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The map label for this gene is smpB

Identifier: 89052522

GI number: 89052522

Start: 28499

End: 28975

Strand: Reverse

Name: smpB

Synonym: Jann_0031

Alternate gene names: 89052522

Gene position: 28975-28499 (Counterclockwise)

Preceding gene: 89052523

Following gene: 89052521

Centisome position: 0.67

GC content: 58.07

Gene sequence:

>477_bases
ATGGCCAAGAAACCTGATAATCCAAACTACAAAGTCATCGCCGAGAACCGGCGGGCCCGCTTCGATTACGCCATCGAAAG
CGATCTTGAGGTTGGGATCATCCTGGAAGGGTCCGAGGTCAAGAGCCTGCGGCAGGGACAAAGCAATATCGCCGAAAGCT
ATGCCGAGGTGAAGGAGGGTGAGCTGTGGCTCGTGAACTCCTACATCGCGCCTTATGTGCAGGCCAAGACGTTCGGGCAC
GAGGAAAAGCGGCGGCGCAAGATGCTGGTGTCGCGCAAGGAGTTGTCGAAGCTGTGGAACGCCACCCAGCGTGAGGGCAT
GACGCTGGTGCCGATTGTCATGTACTTCAATCACAAGGGTCTGGTGAAGCTGAAGATCGGGATCGCCAAGGGCAAGAAGG
CGCAGGACAAGCGCGAAACCTCGGCCAAGCGCGACTGGAACCGTCAGAAGGCGCGGCTGCTCAAGCAAAACGGCTGA

Upstream 100 bases:

>100_bases
GCGCTATGGCGCTTGTGGGTGTGGCGCACCTGTTGGTGTCGGCCCGGATCAGCCGCTAGGCGTAGACACCTGTGGGCGGA
GTGCCTAGATACACCGGACC

Downstream 100 bases:

>100_bases
CAGTGCCCAGGGCAGTCGGCGGATTGAGTTGTATTTACCAAGATGAAGGAGGAGCGTCGCGCGGGCGAGGCTTGCGTGGC
TCTGGGCGGCGGCGTAGGAC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MAKKPDNPNYKVIAENRRARFDYAIESDLEVGIILEGSEVKSLRQGQSNIAESYAEVKEGELWLVNSYIAPYVQAKTFGH
EEKRRRKMLVSRKELSKLWNATQREGMTLVPIVMYFNHKGLVKLKIGIAKGKKAQDKRETSAKRDWNRQKARLLKQNG

Sequences:

>Translated_158_residues
MAKKPDNPNYKVIAENRRARFDYAIESDLEVGIILEGSEVKSLRQGQSNIAESYAEVKEGELWLVNSYIAPYVQAKTFGH
EEKRRRKMLVSRKELSKLWNATQREGMTLVPIVMYFNHKGLVKLKIGIAKGKKAQDKRETSAKRDWNRQKARLLKQNG
>Mature_157_residues
AKKPDNPNYKVIAENRRARFDYAIESDLEVGIILEGSEVKSLRQGQSNIAESYAEVKEGELWLVNSYIAPYVQAKTFGHE
EKRRRKMLVSRKELSKLWNATQREGMTLVPIVMYFNHKGLVKLKIGIAKGKKAQDKRETSAKRDWNRQKARLLKQNG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=154, Percent_Identity=42.2077922077922, Blast_Score=120, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_JANSC (Q28WG4)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_507973.1
- ProteinModelPortal:   Q28WG4
- SMR:   Q28WG4
- STRING:   Q28WG4
- GeneID:   3932467
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_0031
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   Q28WG4
- ProtClustDB:   PRK05422
- BioCyc:   JSP290400:JANN_0031-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18249; Mature: 18118

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKPDNPNYKVIAENRRARFDYAIESDLEVGIILEGSEVKSLRQGQSNIAESYAEVKEG
CCCCCCCCCEEEEECCCCCEECEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCC
ELWLVNSYIAPYVQAKTFGHEEKRRRKMLVSRKELSKLWNATQREGMTLVPIVMYFNHKG
CEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCC
LVKLKIGIAKGKKAQDKRETSAKRDWNRQKARLLKQNG
EEEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure 
AKKPDNPNYKVIAENRRARFDYAIESDLEVGIILEGSEVKSLRQGQSNIAESYAEVKEG
CCCCCCCCEEEEECCCCCEECEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCC
ELWLVNSYIAPYVQAKTFGHEEKRRRKMLVSRKELSKLWNATQREGMTLVPIVMYFNHKG
CEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCC
LVKLKIGIAKGKKAQDKRETSAKRDWNRQKARLLKQNG
EEEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA