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The map label for this gene is sseA [H]

Identifier: 89052521

GI number: 89052521

Start: 27505

End: 28368

Strand: Reverse

Name: sseA [H]

Synonym: Jann_0030

Alternate gene names: 89052521

Gene position: 28368-27505 (Counterclockwise)

Preceding gene: 89052522

Following gene: 89052520

Centisome position: 0.66

GC content: 61.69

Gene sequence:

>864_bases
ATGGCCGAGGTGATCACCGGGGACGATCCGCAAACGCTGGTCTCGACTGATTGGCTCGCCGCGCATTTCAACGATCCTGA
TCTGCGGATCATCGACGCATCGTATTATCTGGCCGAGATGAACCGGGATGCGAAGGCCGAATATGACGCGGGCCACATCC
CCGGCGCGCGGTTTTTCGACATTGATGACATCTCCGACGCGCGATCCGAGCTGCCGCATATGGTGGCCCCGGTGGAGAAG
TTCATGTCCCGCATGCGCGCCATGGGCGTGGGCGATGGTCATCAGGTGGTGGTCTATGACGGACGTGGCGTGTTTTCTGC
CGCGCGTGTTTGGTGGAACTTTCGTCTGATGGGCAAGACAGATGTCGCGGTCCTGGATGGAGGGCTGCCCAAATGGGTCG
CCGAAGGGCGTCCCGTAGAAGATATGCCGCCGATCATCCGGGACCGTCACATGACGGTGCAGCGTCAGGCGCATCTGGTG
AAGGATGTCACGCAGGTGGCCTCGGCCAGCAAGCTTGGCGATTGGCAGATCGTGGACGCCCGCGCGCCCGCAAGGTTCCG
CGGAGAGGAGCCCGAGACGCGACCCGGCCTGCGGGCTGGCCGTATCCCGAATTCCAAGAACGTCCATTACGCCAGTCTGT
TCAAACCCGATGGCACGATGAAAGAAGGCGACGCCCTGCGCGCCGCGTTCGAGGCTGGCGGCGTGGATCTGGACAAGCGG
ATCATCACGACTTGTGGATCCGGCGTGACGGCAGCGATCCTGATGCTGGGGCTGACGCGTTTGGGCCATCAGGATGTGTC
GCTCTATGATGGATCATGGTCCGAATGGGGCCAGTTTGAGCAGTTGAAGGTCGAAACCGGATGA

Upstream 100 bases:

>100_bases
GTATTTACCAAGATGAAGGAGGAGCGTCGCGCGGGCGAGGCTTGCGTGGCTCTGGGCGGCGGCGTAGGACGTTTGGGGTA
TTTGGACGGGAGTGCGCAGA

Downstream 100 bases:

>100_bases
GCGTGATCCCCGCGGGCACGCAGGTCCCCTTCACGATCACCTATCTGGAAATGACCGCGCGACCCGATTTTGGCCCTGCG
CAACTGCCCGGTGACGTGCG

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MAEVITGDDPQTLVSTDWLAAHFNDPDLRIIDASYYLAEMNRDAKAEYDAGHIPGARFFDIDDISDARSELPHMVAPVEK
FMSRMRAMGVGDGHQVVVYDGRGVFSAARVWWNFRLMGKTDVAVLDGGLPKWVAEGRPVEDMPPIIRDRHMTVQRQAHLV
KDVTQVASASKLGDWQIVDARAPARFRGEEPETRPGLRAGRIPNSKNVHYASLFKPDGTMKEGDALRAAFEAGGVDLDKR
IITTCGSGVTAAILMLGLTRLGHQDVSLYDGSWSEWGQFEQLKVETG

Sequences:

>Translated_287_residues
MAEVITGDDPQTLVSTDWLAAHFNDPDLRIIDASYYLAEMNRDAKAEYDAGHIPGARFFDIDDISDARSELPHMVAPVEK
FMSRMRAMGVGDGHQVVVYDGRGVFSAARVWWNFRLMGKTDVAVLDGGLPKWVAEGRPVEDMPPIIRDRHMTVQRQAHLV
KDVTQVASASKLGDWQIVDARAPARFRGEEPETRPGLRAGRIPNSKNVHYASLFKPDGTMKEGDALRAAFEAGGVDLDKR
IITTCGSGVTAAILMLGLTRLGHQDVSLYDGSWSEWGQFEQLKVETG
>Mature_286_residues
AEVITGDDPQTLVSTDWLAAHFNDPDLRIIDASYYLAEMNRDAKAEYDAGHIPGARFFDIDDISDARSELPHMVAPVEKF
MSRMRAMGVGDGHQVVVYDGRGVFSAARVWWNFRLMGKTDVAVLDGGLPKWVAEGRPVEDMPPIIRDRHMTVQRQAHLVK
DVTQVASASKLGDWQIVDARAPARFRGEEPETRPGLRAGRIPNSKNVHYASLFKPDGTMKEGDALRAAFEAGGVDLDKRI
ITTCGSGVTAAILMLGLTRLGHQDVSLYDGSWSEWGQFEQLKVETG

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=272, Percent_Identity=38.9705882352941, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI61835204, Length=272, Percent_Identity=38.9705882352941, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI194473681, Length=272, Percent_Identity=38.9705882352941, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI17402865, Length=273, Percent_Identity=38.8278388278388, Blast_Score=179, Evalue=2e-45,
Organism=Escherichia coli, GI87082121, Length=274, Percent_Identity=43.4306569343066, Blast_Score=210, Evalue=8e-56,
Organism=Escherichia coli, GI87081967, Length=273, Percent_Identity=28.2051282051282, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17561888, Length=290, Percent_Identity=27.2413793103448, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71997283, Length=291, Percent_Identity=25.4295532646048, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17543836, Length=288, Percent_Identity=26.3888888888889, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17543838, Length=288, Percent_Identity=26.3888888888889, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI115534702, Length=233, Percent_Identity=27.4678111587983, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324825, Length=264, Percent_Identity=31.8181818181818, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 31709; Mature: 31578

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVITGDDPQTLVSTDWLAAHFNDPDLRIIDASYYLAEMNRDAKAEYDAGHIPGARFFD
CCCCCCCCCCCCEEECCEEEEECCCCCEEEEECHHHEECCCCCCCCCCCCCCCCCCEEEE
IDDISDARSELPHMVAPVEKFMSRMRAMGVGDGHQVVVYDGRGVFSAARVWWNFRLMGKT
CCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHEEEEEEEEECCC
DVAVLDGGLPKWVAEGRPVEDMPPIIRDRHMTVQRQAHLVKDVTQVASASKLGDWQIVDA
CEEEECCCCHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
RAPARFRGEEPETRPGLRAGRIPNSKNVHYASLFKPDGTMKEGDALRAAFEAGGVDLDKR
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCHHHH
IITTCGSGVTAAILMLGLTRLGHQDVSLYDGSWSEWGQFEQLKVETG
HHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHCCCEEEEEEECC
>Mature Secondary Structure 
AEVITGDDPQTLVSTDWLAAHFNDPDLRIIDASYYLAEMNRDAKAEYDAGHIPGARFFD
CCCCCCCCCCCEEECCEEEEECCCCCEEEEECHHHEECCCCCCCCCCCCCCCCCCEEEE
IDDISDARSELPHMVAPVEKFMSRMRAMGVGDGHQVVVYDGRGVFSAARVWWNFRLMGKT
CCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHEEEEEEEEECCC
DVAVLDGGLPKWVAEGRPVEDMPPIIRDRHMTVQRQAHLVKDVTQVASASKLGDWQIVDA
CEEEECCCCHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
RAPARFRGEEPETRPGLRAGRIPNSKNVHYASLFKPDGTMKEGDALRAAFEAGGVDLDKR
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCCHHHH
IITTCGSGVTAAILMLGLTRLGHQDVSLYDGSWSEWGQFEQLKVETG
HHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]