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The map label for this gene is leuB

Identifier: 89052619

GI number: 89052619

Start: 117998

End: 119098

Strand: Direct

Name: leuB

Synonym: Jann_0128

Alternate gene names: 89052619

Gene position: 117998-119098 (Clockwise)

Preceding gene: 89052618

Following gene: 89052620

Centisome position: 2.73

GC content: 63.12

Gene sequence:

>1101_bases
ATGACTGACCGCTCTTTGCTTATCCTTCCCGGCGACGGGATCGGCCCCGAGGTGATGGCCGAGGTGCGGAAGATCATTGA
TTGGTTCGGCTCTGCCCGTGGCATCAGCTTCGATGTGTCCGAAGATCTTGTGGGCGGGGCGGCCTATGACGCCCACGGCG
TGCCGCTGACGGATGCCACGATGGAAAAGGCGCAAAGCGTCGATGCGGTTCTGCTCGGCGCTGTTGGTGGGCCCGCCTAT
GACGATCTGGATTTCTCGGTGAAGCCGGAACGGGGCCTGCTGCGCCTGCGCAAGGAGATGGATCTGTATGCCAACCTGCG
CCCTGCCCAGTGTTTTGATGCTCTGGCCGATTTCTCGTCACTGAAGCGCGAGATTGTCTCGGGCCTCGATATCATGATCC
TGCGGGAGCTGACGTCCGGCGTCTATTTCGGGGAGCCGCGCGGTATCCACACCGAGGGCAATGAGCGTGTGGGCATCAAC
ACCCAGCGCTATACGGAAACCGAGATTGCGCGCGCGGCACACTCTGCATTTGAACTGGCGCGGCGCCGCAACAACAAGGT
CTGCTCCATGGAGAAGGCCAACGTGATGGAGAGCGGTATCCTGTGGCGTGATGTCGTCAATGAGGTCCACGCTGCGGATT
ATGGCGATGTGCAGCTGTCCCACATGTATGCCGACAACGGCGCGATGCAGCTGGTGCGCGCCCCCAAGCAATTCGACGTG
ATCCTGACCGACAACCTCTTCGGCGATATCCTGAGCGACTGCGCCGCGATGCTGACCGGATCGTTGGGGATGCTGCCGTC
CGCAAGCCTGGGCGCACCGATGGCGAACGGCAGGCCCAAGGCGATGTATGAGCCCGTCCACGGCTCCGCGCCGGACATCA
CCGGGCAGGGCAAAGCGAACCCCATCGCCTGTATCCTCAGCTTCGCCATGGCGCTGCGCTACAGCTTCGATCTGGGCGAC
GAGGCCACGCGCCTTGAGGGCGCTGTCGAAAGCGTTCTTGCCGACGGTGCCCGCACGGCAGACCTGATGGGGCCGGATGG
CGGCACGCCGATCTCAACGTCACAGATGGGCGACGCCATTGTCGCCAAGCTGGCGGCGTGA

Upstream 100 bases:

>100_bases
CTGGACCGCGCTCGACACCGCAATCACCCCGCTGCTTTCTTGACGCCCTGCACCCCGCGCGCTACCGCAGGCGCAACCCA
ATTTCACGAGGACCAGACCC

Downstream 100 bases:

>100_bases
CCCTGAGCGTTCGGGCCTTCGCGGCGGAGGATGAGGCGGCTTTCCTCTCCCTCTATCGCGATTGCCTGGCGCATTACGGC
GTGGGCCCCTCCAGGCCCGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTDRSLLILPGDGIGPEVMAEVRKIIDWFGSARGISFDVSEDLVGGAAYDAHGVPLTDATMEKAQSVDAVLLGAVGGPAY
DDLDFSVKPERGLLRLRKEMDLYANLRPAQCFDALADFSSLKREIVSGLDIMILRELTSGVYFGEPRGIHTEGNERVGIN
TQRYTETEIARAAHSAFELARRRNNKVCSMEKANVMESGILWRDVVNEVHAADYGDVQLSHMYADNGAMQLVRAPKQFDV
ILTDNLFGDILSDCAAMLTGSLGMLPSASLGAPMANGRPKAMYEPVHGSAPDITGQGKANPIACILSFAMALRYSFDLGD
EATRLEGAVESVLADGARTADLMGPDGGTPISTSQMGDAIVAKLAA

Sequences:

>Translated_366_residues
MTDRSLLILPGDGIGPEVMAEVRKIIDWFGSARGISFDVSEDLVGGAAYDAHGVPLTDATMEKAQSVDAVLLGAVGGPAY
DDLDFSVKPERGLLRLRKEMDLYANLRPAQCFDALADFSSLKREIVSGLDIMILRELTSGVYFGEPRGIHTEGNERVGIN
TQRYTETEIARAAHSAFELARRRNNKVCSMEKANVMESGILWRDVVNEVHAADYGDVQLSHMYADNGAMQLVRAPKQFDV
ILTDNLFGDILSDCAAMLTGSLGMLPSASLGAPMANGRPKAMYEPVHGSAPDITGQGKANPIACILSFAMALRYSFDLGD
EATRLEGAVESVLADGARTADLMGPDGGTPISTSQMGDAIVAKLAA
>Mature_365_residues
TDRSLLILPGDGIGPEVMAEVRKIIDWFGSARGISFDVSEDLVGGAAYDAHGVPLTDATMEKAQSVDAVLLGAVGGPAYD
DLDFSVKPERGLLRLRKEMDLYANLRPAQCFDALADFSSLKREIVSGLDIMILRELTSGVYFGEPRGIHTEGNERVGINT
QRYTETEIARAAHSAFELARRRNNKVCSMEKANVMESGILWRDVVNEVHAADYGDVQLSHMYADNGAMQLVRAPKQFDVI
LTDNLFGDILSDCAAMLTGSLGMLPSASLGAPMANGRPKAMYEPVHGSAPDITGQGKANPIACILSFAMALRYSFDLGDE
ATRLEGAVESVLADGARTADLMGPDGGTPISTSQMGDAIVAKLAA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=344, Percent_Identity=35.4651162790698, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI28178816, Length=309, Percent_Identity=28.4789644012945, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI28178821, Length=309, Percent_Identity=28.4789644012945, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI4758582, Length=361, Percent_Identity=29.6398891966759, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI28178838, Length=329, Percent_Identity=30.6990881458967, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI28178819, Length=152, Percent_Identity=34.8684210526316, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=50.1377410468319, Blast_Score=338, Evalue=5e-94,
Organism=Escherichia coli, GI1788101, Length=370, Percent_Identity=32.972972972973, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787381, Length=409, Percent_Identity=25.9168704156479, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=362, Percent_Identity=35.6353591160221, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17550882, Length=363, Percent_Identity=30.5785123966942, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=317, Percent_Identity=29.9684542586751, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25144293, Length=361, Percent_Identity=27.7008310249307, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=362, Percent_Identity=42.2651933701657, Blast_Score=273, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6322097, Length=368, Percent_Identity=33.4239130434783, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6324291, Length=373, Percent_Identity=30.8310991957105, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324709, Length=369, Percent_Identity=32.520325203252, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24643270, Length=369, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24643268, Length=369, Percent_Identity=33.8753387533875, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24661184, Length=377, Percent_Identity=30.7692307692308, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI161078637, Length=362, Percent_Identity=27.6243093922652, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI161078635, Length=362, Percent_Identity=27.6243093922652, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI161078633, Length=362, Percent_Identity=27.6243093922652, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24650122, Length=362, Percent_Identity=27.6243093922652, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI161078639, Length=358, Percent_Identity=27.9329608938547, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281362242, Length=347, Percent_Identity=25.6484149855908, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24648872, Length=347, Percent_Identity=25.6484149855908, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_JANSC (Q28W67)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_508070.1
- ProteinModelPortal:   Q28W67
- SMR:   Q28W67
- STRING:   Q28W67
- GeneID:   3932564
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0128
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   AMQLVKW
- PhylomeDB:   Q28W67
- ProtClustDB:   PRK00772
- BioCyc:   JSP290400:JANN_0128-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 39119; Mature: 38988

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 97-97 BINDING 107-107 BINDING 135-135 BINDING 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRSLLILPGDGIGPEVMAEVRKIIDWFGSARGISFDVSEDLVGGAAYDAHGVPLTDAT
CCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEECCHHHCCCCCCCCCCCCCCHHH
MEKAQSVDAVLLGAVGGPAYDDLDFSVKPERGLLRLRKEMDLYANLRPAQCFDALADFSS
HHHHHHHCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LKREIVSGLDIMILRELTSGVYFGEPRGIHTEGNERVGINTQRYTETEIARAAHSAFELA
HHHHHHCCCHHHHHHHHHCCEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHH
RRRNNKVCSMEKANVMESGILWRDVVNEVHAADYGDVQLSHMYADNGAMQLVRAPKQFDV
HHCCCCEECCHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCEEE
ILTDNLFGDILSDCAAMLTGSLGMLPSASLGAPMANGRPKAMYEPVHGSAPDITGQGKAN
EEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCC
PIACILSFAMALRYSFDLGDEATRLEGAVESVLADGARTADLMGPDGGTPISTSQMGDAI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
VAKLAA
HHHHCC
>Mature Secondary Structure 
TDRSLLILPGDGIGPEVMAEVRKIIDWFGSARGISFDVSEDLVGGAAYDAHGVPLTDAT
CCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEECCHHHCCCCCCCCCCCCCCHHH
MEKAQSVDAVLLGAVGGPAYDDLDFSVKPERGLLRLRKEMDLYANLRPAQCFDALADFSS
HHHHHHHCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LKREIVSGLDIMILRELTSGVYFGEPRGIHTEGNERVGINTQRYTETEIARAAHSAFELA
HHHHHHCCCHHHHHHHHHCCEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHH
RRRNNKVCSMEKANVMESGILWRDVVNEVHAADYGDVQLSHMYADNGAMQLVRAPKQFDV
HHCCCCEECCHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCEEE
ILTDNLFGDILSDCAAMLTGSLGMLPSASLGAPMANGRPKAMYEPVHGSAPDITGQGKAN
EEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCC
PIACILSFAMALRYSFDLGDEATRLEGAVESVLADGARTADLMGPDGGTPISTSQMGDAI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
VAKLAA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA