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The map label for this gene is folE [H]

Identifier: 88658359

GI number: 88658359

Start: 654862

End: 655434

Strand: Reverse

Name: folE [H]

Synonym: ECH_0651

Alternate gene names: 88658359

Gene position: 655434-654862 (Counterclockwise)

Preceding gene: 88658663

Following gene: 88657647

Centisome position: 55.72

GC content: 30.54

Gene sequence:

>573_bases
ATGAGAAATGGCAAACCTTCTGATAGTGAAGCCGAAGAAGCTGTTAATTTACTAATAAGATGGATAGGTGATGATCCAAA
TAGAGAAGGTCTTGTTAATACTGCTAAACGTGTATTGGATGTCTATAAAAGATTTTTTTCAGGATATCAAGTAGATATAT
ATTCTATGCAAGATTTAGTTTTACTTAATGAGAATTATAATGATATGGTGATTTTTAAAAATACTGAATTTACATCGTAT
TGTGAGCATCATATTGTTCCAATGAGAGGTAAAATTAGTATTGGGTATGTTCCTGATAAGTTAGTATTTGGAGTAGGTAA
AATTGTAAAATTAATTAACTGTTTTACAAAAAGATTGCAACTTCAAGAGAAATTAACTGTAGAAATAGCAAACGCCTTAG
ATCATTGTTTAGCTCCGAAAGGGATAATTGTTGCTGTTGAAGCAGTACATGATTGTATTGTATGCTGTGAAGAGGAAGAC
AAGCATAATTTGGAATTACAAACGACTTGTGCAATTGGAGTTTTTCAAGATAACATGGAATTAAGAAGAGAATTTTTTTC
TAGTATTGGTTGA

Upstream 100 bases:

>100_bases
TTGGCATTAGAATATAATCGTTCTAGGCAGGCTTCTATAACTACTGACTTAATAGAGATAATTAGTGGTTTTGAATCGTT
GAATTAATAAGTGAGAATTT

Downstream 100 bases:

>100_bases
TGGAGGATAAATTGGAGATTTTAAATGTAGCTGAAGATGCTGTAAAAAAGATAAAAAGTATGGGTTACATTGGTGATGTA
GTAATTTATCAGAATAATAG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MRNGKPSDSEAEEAVNLLIRWIGDDPNREGLVNTAKRVLDVYKRFFSGYQVDIYSMQDLVLLNENYNDMVIFKNTEFTSY
CEHHIVPMRGKISIGYVPDKLVFGVGKIVKLINCFTKRLQLQEKLTVEIANALDHCLAPKGIIVAVEAVHDCIVCCEEED
KHNLELQTTCAIGVFQDNMELRREFFSSIG

Sequences:

>Translated_190_residues
MRNGKPSDSEAEEAVNLLIRWIGDDPNREGLVNTAKRVLDVYKRFFSGYQVDIYSMQDLVLLNENYNDMVIFKNTEFTSY
CEHHIVPMRGKISIGYVPDKLVFGVGKIVKLINCFTKRLQLQEKLTVEIANALDHCLAPKGIIVAVEAVHDCIVCCEEED
KHNLELQTTCAIGVFQDNMELRREFFSSIG
>Mature_190_residues
MRNGKPSDSEAEEAVNLLIRWIGDDPNREGLVNTAKRVLDVYKRFFSGYQVDIYSMQDLVLLNENYNDMVIFKNTEFTSY
CEHHIVPMRGKISIGYVPDKLVFGVGKIVKLINCFTKRLQLQEKLTVEIANALDHCLAPKGIIVAVEAVHDCIVCCEEED
KHNLELQTTCAIGVFQDNMELRREFFSSIG

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=176, Percent_Identity=36.9318181818182, Blast_Score=137, Evalue=8e-33,
Organism=Homo sapiens, GI4503949, Length=176, Percent_Identity=36.9318181818182, Blast_Score=137, Evalue=8e-33,
Organism=Homo sapiens, GI66932972, Length=136, Percent_Identity=38.2352941176471, Blast_Score=117, Evalue=7e-27,
Organism=Homo sapiens, GI66932970, Length=136, Percent_Identity=38.2352941176471, Blast_Score=116, Evalue=1e-26,
Organism=Escherichia coli, GI1788476, Length=178, Percent_Identity=29.7752808988764, Blast_Score=84, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17560486, Length=176, Percent_Identity=38.0681818181818, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6321706, Length=180, Percent_Identity=38.3333333333333, Blast_Score=130, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24656782, Length=188, Percent_Identity=35.6382978723404, Blast_Score=123, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24656791, Length=188, Percent_Identity=35.6382978723404, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24656796, Length=188, Percent_Identity=35.6382978723404, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 21663; Mature: 21663

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNGKPSDSEAEEAVNLLIRWIGDDPNREGLVNTAKRVLDVYKRFFSGYQVDIYSMQDLV
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEEE
LLNENYNDMVIFKNTEFTSYCEHHIVPMRGKISIGYVPDKLVFGVGKIVKLINCFTKRLQ
EEECCCCEEEEEECCCHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LQEKLTVEIANALDHCLAPKGIIVAVEAVHDCIVCCEEEDKHNLELQTTCAIGVFQDNME
HHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHEECCCCCCCCCEEEEEEEEEEECCCHH
LRREFFSSIG
HHHHHHHHCC
>Mature Secondary Structure
MRNGKPSDSEAEEAVNLLIRWIGDDPNREGLVNTAKRVLDVYKRFFSGYQVDIYSMQDLV
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCEEE
LLNENYNDMVIFKNTEFTSYCEHHIVPMRGKISIGYVPDKLVFGVGKIVKLINCFTKRLQ
EEECCCCEEEEEECCCHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
LQEKLTVEIANALDHCLAPKGIIVAVEAVHDCIVCCEEEDKHNLELQTTCAIGVFQDNME
HHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHEECCCCCCCCCEEEEEEEEEEECCCHH
LRREFFSSIG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA