Click here to switch to the map view.

The map label for this gene is rph [H]

Identifier: 89052697

GI number: 89052697

Start: 197503

End: 198132

Strand: Reverse

Name: rph [H]

Synonym: Jann_0206

Alternate gene names: 89052697

Gene position: 198132-197503 (Counterclockwise)

Preceding gene: 89052700

Following gene: 89052696

Centisome position: 4.59

GC content: 60.79

Gene sequence:

>630_bases
ATGATCCGTTGCGGTGATACGCACGTTCTGTGCACCGCGACTTTGGAGGCGCGTGTGCCGCCGTTTCTGAAGAATTCGGG
CCTCGGTTGGGTGACGGCAGAATATGGAATGCTGCCCCGCGCCACACACACACGCATGCGTCGCGAGGCGGCAGCGGGCA
AGCAATCAGGTCGAACGCAGGAGATCCAACGGCTGATTGGCCGGAGTCTGCGCGCTGGAATTGATCGATCTGCCCTTGGG
GAGCGTCAAATCACCGTGGATTGCGATGTAATCCAGGCCGATGGCGGGACCCGGTGCGCGTCGATCACCGGCGGTTGGGT
CGCGCTGCGTTTGGCTGTGAATAAATTGCTGAAATCCGGTGAAATCGTGACCGACCCGATCGTCGACCATATCGCCGCTG
TGTCTTGCGGGATTTATGCTGGCCAACCTGTCCTGGACCTGGATTACCCTGAGGATTCCGAGGCGGGGACAGACGGAAAC
TTCATCATGACGGGTGCCGGTCAGGTCGTCGAGTTGCAAGCCAGCGCGGAGGGGGCGACGTTCTCCCGGGATGAGTTCAA
CGAACTTTACAGCCTGGCCGAAAAGGGTGTGGCAGAACTGGTTCAGGCGCAGGCCGCGGCAACCGCCTGA

Upstream 100 bases:

>100_bases
GTATCAGGAGATATTTATGCGTCCTTCCGGCCGAAACCTAGACGAAATGCGCGCTATTTCCATTGAAACAGATGTGACGC
GCCATGCAGAGGGATCTTGC

Downstream 100 bases:

>100_bases
TGCGACGCTTTACCGGGGACCAACTTCTGATCGCGACCCACAATCAGGGCAAGCTGGACGAGATGCGGCATCTTTTGCAG
CCGTTTGGGATCCGCGTCTC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MIRCGDTHVLCTATLEARVPPFLKNSGLGWVTAEYGMLPRATHTRMRREAAAGKQSGRTQEIQRLIGRSLRAGIDRSALG
ERQITVDCDVIQADGGTRCASITGGWVALRLAVNKLLKSGEIVTDPIVDHIAAVSCGIYAGQPVLDLDYPEDSEAGTDGN
FIMTGAGQVVELQASAEGATFSRDEFNELYSLAEKGVAELVQAQAAATA

Sequences:

>Translated_209_residues
MIRCGDTHVLCTATLEARVPPFLKNSGLGWVTAEYGMLPRATHTRMRREAAAGKQSGRTQEIQRLIGRSLRAGIDRSALG
ERQITVDCDVIQADGGTRCASITGGWVALRLAVNKLLKSGEIVTDPIVDHIAAVSCGIYAGQPVLDLDYPEDSEAGTDGN
FIMTGAGQVVELQASAEGATFSRDEFNELYSLAEKGVAELVQAQAAATA
>Mature_209_residues
MIRCGDTHVLCTATLEARVPPFLKNSGLGWVTAEYGMLPRATHTRMRREAAAGKQSGRTQEIQRLIGRSLRAGIDRSALG
ERQITVDCDVIQADGGTRCASITGGWVALRLAVNKLLKSGEIVTDPIVDHIAAVSCGIYAGQPVLDLDYPEDSEAGTDGN
FIMTGAGQVVELQASAEGATFSRDEFNELYSLAEKGVAELVQAQAAATA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=182, Percent_Identity=59.8901098901099, Blast_Score=219, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 22178; Mature: 22178

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRCGDTHVLCTATLEARVPPFLKNSGLGWVTAEYGMLPRATHTRMRREAAAGKQSGRTQ
CCCCCCCEEEEEEEECCCCCCCHHCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCHH
EIQRLIGRSLRAGIDRSALGERQITVDCDVIQADGGTRCASITGGWVALRLAVNKLLKSG
HHHHHHHHHHHCCCCHHHCCCCEEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHCC
EIVTDPIVDHIAAVSCGIYAGQPVLDLDYPEDSEAGTDGNFIMTGAGQVVELQASAEGAT
CEEHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCC
FSRDEFNELYSLAEKGVAELVQAQAAATA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRCGDTHVLCTATLEARVPPFLKNSGLGWVTAEYGMLPRATHTRMRREAAAGKQSGRTQ
CCCCCCCEEEEEEEECCCCCCCHHCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCHH
EIQRLIGRSLRAGIDRSALGERQITVDCDVIQADGGTRCASITGGWVALRLAVNKLLKSG
HHHHHHHHHHHCCCCHHHCCCCEEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHCC
EIVTDPIVDHIAAVSCGIYAGQPVLDLDYPEDSEAGTDGNFIMTGAGQVVELQASAEGAT
CEEHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCC
FSRDEFNELYSLAEKGVAELVQAQAAATA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA