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The map label for this gene is mnmG

Identifier: 89052691

GI number: 89052691

Start: 191549

End: 193423

Strand: Direct

Name: mnmG

Synonym: Jann_0200

Alternate gene names: 89052691

Gene position: 191549-193423 (Clockwise)

Preceding gene: 89052690

Following gene: 89052692

Centisome position: 4.44

GC content: 53.07

Gene sequence:

>1875_bases
GTGTTTCACGTGAAACATCGGCACTTTGATGTGATTGTGGTGGGCGGTGGACACGCTGGAGTGGAAGCGGCTGACGCAGC
GTGTCGTGCAGGCGCACGCACGGCGTTGGTAACGTTGCGAAAAGACGACCTGGGTGTGATGTCCTGCAACCCCGCAATTG
GTGGCCTGGGTAAGGGTCACTTGGTACGTGAGATTGATGCCATGGGTGGTGTCATGGGGTGCGCTGCTGATGCTTCGGGT
ATTCAATATCGCTTGTTGAATCGGAAAAAAGGGGCCGCTGTTCAAGGACCGCGAACCCAATCTGACAGGGCGTTATACAA
GTCAGCCATCCAGGATCACATGCGGAACGTCACTGAATTGACTGTGATTGAGGGCGAGGTTGCAGACGTAATCGTTGAGA
GTGAAACAGTTTGCGGCGTTTCGTTTACAGATGGCCAGAGCATAAGCGCGAGATCTGTCGTTTTGACGACGGGCACCTTT
TTACGTGGCGTGATCCATATTGGGCATGAGACGCGGTCTGGTGGCCGGTTTGGAGCAAATCGGGCCATCAAGCTAGCGGA
GCGATTGTCCGCGATGAATTTGCCGGTCGGTCGGTTGAAAACCGGGACGCCACCCCGTCTTGCGGCGAAGTCAATCAATT
GGGAGGCTTTGGACCACCAGCCATCAGACGACGATCCCACCTTTTTTTCGTTTCTAACAAAACGTGTGAGAGCCCGACAA
ATTGCATGCGGCATTACGCATACAAACCCCCATACCCATGACATTATAGAACAAAACCTTGGTCGTTCGGCAATGTACGG
GGGTAATATCGACAGCGTTGGGCCAAGATACTGCCCGTCGATTGAGGACAAGGTTGTTCGCTTCGCCGATAAGGTCTCGC
ACCAGATATTCCTTGAACCGGAAGGTCTGACATCTGAAACCGTCTACCCGAACGGTATCTCAACCTCGCTGCCAATAGAC
GTTCAACTTGATTATGTTCATTCCATCAAGGGTTTGGAGCACGCAGATATATTGCAGCCAGGATATGCAATCGAATATGA
CTACATAGATCCAACCTGTTTGAACAACCGACTTGAACTTCAGGATCATCCCGGACTTTTTCTGGCCGGGCAGATCAACG
GAACGACAGGGTACGAGGAAGCCGCCGCGCAGGGTTTGGTTGCGGGATTGAACGCCGCGTTCCATGCGCAGGATCGTGAT
CCGCTGACATTTAGCAGACGCGAGTCGTATATCGGCGTTCTCGTTGATGACCTTGTAACGCGGGGTGTTTCGGAGCCCTA
CAGGATGTTCACATCGCGTGCGGAGTACAGACTTTCGTTGAGGGCAGACAACGCAGACCAACGATTGACGCCCATCGCAG
CGGATCTTGATTTGTTGACGGAAGAGAGAACTTCTGAATTTACCGCCAAAATGGAGCGGCTATCGCGCGGCTACGATATC
CTGAATGATCTAGTGGTAACGTCCAAGCAAGCTGCCGCGGCGGGTATGAGCGTAAACGCGGACGGAAAAAAGCGGACTGG
GGCTGAGCTACTAGCATTCCCATCGGTAGAACTTAGTGATATCGCGCAGCTCGAGCCCGCCGTTGGTCAGATCGATCCCG
CAACGGCCAAACAGCTCAAGATAGAAGCAATCTACGCCGTCTATATCGAACGTCAAAACCAGGATGCATTCGCGTTGAAG
CGAGATGAAGCAAAATCGATACCGGCGGACTTTCCGTATTCGTCTGTGGTTGGTCTATCGAACGAATTGCGGATGAAACT
GATAAAGACGCGTCCATCCACGTTGGCGCAAGCCAGCCGTATCGACGGCATGACGCCGGCAGCTCTTGCACTTATCCTAA
CGAAAATTCGACAGACCCAGCGCCTATCAGCATGA

Upstream 100 bases:

>100_bases
TTCGTCACGCACAGCGATCGCTTGACTCCGTTATTGGTCGTGTGGATGTAGAGAATCTCTTGGGTGAAATTTTTTCCCGA
TTTTGTATTGGGAAGTAGGA

Downstream 100 bases:

>100_bases
ACGAAAGCGACGCAAAACACTGGCTCTCTGATCGTGTTTCACGTGAAACAATGGACCGGTTGGAACTCTATCACACGCTC
CTTACCCGGTGGCAAAAGAC

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 624; Mature: 624

Protein sequence:

>624_residues
MFHVKHRHFDVIVVGGGHAGVEAADAACRAGARTALVTLRKDDLGVMSCNPAIGGLGKGHLVREIDAMGGVMGCAADASG
IQYRLLNRKKGAAVQGPRTQSDRALYKSAIQDHMRNVTELTVIEGEVADVIVESETVCGVSFTDGQSISARSVVLTTGTF
LRGVIHIGHETRSGGRFGANRAIKLAERLSAMNLPVGRLKTGTPPRLAAKSINWEALDHQPSDDDPTFFSFLTKRVRARQ
IACGITHTNPHTHDIIEQNLGRSAMYGGNIDSVGPRYCPSIEDKVVRFADKVSHQIFLEPEGLTSETVYPNGISTSLPID
VQLDYVHSIKGLEHADILQPGYAIEYDYIDPTCLNNRLELQDHPGLFLAGQINGTTGYEEAAAQGLVAGLNAAFHAQDRD
PLTFSRRESYIGVLVDDLVTRGVSEPYRMFTSRAEYRLSLRADNADQRLTPIAADLDLLTEERTSEFTAKMERLSRGYDI
LNDLVVTSKQAAAAGMSVNADGKKRTGAELLAFPSVELSDIAQLEPAVGQIDPATAKQLKIEAIYAVYIERQNQDAFALK
RDEAKSIPADFPYSSVVGLSNELRMKLIKTRPSTLAQASRIDGMTPAALALILTKIRQTQRLSA

Sequences:

>Translated_624_residues
MFHVKHRHFDVIVVGGGHAGVEAADAACRAGARTALVTLRKDDLGVMSCNPAIGGLGKGHLVREIDAMGGVMGCAADASG
IQYRLLNRKKGAAVQGPRTQSDRALYKSAIQDHMRNVTELTVIEGEVADVIVESETVCGVSFTDGQSISARSVVLTTGTF
LRGVIHIGHETRSGGRFGANRAIKLAERLSAMNLPVGRLKTGTPPRLAAKSINWEALDHQPSDDDPTFFSFLTKRVRARQ
IACGITHTNPHTHDIIEQNLGRSAMYGGNIDSVGPRYCPSIEDKVVRFADKVSHQIFLEPEGLTSETVYPNGISTSLPID
VQLDYVHSIKGLEHADILQPGYAIEYDYIDPTCLNNRLELQDHPGLFLAGQINGTTGYEEAAAQGLVAGLNAAFHAQDRD
PLTFSRRESYIGVLVDDLVTRGVSEPYRMFTSRAEYRLSLRADNADQRLTPIAADLDLLTEERTSEFTAKMERLSRGYDI
LNDLVVTSKQAAAAGMSVNADGKKRTGAELLAFPSVELSDIAQLEPAVGQIDPATAKQLKIEAIYAVYIERQNQDAFALK
RDEAKSIPADFPYSSVVGLSNELRMKLIKTRPSTLAQASRIDGMTPAALALILTKIRQTQRLSA
>Mature_624_residues
MFHVKHRHFDVIVVGGGHAGVEAADAACRAGARTALVTLRKDDLGVMSCNPAIGGLGKGHLVREIDAMGGVMGCAADASG
IQYRLLNRKKGAAVQGPRTQSDRALYKSAIQDHMRNVTELTVIEGEVADVIVESETVCGVSFTDGQSISARSVVLTTGTF
LRGVIHIGHETRSGGRFGANRAIKLAERLSAMNLPVGRLKTGTPPRLAAKSINWEALDHQPSDDDPTFFSFLTKRVRARQ
IACGITHTNPHTHDIIEQNLGRSAMYGGNIDSVGPRYCPSIEDKVVRFADKVSHQIFLEPEGLTSETVYPNGISTSLPID
VQLDYVHSIKGLEHADILQPGYAIEYDYIDPTCLNNRLELQDHPGLFLAGQINGTTGYEEAAAQGLVAGLNAAFHAQDRD
PLTFSRRESYIGVLVDDLVTRGVSEPYRMFTSRAEYRLSLRADNADQRLTPIAADLDLLTEERTSEFTAKMERLSRGYDI
LNDLVVTSKQAAAAGMSVNADGKKRTGAELLAFPSVELSDIAQLEPAVGQIDPATAKQLKIEAIYAVYIERQNQDAFALK
RDEAKSIPADFPYSSVVGLSNELRMKLIKTRPSTLAQASRIDGMTPAALALILTKIRQTQRLSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=635, Percent_Identity=45.6692913385827, Blast_Score=511, Evalue=1e-145,
Organism=Homo sapiens, GI19882217, Length=660, Percent_Identity=43.9393939393939, Blast_Score=496, Evalue=1e-140,
Organism=Homo sapiens, GI183227703, Length=675, Percent_Identity=42.962962962963, Blast_Score=491, Evalue=1e-139,
Organism=Escherichia coli, GI2367273, Length=617, Percent_Identity=51.3776337115073, Blast_Score=595, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI17534255, Length=615, Percent_Identity=41.6260162601626, Blast_Score=464, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6321202, Length=617, Percent_Identity=43.7601296596434, Blast_Score=505, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24658174, Length=630, Percent_Identity=45.7142857142857, Blast_Score=509, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_JANSC (Q28VZ5)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508142.1
- ProteinModelPortal:   Q28VZ5
- SMR:   Q28VZ5
- STRING:   Q28VZ5
- GeneID:   3932637
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0200
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- PhylomeDB:   Q28VZ5
- ProtClustDB:   PRK05192
- BioCyc:   JSP290400:JANN_0200-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 67746; Mature: 67746

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHVKHRHFDVIVVGGGHAGVEAADAACRAGARTALVTLRKDDLGVMSCNPAIGGLGKGH
CCCEEEEEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCCCH
LVREIDAMGGVMGCAADASGIQYRLLNRKKGAAVQGPRTQSDRALYKSAIQDHMRNVTEL
HHHHHHHHCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEE
TVIEGEVADVIVESETVCGVSFTDGQSISARSVVLTTGTFLRGVIHIGHETRSGGRFGAN
EEECCCHHEEEEECCEEEEEEECCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCH
RAIKLAERLSAMNLPVGRLKTGTPPRLAAKSINWEALDHQPSDDDPTFFSFLTKRVRARQ
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHH
IACGITHTNPHTHDIIEQNLGRSAMYGGNIDSVGPRYCPSIEDKVVRFADKVSHQIFLEP
HEEEEECCCCCHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECC
EGLTSETVYPNGISTSLPIDVQLDYVHSIKGLEHADILQPGYAIEYDYIDPTCLNNRLEL
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCHHHHCCCCCEEEECCCCHHHCCCEEEE
QDHPGLFLAGQINGTTGYEEAAAQGLVAGLNAAFHAQDRDPLTFSRRESYIGVLVDDLVT
CCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHEECCCCCCCEECCCHHHHHHHHHHHHH
RGVSEPYRMFTSRAEYRLSLRADNADQRLTPIAADLDLLTEERTSEFTAKMERLSRGYDI
CCCCHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNDLVVTSKQAAAAGMSVNADGKKRTGAELLAFPSVELSDIAQLEPAVGQIDPATAKQLK
HHHHHHCCCHHHHCCCEECCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCHHHHEE
IEAIYAVYIERQNQDAFALKRDEAKSIPADFPYSSVVGLSNELRMKLIKTRPSTLAQASR
HEEEEEEEEEECCCCCEEEECCHHCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHH
IDGMTPAALALILTKIRQTQRLSA
CCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFHVKHRHFDVIVVGGGHAGVEAADAACRAGARTALVTLRKDDLGVMSCNPAIGGLGKGH
CCCEEEEEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCCCH
LVREIDAMGGVMGCAADASGIQYRLLNRKKGAAVQGPRTQSDRALYKSAIQDHMRNVTEL
HHHHHHHHCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEE
TVIEGEVADVIVESETVCGVSFTDGQSISARSVVLTTGTFLRGVIHIGHETRSGGRFGAN
EEECCCHHEEEEECCEEEEEEECCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCH
RAIKLAERLSAMNLPVGRLKTGTPPRLAAKSINWEALDHQPSDDDPTFFSFLTKRVRARQ
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHH
IACGITHTNPHTHDIIEQNLGRSAMYGGNIDSVGPRYCPSIEDKVVRFADKVSHQIFLEP
HEEEEECCCCCHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECC
EGLTSETVYPNGISTSLPIDVQLDYVHSIKGLEHADILQPGYAIEYDYIDPTCLNNRLEL
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCHHHHCCCCCEEEECCCCHHHCCCEEEE
QDHPGLFLAGQINGTTGYEEAAAQGLVAGLNAAFHAQDRDPLTFSRRESYIGVLVDDLVT
CCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHEECCCCCCCEECCCHHHHHHHHHHHHH
RGVSEPYRMFTSRAEYRLSLRADNADQRLTPIAADLDLLTEERTSEFTAKMERLSRGYDI
CCCCHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNDLVVTSKQAAAAGMSVNADGKKRTGAELLAFPSVELSDIAQLEPAVGQIDPATAKQLK
HHHHHHCCCHHHHCCCEECCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCCHHHHEE
IEAIYAVYIERQNQDAFALKRDEAKSIPADFPYSSVVGLSNELRMKLIKTRPSTLAQASR
HEEEEEEEEEECCCCCEEEECCHHCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHH
IDGMTPAALALILTKIRQTQRLSA
CCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA