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The map label for this gene is tmk

Identifier: 88658326

GI number: 88658326

Start: 217178

End: 217786

Strand: Direct

Name: tmk

Synonym: ECH_0229

Alternate gene names: 88658326

Gene position: 217178-217786 (Clockwise)

Preceding gene: 88657851

Following gene: 88657781

Centisome position: 18.46

GC content: 28.9

Gene sequence:

>609_bases
ATGTTCATTACTTTTGAAGGAATAGATGGTTCTGGTAAAACTACACAGTCACATTTGTTGGCGGAATATTTATCTGAAAT
ATATGGTGTGAATAATGTTGTGTTAACTAGAGAACCTGGAGGTACTTTATTAAATGAATCAGTGCGCAACTTATTATTTA
AAGCTCAAGGTTTAGATAGTTTATCAGAATTATTATTTTTTATTGCTATGCGTAGGGAGCATTTTGTTAAAATTATAAAA
CCTTCTTTAATGCAAAAAAAAATAGTGATTTGTGATAGATTTATCGATTCGACAATTGCATATCAAGGTTATGGACAAGG
TATTGATTGTAGTTTGATAGATCAGTTAAATGATCTTGTTATTGATGTTTACCCTGATATTACATTTATTATTGATGTTG
ATATTAATGAATCATTGTCTAGATCTTGTAAAAATGGATATGAATTTGCTGATATGGAATTTTATTATAGAGTCAGAGAT
GGCTTCTATGACATTGCAAAAAAAAATCCGCATCGTTGTCATGTAATTACTGATAAGAGTGAAACTTATGATATTGATGA
CATTAACTTTGTACATTTGGAAGTAATAAAGGTATTGCAAATGGTATGA

Upstream 100 bases:

>100_bases
TATTGCTTACTTTATAAGGTATAAGTGACTTAGGTATTTTTACTTGCATGTACTTGAGTAATTAGTAATAAAGTTGTTTG
TATCTAATGTAAGGTAATTT

Downstream 100 bases:

>100_bases
ATTTGTGTGTAGGATCTACACCTTGTTTTTTTTTATAATTTTAATGGTTTTAAATTAATCATGGGGGATGTTTTGAATAG
AAAATTTTTAGCATGGTTTT

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MFITFEGIDGSGKTTQSHLLAEYLSEIYGVNNVVLTREPGGTLLNESVRNLLFKAQGLDSLSELLFFIAMRREHFVKIIK
PSLMQKKIVICDRFIDSTIAYQGYGQGIDCSLIDQLNDLVIDVYPDITFIIDVDINESLSRSCKNGYEFADMEFYYRVRD
GFYDIAKKNPHRCHVITDKSETYDIDDINFVHLEVIKVLQMV

Sequences:

>Translated_202_residues
MFITFEGIDGSGKTTQSHLLAEYLSEIYGVNNVVLTREPGGTLLNESVRNLLFKAQGLDSLSELLFFIAMRREHFVKIIK
PSLMQKKIVICDRFIDSTIAYQGYGQGIDCSLIDQLNDLVIDVYPDITFIIDVDINESLSRSCKNGYEFADMEFYYRVRD
GFYDIAKKNPHRCHVITDKSETYDIDDINFVHLEVIKVLQMV
>Mature_202_residues
MFITFEGIDGSGKTTQSHLLAEYLSEIYGVNNVVLTREPGGTLLNESVRNLLFKAQGLDSLSELLFFIAMRREHFVKIIK
PSLMQKKIVICDRFIDSTIAYQGYGQGIDCSLIDQLNDLVIDVYPDITFIIDVDINESLSRSCKNGYEFADMEFYYRVRD
GFYDIAKKNPHRCHVITDKSETYDIDDINFVHLEVIKVLQMV

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family

Homologues:

Organism=Escherichia coli, GI1787340, Length=178, Percent_Identity=36.5168539325843, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KTHY_EHRCR (Q2GHN3)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507050.1
- PDB:   3LD9
- PDBsum:   3LD9
- ProteinModelPortal:   Q2GHN3
- STRING:   Q2GHN3
- GeneID:   3927908
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0229
- TIGR:   ECH_0229
- eggNOG:   COG0125
- HOGENOM:   HBG626009
- OMA:   KGHIVIS
- PhylomeDB:   Q2GHN3
- ProtClustDB:   PRK13976
- BioCyc:   ECHA205920:ECH_0229-MONOMER
- HAMAP:   MF_00165
- InterPro:   IPR018095
- InterPro:   IPR018094
- PANTHER:   PTHR10344
- TIGRFAMs:   TIGR00041

Pfam domain/function: PF02223 Thymidylate_kin

EC number: =2.7.4.9

Molecular weight: Translated: 23199; Mature: 23199

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFITFEGIDGSGKTTQSHLLAEYLSEIYGVNNVVLTREPGGTLLNESVRNLLFKAQGLDS
CEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHH
LSELLFFIAMRREHFVKIIKPSLMQKKIVICDRFIDSTIAYQGYGQGIDCSLIDQLNDLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHEE
IDVYPDITFIIDVDINESLSRSCKNGYEFADMEFYYRVRDGFYDIAKKNPHRCHVITDKS
EEECCCEEEEEEECCCHHHHHHHHCCCCCCCHHEEEEEHHCHHHHHCCCCCEEEEEECCC
ETYDIDDINFVHLEVIKVLQMV
CEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MFITFEGIDGSGKTTQSHLLAEYLSEIYGVNNVVLTREPGGTLLNESVRNLLFKAQGLDS
CEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHH
LSELLFFIAMRREHFVKIIKPSLMQKKIVICDRFIDSTIAYQGYGQGIDCSLIDQLNDLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHEE
IDVYPDITFIIDVDINESLSRSCKNGYEFADMEFYYRVRDGFYDIAKKNPHRCHVITDKS
EEECCCEEEEEEECCCHHHHHHHHCCCCCCCHHEEEEEHHCHHHHHCCCCCEEEEEECCC
ETYDIDDINFVHLEVIKVLQMV
CEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA