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The map label for this gene is mltB [H]

Identifier: 89052862

GI number: 89052862

Start: 373855

End: 375027

Strand: Direct

Name: mltB [H]

Synonym: Jann_0371

Alternate gene names: 89052862

Gene position: 373855-375027 (Clockwise)

Preceding gene: 89052861

Following gene: 89052863

Centisome position: 8.66

GC content: 65.81

Gene sequence:

>1173_bases
ATGCGTTTTCTCGCCCCGCTTCTTTTGTCTCTTTCCATCGGCACGGCCGCCCATGCGCAGCAATGCGGCGGCGATTTCAA
CCAGTTTGTTCAGGGCCTGCGGGCGGAAGCCGCAGAGCGGGGCCATAGTCAGGAGGCTATCGACGGCTTCTTCCGCCACG
CCAGCTATTATCAGCGCGCTCTGGATGCGGACCGGCGTCAGGGCATCTTCACCGTGCCCTTCACCGATTTCGCCCGCCGT
CTGATCAGTCAGGGCCGCATGGACAATGGACGGCGCAACGCCGATCGTCAGGCCGCCACCTTCGCCGAGATTGAGCGGCG
TTTCGGCGTATCCCAGGGCATTCTTCTGGCCTTCTGGGCGTTCGAGACGGATTACGGCGCGTTTCAGGGCGATTACAACA
CGCTCGACAGCCTCGTGACCCTCGCCCACGATTGCCGCCGGCCCGAGCTGTTCCGCCCCGAAGTGTTCGGCGCTCTGGAA
TTGTACGAGATGGGCGATTTCGACCCGGTGAACACCCAAGGCGCCTGGGCCGGGGAAATCGGCATGGTCCAGATGCTGCC
CGAAGATATCGTCGCCCACGGCATTGACGGCGACGGCGACGGCCACGTCTACCTGCAAACCTCCGCGCCCGATGCGCTGA
TGTCGGGTGCCAACATCCTCTCGTCCCTCGGCTGGCGTCCCGGTGAGCCCTGGCTCGTCGAAATCACCGTTCCGCAGGAT
CTGGACTGGTATCAGACCGGCATCCACACCACCCGCTCCGTCGCCGACTGGCAGGCCGATGGTGTGGCGGGTCGCTCGGG
CGCCCTGCCGATGCCAAACGCCCAGGCCTCGATCGTCCTGCCCCAAGGCCGCAATGGCCCCGCCTTCATGGCCTACCAGA
ACTTCAACGTCCTCTTTGAATGGAACCAGTCCTTCACCTACGTCCTGACCGCCGCCTATTTCGCAACCCGTCTGGAAGGT
GCGCCGGTGTTTGACGCGCGCAACCCGGAACCGGGCCTGGGCGGGGACCAGATGCGCCGCCTCCAGACCGCCCTCCAGGC
GCGGGGCTATGACGTGGGGCGCATCGACGGCATCCTCGGCGCTGGCACCCGGGCCGCCGTGCGGGAAGAACAGCGCCGCC
TGGGCCTGCCCGCCGATGCCTGGCCCACCAGCGCCCTGTTAAACGCGCTGTAA

Upstream 100 bases:

>100_bases
GCTTCTTGCCACGGTTTTGCTTGCGCTCATCTGAACGGTTGCGAGCCGCGCCAACCACGCTAATCTCCCCCAAATCCATA
AGTTATGAGGTTGTCCCCCC

Downstream 100 bases:

>100_bases
TCTTCAATACCGCGCAATTTGTCCGAAAACCGGTTCCCACTTTTCGGATCGCGCTCTGAATTAACCAGACGCTAACCAAA
CCACCCGATCCTGCTTGCCC

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MRFLAPLLLSLSIGTAAHAQQCGGDFNQFVQGLRAEAAERGHSQEAIDGFFRHASYYQRALDADRRQGIFTVPFTDFARR
LISQGRMDNGRRNADRQAATFAEIERRFGVSQGILLAFWAFETDYGAFQGDYNTLDSLVTLAHDCRRPELFRPEVFGALE
LYEMGDFDPVNTQGAWAGEIGMVQMLPEDIVAHGIDGDGDGHVYLQTSAPDALMSGANILSSLGWRPGEPWLVEITVPQD
LDWYQTGIHTTRSVADWQADGVAGRSGALPMPNAQASIVLPQGRNGPAFMAYQNFNVLFEWNQSFTYVLTAAYFATRLEG
APVFDARNPEPGLGGDQMRRLQTALQARGYDVGRIDGILGAGTRAAVREEQRRLGLPADAWPTSALLNAL

Sequences:

>Translated_390_residues
MRFLAPLLLSLSIGTAAHAQQCGGDFNQFVQGLRAEAAERGHSQEAIDGFFRHASYYQRALDADRRQGIFTVPFTDFARR
LISQGRMDNGRRNADRQAATFAEIERRFGVSQGILLAFWAFETDYGAFQGDYNTLDSLVTLAHDCRRPELFRPEVFGALE
LYEMGDFDPVNTQGAWAGEIGMVQMLPEDIVAHGIDGDGDGHVYLQTSAPDALMSGANILSSLGWRPGEPWLVEITVPQD
LDWYQTGIHTTRSVADWQADGVAGRSGALPMPNAQASIVLPQGRNGPAFMAYQNFNVLFEWNQSFTYVLTAAYFATRLEG
APVFDARNPEPGLGGDQMRRLQTALQARGYDVGRIDGILGAGTRAAVREEQRRLGLPADAWPTSALLNAL
>Mature_390_residues
MRFLAPLLLSLSIGTAAHAQQCGGDFNQFVQGLRAEAAERGHSQEAIDGFFRHASYYQRALDADRRQGIFTVPFTDFARR
LISQGRMDNGRRNADRQAATFAEIERRFGVSQGILLAFWAFETDYGAFQGDYNTLDSLVTLAHDCRRPELFRPEVFGALE
LYEMGDFDPVNTQGAWAGEIGMVQMLPEDIVAHGIDGDGDGHVYLQTSAPDALMSGANILSSLGWRPGEPWLVEITVPQD
LDWYQTGIHTTRSVADWQADGVAGRSGALPMPNAQASIVLPQGRNGPAFMAYQNFNVLFEWNQSFTYVLTAAYFATRLEG
APVFDARNPEPGLGGDQMRRLQTALQARGYDVGRIDGILGAGTRAAVREEQRRLGLPADAWPTSALLNAL

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=236, Percent_Identity=26.6949152542373, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 42882; Mature: 42882

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLAPLLLSLSIGTAAHAQQCGGDFNQFVQGLRAEAAERGHSQEAIDGFFRHASYYQRA
CCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LDADRRQGIFTVPFTDFARRLISQGRMDNGRRNADRQAATFAEIERRFGVSQGILLAFWA
HHHHHHCCEEEECHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
FETDYGAFQGDYNTLDSLVTLAHDCRRPELFRPEVFGALELYEMGDFDPVNTQGAWAGEI
HCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
GMVQMLPEDIVAHGIDGDGDGHVYLQTSAPDALMSGANILSSLGWRPGEPWLVEITVPQD
CHHHHCHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
LDWYQTGIHTTRSVADWQADGVAGRSGALPMPNAQASIVLPQGRNGPAFMAYQNFNVLFE
CCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCEEEEE
WNQSFTYVLTAAYFATRLEGAPVFDARNPEPGLGGDQMRRLQTALQARGYDVGRIDGILG
ECCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCHHC
AGTRAAVREEQRRLGLPADAWPTSALLNAL
CCCHHHHHHHHHHCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MRFLAPLLLSLSIGTAAHAQQCGGDFNQFVQGLRAEAAERGHSQEAIDGFFRHASYYQRA
CCHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LDADRRQGIFTVPFTDFARRLISQGRMDNGRRNADRQAATFAEIERRFGVSQGILLAFWA
HHHHHHCCEEEECHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEE
FETDYGAFQGDYNTLDSLVTLAHDCRRPELFRPEVFGALELYEMGDFDPVNTQGAWAGEI
HCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
GMVQMLPEDIVAHGIDGDGDGHVYLQTSAPDALMSGANILSSLGWRPGEPWLVEITVPQD
CHHHHCHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
LDWYQTGIHTTRSVADWQADGVAGRSGALPMPNAQASIVLPQGRNGPAFMAYQNFNVLFE
CCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCEEEEE
WNQSFTYVLTAAYFATRLEGAPVFDARNPEPGLGGDQMRRLQTALQARGYDVGRIDGILG
ECCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCHHC
AGTRAAVREEQRRLGLPADAWPTSALLNAL
CCCHHHHHHHHHHCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]