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The map label for this gene is nusA

Identifier: 89052531

GI number: 89052531

Start: 38314

End: 40086

Strand: Reverse

Name: nusA

Synonym: Jann_0040

Alternate gene names: 89052531

Gene position: 40086-38314 (Counterclockwise)

Preceding gene: 89052532

Following gene: 89052530

Centisome position: 0.93

GC content: 60.12

Gene sequence:

>1773_bases
ATGGCCATCACGTCAGCCAACCAGCTGGAGCTTTTGCAAACCGCAGAAGCCGTTGCCCGCGAAAAGATGATCGACCCCAA
TCTGGTGGTCGAGGCGATGGAAGAAAGTCTCGCCCGGGCTGCAAAGTCCCGCTATGGGTCCGAGCTGGATATCCGCGTTT
CCATCGACCGCAAGACGGGCCGCGCCACGTTCACCCGTGTGCGCACCGTGGCGGATGAAGACACGCTGGAAAACGACAAG
GCCGAAATGCTGCTGGCCGATGCCGACGCAACGGTTGCCGCCGAGCGCCCCAATGTCGTGGTCTTCAACGCCAAGTCCCA
CGTCTATGATGAGGAAGGCGAGGTTGTGGAGACCCGCGACGTGCGCCGTTTTGTCATGCGCAAGCCGACCGAGAATGACA
AGCTCCTGACCGAGGGCGTGGATCAATCCACCGGCCCCGTGATCGGTGACATGATCGCCGACGAAGTGCCGCCCGTGGAA
ATGGGCCGGATCGCCGCGCAATCGGCCAAGCAGGTCATTTTGCAGAAGGTCCGCGAAGCCGAGCGTGACCGTCAGTTCGC
CGAGTTCAAGGACCGTGTGGGCGAGATCATCAACGGCGTCGTCAAGCGTGAGGAATACGGCAACGTCATCGTCGACGTAG
GCTCCGGTGAGGCGCAGCTGCGCCGCAACGAGAAGATCGGGCGGGAAGCCTATCGCAACGGCGACCGCATTCGCTGCTAC
ATCAAGGATGTGCGCCGTGAGAACCGCGGCCATCAGATCTTCCTGTCGCGCACCGCGCCGGAGTTCATGCGCGAGTTGTT
CAAGATGGAAGTGCCCGAGATCTATGACGGCATCATCGAGATCAAGGCCGTCGCCCGCGATCCCGGGTCGCGTGCCAAGA
TTGCGGTGATTTCCTATGACAATGGCATCGACCCCGTGGGCGCCTGTGTGGGTATGCGCGGCAGCCGGGTGCAGGCTGTC
GTCAACGAATTGCAGGGCGAAAAGATCGACATCATTCCGTGGAATGAGGATGCGCCGACGTTCCTGGTGAATGCGCTGCA
ACCTGCCGAGGTCTCCAAGGTGGTTCTGGATGAGGACGCCGGGAAGATCGAAGTCGTGGTGCCCGATGAGCAGCTGAGCC
TTGCCATTGGTCGGCGCGGTCAGAACGTGCGTCTTGCCTCACAGCTGACCAACCTTGATATCGATATCCTGACCGAGGAA
GAGGAATCCAAGCGTCGTCAGGCCGAGTTTGAAGAGCGCACGAAGCTGTTCATGGATACGCTCGACCTGGATGAGTTCTT
CGCGCAGCTTCTGGTCTCCGAAGGCTTCACGGCGTTGGAAGAAGTGGCCTATGTGGAAGCGGACGAGCTTCTGGTCATCG
ACGGCGTTGATGAGGGTACTGCGGAAGAATTGCAGGCCCGCGCCCGCGATTATTTGGAGGCGCAGAATAAACTGGCGCTT
GAGAAGGCGAAAGAGATGGGCGTTGAAGAGAGCCTCATTGCATTTGAGGGCCTTACTCCCCAAATGTTGGTGGCCTTGGG
CGAAGACGGCGTGAAAACGCTGGAAGACTTCGCTACCTGCGCAGATTGGGAACTGGCCGGCGGTTGGACAACCGAAGGCG
GAGAGCGCATCAAGGACGACGGCCTTTTGGAGCCCTTCGAAGTCTCTTTGGAAGAAGCACAGAACATGGTGATGACCGCA
CGCCTGCAACTGGGTTGGGTGACGATCGAGGAATTGGAAGCCGACGCCGCTGCCGAAGCAGAAGCCGCCGCACAGGAGGG
GGCAGAGGGCTAA

Upstream 100 bases:

>100_bases
ATCAAGGAAATGCTGAAGCAACGCAAGGACGCGGGGCTGATCAACGAGACACAATTTGACGACATCGAGGAAGATCCCTC
GAACCCGGAGGACTAAGCAT

Downstream 100 bases:

>100_bases
GGCCCTCGGCCCGGATCCGCGAATGTCTCGTGGTGGCCAACATAAGGACCGAAGCGACGGGCCTACGCGCAAGTGCATTG
CTACCGGTGAGGTCGCGCCC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 590; Mature: 589

Protein sequence:

>590_residues
MAITSANQLELLQTAEAVAREKMIDPNLVVEAMEESLARAAKSRYGSELDIRVSIDRKTGRATFTRVRTVADEDTLENDK
AEMLLADADATVAAERPNVVVFNAKSHVYDEEGEVVETRDVRRFVMRKPTENDKLLTEGVDQSTGPVIGDMIADEVPPVE
MGRIAAQSAKQVILQKVREAERDRQFAEFKDRVGEIINGVVKREEYGNVIVDVGSGEAQLRRNEKIGREAYRNGDRIRCY
IKDVRRENRGHQIFLSRTAPEFMRELFKMEVPEIYDGIIEIKAVARDPGSRAKIAVISYDNGIDPVGACVGMRGSRVQAV
VNELQGEKIDIIPWNEDAPTFLVNALQPAEVSKVVLDEDAGKIEVVVPDEQLSLAIGRRGQNVRLASQLTNLDIDILTEE
EESKRRQAEFEERTKLFMDTLDLDEFFAQLLVSEGFTALEEVAYVEADELLVIDGVDEGTAEELQARARDYLEAQNKLAL
EKAKEMGVEESLIAFEGLTPQMLVALGEDGVKTLEDFATCADWELAGGWTTEGGERIKDDGLLEPFEVSLEEAQNMVMTA
RLQLGWVTIEELEADAAAEAEAAAQEGAEG

Sequences:

>Translated_590_residues
MAITSANQLELLQTAEAVAREKMIDPNLVVEAMEESLARAAKSRYGSELDIRVSIDRKTGRATFTRVRTVADEDTLENDK
AEMLLADADATVAAERPNVVVFNAKSHVYDEEGEVVETRDVRRFVMRKPTENDKLLTEGVDQSTGPVIGDMIADEVPPVE
MGRIAAQSAKQVILQKVREAERDRQFAEFKDRVGEIINGVVKREEYGNVIVDVGSGEAQLRRNEKIGREAYRNGDRIRCY
IKDVRRENRGHQIFLSRTAPEFMRELFKMEVPEIYDGIIEIKAVARDPGSRAKIAVISYDNGIDPVGACVGMRGSRVQAV
VNELQGEKIDIIPWNEDAPTFLVNALQPAEVSKVVLDEDAGKIEVVVPDEQLSLAIGRRGQNVRLASQLTNLDIDILTEE
EESKRRQAEFEERTKLFMDTLDLDEFFAQLLVSEGFTALEEVAYVEADELLVIDGVDEGTAEELQARARDYLEAQNKLAL
EKAKEMGVEESLIAFEGLTPQMLVALGEDGVKTLEDFATCADWELAGGWTTEGGERIKDDGLLEPFEVSLEEAQNMVMTA
RLQLGWVTIEELEADAAAEAEAAAQEGAEG
>Mature_589_residues
AITSANQLELLQTAEAVAREKMIDPNLVVEAMEESLARAAKSRYGSELDIRVSIDRKTGRATFTRVRTVADEDTLENDKA
EMLLADADATVAAERPNVVVFNAKSHVYDEEGEVVETRDVRRFVMRKPTENDKLLTEGVDQSTGPVIGDMIADEVPPVEM
GRIAAQSAKQVILQKVREAERDRQFAEFKDRVGEIINGVVKREEYGNVIVDVGSGEAQLRRNEKIGREAYRNGDRIRCYI
KDVRRENRGHQIFLSRTAPEFMRELFKMEVPEIYDGIIEIKAVARDPGSRAKIAVISYDNGIDPVGACVGMRGSRVQAVV
NELQGEKIDIIPWNEDAPTFLVNALQPAEVSKVVLDEDAGKIEVVVPDEQLSLAIGRRGQNVRLASQLTNLDIDILTEEE
ESKRRQAEFEERTKLFMDTLDLDEFFAQLLVSEGFTALEEVAYVEADELLVIDGVDEGTAEELQARARDYLEAQNKLALE
KAKEMGVEESLIAFEGLTPQMLVALGEDGVKTLEDFATCADWELAGGWTTEGGERIKDDGLLEPFEVSLEEAQNMVMTAR
LQLGWVTIEELEADAAAEAEAAAQEGAEG

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=372, Percent_Identity=47.3118279569892, Blast_Score=327, Evalue=1e-90,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 65484; Mature: 65353

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITSANQLELLQTAEAVAREKMIDPNLVVEAMEESLARAAKSRYGSELDIRVSIDRKTG
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
RATFTRVRTVADEDTLENDKAEMLLADADATVAAERPNVVVFNAKSHVYDEEGEVVETRD
CHHHHHHHHHHCCHHCCCCCHHEEEECCCCEEEECCCCEEEECCCCCCCCCCCCEEHHHH
VRRFVMRKPTENDKLLTEGVDQSTGPVIGDMIADEVPPVEMGRIAAQSAKQVILQKVREA
HHHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ERDRQFAEFKDRVGEIINGVVKREEYGNVIVDVGSGEAQLRRNEKIGREAYRNGDRIRCY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEE
IKDVRRENRGHQIFLSRTAPEFMRELFKMEVPEIYDGIIEIKAVARDPGSRAKIAVISYD
EHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHEEEEECCCCCCCCEEEEEEEC
NGIDPVGACVGMRGSRVQAVVNELQGEKIDIIPWNEDAPTFLVNALQPAEVSKVVLDEDA
CCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCCCCCHHHHEEEECCC
GKIEVVVPDEQLSLAIGRRGQNVRLASQLTNLDIDILTEEEESKRRQAEFEERTKLFMDT
CEEEEEECCHHHHHHCCCCCCCEEHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
LDLDEFFAQLLVSEGFTALEEVAYVEADELLVIDGVDEGTAEELQARARDYLEAQNKLAL
CCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
EKAKEMGVEESLIAFEGLTPQMLVALGEDGVKTLEDFATCADWELAGGWTTEGGERIKDD
HHHHHCCCHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCCEECCCEECCCCCCCCCC
GLLEPFEVSLEEAQNMVMTARLQLGWVTIEELEADAAAEAEAAAQEGAEG
CCCCHHHHHHHHHHCCEEEEEEEECEEEHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AITSANQLELLQTAEAVAREKMIDPNLVVEAMEESLARAAKSRYGSELDIRVSIDRKTG
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
RATFTRVRTVADEDTLENDKAEMLLADADATVAAERPNVVVFNAKSHVYDEEGEVVETRD
CHHHHHHHHHHCCHHCCCCCHHEEEECCCCEEEECCCCEEEECCCCCCCCCCCCEEHHHH
VRRFVMRKPTENDKLLTEGVDQSTGPVIGDMIADEVPPVEMGRIAAQSAKQVILQKVREA
HHHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
ERDRQFAEFKDRVGEIINGVVKREEYGNVIVDVGSGEAQLRRNEKIGREAYRNGDRIRCY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEE
IKDVRRENRGHQIFLSRTAPEFMRELFKMEVPEIYDGIIEIKAVARDPGSRAKIAVISYD
EHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHEEEEECCCCCCCCEEEEEEEC
NGIDPVGACVGMRGSRVQAVVNELQGEKIDIIPWNEDAPTFLVNALQPAEVSKVVLDEDA
CCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCCCCCHHHHEEEECCC
GKIEVVVPDEQLSLAIGRRGQNVRLASQLTNLDIDILTEEEESKRRQAEFEERTKLFMDT
CEEEEEECCHHHHHHCCCCCCCEEHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
LDLDEFFAQLLVSEGFTALEEVAYVEADELLVIDGVDEGTAEELQARARDYLEAQNKLAL
CCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
EKAKEMGVEESLIAFEGLTPQMLVALGEDGVKTLEDFATCADWELAGGWTTEGGERIKDD
HHHHHCCCHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCCEECCCEECCCCCCCCCC
GLLEPFEVSLEEAQNMVMTARLQLGWVTIEELEADAAAEAEAAAQEGAEG
CCCCHHHHHHHHHHCCEEEEEEEECEEEHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]