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The map label for this gene is cobD

Identifier: 89052861

GI number: 89052861

Start: 372829

End: 373788

Strand: Direct

Name: cobD

Synonym: Jann_0370

Alternate gene names: 89052861

Gene position: 372829-373788 (Clockwise)

Preceding gene: 89052860

Following gene: 89052862

Centisome position: 8.63

GC content: 66.25

Gene sequence:

>960_bases
TTGATCGCCACCGCTGGGTTGCTGTTTGCTGCGATGATCCTGGATGCGCTGGTGGGCGAGCCCCGGTGGCTGTGGTCCCG
GATCCCGCATCCGATCGTGCTGATGGGGCGGTTGATCGACTGGTGCGATGCCACGTTCAATCGCGGGGCGCAGCGGCTCA
ACGGGGGTCTGGTCATGGCAGCACTTGGCCTGAGCGCATTGGGGTTGGGTTGGGTCATTGCCGCCCTGCCGCTCGGGTGG
CTGTGGCAAATCCTGATCGCCGCGGTTTTGCTGGCGCAGAGGTCTTTGGTGGACCATGTGGCGGCGGTCGCTGTCGGTCT
CCGCCGGTCGCTGGCGGACGGCAAGCGCGCCGTTGCGATGATCGTCGGGCGCGACACCGGGCCATTGGATGACGCCGCCG
TGGCCCGCGCCGCGATTGAGAGTGCGGCAGAGAACATGTCCGATGGTGTCGTCGCGCCCGCATTCTGGTTTCTGGTCGGC
GGGCTGCCCGGGTTATTGTTGTATAAGATCACGAACACGGCGGATTCGATGATCGGCCATCGCACGCCCCGGCACGAGGC
CTTCGGATGGGCGGCGGCGCGGTTCGATGACGTGTTGAACTATGTCCCCGCGCGGCTGACGGCCTTGTTGATCGCACTCA
CCCACGGGTTTGACGCGCTGCGCTGTGCCTATCGCGACGGTCCCAAACATCGGTCGGTGAATGCGGGCTGGCCGGAGGCG
GCGATGGCCTCCGCCCTCGGTCTGTCCTTGTCGGGCTCGCGCGCCTATGGCGGGGAGATGACAGAGGATCCGGCCCTGAA
CGCGACCGGGTCACGCGATGCCACCCCCGGCGATATAAAAGCGGCGATCCGCGTTCTGTGGCGCGTCTGGTGGGCGATGC
TTGGCGGCAGCGTCATCTGCATCCTTCTCGGCGGGTGGGGGGCGCTGCTTCTTGCCACGGTTTTGCTTGCGCTCATCTGA

Upstream 100 bases:

>100_bases
AGCATCGCATCTGGTCACGCATTTTCCCCTATTCCACCAATTGGATGCGCCTGGGTCTTCCCGCCACGGATGAGGATTGG
GCGCGCCTGGACGCGGCCCT

Downstream 100 bases:

>100_bases
ACGGTTGCGAGCCGCGCCAACCACGCTAATCTCCCCCAAATCCATAAGTTATGAGGTTGTCCCCCCATGCGTTTTCTCGC
CCCGCTTCTTTTGTCTCTTT

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MIATAGLLFAAMILDALVGEPRWLWSRIPHPIVLMGRLIDWCDATFNRGAQRLNGGLVMAALGLSALGLGWVIAALPLGW
LWQILIAAVLLAQRSLVDHVAAVAVGLRRSLADGKRAVAMIVGRDTGPLDDAAVARAAIESAAENMSDGVVAPAFWFLVG
GLPGLLLYKITNTADSMIGHRTPRHEAFGWAAARFDDVLNYVPARLTALLIALTHGFDALRCAYRDGPKHRSVNAGWPEA
AMASALGLSLSGSRAYGGEMTEDPALNATGSRDATPGDIKAAIRVLWRVWWAMLGGSVICILLGGWGALLLATVLLALI

Sequences:

>Translated_319_residues
MIATAGLLFAAMILDALVGEPRWLWSRIPHPIVLMGRLIDWCDATFNRGAQRLNGGLVMAALGLSALGLGWVIAALPLGW
LWQILIAAVLLAQRSLVDHVAAVAVGLRRSLADGKRAVAMIVGRDTGPLDDAAVARAAIESAAENMSDGVVAPAFWFLVG
GLPGLLLYKITNTADSMIGHRTPRHEAFGWAAARFDDVLNYVPARLTALLIALTHGFDALRCAYRDGPKHRSVNAGWPEA
AMASALGLSLSGSRAYGGEMTEDPALNATGSRDATPGDIKAAIRVLWRVWWAMLGGSVICILLGGWGALLLATVLLALI
>Mature_319_residues
MIATAGLLFAAMILDALVGEPRWLWSRIPHPIVLMGRLIDWCDATFNRGAQRLNGGLVMAALGLSALGLGWVIAALPLGW
LWQILIAAVLLAQRSLVDHVAAVAVGLRRSLADGKRAVAMIVGRDTGPLDDAAVARAAIESAAENMSDGVVAPAFWFLVG
GLPGLLLYKITNTADSMIGHRTPRHEAFGWAAARFDDVLNYVPARLTALLIALTHGFDALRCAYRDGPKHRSVNAGWPEA
AMASALGLSLSGSRAYGGEMTEDPALNATGSRDATPGDIKAAIRVLWRVWWAMLGGSVICILLGGWGALLLATVLLALI

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 33752; Mature: 33752

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATAGLLFAAMILDALVGEPRWLWSRIPHPIVLMGRLIDWCDATFNRGAQRLNGGLVMA
CCCHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ALGLSALGLGWVIAALPLGWLWQILIAAVLLAQRSLVDHVAAVAVGLRRSLADGKRAVAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
IVGRDTGPLDDAAVARAAIESAAENMSDGVVAPAFWFLVGGLPGLLLYKITNTADSMIGH
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
RTPRHEAFGWAAARFDDVLNYVPARLTALLIALTHGFDALRCAYRDGPKHRSVNAGWPEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCHH
AMASALGLSLSGSRAYGGEMTEDPALNATGSRDATPGDIKAAIRVLWRVWWAMLGGSVIC
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHEE
ILLGGWGALLLATVLLALI
HHHCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MIATAGLLFAAMILDALVGEPRWLWSRIPHPIVLMGRLIDWCDATFNRGAQRLNGGLVMA
CCCHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ALGLSALGLGWVIAALPLGWLWQILIAAVLLAQRSLVDHVAAVAVGLRRSLADGKRAVAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
IVGRDTGPLDDAAVARAAIESAAENMSDGVVAPAFWFLVGGLPGLLLYKITNTADSMIGH
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
RTPRHEAFGWAAARFDDVLNYVPARLTALLIALTHGFDALRCAYRDGPKHRSVNAGWPEA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCHH
AMASALGLSLSGSRAYGGEMTEDPALNATGSRDATPGDIKAAIRVLWRVWWAMLGGSVIC
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHEE
ILLGGWGALLLATVLLALI
HHHCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA