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The map label for this gene is rlmN [H]

Identifier: 89052760

GI number: 89052760

Start: 277098

End: 278330

Strand: Direct

Name: rlmN [H]

Synonym: Jann_0269

Alternate gene names: 89052760

Gene position: 277098-278330 (Clockwise)

Preceding gene: 89052759

Following gene: 89052768

Centisome position: 6.42

GC content: 59.37

Gene sequence:

>1233_bases
GTGGACGCGTGGCCTCCTATATGGGAAGCCAATCCTAGGAAGAGTATTGTTGTCATGACCGCCACTGCGCCTATCACGCA
AGACGTTCGCACGTTTCCGCGCAAGTTGCCCGACGGGCCGGTCAATTTGATCGGTCTCACCCGCGACGGTCTGCGCGCCG
CGTTGATTGAGGCCGGGACACCGGAAAAACAGGCCAAGATGCGCACGGGACAGATCTGGCAATGGCTGTACCAGAAGGGC
GTGCGCGATTTTGCGTCGATGACGAATCTGTCCAAGGATTACCGTGCGATGCTGGCCGAGACCTTTGTGGCTGATGTGCC
TGAGGTTGTGTCCAGGCAGGTCAGCGCCGATGGAACCCGCAAGTATCTGGTCCGCATCGCAGGTGGCCATGAGGTGGAGG
TCGTCTACATCCCCGAAGTGGATCGCGGTACGCTGTGTATTTCCAGCCAGGTCGGGTGCACGCTGACCTGCTCGTTTTGT
CACACCGGCACGCAAAAACTGGTTCGTAACCTGACCGCGGGGGAGATCATTGGCCAGGTCATGTTGGCTCGGGATGATCT
GGACGAATGGGTGCCGACGGGTGAAGGCAGCGACGCAAAGCCGCGTCTGGTGTCCAACATCGTTCTGATGGGCATGGGCG
AGCCGCTCTATAATTTTGAGAACGTTCGCGACGCGATGAAGATCGCGATGGATCCGGAAGGCATCAGCCTCTCGCGGCGC
AGGATCACGCTGTCCACTTCTGGCGTGGTGCCCGAGATTGCGAAAACCGCGCAGGAGATCGGATGTCAGTTGGCCGTGTC
CTTCCACGCCACAACCGATGACGTGCGCGACAAGCTGGTCCCGATCAACAAGCGCTGGCCGATTGCCGATTTGCTGGATG
CTTTGCGCGACTACCCTCGCGTCAGCAACTCAGAGCGGATCACGTTCGAATATGTGATGCTGAAGGACGTCAACGACAGC
GACGCGGATGCACGGCGTCTGGTGCAGTTGATCAAGGGGATCCCGGCGAAGATCAACCTGATCCCGTTCAACGAATGGCC
GGGCGCGCCCTATGAGCGGTCTGACTGGGCCCGGATCGAAGCGTTTGCAGATATTATCTACAAGGCTGGCTATGCCTCTC
CCATCCGCACGCCCCGTGGGGAGGATATCATGGCTGCTTGTGGTCAGTTGAAGTCTGCCACGGAACGCGCCCGGAAGTCT
CGCAAGCAGATTGAGGCGGAAGCCGGGCTATAA

Upstream 100 bases:

>100_bases
CTCCACGCGCGGGACGGAGGTTGCCGACACCTTCAACCCGCAAGGCTTCGCGCTGGCCATGGATGACGCCCAGGTCCGTT
GCGCGCCCTGAGGGGTTTGC

Downstream 100 bases:

>100_bases
ACACCATCCTTCCACGAAAAAAGGCGCAACCCGTGAAGGTCGCGCCTTTCCAATGTCGTGCAACTTGATCCGGAGATCAG
TTGTGGTGCAGGGCCAGAAC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MDAWPPIWEANPRKSIVVMTATAPITQDVRTFPRKLPDGPVNLIGLTRDGLRAALIEAGTPEKQAKMRTGQIWQWLYQKG
VRDFASMTNLSKDYRAMLAETFVADVPEVVSRQVSADGTRKYLVRIAGGHEVEVVYIPEVDRGTLCISSQVGCTLTCSFC
HTGTQKLVRNLTAGEIIGQVMLARDDLDEWVPTGEGSDAKPRLVSNIVLMGMGEPLYNFENVRDAMKIAMDPEGISLSRR
RITLSTSGVVPEIAKTAQEIGCQLAVSFHATTDDVRDKLVPINKRWPIADLLDALRDYPRVSNSERITFEYVMLKDVNDS
DADARRLVQLIKGIPAKINLIPFNEWPGAPYERSDWARIEAFADIIYKAGYASPIRTPRGEDIMAACGQLKSATERARKS
RKQIEAEAGL

Sequences:

>Translated_410_residues
MDAWPPIWEANPRKSIVVMTATAPITQDVRTFPRKLPDGPVNLIGLTRDGLRAALIEAGTPEKQAKMRTGQIWQWLYQKG
VRDFASMTNLSKDYRAMLAETFVADVPEVVSRQVSADGTRKYLVRIAGGHEVEVVYIPEVDRGTLCISSQVGCTLTCSFC
HTGTQKLVRNLTAGEIIGQVMLARDDLDEWVPTGEGSDAKPRLVSNIVLMGMGEPLYNFENVRDAMKIAMDPEGISLSRR
RITLSTSGVVPEIAKTAQEIGCQLAVSFHATTDDVRDKLVPINKRWPIADLLDALRDYPRVSNSERITFEYVMLKDVNDS
DADARRLVQLIKGIPAKINLIPFNEWPGAPYERSDWARIEAFADIIYKAGYASPIRTPRGEDIMAACGQLKSATERARKS
RKQIEAEAGL
>Mature_410_residues
MDAWPPIWEANPRKSIVVMTATAPITQDVRTFPRKLPDGPVNLIGLTRDGLRAALIEAGTPEKQAKMRTGQIWQWLYQKG
VRDFASMTNLSKDYRAMLAETFVADVPEVVSRQVSADGTRKYLVRIAGGHEVEVVYIPEVDRGTLCISSQVGCTLTCSFC
HTGTQKLVRNLTAGEIIGQVMLARDDLDEWVPTGEGSDAKPRLVSNIVLMGMGEPLYNFENVRDAMKIAMDPEGISLSRR
RITLSTSGVVPEIAKTAQEIGCQLAVSFHATTDDVRDKLVPINKRWPIADLLDALRDYPRVSNSERITFEYVMLKDVNDS
DADARRLVQLIKGIPAKINLIPFNEWPGAPYERSDWARIEAFADIIYKAGYASPIRTPRGEDIMAACGQLKSATERARKS
RKQIEAEAGL

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=375, Percent_Identity=44.8, Blast_Score=309, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 45534; Mature: 45534

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAWPPIWEANPRKSIVVMTATAPITQDVRTFPRKLPDGPVNLIGLTRDGLRAALIEAGT
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCC
PEKQAKMRTGQIWQWLYQKGVRDFASMTNLSKDYRAMLAETFVADVPEVVSRQVSADGTR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
KYLVRIAGGHEVEVVYIPEVDRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTAGEIIGQV
EEEEEEECCCEEEEEECCCCCCCCEEEECCCCCEEEEHHHHCCHHHHHHHCHHHHHHHHH
MLARDDLDEWVPTGEGSDAKPRLVSNIVLMGMGEPLYNFENVRDAMKIAMDPEGISLSRR
HHHHHHHHHHCCCCCCCCCCCHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCCCCCEECE
RITLSTSGVVPEIAKTAQEIGCQLAVSFHATTDDVRDKLVPINKRWPIADLLDALRDYPR
EEEEECCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHCCC
VSNSERITFEYVMLKDVNDSDADARRLVQLIKGIPAKINLIPFNEWPGAPYERSDWARIE
CCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHH
AFADIIYKAGYASPIRTPRGEDIMAACGQLKSATERARKSRKQIEAEAGL
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDAWPPIWEANPRKSIVVMTATAPITQDVRTFPRKLPDGPVNLIGLTRDGLRAALIEAGT
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCC
PEKQAKMRTGQIWQWLYQKGVRDFASMTNLSKDYRAMLAETFVADVPEVVSRQVSADGTR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
KYLVRIAGGHEVEVVYIPEVDRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTAGEIIGQV
EEEEEEECCCEEEEEECCCCCCCCEEEECCCCCEEEEHHHHCCHHHHHHHCHHHHHHHHH
MLARDDLDEWVPTGEGSDAKPRLVSNIVLMGMGEPLYNFENVRDAMKIAMDPEGISLSRR
HHHHHHHHHHCCCCCCCCCCCHHHHHHHEEECCCCHHHHHHHHHHHHHHCCCCCCCEECE
RITLSTSGVVPEIAKTAQEIGCQLAVSFHATTDDVRDKLVPINKRWPIADLLDALRDYPR
EEEEECCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHCCC
VSNSERITFEYVMLKDVNDSDADARRLVQLIKGIPAKINLIPFNEWPGAPYERSDWARIE
CCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHH
AFADIIYKAGYASPIRTPRGEDIMAACGQLKSATERARKSRKQIEAEAGL
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA