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The map label for this gene is gatB

Identifier: 88658061

GI number: 88658061

Start: 825629

End: 827074

Strand: Reverse

Name: gatB

Synonym: ECH_0813

Alternate gene names: 88658061

Gene position: 827074-825629 (Counterclockwise)

Preceding gene: 88657593

Following gene: 88658552

Centisome position: 70.31

GC content: 33.68

Gene sequence:

>1446_bases
ATGACGATTATTAAAGGAAATAGGTGTGATTGGGAGGTAGTAATAGGGTTAGAAGTCCATGCACAAGTTATTTCCAATTC
TAAATTATTTTCTGGTGCTTCAACAAAGACTTATGATGCTTTACCTAATACGCAAGTAGCGCTTTTTGATGTAGCAATGC
CAGGTATGTTGCCTGTACTTAATGAGTATTGTGTTTATCAAGCAATAAAAACAGGAATTGCTCTATCATGTAAGATTAAC
AAGTATTCAGCTTTTGATAGAAAGAATTATTTTTATCCTGACTTACCTTCTGGTTATCAAATAACACAGTTCTATTATCC
TATAGCAACAGAAGGTAAAATTGTCTTAGAAGATCATGATATGAAGGAAATTCGCATTGCTAGAATTCATCTTGAGCAAG
ATGCAGGAAAAAGTATTCATGAATTTGACAAAACTTATATAGATTTCAATAGGGCAGGTGTTGCATTAATGGAAATAGTG
TCAGAGCCTGATTTCAGATCTATTGAAGAGGTTGCAGAATATCTGAAGAAGTTACGTATGATATTGCGTTTTATAGAAAC
ATGTGATGGAGATATGGAAAAAGGTTCTTTACGTTGTGATGCAAATGTTTCAGTCAAACCTGTTGGAAGTAGTGAGCTTG
GAATAAGGTCTGAAATAAAAAATCTTAATTCCATTCGTTATGTTATGCAAGCTATCGAGTATGAAGCAAATAGACAGGTT
AATGCTTTGGAAAATGGAGAAATAGTTACTCAAAATACGTTGTTGTTTGATGTTACTTCAGGGCAAACAAGAGTAATTAG
GACTAAAGAAGATGCTCATGATTATCGATATTTTCCAGATCCTGACTTATTTCCATTAAAGATTGATGATCAATATATAG
ATCATGTCAGGTCGTCTTTACCAGAACTACCTATGCAGAAAAGAGAGCGTTATACTAATGATTTTAGTCTAAGTAAGTAT
GATGCAGATATTTTATCTTCTGATAAGGATGTAGCTATATATTTTGAAAAGGTTGCAGAGAAGCATGATGGCAAACTTGC
AGCATCTTGGATAACAGGTGAGCTTTTTGGTAGACTAAATAGGCTTGGAATTACTATTGGTGAATCTTCTGTGACAGCTG
AAGACTTGATACAGCTGCTTGATTTGATAGTCAATAATACTATTTCTGGAAAAATAGCAAAGCAAGTGTTTGATATGATG
TTTGAGTCAGGTAAATCACCTGCATTGATAGTCAGTGAACATGGGTTAAAGCAGGTAAGCGATGAGAATGCTTTATCTGT
AATTGTGGAAAGGGTTTTAAAAAATAATGCAAGTAAAGTAGTAGAATATAAACAAGGTAAAGAAAAATTATTTGGGTATT
TTGTTGGGCAGGTAATGAAAGAAACGCAAGGCAAAGCAAACCCTGATATGGTAAATTCTATTATCAAGCAGCAGTTAGAA
AATTAA

Upstream 100 bases:

>100_bases
TGAAAACTATGTAATTAATAGAGAGATTAATTGTTCAAAAGAAGTGTTGTCACTATTAAAGCGTTGATTTAATATGTTTT
ATTTAAAGTGATATATTTTT

Downstream 100 bases:

>100_bases
TAGTTATGAGTTATTGATGAATATAAAATCATATTAACTATATTAAATAAAGTTTTATTGTTTATCTATTTCTCAATGCT
AATTGTATATTGTTGAATTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MTIIKGNRCDWEVVIGLEVHAQVISNSKLFSGASTKTYDALPNTQVALFDVAMPGMLPVLNEYCVYQAIKTGIALSCKIN
KYSAFDRKNYFYPDLPSGYQITQFYYPIATEGKIVLEDHDMKEIRIARIHLEQDAGKSIHEFDKTYIDFNRAGVALMEIV
SEPDFRSIEEVAEYLKKLRMILRFIETCDGDMEKGSLRCDANVSVKPVGSSELGIRSEIKNLNSIRYVMQAIEYEANRQV
NALENGEIVTQNTLLFDVTSGQTRVIRTKEDAHDYRYFPDPDLFPLKIDDQYIDHVRSSLPELPMQKRERYTNDFSLSKY
DADILSSDKDVAIYFEKVAEKHDGKLAASWITGELFGRLNRLGITIGESSVTAEDLIQLLDLIVNNTISGKIAKQVFDMM
FESGKSPALIVSEHGLKQVSDENALSVIVERVLKNNASKVVEYKQGKEKLFGYFVGQVMKETQGKANPDMVNSIIKQQLE
N

Sequences:

>Translated_481_residues
MTIIKGNRCDWEVVIGLEVHAQVISNSKLFSGASTKTYDALPNTQVALFDVAMPGMLPVLNEYCVYQAIKTGIALSCKIN
KYSAFDRKNYFYPDLPSGYQITQFYYPIATEGKIVLEDHDMKEIRIARIHLEQDAGKSIHEFDKTYIDFNRAGVALMEIV
SEPDFRSIEEVAEYLKKLRMILRFIETCDGDMEKGSLRCDANVSVKPVGSSELGIRSEIKNLNSIRYVMQAIEYEANRQV
NALENGEIVTQNTLLFDVTSGQTRVIRTKEDAHDYRYFPDPDLFPLKIDDQYIDHVRSSLPELPMQKRERYTNDFSLSKY
DADILSSDKDVAIYFEKVAEKHDGKLAASWITGELFGRLNRLGITIGESSVTAEDLIQLLDLIVNNTISGKIAKQVFDMM
FESGKSPALIVSEHGLKQVSDENALSVIVERVLKNNASKVVEYKQGKEKLFGYFVGQVMKETQGKANPDMVNSIIKQQLE
N
>Mature_480_residues
TIIKGNRCDWEVVIGLEVHAQVISNSKLFSGASTKTYDALPNTQVALFDVAMPGMLPVLNEYCVYQAIKTGIALSCKINK
YSAFDRKNYFYPDLPSGYQITQFYYPIATEGKIVLEDHDMKEIRIARIHLEQDAGKSIHEFDKTYIDFNRAGVALMEIVS
EPDFRSIEEVAEYLKKLRMILRFIETCDGDMEKGSLRCDANVSVKPVGSSELGIRSEIKNLNSIRYVMQAIEYEANRQVN
ALENGEIVTQNTLLFDVTSGQTRVIRTKEDAHDYRYFPDPDLFPLKIDDQYIDHVRSSLPELPMQKRERYTNDFSLSKYD
ADILSSDKDVAIYFEKVAEKHDGKLAASWITGELFGRLNRLGITIGESSVTAEDLIQLLDLIVNNTISGKIAKQVFDMMF
ESGKSPALIVSEHGLKQVSDENALSVIVERVLKNNASKVVEYKQGKEKLFGYFVGQVMKETQGKANPDMVNSIIKQQLEN

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=40.6060606060606, Blast_Score=356, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI115534094, Length=300, Percent_Identity=42.6666666666667, Blast_Score=226, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6319391, Length=510, Percent_Identity=32.3529411764706, Blast_Score=233, Evalue=6e-62,
Organism=Drosophila melanogaster, GI45550808, Length=487, Percent_Identity=36.3449691991786, Blast_Score=320, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_EHRCR (Q2GG24)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507609.1
- ProteinModelPortal:   Q2GG24
- SMR:   Q2GG24
- STRING:   Q2GG24
- GeneID:   3927248
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0813
- TIGR:   ECH_0813
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- PhylomeDB:   Q2GG24
- ProtClustDB:   PRK05477
- BioCyc:   ECHA205920:ECH_0813-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: NA

Molecular weight: Translated: 54437; Mature: 54306

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIKGNRCDWEVVIGLEVHAQVISNSKLFSGASTKTYDALPNTQVALFDVAMPGMLPVL
CEEECCCCCCEEEEEEEEEHHHHHCCCCEECCCCCCCCCCCCCCEEEEEEECCCCHHHHH
NEYCVYQAIKTGIALSCKINKYSAFDRKNYFYPDLPSGYQITQFYYPIATEGKIVLEDHD
HHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEECCC
MKEIRIARIHLEQDAGKSIHEFDKTYIDFNRAGVALMEIVSEPDFRSIEEVAEYLKKLRM
CCCEEEEEEEECCCCCCHHHHHHHHHEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ILRFIETCDGDMEKGSLRCDANVSVKPVGSSELGIRSEIKNLNSIRYVMQAIEYEANRQV
HHHHHHHCCCCCCCCCEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
NALENGEIVTQNTLLFDVTSGQTRVIRTKEDAHDYRYFPDPDLFPLKIDDQYIDHVRSSL
CCCCCCCEEEECEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEECHHHHHHHHHHC
PELPMQKRERYTNDFSLSKYDADILSSDKDVAIYFEKVAEKHDGKLAASWITGELFGRLN
CCCCHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
RLGITIGESSVTAEDLIQLLDLIVNNTISGKIAKQVFDMMFESGKSPALIVSEHGLKQVS
HHEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHCC
DENALSVIVERVLKNNASKVVEYKQGKEKLFGYFVGQVMKETQGKANPDMVNSIIKQQLE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
N
C
>Mature Secondary Structure 
TIIKGNRCDWEVVIGLEVHAQVISNSKLFSGASTKTYDALPNTQVALFDVAMPGMLPVL
EEECCCCCCEEEEEEEEEHHHHHCCCCEECCCCCCCCCCCCCCEEEEEEECCCCHHHHH
NEYCVYQAIKTGIALSCKINKYSAFDRKNYFYPDLPSGYQITQFYYPIATEGKIVLEDHD
HHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEECCC
MKEIRIARIHLEQDAGKSIHEFDKTYIDFNRAGVALMEIVSEPDFRSIEEVAEYLKKLRM
CCCEEEEEEEECCCCCCHHHHHHHHHEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ILRFIETCDGDMEKGSLRCDANVSVKPVGSSELGIRSEIKNLNSIRYVMQAIEYEANRQV
HHHHHHHCCCCCCCCCEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
NALENGEIVTQNTLLFDVTSGQTRVIRTKEDAHDYRYFPDPDLFPLKIDDQYIDHVRSSL
CCCCCCCEEEECEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEECHHHHHHHHHHC
PELPMQKRERYTNDFSLSKYDADILSSDKDVAIYFEKVAEKHDGKLAASWITGELFGRLN
CCCCHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
RLGITIGESSVTAEDLIQLLDLIVNNTISGKIAKQVFDMMFESGKSPALIVSEHGLKQVS
HHEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHCC
DENALSVIVERVLKNNASKVVEYKQGKEKLFGYFVGQVMKETQGKANPDMVNSIIKQQLE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA