Click here to switch to the map view.

The map label for this gene is tbpA

Identifier: 89052821

GI number: 89052821

Start: 335675

End: 336682

Strand: Reverse

Name: tbpA

Synonym: Jann_0330

Alternate gene names: 89052821

Gene position: 336682-335675 (Counterclockwise)

Preceding gene: 89052824

Following gene: 89052820

Centisome position: 7.8

GC content: 62.4

Gene sequence:

>1008_bases
ATGAAACTCACCAACACGACGATCCTTACGGCGGGCATCACTGCGGGTCTGACCCTTTTGACCGGGGCCGCCTTCGCGCA
AGTCCCTGTCTTGACGGTCTACACCTATGACAGCTTCAATTCCGAGTGGGGCCCCGGCCCCGGCGTCGAATTGGCTTTCG
AAGAGATCTGCGAGTGCGACCTGCAATTCACCAGCACGGGCGACGGCGCGGCTTTGCTGGCGCGGTTGCGTCTGGAGGGG
GCGCGGTCCGAGGGCGACATCGTTCTGGGCCTCGACACAAATCTGATGGCCGCAGCTTTGGACGCGGGCCTCATCGCCGA
GCATGACAGCACGCCGGGCGATCTGACCCTTCCGATGACCTGGGATGACCCCCACTTCATGCCGTTCGACTGGGGCTATT
TCGCCTTCGTACACCGCACGGATATGGAGACCGTGCCAGGCAGCTTTGAGGACCTCGCAGCCTCCGACGCATCCATCGTG
ATCCAGGATCCCCGGTCCTCCACTCCCGGCCTTGGCCTTCTAATGTGGGTGCAGAACGCCTACGGAGACGAGGCCGCCGC
AATCTGGGAAGGACTTGCCGATAACGTCGTTACGGTGACGCCCGGCTGGTCCGAAGCCTATGGCCTGTTCTTGGAGGGTG
AGGCGGATATGGTCCTGTCCTATACGACCTCTCCGGCGTATCACCTGATCGCGGAGGACGACGCCGGGTTTGAGGCTGCC
GCCTTTGCGGAGGGGCATTACATGCAGATCGAGGTGGGCGGCATCTTGCAGACCGCTGACAACCCGGACCTGGCGCAACA
ATTCCTCGACTTCATGATCTCACCCGCCTTCCAGACGGTGATTCCGGAGACCAACTGGATGTACCCCGCCGCCCTGCCGG
TGGACCAGTTGCCCGAGGGGTTCGAGACGCTCATCCAACCCGAGACCGCGTTGATTTTCACCCCTGAAGAGGCCGCCGCC
ATCCGCGACGACGCATTGGCGACATGGCAAAACGCGCTCAGCCAATAA

Upstream 100 bases:

>100_bases
TGACCCGTTGAACCTGAACCGGTTAATACCGGCGGAGGGAAGGGTATCGCGGCCATCGCCCCGGACTTCACTTCGCCCCT
AGCTTTGGAGGAGTGAGGAC

Downstream 100 bases:

>100_bases
CGCTGGTGCAGGGGATCGGCGGGGCGTTTGTCCTGCTGGTTATCCTCGCGATCCTCGGCCCCCTCGGCGCAGTGATGATC
CGGGCTGAGGGGGTCAATGG

Product: thiamine transporter substrate binding subunit

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKLTNTTILTAGITAGLTLLTGAAFAQVPVLTVYTYDSFNSEWGPGPGVELAFEEICECDLQFTSTGDGAALLARLRLEG
ARSEGDIVLGLDTNLMAAALDAGLIAEHDSTPGDLTLPMTWDDPHFMPFDWGYFAFVHRTDMETVPGSFEDLAASDASIV
IQDPRSSTPGLGLLMWVQNAYGDEAAAIWEGLADNVVTVTPGWSEAYGLFLEGEADMVLSYTTSPAYHLIAEDDAGFEAA
AFAEGHYMQIEVGGILQTADNPDLAQQFLDFMISPAFQTVIPETNWMYPAALPVDQLPEGFETLIQPETALIFTPEEAAA
IRDDALATWQNALSQ

Sequences:

>Translated_335_residues
MKLTNTTILTAGITAGLTLLTGAAFAQVPVLTVYTYDSFNSEWGPGPGVELAFEEICECDLQFTSTGDGAALLARLRLEG
ARSEGDIVLGLDTNLMAAALDAGLIAEHDSTPGDLTLPMTWDDPHFMPFDWGYFAFVHRTDMETVPGSFEDLAASDASIV
IQDPRSSTPGLGLLMWVQNAYGDEAAAIWEGLADNVVTVTPGWSEAYGLFLEGEADMVLSYTTSPAYHLIAEDDAGFEAA
AFAEGHYMQIEVGGILQTADNPDLAQQFLDFMISPAFQTVIPETNWMYPAALPVDQLPEGFETLIQPETALIFTPEEAAA
IRDDALATWQNALSQ
>Mature_335_residues
MKLTNTTILTAGITAGLTLLTGAAFAQVPVLTVYTYDSFNSEWGPGPGVELAFEEICECDLQFTSTGDGAALLARLRLEG
ARSEGDIVLGLDTNLMAAALDAGLIAEHDSTPGDLTLPMTWDDPHFMPFDWGYFAFVHRTDMETVPGSFEDLAASDASIV
IQDPRSSTPGLGLLMWVQNAYGDEAAAIWEGLADNVVTVTPGWSEAYGLFLEGEADMVLSYTTSPAYHLIAEDDAGFEAA
AFAEGHYMQIEVGGILQTADNPDLAQQFLDFMISPAFQTVIPETNWMYPAALPVDQLPEGFETLIQPETALIFTPEEAAA
IRDDALATWQNALSQ

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=318, Percent_Identity=52.5157232704402, Blast_Score=345, Evalue=3e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36050; Mature: 36050

Theoretical pI: Translated: 3.51; Mature: 3.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTNTTILTAGITAGLTLLTGAAFAQVPVLTVYTYDSFNSEWGPGPGVELAFEEICECD
CCCCCCEEEECCHHHHHHHHHCCHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCC
LQFTSTGDGAALLARLRLEGARSEGDIVLGLDTNLMAAALDAGLIAEHDSTPGDLTLPMT
EEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCEEECCCCCCCCEEEEEE
WDDPHFMPFDWGYFAFVHRTDMETVPGSFEDLAASDASIVIQDPRSSTPGLGLLMWVQNA
CCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCEEEEECCCCCCCCCEEEEEEHHC
YGDEAAAIWEGLADNVVTVTPGWSEAYGLFLEGEADMVLSYTTSPAYHLIAEDDAGFEAA
CCCHHHHHHHHHCCCEEEECCCCCHHEEEEEECCCCEEEEEECCCEEEEEECCCCCCEEE
AFAEGHYMQIEVGGILQTADNPDLAQQFLDFMISPAFQTVIPETNWMYPAALPVDQLPEG
EECCCCEEEEEECEEEEECCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCHHHCCHH
FETLIQPETALIFTPEEAAAIRDDALATWQNALSQ
HHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLTNTTILTAGITAGLTLLTGAAFAQVPVLTVYTYDSFNSEWGPGPGVELAFEEICECD
CCCCCCEEEECCHHHHHHHHHCCHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHHHCCC
LQFTSTGDGAALLARLRLEGARSEGDIVLGLDTNLMAAALDAGLIAEHDSTPGDLTLPMT
EEEECCCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCEEECCCCCCCCEEEEEE
WDDPHFMPFDWGYFAFVHRTDMETVPGSFEDLAASDASIVIQDPRSSTPGLGLLMWVQNA
CCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCEEEEECCCCCCCCCEEEEEEHHC
YGDEAAAIWEGLADNVVTVTPGWSEAYGLFLEGEADMVLSYTTSPAYHLIAEDDAGFEAA
CCCHHHHHHHHHCCCEEEECCCCCHHEEEEEECCCCEEEEEECCCEEEEEECCCCCCEEE
AFAEGHYMQIEVGGILQTADNPDLAQQFLDFMISPAFQTVIPETNWMYPAALPVDQLPEG
EECCCCEEEEEECEEEEECCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCHHHCCHH
FETLIQPETALIFTPEEAAAIRDDALATWQNALSQ
HHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]