Click here to switch to the map view.

The map label for this gene is pmbA [H]

Identifier: 88657647

GI number: 88657647

Start: 653516

End: 654862

Strand: Reverse

Name: pmbA [H]

Synonym: ECH_0650

Alternate gene names: 88657647

Gene position: 654862-653516 (Counterclockwise)

Preceding gene: 88658359

Following gene: 88657729

Centisome position: 55.67

GC content: 29.47

Gene sequence:

>1347_bases
ATGGAGGATAAATTGGAGATTTTAAATGTAGCTGAAGATGCTGTAAAAAAGATAAAAAGTATGGGTTACATTGGTGATGT
AGTAATTTATCAGAATAATAGGTTATCTTTATCGCAACGTCTCCATAAGATTGATGAAGTTATTCAATCTAAAAACTGTA
AAATGGGAATAAGAGTTATTGTGAATAATAATCAATTTGCATGTGTTTCTTCTAATGAACTTTCTAATGTATCAGAATCT
ATAGATCAAGCTGTTGCTATGGCAAAATTATCTACAGGAGATCCATATATTTCTTTATATAATGATTTTATTAATAAAGA
ACATTTGTTAACTAGTAATGACCTAATGATATTAGATAAGACTAATATTACAGTTGATCAATGTAAAAAAATTGTTGAAG
ATATGGAAAATGCTGCGTTATCTTATGATAATCAAGTTGTCAATTCAGAAGGATCTTCATTTTCTCATGGTATAACAGAT
GTTGTTTTAGCAACTACAAATGGTTTTATAGGTTCTTATAGTAAATCTAATTTTGTAAGTAGTATTTCTGTTATTGCTGG
TACAGATGATAAAATGGAAGTAGGATATGATTTTTCTTCCGTATGCAACTTAAGTCGTATGAAAGATCCTGTAAAAATAG
GTAAGGAAGCTGCATTAAGAGCAGTTAAGAGATTAAACCCTGGAAAATTACAGACCTGTAAAGTACCAATCGTGATTGAG
AATAGGGTAGCTGGTACATTGTTAAAAAACTTTGCCAGTGCTATAAGGGGGGATAATATAGCTAATGAAGCTTCATTTTT
AAATAATTATATTAATAAAAAGATTTTTTCAGATGACATTTTTATAGTAGATGATCCATTAATGTTATCTGGCCTATCTT
CAAGGCCTTTTGATGGAGAAGGAGTTGTTGGTAATAAAAAGTTAGTAGTAGAAAATGGTATATTAAAAAGTTGGATTTTA
GATATACGTTCAGCTAATAAGTTGAAACTGAAAACTACTGGAAATGCTGTAAGGCATTCTAATGCATCAGTAAGTCCTGG
TGTTAGCAATTTTTATATACAGAATGGAATTTTGTCTGTTCAAGAGTTAATCTCAGATATAAAAAGTGGGCTTTATATTA
CTGATCTTTTTGGTTTTGGAGTAAATTTAATTACTGGTGATTATAGTCAAGGTGCATCAGGATTTTTTATAGAGAATGGT
AAAATATCTTATCCAGTAAATGAAATCACCATTGCTAGTAAGTTACAATATATGTTTTCTGAAATGTCCATTGCAAATGA
TTTGGTATTTTTTAGTTCAGTCAATTCTCCTACAATAAGAATAGATGGCATTACAATTGCTGGATAA

Upstream 100 bases:

>100_bases
AGGAAGACAAGCATAATTTGGAATTACAAACGACTTGTGCAATTGGAGTTTTTCAAGATAACATGGAATTAAGAAGAGAA
TTTTTTTCTAGTATTGGTTG

Downstream 100 bases:

>100_bases
TAAAATAAATAGTGTTGTTCTATATGTTTTAGGTAAATTGTTATGATTTACTATGTATTTGTTATTGTGGTGCTTAATGT
TATATTTATTAGAATATACT

Product: pmbA protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MEDKLEILNVAEDAVKKIKSMGYIGDVVIYQNNRLSLSQRLHKIDEVIQSKNCKMGIRVIVNNNQFACVSSNELSNVSES
IDQAVAMAKLSTGDPYISLYNDFINKEHLLTSNDLMILDKTNITVDQCKKIVEDMENAALSYDNQVVNSEGSSFSHGITD
VVLATTNGFIGSYSKSNFVSSISVIAGTDDKMEVGYDFSSVCNLSRMKDPVKIGKEAALRAVKRLNPGKLQTCKVPIVIE
NRVAGTLLKNFASAIRGDNIANEASFLNNYINKKIFSDDIFIVDDPLMLSGLSSRPFDGEGVVGNKKLVVENGILKSWIL
DIRSANKLKLKTTGNAVRHSNASVSPGVSNFYIQNGILSVQELISDIKSGLYITDLFGFGVNLITGDYSQGASGFFIENG
KISYPVNEITIASKLQYMFSEMSIANDLVFFSSVNSPTIRIDGITIAG

Sequences:

>Translated_448_residues
MEDKLEILNVAEDAVKKIKSMGYIGDVVIYQNNRLSLSQRLHKIDEVIQSKNCKMGIRVIVNNNQFACVSSNELSNVSES
IDQAVAMAKLSTGDPYISLYNDFINKEHLLTSNDLMILDKTNITVDQCKKIVEDMENAALSYDNQVVNSEGSSFSHGITD
VVLATTNGFIGSYSKSNFVSSISVIAGTDDKMEVGYDFSSVCNLSRMKDPVKIGKEAALRAVKRLNPGKLQTCKVPIVIE
NRVAGTLLKNFASAIRGDNIANEASFLNNYINKKIFSDDIFIVDDPLMLSGLSSRPFDGEGVVGNKKLVVENGILKSWIL
DIRSANKLKLKTTGNAVRHSNASVSPGVSNFYIQNGILSVQELISDIKSGLYITDLFGFGVNLITGDYSQGASGFFIENG
KISYPVNEITIASKLQYMFSEMSIANDLVFFSSVNSPTIRIDGITIAG
>Mature_448_residues
MEDKLEILNVAEDAVKKIKSMGYIGDVVIYQNNRLSLSQRLHKIDEVIQSKNCKMGIRVIVNNNQFACVSSNELSNVSES
IDQAVAMAKLSTGDPYISLYNDFINKEHLLTSNDLMILDKTNITVDQCKKIVEDMENAALSYDNQVVNSEGSSFSHGITD
VVLATTNGFIGSYSKSNFVSSISVIAGTDDKMEVGYDFSSVCNLSRMKDPVKIGKEAALRAVKRLNPGKLQTCKVPIVIE
NRVAGTLLKNFASAIRGDNIANEASFLNNYINKKIFSDDIFIVDDPLMLSGLSSRPFDGEGVVGNKKLVVENGILKSWIL
DIRSANKLKLKTTGNAVRHSNASVSPGVSNFYIQNGILSVQELISDIKSGLYITDLFGFGVNLITGDYSQGASGFFIENG
KISYPVNEITIASKLQYMFSEMSIANDLVFFSSVNSPTIRIDGITIAG

Specific function: May Facilitate The Secretion Of The Peptide Antibiotic Microcin B17 (Mccb17) By Completing Its Maturation. Suppresses The Inhibitory Activity Of The Carbon Storage Regulator (Csra). [C]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=427, Percent_Identity=31.615925058548, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789640, Length=252, Percent_Identity=27.7777777777778, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48938; Mature: 48938

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDKLEILNVAEDAVKKIKSMGYIGDVVIYQNNRLSLSQRLHKIDEVIQSKNCKMGIRVI
CCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEE
VNNNQFACVSSNELSNVSESIDQAVAMAKLSTGDPYISLYNDFINKEHLLTSNDLMILDK
EECCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEECCCEEEEEC
TNITVDQCKKIVEDMENAALSYDNQVVNSEGSSFSHGITDVVLATTNGFIGSYSKSNFVS
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHH
SISVIAGTDDKMEVGYDFSSVCNLSRMKDPVKIGKEAALRAVKRLNPGKLQTCKVPIVIE
EEEEEECCCCCEEECCCHHHHHHHHHCCCHHHHCHHHHHHHHHHCCCCCCEEEEEEEEEC
NRVAGTLLKNFASAIRGDNIANEASFLNNYINKKIFSDDIFIVDDPLMLSGLSSRPFDGE
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCEEEEECCHHCCCCCCCCCCCC
GVVGNKKLVVENGILKSWILDIRSANKLKLKTTGNAVRHSNASVSPGVSNFYIQNGILSV
CCCCCCEEEEECCHHHHHHHHHCCCCEEEEEECCCHHCCCCCCCCCCCCCEEEECCHHHH
QELISDIKSGLYITDLFGFGVNLITGDYSQGASGFFIENGKISYPVNEITIASKLQYMFS
HHHHHHHHCCEEEEEHHHCCEEEEECCCCCCCCEEEEECCEEECCHHHHHHHHHHHHHHH
EMSIANDLVFFSSVNSPTIRIDGITIAG
HHHHHHCEEEEECCCCCEEEEECEEECC
>Mature Secondary Structure
MEDKLEILNVAEDAVKKIKSMGYIGDVVIYQNNRLSLSQRLHKIDEVIQSKNCKMGIRVI
CCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEE
VNNNQFACVSSNELSNVSESIDQAVAMAKLSTGDPYISLYNDFINKEHLLTSNDLMILDK
EECCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEECCCEEEEEC
TNITVDQCKKIVEDMENAALSYDNQVVNSEGSSFSHGITDVVLATTNGFIGSYSKSNFVS
CCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHH
SISVIAGTDDKMEVGYDFSSVCNLSRMKDPVKIGKEAALRAVKRLNPGKLQTCKVPIVIE
EEEEEECCCCCEEECCCHHHHHHHHHCCCHHHHCHHHHHHHHHHCCCCCCEEEEEEEEEC
NRVAGTLLKNFASAIRGDNIANEASFLNNYINKKIFSDDIFIVDDPLMLSGLSSRPFDGE
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCEEEEECCHHCCCCCCCCCCCC
GVVGNKKLVVENGILKSWILDIRSANKLKLKTTGNAVRHSNASVSPGVSNFYIQNGILSV
CCCCCCEEEEECCHHHHHHHHHCCCCEEEEEECCCHHCCCCCCCCCCCCCEEEECCHHHH
QELISDIKSGLYITDLFGFGVNLITGDYSQGASGFFIENGKISYPVNEITIASKLQYMFS
HHHHHHHHCCEEEEEHHHCCEEEEECCCCCCCCEEEEECCEEECCHHHHHHHHHHHHHHH
EMSIANDLVFFSSVNSPTIRIDGITIAG
HHHHHHCEEEEECCCCCEEEEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]