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The map label for this gene is fabI [H]

Identifier: 88658552

GI number: 88658552

Start: 824171

End: 824971

Strand: Reverse

Name: fabI [H]

Synonym: ECH_0811

Alternate gene names: 88658552

Gene position: 824971-824171 (Counterclockwise)

Preceding gene: 88658061

Following gene: 88658534

Centisome position: 70.14

GC content: 33.96

Gene sequence:

>801_bases
ATGAAAGGAAAAAAAGGATTAGTCATTGGAATTGCTAATGATAAATCTTTAGCATGGGGGATTGCTAAAACTTTAGCTAT
GCATGGGGCAGATATAGCAGTGACTTATTTATCCGACGTTTTTAAGAAAAGGATAGATCCTTTAGCGGAATCTATAGGTG
TAAAGTTGGTCTTGCAATGTGATGTATCTAAAGAAGAGACTGTAGATAATATGTTTGCACAGCTTAGTGATGAATGGGAA
AGTATGGATTTTTTAGTACATGCTGTTGCTTTTTCTGATAAAAATGAGCTGAAAGGTAGGTATATTGATACGTCATTGAA
TAATTTTTTGACATCTATGCATATTTCATGTTACTCGCTTACTTACCTTGCTAAAAAAGTAGAGAAGATGATGTGTAATG
GAGGTAGTATATTAACATTATCTTATTATGGTGCTCAAAAAGTGATGCCTCACTATAATGTGATGGGAGTATGTAAAGCA
GCTTTAGAAGCAAGTGTAAAGTATCTGGCTGTTGATTTAGGTTCTCAGAATATTAGGGTTAATGCTATTTCTGCAGGTCC
AGTAAGAACTTTAGCATCTTCTGGGATAGGAGATTTCCATTACATATTGACATGGAATAAATATAATGCACCTTTGAGGC
GTAATACTACAATAGACGATGTGGGTGGAGCAGCACTTTATTTATTAAGTGATTTAGGTAAAGGTACTACAGGAGAAATT
TTATATGTTGATGGTGGATATCATGCTATAGGTATGAAAGCAATAGATGCTCCTGATATTAATTATATTGAAGCCAAATA
A

Upstream 100 bases:

>100_bases
ATCTTGAATTTTTTTGTTTTATTTATAAGATATTAATTAATGTAGTATTGTAAGAGCATGTCTAAAAAATAAGGAGATAT
CAGTGCTTGCTAGTGATATG

Downstream 100 bases:

>100_bases
AATGAATATTATTTATATAAAGTTGTGATATTTTAATTATAGTATTGAGTTTTTTTATTTACTTTACTCGTGATGTTTCT
ATTTCTTTCTTAGTTATACA

Product: enoyl-(acyl-carrier-protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKGKKGLVIGIANDKSLAWGIAKTLAMHGADIAVTYLSDVFKKRIDPLAESIGVKLVLQCDVSKEETVDNMFAQLSDEWE
SMDFLVHAVAFSDKNELKGRYIDTSLNNFLTSMHISCYSLTYLAKKVEKMMCNGGSILTLSYYGAQKVMPHYNVMGVCKA
ALEASVKYLAVDLGSQNIRVNAISAGPVRTLASSGIGDFHYILTWNKYNAPLRRNTTIDDVGGAALYLLSDLGKGTTGEI
LYVDGGYHAIGMKAIDAPDINYIEAK

Sequences:

>Translated_266_residues
MKGKKGLVIGIANDKSLAWGIAKTLAMHGADIAVTYLSDVFKKRIDPLAESIGVKLVLQCDVSKEETVDNMFAQLSDEWE
SMDFLVHAVAFSDKNELKGRYIDTSLNNFLTSMHISCYSLTYLAKKVEKMMCNGGSILTLSYYGAQKVMPHYNVMGVCKA
ALEASVKYLAVDLGSQNIRVNAISAGPVRTLASSGIGDFHYILTWNKYNAPLRRNTTIDDVGGAALYLLSDLGKGTTGEI
LYVDGGYHAIGMKAIDAPDINYIEAK
>Mature_266_residues
MKGKKGLVIGIANDKSLAWGIAKTLAMHGADIAVTYLSDVFKKRIDPLAESIGVKLVLQCDVSKEETVDNMFAQLSDEWE
SMDFLVHAVAFSDKNELKGRYIDTSLNNFLTSMHISCYSLTYLAKKVEKMMCNGGSILTLSYYGAQKVMPHYNVMGVCKA
ALEASVKYLAVDLGSQNIRVNAISAGPVRTLASSGIGDFHYILTWNKYNAPLRRNTTIDDVGGAALYLLSDLGKGTTGEI
LYVDGGYHAIGMKAIDAPDINYIEAK

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=200, Percent_Identity=27.5, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787545, Length=257, Percent_Identity=43.1906614785992, Blast_Score=236, Evalue=9e-64,
Organism=Escherichia coli, GI1789378, Length=255, Percent_Identity=24.7058823529412, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1787905, Length=229, Percent_Identity=26.6375545851528, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082100, Length=255, Percent_Identity=26.6666666666667, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28983; Mature: 28983

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKKGLVIGIANDKSLAWGIAKTLAMHGADIAVTYLSDVFKKRIDPLAESIGVKLVLQC
CCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEE
DVSKEETVDNMFAQLSDEWESMDFLVHAVAFSDKNELKGRYIDTSLNNFLTSMHISCYSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEECHHHHHHHHHHHHHHHHH
TYLAKKVEKMMCNGGSILTLSYYGAQKVMPHYNVMGVCKAALEASVKYLAVDLGSQNIRV
HHHHHHHHHHHCCCCCEEEEEECCCHHCCCCCCHHHHHHHHHHCCEEEEEEEECCCEEEE
NAISAGPVRTLASSGIGDFHYILTWNKYNAPLRRNTTIDDVGGAALYLLSDLGKGTTGEI
EEECCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEE
LYVDGGYHAIGMKAIDAPDINYIEAK
EEEECCEEEEEEEEECCCCCCEEECC
>Mature Secondary Structure
MKGKKGLVIGIANDKSLAWGIAKTLAMHGADIAVTYLSDVFKKRIDPLAESIGVKLVLQC
CCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEE
DVSKEETVDNMFAQLSDEWESMDFLVHAVAFSDKNELKGRYIDTSLNNFLTSMHISCYSL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEECHHHHHHHHHHHHHHHHH
TYLAKKVEKMMCNGGSILTLSYYGAQKVMPHYNVMGVCKAALEASVKYLAVDLGSQNIRV
HHHHHHHHHHHCCCCCEEEEEECCCHHCCCCCCHHHHHHHHHHCCEEEEEEEECCCEEEE
NAISAGPVRTLASSGIGDFHYILTWNKYNAPLRRNTTIDDVGGAALYLLSDLGKGTTGEI
EEECCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEE
LYVDGGYHAIGMKAIDAPDINYIEAK
EEEECCEEEEEEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]