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The map label for this gene is tme [H]

Identifier: 88658354

GI number: 88658354

Start: 164885

End: 167161

Strand: Direct

Name: tme [H]

Synonym: ECH_0175

Alternate gene names: 88658354

Gene position: 164885-167161 (Clockwise)

Preceding gene: 88658163

Following gene: 88658243

Centisome position: 14.02

GC content: 34.48

Gene sequence:

>2277_bases
ATGGTAGATCTTGAAAATAACGGTTTTGATGAAAAGAGAAAAGAAGCTCTTGAATTCCATTGTAAGGATTTAAAACCTGG
AAAAGTTTCTCTGTTACCCACTAAACCTCTATTAACACAAAGAGACTTATCATTAGCATATTCTCCTGGTGTTGCAGTAC
CTTGTTTAGAAATAGCTAGAAATCCTGATTTAGTATATGAATATACTGCGCGAGGAAATTATGTTGCTGTCATTTCAAAT
GGTACAGCTGTGCTTGGATTAGGTAATATAGGGCCTTTAGCATCTAAACCTGTTATGGAAGGTAAAGCTGTTTTATTTAA
ACGGTTTGCTGATATTGATGCAGTGGATATAGAAGTTAACACTTCTGATGTAGATGAATTTGTTAATACTGTAAAATATT
TGGGACTAAGTTGGGGAGGCATAAATTTAGAAGATATAAAAGCTCCTGAGTGTTTTATTATTGAAAAAAGATTGAGTGAA
ATAATGGATATTCCAGTGTTCCATGATGATCAACATGGAACTGCTATTATTGTGGCTGCTGGACTTATTAATGCTCTTGA
TATTACAGGTAAAAGTTTTAAAGATGTTAAAATAGTTATAAATGGTGCTGGTGCTGCAGGTATTGCATGTCTTGAAATGA
TAAAGTTAATAGGAGTTCCTGCTGAGAATATAACTTTATGTGACCAAAATGGGGTAATATATAAAGGTAGACAACTTGGC
ATGAATGAGTGGAAAGAAAAGCATGCGATTGAAACAGAAAATAGGTCTTTGAAAGATGCATTGATAATGGCTGATGTATT
TCTTGGGTTATCAGTTAAGGATGTGTTGAGTAAAGATATGCTGTTGTCTATGAACAGAGATCCTGTCATTTTTGCTCTAG
CAAATCCAGATCCTGAAATTAATCCTAATGTTGCACATGAGATTCGACCTGATGCTATTATAGCTACGGGAAGATCAGAT
TATAATAATCAGATTAATAATGTAATGGGATTCCCTTATATATTTCGTGGTGCGTTAGATGTACGTGCTAAATCAGTTAA
CAATGAGATGAAAATAGCTGCAGCAAATGCAATTGCTATGTTAGCGCGTGAATATGTTAGTGATGAAGTGTCTGATGCAT
ATGGTGGTCGTAAAATGAATTATGGGAAGGATTATATTATACCTACTCCATTTGATCCTAGATTAATTACTGTAGTATCT
CCTGCAGTTGCAAAAGCTGCTATTGATTCTGGAGTTGCTAGAAAAAATATAGAGAATTGGGATGAATATACTAAACAGTT
AGCATCACGACTATCATTGACTTCTAATATCCTTAGTATGATGTACAGTGCTGTTAAATGTGATCCTAAAAGAGTTATTT
TTTCGGAAGGAGAAGAAGAAAAAGTTATTGAGGCTGCTGTACAGTGGCGTAATCAAGAATATGGATTACCTATACTTGTA
GGGCGTATCGACAAAGTGCAAGAAGCATTTGACCGTTTAGGTATTAAAGATACAAAAGGTATAGAGATTGCTAATGCAGC
AATTTCTCAACGAAATAATGAGTATACAGACTATTTATATAAGAAATTACAGCGTGAAGGATATTTATACAGGAGTTGTG
TACGAGATGTAAAAACAGATCGTAATGTTTTTGCTGCGTGTATGCTTGCATGTGGTGATGGTGATGTGTTGATTACTGGA
ATGACAAGGGGGTATTATGCTTCTATTAATGATGTACAGAAAGTCATAGGTTCTCAAGGTGTCGTATTTGGATTATCAAT
AATTGTTATGAAGGAGCGTACTATATTTGTTGCAGATACTGCTATACATGAATCTCCAACTCCAGAGCAAATTGCTGAGA
TAGCAATTCAGGCATCTGTTCAAGCTAAGAAAATGGGGTATGAACCTCGTGTTAGCTTTATTTCTTCTTCAAACTTTGGT
AGTCATTCTCAAGAAGATGCTAAGAAAATGAGACAAGCTATAAAAATTTTGGATAGTTATAATGTTAGCTTTGAATATGA
TGGAGAAATGTCTGTTGATACTGCGCTTAATCCAGAGTTGTTAACATTATACCCTTTCTGTAAGTTAACTGGTGAAGCAA
ATGTTTTAGTGATGCCAACTCTTCATAGTGCTAGTATATCTTCTAAGTTATTACAGAGGGCTGCTGGAGTATCTGTAATA
GGTCCTATTTTAATTGGAATGGAAAAGCCTGTACAAATTGTACAAATGTCTTCTTCTGTGTCTGAGATTCTTAATCTTAC
TGTACTTGCATCTTTAGTGAATAATACTAATTTTTAA

Upstream 100 bases:

>100_bases
ATTAATAATGTATCATGATGTTTGTTAAAAGCTTGACATAGCGTGTTATCTGGCAGTATACAAGTGAGGCTTTAATAGAA
GTGTTTATTGAGTATGTTTT

Downstream 100 bases:

>100_bases
TCTTCTTGTAAATAATTGCGTGTATATAAGATTATGAATTTGTATTAATATATATAACTGTATTAATTATTTATTAAGCA
TCGCTATTTATTGGGTTATA

Product: malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 758; Mature: 758

Protein sequence:

>758_residues
MVDLENNGFDEKRKEALEFHCKDLKPGKVSLLPTKPLLTQRDLSLAYSPGVAVPCLEIARNPDLVYEYTARGNYVAVISN
GTAVLGLGNIGPLASKPVMEGKAVLFKRFADIDAVDIEVNTSDVDEFVNTVKYLGLSWGGINLEDIKAPECFIIEKRLSE
IMDIPVFHDDQHGTAIIVAAGLINALDITGKSFKDVKIVINGAGAAGIACLEMIKLIGVPAENITLCDQNGVIYKGRQLG
MNEWKEKHAIETENRSLKDALIMADVFLGLSVKDVLSKDMLLSMNRDPVIFALANPDPEINPNVAHEIRPDAIIATGRSD
YNNQINNVMGFPYIFRGALDVRAKSVNNEMKIAAANAIAMLAREYVSDEVSDAYGGRKMNYGKDYIIPTPFDPRLITVVS
PAVAKAAIDSGVARKNIENWDEYTKQLASRLSLTSNILSMMYSAVKCDPKRVIFSEGEEEKVIEAAVQWRNQEYGLPILV
GRIDKVQEAFDRLGIKDTKGIEIANAAISQRNNEYTDYLYKKLQREGYLYRSCVRDVKTDRNVFAACMLACGDGDVLITG
MTRGYYASINDVQKVIGSQGVVFGLSIIVMKERTIFVADTAIHESPTPEQIAEIAIQASVQAKKMGYEPRVSFISSSNFG
SHSQEDAKKMRQAIKILDSYNVSFEYDGEMSVDTALNPELLTLYPFCKLTGEANVLVMPTLHSASISSKLLQRAAGVSVI
GPILIGMEKPVQIVQMSSSVSEILNLTVLASLVNNTNF

Sequences:

>Translated_758_residues
MVDLENNGFDEKRKEALEFHCKDLKPGKVSLLPTKPLLTQRDLSLAYSPGVAVPCLEIARNPDLVYEYTARGNYVAVISN
GTAVLGLGNIGPLASKPVMEGKAVLFKRFADIDAVDIEVNTSDVDEFVNTVKYLGLSWGGINLEDIKAPECFIIEKRLSE
IMDIPVFHDDQHGTAIIVAAGLINALDITGKSFKDVKIVINGAGAAGIACLEMIKLIGVPAENITLCDQNGVIYKGRQLG
MNEWKEKHAIETENRSLKDALIMADVFLGLSVKDVLSKDMLLSMNRDPVIFALANPDPEINPNVAHEIRPDAIIATGRSD
YNNQINNVMGFPYIFRGALDVRAKSVNNEMKIAAANAIAMLAREYVSDEVSDAYGGRKMNYGKDYIIPTPFDPRLITVVS
PAVAKAAIDSGVARKNIENWDEYTKQLASRLSLTSNILSMMYSAVKCDPKRVIFSEGEEEKVIEAAVQWRNQEYGLPILV
GRIDKVQEAFDRLGIKDTKGIEIANAAISQRNNEYTDYLYKKLQREGYLYRSCVRDVKTDRNVFAACMLACGDGDVLITG
MTRGYYASINDVQKVIGSQGVVFGLSIIVMKERTIFVADTAIHESPTPEQIAEIAIQASVQAKKMGYEPRVSFISSSNFG
SHSQEDAKKMRQAIKILDSYNVSFEYDGEMSVDTALNPELLTLYPFCKLTGEANVLVMPTLHSASISSKLLQRAAGVSVI
GPILIGMEKPVQIVQMSSSVSEILNLTVLASLVNNTNF
>Mature_758_residues
MVDLENNGFDEKRKEALEFHCKDLKPGKVSLLPTKPLLTQRDLSLAYSPGVAVPCLEIARNPDLVYEYTARGNYVAVISN
GTAVLGLGNIGPLASKPVMEGKAVLFKRFADIDAVDIEVNTSDVDEFVNTVKYLGLSWGGINLEDIKAPECFIIEKRLSE
IMDIPVFHDDQHGTAIIVAAGLINALDITGKSFKDVKIVINGAGAAGIACLEMIKLIGVPAENITLCDQNGVIYKGRQLG
MNEWKEKHAIETENRSLKDALIMADVFLGLSVKDVLSKDMLLSMNRDPVIFALANPDPEINPNVAHEIRPDAIIATGRSD
YNNQINNVMGFPYIFRGALDVRAKSVNNEMKIAAANAIAMLAREYVSDEVSDAYGGRKMNYGKDYIIPTPFDPRLITVVS
PAVAKAAIDSGVARKNIENWDEYTKQLASRLSLTSNILSMMYSAVKCDPKRVIFSEGEEEKVIEAAVQWRNQEYGLPILV
GRIDKVQEAFDRLGIKDTKGIEIANAAISQRNNEYTDYLYKKLQREGYLYRSCVRDVKTDRNVFAACMLACGDGDVLITG
MTRGYYASINDVQKVIGSQGVVFGLSIIVMKERTIFVADTAIHESPTPEQIAEIAIQASVQAKKMGYEPRVSFISSSNFG
SHSQEDAKKMRQAIKILDSYNVSFEYDGEMSVDTALNPELLTLYPFCKLTGEANVLVMPTLHSASISSKLLQRAAGVSVI
GPILIGMEKPVQIVQMSSSVSEILNLTVLASLVNNTNF

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=407, Percent_Identity=25.0614250614251, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788806, Length=756, Percent_Identity=45.7671957671958, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1788800, Length=323, Percent_Identity=28.1733746130031, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788635, Length=307, Percent_Identity=26.3843648208469, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6322823, Length=235, Percent_Identity=28.5106382978723, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI78707242, Length=447, Percent_Identity=25.0559284116331, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI78707238, Length=447, Percent_Identity=25.0559284116331, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281362674, Length=396, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281362672, Length=397, Percent_Identity=27.455919395466, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356279, Length=397, Percent_Identity=27.455919395466, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78707240, Length=433, Percent_Identity=24.7113163972286, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 83202; Mature: 83202

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDLENNGFDEKRKEALEFHCKDLKPGKVSLLPTKPLLTQRDLSLAYSPGVAVPCLEIAR
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEECCCCCCHHHHHCC
NPDLVYEYTARGNYVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKRFADIDAVDIEVN
CCCEEEEEECCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEC
TSDVDEFVNTVKYLGLSWGGINLEDIKAPECFIIEKRLSEIMDIPVFHDDQHGTAIIVAA
HHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEEEEHHHHHHHHCCCCEECCCCCCEEEEEE
GLINALDITGKSFKDVKIVINGAGAAGIACLEMIKLIGVPAENITLCDQNGVIYKGRQLG
HHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECHHCC
MNEWKEKHAIETENRSLKDALIMADVFLGLSVKDVLSKDMLLSMNRDPVIFALANPDPEI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHEECCCCCEEEEECCCCCCC
NPNVAHEIRPDAIIATGRSDYNNQINNVMGFPYIFRGALDVRAKSVNNEMKIAAANAIAM
CCCCHHHCCCCEEEEECCCCHHHHHHHCCCCCHHHHCHHHHHHHHCCCCEEEEHHHHHHH
LAREYVSDEVSDAYGGRKMNYGKDYIIPTPFDPRLITVVSPAVAKAAIDSGVARKNIENW
HHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCH
DEYTKQLASRLSLTSNILSMMYSAVKCDPKRVIFSEGEEEKVIEAAVQWRNQEYGLPILV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEE
GRIDKVQEAFDRLGIKDTKGIEIANAAISQRNNEYTDYLYKKLQREGYLYRSCVRDVKTD
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHCC
RNVFAACMLACGDGDVLITGMTRGYYASINDVQKVIGSQGVVFGLSIIVMKERTIFVADT
HHHHHHHHHEECCCCEEEEECCCCEEECHHHHHHHHCCCCCEEEEEEEEEECCEEEEEEC
AIHESPTPEQIAEIAIQASVQAKKMGYEPRVSFISSSNFGSHSQEDAKKMRQAIKILDSY
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
NVSFEYDGEMSVDTALNPELLTLYPFCKLTGEANVLVMPTLHSASISSKLLQRAAGVSVI
CCEEEECCCEEECCCCCCCEEEEEHHHEECCCCCEEEEECCCHHHHHHHHHHHHCCCHHH
GPILIGMEKPVQIVQMSSSVSEILNLTVLASLVNNTNF
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVDLENNGFDEKRKEALEFHCKDLKPGKVSLLPTKPLLTQRDLSLAYSPGVAVPCLEIAR
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEECCCCCCHHHHHCC
NPDLVYEYTARGNYVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKRFADIDAVDIEVN
CCCEEEEEECCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEC
TSDVDEFVNTVKYLGLSWGGINLEDIKAPECFIIEKRLSEIMDIPVFHDDQHGTAIIVAA
HHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEEEEHHHHHHHHCCCCEECCCCCCEEEEEE
GLINALDITGKSFKDVKIVINGAGAAGIACLEMIKLIGVPAENITLCDQNGVIYKGRQLG
HHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECHHCC
MNEWKEKHAIETENRSLKDALIMADVFLGLSVKDVLSKDMLLSMNRDPVIFALANPDPEI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHEECCCCCEEEEECCCCCCC
NPNVAHEIRPDAIIATGRSDYNNQINNVMGFPYIFRGALDVRAKSVNNEMKIAAANAIAM
CCCCHHHCCCCEEEEECCCCHHHHHHHCCCCCHHHHCHHHHHHHHCCCCEEEEHHHHHHH
LAREYVSDEVSDAYGGRKMNYGKDYIIPTPFDPRLITVVSPAVAKAAIDSGVARKNIENW
HHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCH
DEYTKQLASRLSLTSNILSMMYSAVKCDPKRVIFSEGEEEKVIEAAVQWRNQEYGLPILV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEE
GRIDKVQEAFDRLGIKDTKGIEIANAAISQRNNEYTDYLYKKLQREGYLYRSCVRDVKTD
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHCC
RNVFAACMLACGDGDVLITGMTRGYYASINDVQKVIGSQGVVFGLSIIVMKERTIFVADT
HHHHHHHHHEECCCCEEEEECCCCEEECHHHHHHHHCCCCCEEEEEEEEEECCEEEEEEC
AIHESPTPEQIAEIAIQASVQAKKMGYEPRVSFISSSNFGSHSQEDAKKMRQAIKILDSY
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
NVSFEYDGEMSVDTALNPELLTLYPFCKLTGEANVLVMPTLHSASISSKLLQRAAGVSVI
CCEEEECCCEEECCCCCCCEEEEEHHHEECCCCCEEEEECCCHHHHHHHHHHHHCCCHHH
GPILIGMEKPVQIVQMSSSVSEILNLTVLASLVNNTNF
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]