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The map label for this gene is cca [H]

Identifier: 89052572

GI number: 89052572

Start: 74838

End: 75977

Strand: Direct

Name: cca [H]

Synonym: Jann_0081

Alternate gene names: 89052572

Gene position: 74838-75977 (Clockwise)

Preceding gene: 89052571

Following gene: 89052573

Centisome position: 1.73

GC content: 65.44

Gene sequence:

>1140_bases
ATGCGGCTGGACGCGGACTGGCTACGCAGGCCCGAGACCGGCGCTGTCTTCGATGCGCTGGACGGGCATGGCGTCTGGTT
CGTCGGTGGCTGCGTGCGCAATGGGCTGCTGGGGCTGGACGTGGCGGATATCGACATCTGCACCAATCTTCGGCCCGAAC
GGGTGATGGCGTTGGCGGAGGCCGCTGGATTGAAGGCGGTGCCCACAGGCATCGACCACGGAACGGTGACTGTCGTATCC
GGGGGCCTTCCTTACGAAATTACAACCCTGCGCCGCGATGTAGAGACCGATGGCCGCCATGCGTCTGTCGCTTTCACCGA
CGTATTGGCGGAGGATGCGGCGCGGCGCGATTTCACCATGAACGCGCTCTATGCGACCCGAGGCGGGGAGGTTCTGGACC
CTAACGGGGACGGCCTGTCGGACCTGCACGCGCGGCGGGTGCGGTTTATCGGGGATGCCGATGCGCGGATTGCCGAGGAT
TACCTGCGCATCCTGCGCTTTTTTCGATTCCATGCCTGGTACGCCGACCCTTCGGGTGGGCTGGATGCCGAGAGCCTTGC
CGCCTGCGCGGCTGGATTAGACGGGATCGCGCAGCTATCGCGGGAGCGGGTTGGGGCGGAGATCAAAACGCTCCTCCGCG
CGCCTGATCCCGCGCCCGCTGTCGCGGCGATGGATCAGACGGGCGTCCTCGCGGCGGTTCTGCCCGGCGCGTCGTCCCGG
ATGCTGACGCTTCTGACTGGGCTGGAGACCGGGCTGACACCGGACGCGGTGCGTCGTCTTGCGGCTCTGGGCGGGGACGA
TGTGGCCGAGCGACTGCGCCTGTCCCGGTCCGATAGCAAACGGCTGACGCTCTATCGGACTGAGATGGGCGCGATGACCA
CCCCCGGTGCCCTGGGCTATCACCACGGCCTGGACGCCGCGCGTGATATTCTGGTTCTACGCGGCCTGCTCTCCGAGCAA
CCTGTGACGGCAAGCGCGCTGCACACAGCAAAACTTGGTGCAACAGCAACCTTCCCGATCAGGGCCGCTGATCTGCCAGA
CCTGCACGGCAAACCCCTTGGCGACCGTTTGAAATTGCTGGAGGCGCGCTGGATCGACAGCACGTTCACGCTGACAAAGT
CAGAGCTTCTCAACCGGTAA

Upstream 100 bases:

>100_bases
GCGGCATGCGGCGGGAATATTACGTCGTCCCGTTCGGCCCCTATTACATCTGGGGCGCGACGGCGCGAATACTGAAATCC
CTCGCGGATCGGGTGGTGTG

Downstream 100 bases:

>100_bases
TGGTCATTCCCGTCGCGGCGCGCTATACGGGCCGCCTGACACGGGAAATGTCATGTTCAGAAAATTTGAAAATCTGGTCG
ATCCCTATGTGGCGCATGCC

Product: polynucleotide adenylyltransferase protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MRLDADWLRRPETGAVFDALDGHGVWFVGGCVRNGLLGLDVADIDICTNLRPERVMALAEAAGLKAVPTGIDHGTVTVVS
GGLPYEITTLRRDVETDGRHASVAFTDVLAEDAARRDFTMNALYATRGGEVLDPNGDGLSDLHARRVRFIGDADARIAED
YLRILRFFRFHAWYADPSGGLDAESLAACAAGLDGIAQLSRERVGAEIKTLLRAPDPAPAVAAMDQTGVLAAVLPGASSR
MLTLLTGLETGLTPDAVRRLAALGGDDVAERLRLSRSDSKRLTLYRTEMGAMTTPGALGYHHGLDAARDILVLRGLLSEQ
PVTASALHTAKLGATATFPIRAADLPDLHGKPLGDRLKLLEARWIDSTFTLTKSELLNR

Sequences:

>Translated_379_residues
MRLDADWLRRPETGAVFDALDGHGVWFVGGCVRNGLLGLDVADIDICTNLRPERVMALAEAAGLKAVPTGIDHGTVTVVS
GGLPYEITTLRRDVETDGRHASVAFTDVLAEDAARRDFTMNALYATRGGEVLDPNGDGLSDLHARRVRFIGDADARIAED
YLRILRFFRFHAWYADPSGGLDAESLAACAAGLDGIAQLSRERVGAEIKTLLRAPDPAPAVAAMDQTGVLAAVLPGASSR
MLTLLTGLETGLTPDAVRRLAALGGDDVAERLRLSRSDSKRLTLYRTEMGAMTTPGALGYHHGLDAARDILVLRGLLSEQ
PVTASALHTAKLGATATFPIRAADLPDLHGKPLGDRLKLLEARWIDSTFTLTKSELLNR
>Mature_379_residues
MRLDADWLRRPETGAVFDALDGHGVWFVGGCVRNGLLGLDVADIDICTNLRPERVMALAEAAGLKAVPTGIDHGTVTVVS
GGLPYEITTLRRDVETDGRHASVAFTDVLAEDAARRDFTMNALYATRGGEVLDPNGDGLSDLHARRVRFIGDADARIAED
YLRILRFFRFHAWYADPSGGLDAESLAACAAGLDGIAQLSRERVGAEIKTLLRAPDPAPAVAAMDQTGVLAAVLPGASSR
MLTLLTGLETGLTPDAVRRLAALGGDDVAERLRLSRSDSKRLTLYRTEMGAMTTPGALGYHHGLDAARDILVLRGLLSEQ
PVTASALHTAKLGATATFPIRAADLPDLHGKPLGDRLKLLEARWIDSTFTLTKSELLNR

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=381, Percent_Identity=31.758530183727, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI87081691, Length=163, Percent_Identity=32.5153374233129, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789436, Length=216, Percent_Identity=31.4814814814815, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71995920, Length=378, Percent_Identity=29.6296296296296, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI281360112, Length=391, Percent_Identity=31.7135549872123, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21357337, Length=391, Percent_Identity=31.7135549872123, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 40577; Mature: 40577

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDADWLRRPETGAVFDALDGHGVWFVGGCVRNGLLGLDVADIDICTNLRPERVMALAE
CCCCHHHHCCCCCCCEEECCCCCCEEEEEHHHHCCCCCCEEEHHHHHCCCCHHHHHHHHH
AAGLKAVPTGIDHGTVTVVSGGLPYEITTLRRDVETDGRHASVAFTDVLAEDAARRDFTM
HCCCEECCCCCCCCEEEEEECCCCEEEHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCEE
NALYATRGGEVLDPNGDGLSDLHARRVRFIGDADARIAEDYLRILRFFRFHAWYADPSGG
EEEEEECCCCEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCC
LDAESLAACAAGLDGIAQLSRERVGAEIKTLLRAPDPAPAVAAMDQTGVLAAVLPGASSR
CCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCCCCH
MLTLLTGLETGLTPDAVRRLAALGGDDVAERLRLSRSDSKRLTLYRTEMGAMTTPGALGY
HHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCH
HHGLDAARDILVLRGLLSEQPVTASALHTAKLGATATFPIRAADLPDLHGKPLGDRLKLL
HCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCEEECCCCCCCCCCCHHHHHHH
EARWIDSTFTLTKSELLNR
HHHHCCCHHEEEHHHHCCC
>Mature Secondary Structure
MRLDADWLRRPETGAVFDALDGHGVWFVGGCVRNGLLGLDVADIDICTNLRPERVMALAE
CCCCHHHHCCCCCCCEEECCCCCCEEEEEHHHHCCCCCCEEEHHHHHCCCCHHHHHHHHH
AAGLKAVPTGIDHGTVTVVSGGLPYEITTLRRDVETDGRHASVAFTDVLAEDAARRDFTM
HCCCEECCCCCCCCEEEEEECCCCEEEHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCEE
NALYATRGGEVLDPNGDGLSDLHARRVRFIGDADARIAEDYLRILRFFRFHAWYADPSGG
EEEEEECCCCEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCC
LDAESLAACAAGLDGIAQLSRERVGAEIKTLLRAPDPAPAVAAMDQTGVLAAVLPGASSR
CCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCCCCH
MLTLLTGLETGLTPDAVRRLAALGGDDVAERLRLSRSDSKRLTLYRTEMGAMTTPGALGY
HHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCH
HHGLDAARDILVLRGLLSEQPVTASALHTAKLGATATFPIRAADLPDLHGKPLGDRLKLL
HCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEECCEEECCCCCCCCCCCHHHHHHH
EARWIDSTFTLTKSELLNR
HHHHCCCHHEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA