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The map label for this gene is shiA [H]

Identifier: 88658389

GI number: 88658389

Start: 829311

End: 830543

Strand: Reverse

Name: shiA [H]

Synonym: ECH_0816

Alternate gene names: 88658389

Gene position: 830543-829311 (Counterclockwise)

Preceding gene: 88657929

Following gene: 88657593

Centisome position: 70.61

GC content: 31.06

Gene sequence:

>1233_bases
TTGACAACTTCCAATTTAAAGCCAATGCTATCAGTACTTCTGTGTGCATTTATAGAATGTTATGATTTTTTAGTGTATGG
GAATTTTAGTAGAATATTTAGTCAGATGTTTTTTTCTCATCTAACAGAAGGTTTTGCGTTATTGTTGTCATTTATGACAT
TTGGAATAGCTTTTTTTGTTAGACCTTTTGGGTCATTGTTATTTGGTTATGTTGGTGATAAGTATGGTAGAAAAATTGCT
TTATTTAGCTCTGCAACCTTACTTATTGTTTCAGTGGGTGGTATAGCCTTTCTTCCTCTTTTTGAATCTATAGGTGTATT
ATCGCCTATTTTACTGATAGTATTTCGTATTTTACAAGGATTATCCTTTGCTGGAGAAATAGGGTCTGTAGTTTTGATGG
CAGAAAATGTAAGGGATCGTCGTAATATTGCATATGCAATGGGTAGTCATTTTCTAGTGTCAATATTTGGTGGCGCAATA
GGATGTTTTGTCTTTAAGTTGTGTTATAATTTAATTCCAGAAGCTCAATTTTATTCTTGGGGATGGAGAATACCATTTAT
TATCGGATTGATAATGTCTTTATTGTTACCTTTGTTGCGTACTTCTGTTGAAGAAAGTAGAGAGTATTTGGATTATAAAA
GTAAGCGTAAAATTTCTAGGATGCCTATTTTAGATGTCATATTCAATCATAAAAAACCTTTTATAGTAGCATGTATTTTT
GTACCTTTTTGTAATTCTTTGTTTTACACTTTCTTTGTGTTCTTGGATGTGCAAAGCAAAGTAAGTATGTCGGTGTATGC
TTTATTGATTTTAACAGTAACTTTATCGTGTTGTCTGTTTACTATGCTGTGTAGGATATATAAAACTACAACTGTTGCTC
TCTGTATTCAGGTGTTTTTTATTATAGGGATATCTCCTCTTATTTGGTTTTTTGGTAAATCTATTGTAACTTATTTTCTT
GTAGCATTTTCTTTAGGTATATGTGCAACACCTCTTATTGCTGTAATTATGCTTTTATTTCCAGCGAATGTTAGACAAAC
TGGGTATTCAGTTGCATTTAGTCTTGCAGTAGGATGTTTTAGTATGTTAACTCCTGCAGTTTTGTTATGGTTGGAAAAAT
TTAGTATACCTCCTGTACTTTGTATAGATTTTTATGCTTTACTTTCTTTATATGGTTTTTATTGTCTTAAGAAAGATAAG
AAAGTTGCTGAATATGAAAGTGTATTTGTATAA

Upstream 100 bases:

>100_bases
TGCTAAATCTTAATTAATTGTTTTATTTTTTTAATAATATTGACATGCATAACTACTGATTGTAGTTATTGTAGTAGTAG
GTTTAATTGCTATCCATATG

Downstream 100 bases:

>100_bases
TTATACTATAGGGTTATCGATTGTTTTTAAGTACAAACTTGATTGTTGATGTGTATGCTACATTTCATTGAAGTGTATAT
CATTCTTATAATCCTAGCTG

Product: major facilitator family transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTTSNLKPMLSVLLCAFIECYDFLVYGNFSRIFSQMFFSHLTEGFALLLSFMTFGIAFFVRPFGSLLFGYVGDKYGRKIA
LFSSATLLIVSVGGIAFLPLFESIGVLSPILLIVFRILQGLSFAGEIGSVVLMAENVRDRRNIAYAMGSHFLVSIFGGAI
GCFVFKLCYNLIPEAQFYSWGWRIPFIIGLIMSLLLPLLRTSVEESREYLDYKSKRKISRMPILDVIFNHKKPFIVACIF
VPFCNSLFYTFFVFLDVQSKVSMSVYALLILTVTLSCCLFTMLCRIYKTTTVALCIQVFFIIGISPLIWFFGKSIVTYFL
VAFSLGICATPLIAVIMLLFPANVRQTGYSVAFSLAVGCFSMLTPAVLLWLEKFSIPPVLCIDFYALLSLYGFYCLKKDK
KVAEYESVFV

Sequences:

>Translated_410_residues
MTTSNLKPMLSVLLCAFIECYDFLVYGNFSRIFSQMFFSHLTEGFALLLSFMTFGIAFFVRPFGSLLFGYVGDKYGRKIA
LFSSATLLIVSVGGIAFLPLFESIGVLSPILLIVFRILQGLSFAGEIGSVVLMAENVRDRRNIAYAMGSHFLVSIFGGAI
GCFVFKLCYNLIPEAQFYSWGWRIPFIIGLIMSLLLPLLRTSVEESREYLDYKSKRKISRMPILDVIFNHKKPFIVACIF
VPFCNSLFYTFFVFLDVQSKVSMSVYALLILTVTLSCCLFTMLCRIYKTTTVALCIQVFFIIGISPLIWFFGKSIVTYFL
VAFSLGICATPLIAVIMLLFPANVRQTGYSVAFSLAVGCFSMLTPAVLLWLEKFSIPPVLCIDFYALLSLYGFYCLKKDK
KVAEYESVFV
>Mature_409_residues
TTSNLKPMLSVLLCAFIECYDFLVYGNFSRIFSQMFFSHLTEGFALLLSFMTFGIAFFVRPFGSLLFGYVGDKYGRKIAL
FSSATLLIVSVGGIAFLPLFESIGVLSPILLIVFRILQGLSFAGEIGSVVLMAENVRDRRNIAYAMGSHFLVSIFGGAIG
CFVFKLCYNLIPEAQFYSWGWRIPFIIGLIMSLLLPLLRTSVEESREYLDYKSKRKISRMPILDVIFNHKKPFIVACIFV
PFCNSLFYTFFVFLDVQSKVSMSVYALLILTVTLSCCLFTMLCRIYKTTTVALCIQVFFIIGISPLIWFFGKSIVTYFLV
AFSLGICATPLIAVIMLLFPANVRQTGYSVAFSLAVGCFSMLTPAVLLWLEKFSIPPVLCIDFYALLSLYGFYCLKKDKK
VAEYESVFV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=234, Percent_Identity=30.7692307692308, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790550, Length=395, Percent_Identity=26.5822784810127, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789941, Length=395, Percent_Identity=28.6075949367089, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788942, Length=401, Percent_Identity=25.9351620947631, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87082231, Length=259, Percent_Identity=25.4826254826255, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46177; Mature: 46046

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure 
TTSNLKPMLSVLLCAFIECYDFLVYGNFSRIFSQMFFSHLTEGFALLLSFMTFGIAFFV
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RPFGSLLFGYVGDKYGRKIALFSSATLLIVSVGGIAFLPLFESIGVLSPILLIVFRILQG
HHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LSFAGEIGSVVLMAENVRDRRNIAYAMGSHFLVSIFGGAIGCFVFKLCYNLIPEAQFYSW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
GWRIPFIIGLIMSLLLPLLRTSVEESREYLDYKSKRKISRMPILDVIFNHKKPFIVACIF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHCCCCCHHHHHHH
VPFCNSLFYTFFVFLDVQSKVSMSVYALLILTVTLSCCLFTMLCRIYKTTTVALCIQVFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGISPLIWFFGKSIVTYFLVAFSLGICATPLIAVIMLLFPANVRQTGYSVAFSLAVGCF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
SMLTPAVLLWLEKFSIPPVLCIDFYALLSLYGFYCLKKDKKVAEYESVFV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]