHHHH
RDLASASLNGDPFTLHKIKAMIP
HHHHHCCCCCCCEEHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxybutanoyl-CoA

Specific reaction: (3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]