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The map label for this gene is recG [H]

Identifier: 88658514

GI number: 88658514

Start: 55011

End: 57050

Strand: Direct

Name: recG [H]

Synonym: ECH_0062

Alternate gene names: 88658514

Gene position: 55011-57050 (Clockwise)

Preceding gene: 88658612

Following gene: 88658323

Centisome position: 4.68

GC content: 31.67

Gene sequence:

>2040_bases
ATGCACAACTTAAGCATATTTTCTAGCATGTATATGTTGCCAGGAGTTAATAATGTAGTAGGGAGTTTATTAAAAAAGTT
ATGTGGTGGTGATAAAATTATAGATTTGTTATTTCATATACCACAAAGTTATGTGGATAGAAGAACTGCGTTATCTGAAG
ATTCTGTAGGTAAAATTGTAACTTTTATTGGTACTGTGAAGTGTCATGGTTTTATTGGTAGGAAAAGAAAATCCCAGTAT
AAAATTGTGCTTGATACATGTATTGGTGAAGTATCATTAATTTTTTTTAATTACTCGTTGAAATATCTGAGAAGTGTTTT
AAAGGTAGGTTCAACATGTGTAGTAAGTGGTACTCTGATAAGATTTTTGGGATGTTTGCAAATTACACATCCGGATTATA
TTGTAACAGATATTAAAAAGTTCCAGGATATTAGTATCATAGAACCTATTTATCCACTAATTAGAGGATTAACTTCTAAG
AGAATTTCAAAGTTAGTAAAGTTAAGTGTAAGGTTGTTACCTGATTTTCCTGAATGGATAGATGAAAAATTGTTAAGAGA
TAATAGATGGAATAGTTGGAAGGAAAGTTTAATAAAAATACATCACCCAGATACACTTGAAGCTGTGCATTTACATAGGG
CAAGGTTAGCGTATGATGAATTATTAAGTCATCAAATCTCTGTAAAAATGGTCAGGAAATTTGATTACCAACAAGGTGTC
TCTATTGTAAGTAAGCAGCTATACTATAATGATATTTTAAATAAACTGCCTTTTAAGTTAACAGAAGGACAGAAAGAAGT
AATATCTCAGATTACAAAGAGTCAAGCATCAGAAAATAGGATGGTAAAACTATTAATAGGAGATGTAGGTAGTGGGAAAA
CAGTGGTAGCTTTGTTTGCTATTTTAAATGTTATAGAGAATGGAGGTCAAGTAGCTTTTATGGCTCCTACTGAGATATTA
GCAGAACAACATTATCGTTGGATACGGGCGATACTATCTGATATTTCAGTTGATGTTGAGTTGTTAACAAGTAAGGTAAG
GAAGAAACAGAATATTAAAAAGAAATTGCAACTTGGTGAATGTAATGTAGTTATTGGAACTCATGCTTTATTTCAGGATG
GTGTTGATTTTAATAATCTTAATTTGATTATCATTGATGAACAACAAAGATTTGGAGTATTACAGAGGATGAGGCTAATT
AATAAAAGTAATATGGCTGATGTGCTTTTTATGACAGCTACGCCTATTCCTAGAACATTAGAGCAAGTAGTATATGGGGA
TATAGATTGTTTAAGACTTAAGGATAAGCCTCACAATCGATTGCCTATACAAACTAGTATTGTGAATATTGAGCGCTTGT
TTGAAGTTATAGCAAAATTACAATTGGCTTTGCAAGAAGGTAATAAGGCATATTGGATTTGTCCTTATATTGAAGATTCG
GAATTATTAGATATAGCAGCAGCAGAAAAACGTTTTTTTACTCTACAAGAAGTGTTTGGTAAGGATGTAGGTTTGATACA
CAGTCGTTTGCCTAAGATTGAAAAAGATGAAGTCATGATGTCTTTTTATAATGGAAATATTAAGTTACTAGTTGCAACTA
CTGTAATAGAAGTAGGAGTTGATATACCTGATGCTACTATAATTATTATAGAAAATGCTGAACAATTTGGTCTATCACAG
CTTCATCAATTACGTGGTAGAGTTGGCCGTAGTGATAAATCTTCTTTTTGTATTTTACTTCATGGGAATATGTTAAGTAA
GATTGCATATAAAAAGCTATGTATATTGCGTAAATTTCAAGATGGCTTTTACATAGCTGAGCAGGATTTGTTGTTGCGTG
GTAGTGGAGATGTTTTAGGGATAAAACAATCAGGATTATCAAATTTTAAGTTTGCAGATATTTATAAGGATCAAGGCCTT
ATTTCTATTGCTGTTGAACAGGCAGGAGCAATTCTGGATGCGAATAAGACAGAATTAGGTGATCATTTTAATCAATTGTT
ACACATGTTTGGTTATGATGTTTCTACTATAAATTATTAG

Upstream 100 bases:

>100_bases
ACTAGGTAAACATTCAATTTCTATCATATATTTTTGTATATTCAAAGTTTAATATTTATAATAACTTTGACGTATTAGTT
TAATGTAGGATTATAGTTTA

Downstream 100 bases:

>100_bases
TTTTTATGTGAGTAAGGGATTTTGATAAATTTCAAGTGGTAGTGATTTTTAGAGAAGAGTATATCAGTTTAAGTTTTGAA
TAGAAAATTCTGGCTATAAT

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 679; Mature: 679

Protein sequence:

>679_residues
MHNLSIFSSMYMLPGVNNVVGSLLKKLCGGDKIIDLLFHIPQSYVDRRTALSEDSVGKIVTFIGTVKCHGFIGRKRKSQY
KIVLDTCIGEVSLIFFNYSLKYLRSVLKVGSTCVVSGTLIRFLGCLQITHPDYIVTDIKKFQDISIIEPIYPLIRGLTSK
RISKLVKLSVRLLPDFPEWIDEKLLRDNRWNSWKESLIKIHHPDTLEAVHLHRARLAYDELLSHQISVKMVRKFDYQQGV
SIVSKQLYYNDILNKLPFKLTEGQKEVISQITKSQASENRMVKLLIGDVGSGKTVVALFAILNVIENGGQVAFMAPTEIL
AEQHYRWIRAILSDISVDVELLTSKVRKKQNIKKKLQLGECNVVIGTHALFQDGVDFNNLNLIIIDEQQRFGVLQRMRLI
NKSNMADVLFMTATPIPRTLEQVVYGDIDCLRLKDKPHNRLPIQTSIVNIERLFEVIAKLQLALQEGNKAYWICPYIEDS
ELLDIAAAEKRFFTLQEVFGKDVGLIHSRLPKIEKDEVMMSFYNGNIKLLVATTVIEVGVDIPDATIIIIENAEQFGLSQ
LHQLRGRVGRSDKSSFCILLHGNMLSKIAYKKLCILRKFQDGFYIAEQDLLLRGSGDVLGIKQSGLSNFKFADIYKDQGL
ISIAVEQAGAILDANKTELGDHFNQLLHMFGYDVSTINY

Sequences:

>Translated_679_residues
MHNLSIFSSMYMLPGVNNVVGSLLKKLCGGDKIIDLLFHIPQSYVDRRTALSEDSVGKIVTFIGTVKCHGFIGRKRKSQY
KIVLDTCIGEVSLIFFNYSLKYLRSVLKVGSTCVVSGTLIRFLGCLQITHPDYIVTDIKKFQDISIIEPIYPLIRGLTSK
RISKLVKLSVRLLPDFPEWIDEKLLRDNRWNSWKESLIKIHHPDTLEAVHLHRARLAYDELLSHQISVKMVRKFDYQQGV
SIVSKQLYYNDILNKLPFKLTEGQKEVISQITKSQASENRMVKLLIGDVGSGKTVVALFAILNVIENGGQVAFMAPTEIL
AEQHYRWIRAILSDISVDVELLTSKVRKKQNIKKKLQLGECNVVIGTHALFQDGVDFNNLNLIIIDEQQRFGVLQRMRLI
NKSNMADVLFMTATPIPRTLEQVVYGDIDCLRLKDKPHNRLPIQTSIVNIERLFEVIAKLQLALQEGNKAYWICPYIEDS
ELLDIAAAEKRFFTLQEVFGKDVGLIHSRLPKIEKDEVMMSFYNGNIKLLVATTVIEVGVDIPDATIIIIENAEQFGLSQ
LHQLRGRVGRSDKSSFCILLHGNMLSKIAYKKLCILRKFQDGFYIAEQDLLLRGSGDVLGIKQSGLSNFKFADIYKDQGL
ISIAVEQAGAILDANKTELGDHFNQLLHMFGYDVSTINY
>Mature_679_residues
MHNLSIFSSMYMLPGVNNVVGSLLKKLCGGDKIIDLLFHIPQSYVDRRTALSEDSVGKIVTFIGTVKCHGFIGRKRKSQY
KIVLDTCIGEVSLIFFNYSLKYLRSVLKVGSTCVVSGTLIRFLGCLQITHPDYIVTDIKKFQDISIIEPIYPLIRGLTSK
RISKLVKLSVRLLPDFPEWIDEKLLRDNRWNSWKESLIKIHHPDTLEAVHLHRARLAYDELLSHQISVKMVRKFDYQQGV
SIVSKQLYYNDILNKLPFKLTEGQKEVISQITKSQASENRMVKLLIGDVGSGKTVVALFAILNVIENGGQVAFMAPTEIL
AEQHYRWIRAILSDISVDVELLTSKVRKKQNIKKKLQLGECNVVIGTHALFQDGVDFNNLNLIIIDEQQRFGVLQRMRLI
NKSNMADVLFMTATPIPRTLEQVVYGDIDCLRLKDKPHNRLPIQTSIVNIERLFEVIAKLQLALQEGNKAYWICPYIEDS
ELLDIAAAEKRFFTLQEVFGKDVGLIHSRLPKIEKDEVMMSFYNGNIKLLVATTVIEVGVDIPDATIIIIENAEQFGLSQ
LHQLRGRVGRSDKSSFCILLHGNMLSKIAYKKLCILRKFQDGFYIAEQDLLLRGSGDVLGIKQSGLSNFKFADIYKDQGL
ISIAVEQAGAILDANKTELGDHFNQLLHMFGYDVSTINY

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=639, Percent_Identity=37.5586854460094, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1787357, Length=396, Percent_Identity=34.5959595959596, Blast_Score=226, Evalue=5e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76965; Mature: 76965

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNLSIFSSMYMLPGVNNVVGSLLKKLCGGDKIIDLLFHIPQSYVDRRTALSEDSVGKIV
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHH
TFIGTVKCHGFIGRKRKSQYKIVLDTCIGEVSLIFFNYSLKYLRSVLKVGSTCVVSGTLI
HHHHHHHHCCHHCCCCCCCCEEEHHHHHCHHEEEEEHHHHHHHHHHHHCCCCEEEHHHHH
RFLGCLQITHPDYIVTDIKKFQDISIIEPIYPLIRGLTSKRISKLVKLSVRLLPDFPEWI
HHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
DEKLLRDNRWNSWKESLIKIHHPDTLEAVHLHRARLAYDELLSHQISVKMVRKFDYQQGV
HHHHHHCCCCCHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
SIVSKQLYYNDILNKLPFKLTEGQKEVISQITKSQASENRMVKLLIGDVGSGKTVVALFA
HHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHH
ILNVIENGGQVAFMAPTEILAEQHYRWIRAILSDISVDVELLTSKVRKKQNIKKKLQLGE
HHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
CNVVIGTHALFQDGVDFNNLNLIIIDEQQRFGVLQRMRLINKSNMADVLFMTATPIPRTL
CEEEEECHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHH
EQVVYGDIDCLRLKDKPHNRLPIQTSIVNIERLFEVIAKLQLALQEGNKAYWICPYIEDS
HHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
ELLDIAAAEKRFFTLQEVFGKDVGLIHSRLPKIEKDEVMMSFYNGNIKLLVATTVIEVGV
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHEEEEECCCEEEEEEEEEHHHCC
DIPDATIIIIENAEQFGLSQLHQLRGRVGRSDKSSFCILLHGNMLSKIAYKKLCILRKFQ
CCCCCEEEEEECCHHCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHC
DGFYIAEQDLLLRGSGDVLGIKQSGLSNFKFADIYKDQGLISIAVEQAGAILDANKTELG
CCEEEECCCEEEECCCCEEEEECCCCCCCEEHHHCCCCCEEEEEEHHCCCEECCCHHHHH
DHFNQLLHMFGYDVSTINY
HHHHHHHHHHCCCCEECCC
>Mature Secondary Structure
MHNLSIFSSMYMLPGVNNVVGSLLKKLCGGDKIIDLLFHIPQSYVDRRTALSEDSVGKIV
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHH
TFIGTVKCHGFIGRKRKSQYKIVLDTCIGEVSLIFFNYSLKYLRSVLKVGSTCVVSGTLI
HHHHHHHHCCHHCCCCCCCCEEEHHHHHCHHEEEEEHHHHHHHHHHHHCCCCEEEHHHHH
RFLGCLQITHPDYIVTDIKKFQDISIIEPIYPLIRGLTSKRISKLVKLSVRLLPDFPEWI
HHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
DEKLLRDNRWNSWKESLIKIHHPDTLEAVHLHRARLAYDELLSHQISVKMVRKFDYQQGV
HHHHHHCCCCCHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
SIVSKQLYYNDILNKLPFKLTEGQKEVISQITKSQASENRMVKLLIGDVGSGKTVVALFA
HHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHH
ILNVIENGGQVAFMAPTEILAEQHYRWIRAILSDISVDVELLTSKVRKKQNIKKKLQLGE
HHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
CNVVIGTHALFQDGVDFNNLNLIIIDEQQRFGVLQRMRLINKSNMADVLFMTATPIPRTL
CEEEEECHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHH
EQVVYGDIDCLRLKDKPHNRLPIQTSIVNIERLFEVIAKLQLALQEGNKAYWICPYIEDS
HHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
ELLDIAAAEKRFFTLQEVFGKDVGLIHSRLPKIEKDEVMMSFYNGNIKLLVATTVIEVGV
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHEEEEECCCEEEEEEEEEHHHCC
DIPDATIIIIENAEQFGLSQLHQLRGRVGRSDKSSFCILLHGNMLSKIAYKKLCILRKFQ
CCCCCEEEEEECCHHCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHC
DGFYIAEQDLLLRGSGDVLGIKQSGLSNFKFADIYKDQGLISIAVEQAGAILDANKTELG
CCEEEECCCEEEECCCCEEEEECCCCCCCEEHHHCCCCCEEEEEEHHCCCEECCCHHHHH
DHFNQLLHMFGYDVSTINY
HHHHHHHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]