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The map label for this gene is mltB [H]

Identifier: 89052848

GI number: 89052848

Start: 361969

End: 363513

Strand: Direct

Name: mltB [H]

Synonym: Jann_0357

Alternate gene names: 89052848

Gene position: 361969-363513 (Clockwise)

Preceding gene: 89052847

Following gene: 89052852

Centisome position: 8.38

GC content: 66.6

Gene sequence:

>1545_bases
ATGCCCGGTCTGAAATACCTGTCTTTTGCTGTGGTCACCGCCCTTGCCATGCCCGCGCTGGCGGATCCGCCGGCGGTGTC
GCCGCTTCCTATGCCGCGCCCTGCGGACGGGGCGGTGACGGTGCTGGACGTCTCGGCGCCATCGCGTCGCAACGTCCTGA
TCGAGGTGATCGCGGAGGAGGCGATTGAAGTGGCCTCAGCCGATCTGGGCCTGTCCCTCACCGAGGACGAGGTGCTGGCC
GACGAGGCTACAGCGGAGGGGGGCACAGCGGCGGAGGTGGAGATCAGTGCGCTGGCCTCCGAGACCTCTCCGCGCCCGGT
GCCCCGTGCCGACGTGCCCGCGGATTTTGCCGAACGTGCCGCGCTTTTGCGTGCGGAGGCGGAAGCGGACGAGGCGGCCC
GTCGCCCCCCACGCACCAGCGACCCGGAATTTCGCGCCTGGATCGCGGAGTTTGAAGCCCGCGCCCTGGCCGCCGGGATC
TCGGCCGAGACATTTGCCCGTGCCTTCGACGGGTTGCAGCTCAATACCCGCGTGCTGGAGCGGGACCGCAACCAGGCCGA
ATTCTCCCGCGCGCTGTGGGATTATCTTGATACCGCCGTCTCGGCCACGCGCATCCGCAACGGGCGTGAGGCGCTGTCGG
AATGGCGCACGACGCTGGGCCGGATTGAAGCGCAATATCAGGTGGAGGCAGAGGTCGTTGTCGCCGTCTGGGGCCTGGAA
AGCGCTTACGGTGCGATGCGCGGCAGCGAAGGGATCGTGGAGGCGATGGCCTCGCTTGCCTTTGATGGGCGGCGACAGGA
TTTCTTTGAAGAGCAGTTGATCGCCGCGTTGCAGATCCTGGAGGCTGGCGATACCCGGGCGCGCAATATGACCGGGTCCT
GGGCCGGGGCGATGGGGCACACGCAGTTCATGCCCACCAGTTATCTGGAATTTGCGCAGGATTTCGACGACGATGGCCGT
CGCAATATCTGGAGCGATAATCCCGTCGATGCGCTGGCTTCCACCGCCCATTATCTGGTGGAACACGGGTGGGTCTATGG
CCAGCCCTGGGGCATGGAAGTGCGCCTGCCCGAGGGGTTCGATTACCGCCTCGCCGGGGAGCAGGAGCGGATTGGCGCCG
CGTTCTGGAATGCGATGGGGGTGCGGCTGGTGAATGGCGATCCGTTGCCCGACCATGGGCCTGCGTCGATCCTGCTGCCT
GCGGGCGCGGAGGGTGTCGCGCTGGTGGTTCACAGCAATTTCCGGGTGATCGAGCGGTATAATCCGGCGGATGCCTACGT
GATTGCCATCGGACATCTGAGTGACCGGATCAATGGCGGCCCGGGGTTCGAGGCGGGATGGCCGCGCGGGGACCGGGCGC
TGACCTTTGCCGAGCGCGAGGAGTTGCAGCGCCTGTTGCGGGCCGTGGGGCATTACGATGAGCGGATCGACGGGATCGTG
GGACCGATCACGATTGCGTCCGTGCGCAGCTACCAGGAGGCGATTGGCGTGACGCCGGACGGCTACGCCTCGGTCTCGAT
CCTGCAACGGTTGCGGGACGGGTGA

Upstream 100 bases:

>100_bases
GGGTGACCGGCAAAAGCCCGCCCAACATTTGCGGAAATGTGTTAGTCTTGCGCAATTCCGCTATAGTGTCGCGCTGTTGA
GGATCATCGAACCTGTGCCC

Downstream 100 bases:

>100_bases
CAGGGATTGCGCCGCTCGCGCGTCGCCCGGCCCCTCCTCCGCACGATGTGCGTCCTCGGGGCTATGCCGGGGTGACCGCC
GCGCCGATGGTCAGCAGGCC

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MPGLKYLSFAVVTALAMPALADPPAVSPLPMPRPADGAVTVLDVSAPSRRNVLIEVIAEEAIEVASADLGLSLTEDEVLA
DEATAEGGTAAEVEISALASETSPRPVPRADVPADFAERAALLRAEAEADEAARRPPRTSDPEFRAWIAEFEARALAAGI
SAETFARAFDGLQLNTRVLERDRNQAEFSRALWDYLDTAVSATRIRNGREALSEWRTTLGRIEAQYQVEAEVVVAVWGLE
SAYGAMRGSEGIVEAMASLAFDGRRQDFFEEQLIAALQILEAGDTRARNMTGSWAGAMGHTQFMPTSYLEFAQDFDDDGR
RNIWSDNPVDALASTAHYLVEHGWVYGQPWGMEVRLPEGFDYRLAGEQERIGAAFWNAMGVRLVNGDPLPDHGPASILLP
AGAEGVALVVHSNFRVIERYNPADAYVIAIGHLSDRINGGPGFEAGWPRGDRALTFAEREELQRLLRAVGHYDERIDGIV
GPITIASVRSYQEAIGVTPDGYASVSILQRLRDG

Sequences:

>Translated_514_residues
MPGLKYLSFAVVTALAMPALADPPAVSPLPMPRPADGAVTVLDVSAPSRRNVLIEVIAEEAIEVASADLGLSLTEDEVLA
DEATAEGGTAAEVEISALASETSPRPVPRADVPADFAERAALLRAEAEADEAARRPPRTSDPEFRAWIAEFEARALAAGI
SAETFARAFDGLQLNTRVLERDRNQAEFSRALWDYLDTAVSATRIRNGREALSEWRTTLGRIEAQYQVEAEVVVAVWGLE
SAYGAMRGSEGIVEAMASLAFDGRRQDFFEEQLIAALQILEAGDTRARNMTGSWAGAMGHTQFMPTSYLEFAQDFDDDGR
RNIWSDNPVDALASTAHYLVEHGWVYGQPWGMEVRLPEGFDYRLAGEQERIGAAFWNAMGVRLVNGDPLPDHGPASILLP
AGAEGVALVVHSNFRVIERYNPADAYVIAIGHLSDRINGGPGFEAGWPRGDRALTFAEREELQRLLRAVGHYDERIDGIV
GPITIASVRSYQEAIGVTPDGYASVSILQRLRDG
>Mature_513_residues
PGLKYLSFAVVTALAMPALADPPAVSPLPMPRPADGAVTVLDVSAPSRRNVLIEVIAEEAIEVASADLGLSLTEDEVLAD
EATAEGGTAAEVEISALASETSPRPVPRADVPADFAERAALLRAEAEADEAARRPPRTSDPEFRAWIAEFEARALAAGIS
AETFARAFDGLQLNTRVLERDRNQAEFSRALWDYLDTAVSATRIRNGREALSEWRTTLGRIEAQYQVEAEVVVAVWGLES
AYGAMRGSEGIVEAMASLAFDGRRQDFFEEQLIAALQILEAGDTRARNMTGSWAGAMGHTQFMPTSYLEFAQDFDDDGRR
NIWSDNPVDALASTAHYLVEHGWVYGQPWGMEVRLPEGFDYRLAGEQERIGAAFWNAMGVRLVNGDPLPDHGPASILLPA
GAEGVALVVHSNFRVIERYNPADAYVIAIGHLSDRINGGPGFEAGWPRGDRALTFAEREELQRLLRAVGHYDERIDGIVG
PITIASVRSYQEAIGVTPDGYASVSILQRLRDG

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=250, Percent_Identity=32.8, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 55803; Mature: 55672

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGLKYLSFAVVTALAMPALADPPAVSPLPMPRPADGAVTVLDVSAPSRRNVLIEVIAEE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHH
AIEVASADLGLSLTEDEVLADEATAEGGTAAEVEISALASETSPRPVPRADVPADFAERA
HHHHHHCCCCCEECCHHHHHHHCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCHHHHHHH
ALLRAEAEADEAARRPPRTSDPEFRAWIAEFEARALAAGISAETFARAFDGLQLNTRVLE
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHH
RDRNQAEFSRALWDYLDTAVSATRIRNGREALSEWRTTLGRIEAQYQVEAEVVVAVWGLE
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCEEEEEEEEEEEEEECHH
SAYGAMRGSEGIVEAMASLAFDGRRQDFFEEQLIAALQILEAGDTRARNMTGSWAGAMGH
HHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHCCCCC
TQFMPTSYLEFAQDFDDDGRRNIWSDNPVDALASTAHYLVEHGWVYGQPWGMEVRLPEGF
CCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCEEECCCCC
DYRLAGEQERIGAAFWNAMGVRLVNGDPLPDHGPASILLPAGAEGVALVVHSNFRVIERY
CEEECCCHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEECCCCCEEEEEECCEEEEEEC
NPADAYVIAIGHLSDRINGGPGFEAGWPRGDRALTFAEREELQRLLRAVGHYDERIDGIV
CCCCEEEEEEECHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHCCCC
GPITIASVRSYQEAIGVTPDGYASVSILQRLRDG
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PGLKYLSFAVVTALAMPALADPPAVSPLPMPRPADGAVTVLDVSAPSRRNVLIEVIAEE
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHH
AIEVASADLGLSLTEDEVLADEATAEGGTAAEVEISALASETSPRPVPRADVPADFAERA
HHHHHHCCCCCEECCHHHHHHHCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCHHHHHHH
ALLRAEAEADEAARRPPRTSDPEFRAWIAEFEARALAAGISAETFARAFDGLQLNTRVLE
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHH
RDRNQAEFSRALWDYLDTAVSATRIRNGREALSEWRTTLGRIEAQYQVEAEVVVAVWGLE
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCEEEEEEEEEEEEEECHH
SAYGAMRGSEGIVEAMASLAFDGRRQDFFEEQLIAALQILEAGDTRARNMTGSWAGAMGH
HHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHCCCCC
TQFMPTSYLEFAQDFDDDGRRNIWSDNPVDALASTAHYLVEHGWVYGQPWGMEVRLPEGF
CCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCEEECCCCC
DYRLAGEQERIGAAFWNAMGVRLVNGDPLPDHGPASILLPAGAEGVALVVHSNFRVIERY
CEEECCCHHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEECCCCCEEEEEECCEEEEEEC
NPADAYVIAIGHLSDRINGGPGFEAGWPRGDRALTFAEREELQRLLRAVGHYDERIDGIV
CCCCEEEEEEECHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHCCCC
GPITIASVRSYQEAIGVTPDGYASVSILQRLRDG
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]