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The map label for this gene is proP [H]

Identifier: 88657929

GI number: 88657929

Start: 830887

End: 832128

Strand: Reverse

Name: proP [H]

Synonym: ECH_0818

Alternate gene names: 88657929

Gene position: 832128-830887 (Counterclockwise)

Preceding gene: 88658247

Following gene: 88658389

Centisome position: 70.74

GC content: 30.19

Gene sequence:

>1242_bases
ATGTCACATGGTTCTAGTAAAAAAGCAATAATATCTGTATTTTTGTGTGCTTTTATAGAGTGTTATGATTTTTTAGTATA
TGGAAATTTTGGCCATATATTCAGTCAAATGTTTTTTTCTCAAGTCAATAGTGAGAATTTGTCATTAATATTATCGTTTG
CTACATTTGCAATAGCTTTTCTTGTTAGACCTTTCGGCTCATTGTTATTTGGGTATATTGGAGATAAGTATGGGAGAAAG
ATTTCTTTGTTTATTTCTGCAACCTTGCTTATTTTATCGGTTGGAGGTATTGCATTTTTACCTCTTGTTAGTTCCATAGG
TGTAATATCTCCTATTTTATTGGTGGTACTGCGCATTTTTCAGGGGTTATCTTTTGGAGGTGAAGTAGGTGCTATTGTGT
TAATAGCAGAAAGTGTTAGGAAAGATCAAGTTGTTGCAGTATTAAGCCTACATTTTGCAATAGCAATACTTGGAGGAGCA
ACTGGGTCTTTTATTTTTCAGTTGTGTTATAACTTTATTCCGGAAGCTCAATTTTACTCTTGGGGGTGGAGAATACCATT
TATTATTGGTTTGGTAATGTCTATGATGTTGCCTATTTTACGTAACTCTGTTGAAGAAAGTAAACAATATTTAGAGTATA
CATCTGGTAAAAAAGTTTCTAAAGTTCCTATTTTAGATATCATATTACGGCATAAAAAGATTTCTGTAATTACATTTAGT
TTAATAGCAGCTTCTAATGCTTTATTTTATGTATTTTTTGTATTTTTAAATATACAGCAGAAAGTAAGTATACCAATTTA
TAGTCTTTTAATATTTACAATGTTGGTATCAGGTTTTATATCTTTTCTTTTTTTTCGGGTATACAAACCAGAAAATGTAT
CATTGGTAATTCAAGTTTTATTTATTGTATGTGTATCTCCTGTTGTGTATTTATTAGGGCTTAACTCTATAGTTAGTTAT
TTTATATTAGCAATTGTATTAGGTATGTATGCAACTCCTATCTTATCTATATTGATATTTTTATTTCCGGTTAATATTAG
ACAAACTGGTTTTTCAATGTGTTATAGTGTTGCAGCAGGCCTTGGTGGTGCTACTCCTGCAGTTTGTTTATGGTTAATAG
AATTAACTGGATGTAGCATGTCTCCTATATTTTATTTAGATTTTTATGTGTTGTTCTCTTTACTTGGTCTTTTGTGTTTG
AAACGATATAATGAAAAGCAATGTGAAGTAGCATATGCATAA

Upstream 100 bases:

>100_bases
AGTAAGGATATATCCTAAAATCAAGTATTGTTAATAGTGATTGACACAGAGAATAAATGAGGTTATATCCTTTTTATTCT
TGTTATAATTAGTGTCTAAA

Downstream 100 bases:

>100_bases
TCAGAGTTAGATAATGTTGTACTTTATTTTATAAATTTCACAAATGATGAGTACTTATTAGTAACATGTATGTACTTAAT
AAAATGCCATAACACTGGCT

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSHGSSKKAIISVFLCAFIECYDFLVYGNFGHIFSQMFFSQVNSENLSLILSFATFAIAFLVRPFGSLLFGYIGDKYGRK
ISLFISATLLILSVGGIAFLPLVSSIGVISPILLVVLRIFQGLSFGGEVGAIVLIAESVRKDQVVAVLSLHFAIAILGGA
TGSFIFQLCYNFIPEAQFYSWGWRIPFIIGLVMSMMLPILRNSVEESKQYLEYTSGKKVSKVPILDIILRHKKISVITFS
LIAASNALFYVFFVFLNIQQKVSIPIYSLLIFTMLVSGFISFLFFRVYKPENVSLVIQVLFIVCVSPVVYLLGLNSIVSY
FILAIVLGMYATPILSILIFLFPVNIRQTGFSMCYSVAAGLGGATPAVCLWLIELTGCSMSPIFYLDFYVLFSLLGLLCL
KRYNEKQCEVAYA

Sequences:

>Translated_413_residues
MSHGSSKKAIISVFLCAFIECYDFLVYGNFGHIFSQMFFSQVNSENLSLILSFATFAIAFLVRPFGSLLFGYIGDKYGRK
ISLFISATLLILSVGGIAFLPLVSSIGVISPILLVVLRIFQGLSFGGEVGAIVLIAESVRKDQVVAVLSLHFAIAILGGA
TGSFIFQLCYNFIPEAQFYSWGWRIPFIIGLVMSMMLPILRNSVEESKQYLEYTSGKKVSKVPILDIILRHKKISVITFS
LIAASNALFYVFFVFLNIQQKVSIPIYSLLIFTMLVSGFISFLFFRVYKPENVSLVIQVLFIVCVSPVVYLLGLNSIVSY
FILAIVLGMYATPILSILIFLFPVNIRQTGFSMCYSVAAGLGGATPAVCLWLIELTGCSMSPIFYLDFYVLFSLLGLLCL
KRYNEKQCEVAYA
>Mature_412_residues
SHGSSKKAIISVFLCAFIECYDFLVYGNFGHIFSQMFFSQVNSENLSLILSFATFAIAFLVRPFGSLLFGYIGDKYGRKI
SLFISATLLILSVGGIAFLPLVSSIGVISPILLVVLRIFQGLSFGGEVGAIVLIAESVRKDQVVAVLSLHFAIAILGGAT
GSFIFQLCYNFIPEAQFYSWGWRIPFIIGLVMSMMLPILRNSVEESKQYLEYTSGKKVSKVPILDIILRHKKISVITFSL
IAASNALFYVFFVFLNIQQKVSIPIYSLLIFTMLVSGFISFLFFRVYKPENVSLVIQVLFIVCVSPVVYLLGLNSIVSYF
ILAIVLGMYATPILSILIFLFPVNIRQTGFSMCYSVAAGLGGATPAVCLWLIELTGCSMSPIFYLDFYVLFSLLGLLCLK
RYNEKQCEVAYA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=435, Percent_Identity=26.8965517241379, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1788292, Length=239, Percent_Identity=33.8912133891213, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789941, Length=394, Percent_Identity=30.2030456852792, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788942, Length=412, Percent_Identity=26.6990291262136, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87082231, Length=304, Percent_Identity=25.6578947368421, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45806; Mature: 45674

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS00185 IPNS_1 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SHGSSKKAIISVFLCAFIECYDFLVYGNFGHIFSQMFFSQVNSENLSLILSFATFAIAF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LVRPFGSLLFGYIGDKYGRKISLFISATLLILSVGGIAFLPLVSSIGVISPILLVVLRIF
HHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGLSFGGEVGAIVLIAESVRKDQVVAVLSLHFAIAILGGATGSFIFQLCYNFIPEAQFYS
HCCCCCCCCCHHEEEHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHC
WGWRIPFIIGLVMSMMLPILRNSVEESKQYLEYTSGKKVSKVPILDIILRHKKISVITFS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHH
LIAASNALFYVFFVFLNIQQKVSIPIYSLLIFTMLVSGFISFLFFRVYKPENVSLVIQVL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FIVCVSPVVYLLGLNSIVSYFILAIVLGMYATPILSILIFLFPVNIRQTGFSMCYSVAAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
LGGATPAVCLWLIELTGCSMSPIFYLDFYVLFSLLGLLCLKRYNEKQCEVAYA
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]