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The map label for this gene is trmE

Identifier: 89052690

GI number: 89052690

Start: 190266

End: 191546

Strand: Direct

Name: trmE

Synonym: Jann_0199

Alternate gene names: 89052690

Gene position: 190266-191546 (Clockwise)

Preceding gene: 89052689

Following gene: 89052691

Centisome position: 4.41

GC content: 54.88

Gene sequence:

>1281_bases
GTGCAGACGATTTTCGCTCAGGCAACGGCCCGGGGGAAAGCGGGCGTTGCGATTATTCGAATTTCGGGACCAGATGCGTT
TGAAGTTGGGCGGTGTCTGCTGGGCTCGCTTCCTGACGCGGGCCGGTTTGCTCTTCGCGACGTGAGGACGCCGCTGGGTG
AATTACTGGATCGTGGTCTCGTGTTGGTATTTAAGGCTCCAGCCAGCTTTACCGGTGAAGACACGGTTGAACTGCAGCTC
CACGGCAGTGTTGCGGTGGTGCGGGCTGTTGAGGGCGCAATTCGAAGCACAGGTCTGGCACGGCTCGCTGATGCTGGAGA
GTTTACACAGCGGGCGTTGTTGAACGATATGTTGGACCTGACCCAGGTTGAAGGACTTGGCCGCTTAATCGATTCAGAGA
CAGAAGCTCAACGGAAAGTCGCTCAGGCGAGCTTCGAAGGTGGATTGTCAGACAAGGCTGAGCGTTGGCGGCACCTGATG
ATCCGGGCTGCGGCGCTGTTGGAGGCCTCCATAGACTTCGTTGATGAAGATGTGCCGGTTGATGTTGTCCCGGAGGTCCA
AAGCATCCTGATGGGCTTGGATACCGATTTAGCCAAGGAAGTCACGGGGGCCGTTGTGGCCGAACGTCTACACGACGGTT
TTGAAGTTGCGATTCTTGGGGCGCCCAACGCTGGAAAATCCACACTTCTCAACGTATTAGCGGATCGTGAGATAGCTATC
ACGTCAGATGTGCCTGGGACTACGCGGGATGTTATCGAAGCGCGCTTGGACGTTTCTGGATTGCCGGTCACCTTTTTGGA
CACGGCGGGCATACGGGATACCGTTGATGTAATAGAGAAGATAGGCGTTCAACGGGCGATTGATCGGGCGTTGGCGGCTG
ATGTGAGAATTCTTCTGGAAATAGATGACTGGATATTGCCGGACGTGCTATCCGATACAATCGACTTTCGGTACCGGGCG
AAGGCCGACTTGAGCGATGGTGAGGGTATCTCAGGGCGTACTGGCGTCGGTATCGATAGGTTGTTGACGGATATTGAAGG
CGTGCTGTCGGAGAAAATGAGTGCTGTCCGATCCGCTGTTACTGATCGTCAACGGGGTGGTATTGAGGCGGCAAGGGTCT
CTTTGGATGCCGGGGTAACGGTCTTGTCTGGGACCGCAGAGTTAGAGTTGGCTGCAGAACACATTCGTCACGCACAGCGA
TCGCTTGACTCCGTTATTGGTCGTGTGGATGTAGAGAATCTCTTGGGTGAAATTTTTTCCCGATTTTGTATTGGGAAGTA
G

Upstream 100 bases:

>100_bases
ATGGGCACGACGGACGCGATCGAATTCCTGATTTCCAAGTTGAAACAGACAAAAACGAACTCAGAATTCTTCGATTCCAT
GAATACCTGAGGAGTGTGTC

Downstream 100 bases:

>100_bases
GAGTGTTTCACGTGAAACATCGGCACTTTGATGTGATTGTGGTGGGCGGTGGACACGCTGGAGTGGAAGCGGCTGACGCA
GCGTGTCGTGCAGGCGCACG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MQTIFAQATARGKAGVAIIRISGPDAFEVGRCLLGSLPDAGRFALRDVRTPLGELLDRGLVLVFKAPASFTGEDTVELQL
HGSVAVVRAVEGAIRSTGLARLADAGEFTQRALLNDMLDLTQVEGLGRLIDSETEAQRKVAQASFEGGLSDKAERWRHLM
IRAAALLEASIDFVDEDVPVDVVPEVQSILMGLDTDLAKEVTGAVVAERLHDGFEVAILGAPNAGKSTLLNVLADREIAI
TSDVPGTTRDVIEARLDVSGLPVTFLDTAGIRDTVDVIEKIGVQRAIDRALAADVRILLEIDDWILPDVLSDTIDFRYRA
KADLSDGEGISGRTGVGIDRLLTDIEGVLSEKMSAVRSAVTDRQRGGIEAARVSLDAGVTVLSGTAELELAAEHIRHAQR
SLDSVIGRVDVENLLGEIFSRFCIGK

Sequences:

>Translated_426_residues
MQTIFAQATARGKAGVAIIRISGPDAFEVGRCLLGSLPDAGRFALRDVRTPLGELLDRGLVLVFKAPASFTGEDTVELQL
HGSVAVVRAVEGAIRSTGLARLADAGEFTQRALLNDMLDLTQVEGLGRLIDSETEAQRKVAQASFEGGLSDKAERWRHLM
IRAAALLEASIDFVDEDVPVDVVPEVQSILMGLDTDLAKEVTGAVVAERLHDGFEVAILGAPNAGKSTLLNVLADREIAI
TSDVPGTTRDVIEARLDVSGLPVTFLDTAGIRDTVDVIEKIGVQRAIDRALAADVRILLEIDDWILPDVLSDTIDFRYRA
KADLSDGEGISGRTGVGIDRLLTDIEGVLSEKMSAVRSAVTDRQRGGIEAARVSLDAGVTVLSGTAELELAAEHIRHAQR
SLDSVIGRVDVENLLGEIFSRFCIGK
>Mature_426_residues
MQTIFAQATARGKAGVAIIRISGPDAFEVGRCLLGSLPDAGRFALRDVRTPLGELLDRGLVLVFKAPASFTGEDTVELQL
HGSVAVVRAVEGAIRSTGLARLADAGEFTQRALLNDMLDLTQVEGLGRLIDSETEAQRKVAQASFEGGLSDKAERWRHLM
IRAAALLEASIDFVDEDVPVDVVPEVQSILMGLDTDLAKEVTGAVVAERLHDGFEVAILGAPNAGKSTLLNVLADREIAI
TSDVPGTTRDVIEARLDVSGLPVTFLDTAGIRDTVDVIEKIGVQRAIDRALAADVRILLEIDDWILPDVLSDTIDFRYRA
KADLSDGEGISGRTGVGIDRLLTDIEGVLSEKMSAVRSAVTDRQRGGIEAARVSLDAGVTVLSGTAELELAAEHIRHAQR
SLDSVIGRVDVENLLGEIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=465, Percent_Identity=37.2043010752688, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI193082993, Length=450, Percent_Identity=37.5555555555556, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI193082988, Length=497, Percent_Identity=34.6076458752515, Blast_Score=203, Evalue=3e-52,
Organism=Escherichia coli, GI2367268, Length=457, Percent_Identity=35.6673960612691, Blast_Score=231, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI17507259, Length=465, Percent_Identity=30.752688172043, Blast_Score=158, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6323665, Length=495, Percent_Identity=33.3333333333333, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI45550813, Length=470, Percent_Identity=34.2553191489362, Blast_Score=232, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_JANSC (Q28VZ6)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508141.1
- ProteinModelPortal:   Q28VZ6
- SMR:   Q28VZ6
- STRING:   Q28VZ6
- GeneID:   3932636
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0199
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   REIAITS
- PhylomeDB:   Q28VZ6
- ProtClustDB:   PRK05291
- BioCyc:   JSP290400:JANN_0199-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 45517; Mature: 45517

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: BINDING 20-20 BINDING 77-77 BINDING 117-117 BINDING 426-426

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTIFAQATARGKAGVAIIRISGPDAFEVGRCLLGSLPDAGRFALRDVRTPLGELLDRGL
CCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCE
VLVFKAPASFTGEDTVELQLHGSVAVVRAVEGAIRSTGLARLADAGEFTQRALLNDMLDL
EEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
TQVEGLGRLIDSETEAQRKVAQASFEGGLSDKAERWRHLMIRAAALLEASIDFVDEDVPV
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DVVPEVQSILMGLDTDLAKEVTGAVVAERLHDGFEVAILGAPNAGKSTLLNVLADREIAI
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCEEE
TSDVPGTTRDVIEARLDVSGLPVTFLDTAGIRDTVDVIEKIGVQRAIDRALAADVRILLE
ECCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
IDDWILPDVLSDTIDFRYRAKADLSDGEGISGRTGVGIDRLLTDIEGVLSEKMSAVRSAV
ECCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TDRQRGGIEAARVSLDAGVTVLSGTAELELAAEHIRHAQRSLDSVIGRVDVENLLGEIFS
HHHHHCCCHHHEEEHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
RFCIGK
HHHCCC
>Mature Secondary Structure
MQTIFAQATARGKAGVAIIRISGPDAFEVGRCLLGSLPDAGRFALRDVRTPLGELLDRGL
CCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCE
VLVFKAPASFTGEDTVELQLHGSVAVVRAVEGAIRSTGLARLADAGEFTQRALLNDMLDL
EEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
TQVEGLGRLIDSETEAQRKVAQASFEGGLSDKAERWRHLMIRAAALLEASIDFVDEDVPV
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DVVPEVQSILMGLDTDLAKEVTGAVVAERLHDGFEVAILGAPNAGKSTLLNVLADREIAI
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCEEE
TSDVPGTTRDVIEARLDVSGLPVTFLDTAGIRDTVDVIEKIGVQRAIDRALAADVRILLE
ECCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
IDDWILPDVLSDTIDFRYRAKADLSDGEGISGRTGVGIDRLLTDIEGVLSEKMSAVRSAV
ECCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TDRQRGGIEAARVSLDAGVTVLSGTAELELAAEHIRHAQRSLDSVIGRVDVENLLGEIFS
HHHHHCCCHHHEEEHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
RFCIGK
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA