Click here to switch to the map view.

The map label for this gene is waaA [H]

Identifier: 89052891

GI number: 89052891

Start: 401998

End: 403290

Strand: Reverse

Name: waaA [H]

Synonym: Jann_0400

Alternate gene names: 89052891

Gene position: 403290-401998 (Counterclockwise)

Preceding gene: 89052892

Following gene: 89052890

Centisome position: 9.34

GC content: 64.04

Gene sequence:

>1293_bases
ATGAACCGCTCCTTTGTCCTTGGCCTCTATCTGGCGTGGTCCGCGCGTGGTGCACGGGCTTTTGCCGAGCGCAAGCTGAG
CCAGCGTCTTGCGGCTGGGAAGGAAGATGGTGCGCGTCTGGATGAGCGTCGTGGTATTGCCAATATGCCACGCCCTGACG
GCCCCCTGATCTGGTTTCATGCCGCCTCGGTCGGGGAATCGCTCGCGGTGCTGGAACTGATCCGCCGCCTGTTGGATGAA
CGCGATGATCTACATCTGCTGGTGACCACCGGCACCGTGACCTCTGCCGCCGTCATGGCCGAGCGTCTGCCGGACCGCGC
GATCCACCATTATGCACCGCTGGATGCCAAGCCGTTCGTCACCGCCTTCCTTGACCATTGGCAGCCCGATGTGGCGATCT
GGACGGAGAGTGAGCTGTGGCCCACCCTGATTGTGGAAACCCACGCCCGCGATATTCCCATGCTGCTCCTGAACGCGCGG
ATGTCGAAATCCAGCCATGACAAGTGGCGGTTTGCGCGCGGCATGGCCCAAAGCCTGCTGGAACGGTTCCAGACCGCGCT
GGTCCAGGACAATCTGACGATGGTCTATCTGCGGCGCCTGGGCATGCCCGTCAGCCGGATGAAGGTCATGGGCACCCTGA
AGGAAGGCGCCGCCGCGCTGCCCTGCAATGAAGATGATCGCGCGGCCATGGCGGCGGATCTTGCCGGGCGGCCCGTCTGG
CTGGCGGCCTCCACCCATGAGGGGGAGGAAAAGATGGTGCTGCAAGCCCACCGTATGGCGATGCGCTCCTCTCCCCGACT
GCTGCTGATCCTTGTGCCCAGGCATCCCCATCGCGGGGATGAGATTGCGGACCATATGCGGACGGAGGGGTGGCGGTTCA
CCCGGCGCTCTGCCGATGAAGACCCCGCCGATGAGGCACCTGTTTACCTGGCGGACACGATGGGTGAGATGGGCCTTTGG
TATCGCCTCTCGCCGATCAGCTTCGTGGGCGGCAGCCTTGTCGCCATCGGCGGGCACAACCCGTTTGAACCGGCGGCCCT
TGGATCCGCTATTTTGCACGGGCCCTATGTCACCAACTTCGTCGACATCTATGACCGCCTGCGCGATGGCGGTGCTGCGC
AACTGGTGTCGTCACCGGAGAAGCTGGCCGGGCAAGTGGCGGAGCTTCTCAACCCCGATGAGGCCGCGAATATGGCGGCA
GCGGCGTGGCAGGTGATCTCGGACGGTGCTGACGTGACGGATCGCGCCCTGGCGCTGATCATCGACACGTTGGAAGAGGC
AGAGACACCCTGA

Upstream 100 bases:

>100_bases
GGACAATGCCCATATGCGCTACTTCATTGCCCATATTCACCGCCTGCGTGATGAGGGGGTCGAGGCGTCCCCGACCGAAG
AACTGGGCAGCTAAACGCCG

Downstream 100 bases:

>100_bases
TGCGCGCGCCGGGGTTCTGGCATGAACCGGCGGGCGTGGCCTCGACCTTGCTGGCCCCCCTTGGCGCGCTTTACGCGGCT
GGCACAGCGCGACGCTTGCG

Product: three-deoxy-D-manno-octulosonic-acid transferase-like protein

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNRSFVLGLYLAWSARGARAFAERKLSQRLAAGKEDGARLDERRGIANMPRPDGPLIWFHAASVGESLAVLELIRRLLDE
RDDLHLLVTTGTVTSAAVMAERLPDRAIHHYAPLDAKPFVTAFLDHWQPDVAIWTESELWPTLIVETHARDIPMLLLNAR
MSKSSHDKWRFARGMAQSLLERFQTALVQDNLTMVYLRRLGMPVSRMKVMGTLKEGAAALPCNEDDRAAMAADLAGRPVW
LAASTHEGEEKMVLQAHRMAMRSSPRLLLILVPRHPHRGDEIADHMRTEGWRFTRRSADEDPADEAPVYLADTMGEMGLW
YRLSPISFVGGSLVAIGGHNPFEPAALGSAILHGPYVTNFVDIYDRLRDGGAAQLVSSPEKLAGQVAELLNPDEAANMAA
AAWQVISDGADVTDRALALIIDTLEEAETP

Sequences:

>Translated_430_residues
MNRSFVLGLYLAWSARGARAFAERKLSQRLAAGKEDGARLDERRGIANMPRPDGPLIWFHAASVGESLAVLELIRRLLDE
RDDLHLLVTTGTVTSAAVMAERLPDRAIHHYAPLDAKPFVTAFLDHWQPDVAIWTESELWPTLIVETHARDIPMLLLNAR
MSKSSHDKWRFARGMAQSLLERFQTALVQDNLTMVYLRRLGMPVSRMKVMGTLKEGAAALPCNEDDRAAMAADLAGRPVW
LAASTHEGEEKMVLQAHRMAMRSSPRLLLILVPRHPHRGDEIADHMRTEGWRFTRRSADEDPADEAPVYLADTMGEMGLW
YRLSPISFVGGSLVAIGGHNPFEPAALGSAILHGPYVTNFVDIYDRLRDGGAAQLVSSPEKLAGQVAELLNPDEAANMAA
AAWQVISDGADVTDRALALIIDTLEEAETP
>Mature_430_residues
MNRSFVLGLYLAWSARGARAFAERKLSQRLAAGKEDGARLDERRGIANMPRPDGPLIWFHAASVGESLAVLELIRRLLDE
RDDLHLLVTTGTVTSAAVMAERLPDRAIHHYAPLDAKPFVTAFLDHWQPDVAIWTESELWPTLIVETHARDIPMLLLNAR
MSKSSHDKWRFARGMAQSLLERFQTALVQDNLTMVYLRRLGMPVSRMKVMGTLKEGAAALPCNEDDRAAMAADLAGRPVW
LAASTHEGEEKMVLQAHRMAMRSSPRLLLILVPRHPHRGDEIADHMRTEGWRFTRRSADEDPADEAPVYLADTMGEMGLW
YRLSPISFVGGSLVAIGGHNPFEPAALGSAILHGPYVTNFVDIYDRLRDGGAAQLVSSPEKLAGQVAELLNPDEAANMAA
AAWQVISDGADVTDRALALIIDTLEEAETP

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers a single molecule of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=413, Percent_Identity=35.3510895883777, Blast_Score=210, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 47497; Mature: 47497

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSFVLGLYLAWSARGARAFAERKLSQRLAAGKEDGARLDERRGIANMPRPDGPLIWFH
CCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEEEE
AASVGESLAVLELIRRLLDERDDLHLLVTTGTVTSAAVMAERLPDRAIHHYAPLDAKPFV
CCHHCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCHHHHHHCCCCCCCHHH
TAFLDHWQPDVAIWTESELWPTLIVETHARDIPMLLLNARMSKSSHDKWRFARGMAQSLL
HHHHHCCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
ERFQTALVQDNLTMVYLRRLGMPVSRMKVMGTLKEGAAALPCNEDDRAAMAADLAGRPVW
HHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCEECCCCCCCHHHHHHHCCCCCEE
LAASTHEGEEKMVLQAHRMAMRSSPRLLLILVPRHPHRGDEIADHMRTEGWRFTRRSADE
EEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCEECCCCCCC
DPADEAPVYLADTMGEMGLWYRLSPISFVGGSLVAIGGHNPFEPAALGSAILHGPYVTNF
CCCCCCCEEEECCCCCCCCEEEECCHHHCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHH
VDIYDRLRDGGAAQLVSSPEKLAGQVAELLNPDEAANMAAAAWQVISDGADVTDRALALI
HHHHHHHCCCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IDTLEEAETP
HHHHHHCCCC
>Mature Secondary Structure
MNRSFVLGLYLAWSARGARAFAERKLSQRLAAGKEDGARLDERRGIANMPRPDGPLIWFH
CCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEEEE
AASVGESLAVLELIRRLLDERDDLHLLVTTGTVTSAAVMAERLPDRAIHHYAPLDAKPFV
CCHHCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCHHHHHHCCCCCCCHHH
TAFLDHWQPDVAIWTESELWPTLIVETHARDIPMLLLNARMSKSSHDKWRFARGMAQSLL
HHHHHCCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
ERFQTALVQDNLTMVYLRRLGMPVSRMKVMGTLKEGAAALPCNEDDRAAMAADLAGRPVW
HHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHCCCEECCCCCCCHHHHHHHCCCCCEE
LAASTHEGEEKMVLQAHRMAMRSSPRLLLILVPRHPHRGDEIADHMRTEGWRFTRRSADE
EEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCEECCCCCCC
DPADEAPVYLADTMGEMGLWYRLSPISFVGGSLVAIGGHNPFEPAALGSAILHGPYVTNF
CCCCCCCEEEECCCCCCCCEEEECCHHHCCCCEEEECCCCCCCHHHHHHHHHCCCHHHHH
VDIYDRLRDGGAAQLVSSPEKLAGQVAELLNPDEAANMAAAAWQVISDGADVTDRALALI
HHHHHHHCCCCHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IDTLEEAETP
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952982; 7542800 [H]