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The map label for this gene is thiP

Identifier: 89052820

GI number: 89052820

Start: 334134

End: 335699

Strand: Reverse

Name: thiP

Synonym: Jann_0329

Alternate gene names: 89052820

Gene position: 335699-334134 (Counterclockwise)

Preceding gene: 89052821

Following gene: 89052819

Centisome position: 7.77

GC content: 65.64

Gene sequence:

>1566_bases
ATGGCAAAACGCGCTCAGCCAATAACGCTGGTGCAGGGGATCGGCGGGGCGTTTGTCCTGCTGGTTATCCTCGCGATCCT
CGGCCCCCTCGGCGCAGTGATGATCCGGGCTGAGGGGGTCAATGGTTTGCGCCCTGCCGATTGGGCGGCCGTCCGGTTTA
CGCTTCTTCAAGCGGTCCTGTCTGCCGTTGTGTCCTGCGTCGCGGCGATCCCCGTTGCGCGGGCCTTGGCGCGGCGGCGG
TTCCGCGGGCGCGGCGCGTATATCGCCCTGATGGGCGCGCCATTCATCCTGCCGGTCATTGTCGCCGTGATGGGCCTTCT
GGCGATCTTCGGACGCGCAGGTTGGCTGAATGCCGCACTGGAAGGGATCGGCGCGGGCACGATTGAGATTTATGGCCTCG
CGGGCGTCGTCATGGCCCACGTTTTCCTGAACCTGCCGCTCGCCACGCGGCTGATTTTGCAGGCGTGGTTGTCTGTCCCG
TCTGAGCGCATCCGCCTTGCCCATTCACTCAATTTCACCGCCCAGGACATGCGCCGCCATTTTGAGTGGCCGTTGCTGCG
GGCCACGCTGCCCGGCGCGGCGCTGATTATTTTCCTCATCTGCACCACCAGCTTTGCCGTCGCCTTGATCCTTGGCGGTG
GACCATCTGCGACAACCGTTGAACTTGCGATCTACCAGGCGTTTCAGTTCGACTTCGATCTGGGCCGTGCGGCCCTTCTG
GGCCTGATCCAGGTGGGTATCTGCGTCGGTGCGGGTGTGGTTGCCTGGCGCTTCGCCACCGCGACAGAGTTCGGCGGCGG
GCTGGACCGCCCCGCGATGCATTGGCCCGGTGATCCGCATCTGTCCCGCCTGTGGGACGGCCTCGCCTTATTCGCCGCGG
GCGCTTTCTTGCTGCTGCCGCTGGCCGCTGTCACCGGCCAAGGCGTTGGCGCAATTGCCGGCCTGCCCCTTTCCACGTGG
GAGGCCGCTTTGCGTTCGATCCTCCTCGCCCTCGCCTCCACCGCATTTGCGGTCACTCTGGCCCTGCCGATCGCGCTTTT
GATCGCGGGCAAGGCGGCGCGGGCTGGTCTTGAGGGCGTCAGCCTGCTGTCCATCGCGGTGTCTCCACTGGTGATCGGCA
CCGGGTTGTTCCTGATCGTCTTTCCAATCGCCGACCCTGTGGCCCTGTCTTTGCCGATCACGGGCCTGGTGAACGCATTG
GTGGCGCTACCTTTCCTGCTCCGCGCACTTATCCCCGCCGCGACCCAAGCCGAGGCAACGCAAGGCCGCTTGGCAGACGC
CCTTGGAATGCAGGGAACGGCCCGTCTGCGCCACGCGATCCTGCCGCGCGTGCGCCGCCCCTTGGGGTTTGGCGCGGGCC
TCGCCGCAGCGTTTTCCATGGGCGACCTTGGCGTGATCGCTCTGTTCTCCACCCAGGGGCAAGAGACCCTGCCGTTGGAG
ATGTATCGCCTGTTCGGCTCCTACCGCACCAACGATGCACAGGGGGCCGCCGTGCTGCTCATGGCGTTGTCGCTGGGGAT
GTTCTGGCTGTTTGACCGAGGGGGCCGCGTGAATGCTGCATCTTGA

Upstream 100 bases:

>100_bases
ACCAGTTGCCCGAGGGGTTCGAGACGCTCATCCAACCCGAGACCGCGTTGATTTTCACCCCTGAAGAGGCCGCCGCCATC
CGCGACGACGCATTGGCGAC

Downstream 100 bases:

>100_bases
AAACATCCGCGTGCGACAGGGCAGCTTCACGCTGTCCGCCCATCTCACCATCGCGAAAGGCGCGCGGGTTGCGCTGATGG
GCGCCTCGGGCAGCGGGAAA

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MAKRAQPITLVQGIGGAFVLLVILAILGPLGAVMIRAEGVNGLRPADWAAVRFTLLQAVLSAVVSCVAAIPVARALARRR
FRGRGAYIALMGAPFILPVIVAVMGLLAIFGRAGWLNAALEGIGAGTIEIYGLAGVVMAHVFLNLPLATRLILQAWLSVP
SERIRLAHSLNFTAQDMRRHFEWPLLRATLPGAALIIFLICTTSFAVALILGGGPSATTVELAIYQAFQFDFDLGRAALL
GLIQVGICVGAGVVAWRFATATEFGGGLDRPAMHWPGDPHLSRLWDGLALFAAGAFLLLPLAAVTGQGVGAIAGLPLSTW
EAALRSILLALASTAFAVTLALPIALLIAGKAARAGLEGVSLLSIAVSPLVIGTGLFLIVFPIADPVALSLPITGLVNAL
VALPFLLRALIPAATQAEATQGRLADALGMQGTARLRHAILPRVRRPLGFGAGLAAAFSMGDLGVIALFSTQGQETLPLE
MYRLFGSYRTNDAQGAAVLLMALSLGMFWLFDRGGRVNAAS

Sequences:

>Translated_521_residues
MAKRAQPITLVQGIGGAFVLLVILAILGPLGAVMIRAEGVNGLRPADWAAVRFTLLQAVLSAVVSCVAAIPVARALARRR
FRGRGAYIALMGAPFILPVIVAVMGLLAIFGRAGWLNAALEGIGAGTIEIYGLAGVVMAHVFLNLPLATRLILQAWLSVP
SERIRLAHSLNFTAQDMRRHFEWPLLRATLPGAALIIFLICTTSFAVALILGGGPSATTVELAIYQAFQFDFDLGRAALL
GLIQVGICVGAGVVAWRFATATEFGGGLDRPAMHWPGDPHLSRLWDGLALFAAGAFLLLPLAAVTGQGVGAIAGLPLSTW
EAALRSILLALASTAFAVTLALPIALLIAGKAARAGLEGVSLLSIAVSPLVIGTGLFLIVFPIADPVALSLPITGLVNAL
VALPFLLRALIPAATQAEATQGRLADALGMQGTARLRHAILPRVRRPLGFGAGLAAAFSMGDLGVIALFSTQGQETLPLE
MYRLFGSYRTNDAQGAAVLLMALSLGMFWLFDRGGRVNAAS
>Mature_520_residues
AKRAQPITLVQGIGGAFVLLVILAILGPLGAVMIRAEGVNGLRPADWAAVRFTLLQAVLSAVVSCVAAIPVARALARRRF
RGRGAYIALMGAPFILPVIVAVMGLLAIFGRAGWLNAALEGIGAGTIEIYGLAGVVMAHVFLNLPLATRLILQAWLSVPS
ERIRLAHSLNFTAQDMRRHFEWPLLRATLPGAALIIFLICTTSFAVALILGGGPSATTVELAIYQAFQFDFDLGRAALLG
LIQVGICVGAGVVAWRFATATEFGGGLDRPAMHWPGDPHLSRLWDGLALFAAGAFLLLPLAAVTGQGVGAIAGLPLSTWE
AALRSILLALASTAFAVTLALPIALLIAGKAARAGLEGVSLLSIAVSPLVIGTGLFLIVFPIADPVALSLPITGLVNALV
ALPFLLRALIPAATQAEATQGRLADALGMQGTARLRHAILPRVRRPLGFGAGLAAAFSMGDLGVIALFSTQGQETLPLEM
YRLFGSYRTNDAQGAAVLLMALSLGMFWLFDRGGRVNAAS

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=461, Percent_Identity=39.2624728850325, Blast_Score=266, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 54281; Mature: 54150

Theoretical pI: Translated: 11.26; Mature: 11.26

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRAQPITLVQGIGGAFVLLVILAILGPLGAVMIRAEGVNGLRPADWAAVRFTLLQAVL
CCCCCCCHHHHHHCCHHHHHHHHHHHHCCHHEEEEEECCCCCCCCCHHHHHHHHHHHHHH
SAVVSCVAAIPVARALARRRFRGRGAYIALMGAPFILPVIVAVMGLLAIFGRAGWLNAAL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHH
EGIGAGTIEIYGLAGVVMAHVFLNLPLATRLILQAWLSVPSERIRLAHSLNFTAQDMRRH
HHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHH
FEWPLLRATLPGAALIIFLICTTSFAVALILGGGPSATTVELAIYQAFQFDFDLGRAALL
CCCCHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCCCEEHHHHHHHHHCCHHHHHHHHH
GLIQVGICVGAGVVAWRFATATEFGGGLDRPAMHWPGDPHLSRLWDGLALFAAGAFLLLP
HHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LAAVTGQGVGAIAGLPLSTWEAALRSILLALASTAFAVTLALPIALLIAGKAARAGLEGV
HHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHH
SLLSIAVSPLVIGTGLFLIVFPIADPVALSLPITGLVNALVALPFLLRALIPAATQAEAT
HHHHHHHHHHHHHCCHHHEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHH
QGRLADALGMQGTARLRHAILPRVRRPLGFGAGLAAAFSMGDLGVIALFSTQGQETLPLE
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCCHHH
MYRLFGSYRTNDAQGAAVLLMALSLGMFWLFDRGGRVNAAS
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AKRAQPITLVQGIGGAFVLLVILAILGPLGAVMIRAEGVNGLRPADWAAVRFTLLQAVL
CCCCCCHHHHHHCCHHHHHHHHHHHHCCHHEEEEEECCCCCCCCCHHHHHHHHHHHHHH
SAVVSCVAAIPVARALARRRFRGRGAYIALMGAPFILPVIVAVMGLLAIFGRAGWLNAAL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHH
EGIGAGTIEIYGLAGVVMAHVFLNLPLATRLILQAWLSVPSERIRLAHSLNFTAQDMRRH
HHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHH
FEWPLLRATLPGAALIIFLICTTSFAVALILGGGPSATTVELAIYQAFQFDFDLGRAALL
CCCCHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCCCEEHHHHHHHHHCCHHHHHHHHH
GLIQVGICVGAGVVAWRFATATEFGGGLDRPAMHWPGDPHLSRLWDGLALFAAGAFLLLP
HHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LAAVTGQGVGAIAGLPLSTWEAALRSILLALASTAFAVTLALPIALLIAGKAARAGLEGV
HHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHH
SLLSIAVSPLVIGTGLFLIVFPIADPVALSLPITGLVNALVALPFLLRALIPAATQAEAT
HHHHHHHHHHHHHCCHHHEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHH
QGRLADALGMQGTARLRHAILPRVRRPLGFGAGLAAAFSMGDLGVIALFSTQGQETLPLE
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCCHHH
MYRLFGSYRTNDAQGAAVLLMALSLGMFWLFDRGGRVNAAS
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]