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The map label for this gene is pncA [H]

Identifier: 89052505

GI number: 89052505

Start: 13339

End: 13932

Strand: Direct

Name: pncA [H]

Synonym: Jann_0014

Alternate gene names: 89052505

Gene position: 13339-13932 (Clockwise)

Preceding gene: 89052501

Following gene: 89052507

Centisome position: 0.31

GC content: 62.12

Gene sequence:

>594_bases
ATGACCCAAGCCCTACTTGTTATCGACGTCCAGAACGATTTTTGCCCCGGCGGCGCACTGGCCGTGACCGATGGGGATAC
CATTGTGCCCGGCATCAACGCGCTGATGGCGGAGTTCAATGCCGTGATCCTGACCCAAGACTGGCACCCGGCGGGGCACA
GTTCTTTCGCGTCCAGCCATGACGCAGAACCGATGAGCCTGATGCAGATGCCCTACGGCCCCCAGGTCCTCTGGCCCGAT
CATTGCATCCAGGGCAGCGTCGGCGCGCAGTTCCACGCGGACCTGACAACCGACCGCGCCGATCTGATCATCCGCAAGGG
CTACAACCCCGCCATCGACAGCTATTCCGCCTTCTTTGAAAATGATCACACCACGCCCACCGGCCTTGAAGGGTATTTGC
GCACCCGGGGGATCACCGACCTGACGCTTGTGGGCCTCGCCACGGATTTCTGCGTGAACTACTCCGCCGTGGATGCCGCC
AAGCTGGGGTTCACCGTCACCATCCGCACGGACCTGTGCCGCGCGATTGATTTTGACGGGTCGCTGACAAACGCCCTGAC
CGCCATGCACGCGGCAGGCGCGACAATCGTCTAA

Upstream 100 bases:

>100_bases
TCATGGGCGTCAGTATTGCCGATTCGTGTCATGACAACAAGACAACAGATTGCACATGCGGGAAGAGAGCGTAGGCTGCC
GCAATCTACAGGAGGCCCAC

Downstream 100 bases:

>100_bases
CCCTCCGGCTGTGCAATGGGCAGCGGCTCCTTGAACGTCAGGGTGCGGTACGGGAACGGGATCTCGATCCCGGCATCATC
AAGTGCGCGTTTCACACCTG

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MTQALLVIDVQNDFCPGGALAVTDGDTIVPGINALMAEFNAVILTQDWHPAGHSSFASSHDAEPMSLMQMPYGPQVLWPD
HCIQGSVGAQFHADLTTDRADLIIRKGYNPAIDSYSAFFENDHTTPTGLEGYLRTRGITDLTLVGLATDFCVNYSAVDAA
KLGFTVTIRTDLCRAIDFDGSLTNALTAMHAAGATIV

Sequences:

>Translated_197_residues
MTQALLVIDVQNDFCPGGALAVTDGDTIVPGINALMAEFNAVILTQDWHPAGHSSFASSHDAEPMSLMQMPYGPQVLWPD
HCIQGSVGAQFHADLTTDRADLIIRKGYNPAIDSYSAFFENDHTTPTGLEGYLRTRGITDLTLVGLATDFCVNYSAVDAA
KLGFTVTIRTDLCRAIDFDGSLTNALTAMHAAGATIV
>Mature_196_residues
TQALLVIDVQNDFCPGGALAVTDGDTIVPGINALMAEFNAVILTQDWHPAGHSSFASSHDAEPMSLMQMPYGPQVLWPDH
CIQGSVGAQFHADLTTDRADLIIRKGYNPAIDSYSAFFENDHTTPTGLEGYLRTRGITDLTLVGLATDFCVNYSAVDAAK
LGFTVTIRTDLCRAIDFDGSLTNALTAMHAAGATIV

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=199, Percent_Identity=46.7336683417085, Blast_Score=183, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI193206735, Length=191, Percent_Identity=35.6020942408377, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17543130, Length=191, Percent_Identity=35.6020942408377, Blast_Score=111, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71999339, Length=193, Percent_Identity=34.1968911917098, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321401, Length=204, Percent_Identity=31.3725490196078, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648247, Length=199, Percent_Identity=36.1809045226131, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 21037; Mature: 20906

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQALLVIDVQNDFCPGGALAVTDGDTIVPGINALMAEFNAVILTQDWHPAGHSSFASSH
CCCEEEEEECCCCCCCCCEEEEECCCEECCCHHHHHHHCCEEEEEECCCCCCCCCCCCCC
DAEPMSLMQMPYGPQVLWPDHCIQGSVGAQFHADLTTDRADLIIRKGYNPAIDSYSAFFE
CCCCHHHHCCCCCCEEECCHHHCCCCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHC
NDHTTPTGLEGYLRTRGITDLTLVGLATDFCVNYSAVDAAKLGFTVTIRTDLCRAIDFDG
CCCCCCCCCCHHHHHCCCCCEEHHHHHHHHHCCCCCCCHHHCCEEEEEHHHHHHHCCCCC
SLTNALTAMHAAGATIV
CHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TQALLVIDVQNDFCPGGALAVTDGDTIVPGINALMAEFNAVILTQDWHPAGHSSFASSH
CCEEEEEECCCCCCCCCEEEEECCCEECCCHHHHHHHCCEEEEEECCCCCCCCCCCCCC
DAEPMSLMQMPYGPQVLWPDHCIQGSVGAQFHADLTTDRADLIIRKGYNPAIDSYSAFFE
CCCCHHHHCCCCCCEEECCHHHCCCCCCCEEEECCCCCCEEEEEECCCCCCHHHHHHHHC
NDHTTPTGLEGYLRTRGITDLTLVGLATDFCVNYSAVDAAKLGFTVTIRTDLCRAIDFDG
CCCCCCCCCCHHHHHCCCCCEEHHHHHHHHHCCCCCCCHHHCCEEEEEHHHHHHHCCCCC
SLTNALTAMHAAGATIV
CHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]