Click here to switch to the map view.

The map label for this gene is gltP [H]

Identifier: 88658620

GI number: 88658620

Start: 589788

End: 590924

Strand: Direct

Name: gltP [H]

Synonym: ECH_0581

Alternate gene names: 88658620

Gene position: 589788-590924 (Clockwise)

Preceding gene: 88657850

Following gene: 88658369

Centisome position: 50.14

GC content: 30.52

Gene sequence:

>1137_bases
TTGGGAGATATTTTTATTAACTTACTCAAACTAATTAGCTTACCAGTAGTATTTTTTTCTATTACATCGACAATTTCTGG
ATTGGCAAATTTGACAGAAATTAAAACTTTAATAAGAAAAACCATATTTTATACTATATCTACTACAGTGATTGCAGCTA
CTGTAGGTCTTATCACATACCTATTAATTGATCCATCAAAAAAAGAACTTATATATAACATATTGAGTACCAATAAGCAT
ATTAGCAATACTCCAGATTATTTATCGTATTTAATGTCTATATTACCTTATAACTTCATTAAGGTATTTCTAGATAATAA
TGTAATTGGGTGTGTCATACTTGCATTCCTTATAGGAGGAGCACTGCTATTATTGCCTGATAAAAATAAACGCGAATTGC
TTCATAAAGTTTTTGATGCCCTCTTCGATACATTTTTAGAAATAGCAAAGCTTATATTAAAGCTAATGCCCATAGCATTA
TGGTCATTTATTACTGTACTGTTATATAACATGAAGGAAGGATATAACATATCAAATGTTTTAAAATATCTGTTATGTAT
CATGATAGCAAATTTCATACAAGCATGTATAATACTGCCTCTGTTACTAAAATTAAAGAAGATTCCTGTTATAAAAACAT
TTCGCGGTGTTTTGCCAGCTTTAACCATAGCTTTCTTTTCAAAATCTTCTACAGCTACTCTACCAACAACTCTTCGTTGC
ACACAAGATTACTTAAATATTCCAAAAAAGATATCATCTTTTATACTGCCAGTTTGTACAACTATTAATATGAATGCATG
TGCTGCTTTCATATTAATCACAGTATTTTTTGTATCAGAAGTTAACGGATACACATTTTCTATTGGTGAGATGTTTTTGT
GGGTGTTCCTAGCTACTGGAGCAGCTATTGGTAATGCAGGGGTTCCAATGGGGTGCTACTTTATGGCTATGAGTTATCTC
ATGTCAATGAAAGTGCCTTTGAGTATCATGGGAGTTATATTGCCTGTATATACAATAATAGATATGTTTGAAACTGCAAT
TAATGTATGGTCAGATGTATGCATTACTCAGATAGTACATAAAGAATATGATGCGTTGATAAAAAAAGGTAAGAAAATTG
GAATAAATGATCAATGA

Upstream 100 bases:

>100_bases
TGTGATGTATAAATTTGTTTTAAAAATCTTTGTTCCCATAATTCTTGCAGTAATTTCATATTATATAAATCAATCATGGA
TAACTGAATTGGCTAAAGCG

Downstream 100 bases:

>100_bases
ACTATTTTTAGTATGTATCTTAATATATAAGCTATTTTAGTAAATATGTTAATTTTAATTTCAGAAGTATGGTAAGTATT
TGACTTAATTTGATAAGTAG

Product: sodium:dicarboxylate symporter family protein

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MGDIFINLLKLISLPVVFFSITSTISGLANLTEIKTLIRKTIFYTISTTVIAATVGLITYLLIDPSKKELIYNILSTNKH
ISNTPDYLSYLMSILPYNFIKVFLDNNVIGCVILAFLIGGALLLLPDKNKRELLHKVFDALFDTFLEIAKLILKLMPIAL
WSFITVLLYNMKEGYNISNVLKYLLCIMIANFIQACIILPLLLKLKKIPVIKTFRGVLPALTIAFFSKSSTATLPTTLRC
TQDYLNIPKKISSFILPVCTTINMNACAAFILITVFFVSEVNGYTFSIGEMFLWVFLATGAAIGNAGVPMGCYFMAMSYL
MSMKVPLSIMGVILPVYTIIDMFETAINVWSDVCITQIVHKEYDALIKKGKKIGINDQ

Sequences:

>Translated_378_residues
MGDIFINLLKLISLPVVFFSITSTISGLANLTEIKTLIRKTIFYTISTTVIAATVGLITYLLIDPSKKELIYNILSTNKH
ISNTPDYLSYLMSILPYNFIKVFLDNNVIGCVILAFLIGGALLLLPDKNKRELLHKVFDALFDTFLEIAKLILKLMPIAL
WSFITVLLYNMKEGYNISNVLKYLLCIMIANFIQACIILPLLLKLKKIPVIKTFRGVLPALTIAFFSKSSTATLPTTLRC
TQDYLNIPKKISSFILPVCTTINMNACAAFILITVFFVSEVNGYTFSIGEMFLWVFLATGAAIGNAGVPMGCYFMAMSYL
MSMKVPLSIMGVILPVYTIIDMFETAINVWSDVCITQIVHKEYDALIKKGKKIGINDQ
>Mature_377_residues
GDIFINLLKLISLPVVFFSITSTISGLANLTEIKTLIRKTIFYTISTTVIAATVGLITYLLIDPSKKELIYNILSTNKHI
SNTPDYLSYLMSILPYNFIKVFLDNNVIGCVILAFLIGGALLLLPDKNKRELLHKVFDALFDTFLEIAKLILKLMPIALW
SFITVLLYNMKEGYNISNVLKYLLCIMIANFIQACIILPLLLKLKKIPVIKTFRGVLPALTIAFFSKSSTATLPTTLRCT
QDYLNIPKKISSFILPVCTTINMNACAAFILITVFFVSEVNGYTFSIGEMFLWVFLATGAAIGNAGVPMGCYFMAMSYLM
SMKVPLSIMGVILPVYTIIDMFETAINVWSDVCITQIVHKEYDALIKKGKKIGINDQ

Specific function: This carrier protein is part of the Na(+)-independent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI66773030, Length=271, Percent_Identity=29.1512915129151, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI21314632, Length=272, Percent_Identity=28.6764705882353, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI4827012, Length=267, Percent_Identity=28.0898876404494, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI169790839, Length=257, Percent_Identity=28.4046692607004, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI40254478, Length=409, Percent_Identity=23.960880195599, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI223468566, Length=249, Percent_Identity=27.3092369477912, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI223468564, Length=261, Percent_Identity=26.4367816091954, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI194239697, Length=300, Percent_Identity=28, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI5032093, Length=334, Percent_Identity=25.748502994012, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI301601644, Length=142, Percent_Identity=31.6901408450704, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI262359914, Length=188, Percent_Identity=28.1914893617021, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1790514, Length=378, Percent_Identity=24.0740740740741, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788024, Length=368, Percent_Identity=27.1739130434783, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789947, Length=377, Percent_Identity=19.893899204244, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71996953, Length=429, Percent_Identity=24.9417249417249, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17537407, Length=393, Percent_Identity=25.6997455470738, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71983106, Length=444, Percent_Identity=24.3243243243243, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71983099, Length=444, Percent_Identity=24.3243243243243, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=402, Percent_Identity=24.1293532338308, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI193206654, Length=414, Percent_Identity=23.6714975845411, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17541374, Length=430, Percent_Identity=23.0232558139535, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24583025, Length=397, Percent_Identity=24.1813602015113, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17137668, Length=397, Percent_Identity=24.1813602015113, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24583023, Length=397, Percent_Identity=24.1813602015113, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281360481, Length=349, Percent_Identity=22.3495702005731, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281360483, Length=350, Percent_Identity=22.2857142857143, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17137666, Length=350, Percent_Identity=22.2857142857143, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 42198; Mature: 42066

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDIFINLLKLISLPVVFFSITSTISGLANLTEIKTLIRKTIFYTISTTVIAATVGLITY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLIDPSKKELIYNILSTNKHISNTPDYLSYLMSILPYNFIKVFLDNNVIGCVILAFLIGG
HHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
ALLLLPDKNKRELLHKVFDALFDTFLEIAKLILKLMPIALWSFITVLLYNMKEGYNISNV
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LKYLLCIMIANFIQACIILPLLLKLKKIPVIKTFRGVLPALTIAFFSKSSTATLPTTLRC
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
TQDYLNIPKKISSFILPVCTTINMNACAAFILITVFFVSEVNGYTFSIGEMFLWVFLATG
HHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHH
AAIGNAGVPMGCYFMAMSYLMSMKVPLSIMGVILPVYTIIDMFETAINVWSDVCITQIVH
HHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEYDALIKKGKKIGINDQ
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GDIFINLLKLISLPVVFFSITSTISGLANLTEIKTLIRKTIFYTISTTVIAATVGLITY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLIDPSKKELIYNILSTNKHISNTPDYLSYLMSILPYNFIKVFLDNNVIGCVILAFLIGG
HHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
ALLLLPDKNKRELLHKVFDALFDTFLEIAKLILKLMPIALWSFITVLLYNMKEGYNISNV
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LKYLLCIMIANFIQACIILPLLLKLKKIPVIKTFRGVLPALTIAFFSKSSTATLPTTLRC
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
TQDYLNIPKKISSFILPVCTTINMNACAAFILITVFFVSEVNGYTFSIGEMFLWVFLATG
HHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHH
AAIGNAGVPMGCYFMAMSYLMSMKVPLSIMGVILPVYTIIDMFETAINVWSDVCITQIVH
HHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEYDALIKKGKKIGINDQ
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1971622; 1551855; 8265357; 9278503 [H]