Click here to switch to the map view.

The map label for this gene is pheT [H]

Identifier: 88658312

GI number: 88658312

Start: 411211

End: 413577

Strand: Direct

Name: pheT [H]

Synonym: ECH_0434

Alternate gene names: 88658312

Gene position: 411211-413577 (Clockwise)

Preceding gene: 88657838

Following gene: 88657575

Centisome position: 34.96

GC content: 31.39

Gene sequence:

>2367_bases
ATGAGATTTACACTTTCGTGGTTAATGCAGTATCTTGATACAGATGTTTCTTTAGATATAATAGTTAATAAGCTTAGTGA
TATTGGTATAGAAGTAGATAACGTAGATTATTGTGAACACTTAAAAACTTTTGTAGTTGCTGAGGTATTGGAAGTAGTTC
CTCATCATGCAGCTAATAGGTTAAAGGTTTGTCAAGTATTTGATGGAAAGGAAACTTTTCAAGTTGTGTGTGGTGCTTCT
AATGTAAGAGCTGGGATAAAGTCTGTACTAGCACGTGTTGGCAGTATAATACCAAAAAATCAATCTGTGATTGAAGTAGT
AAAACTGCGAGGTGTTGAAAGTTATGGTATGCTATGTTCTAAGGATGAATTAGGAATAGTAGATCATAGTAATTCTAATG
ATGGGATAATTGAACTGTCATCCGCTTATAATGTAGGAAATACTTTCTTTTCTTGTGATCCAATTATAGAAGTTAGTATT
ACTCCAAATCGTGGGGATTGTTTAGGAGTATATGGGATAGCTCGTGATTTAGCTGCTGCTGGTATGGGGAAGTTGAAATG
CATAGATAATAATATTGTTAATGATTCTACTGCTTCTAATTCTTCTCCAGTTGATATTTGTATGCAAGCTGAAGGAATAG
TTAAGGGGAGATATATTAAGGGAATAAAAAATTGTGAAAGTCCTAAATGGTTAAAGGATAATCTGTTAGTATGTGGGATA
AATTCAGTTTCGTTTGTTGTTGATATAACAAACTATATAATGCTGTCTTTTAATAGACCACTTCATGTATATGATGCAAG
TAAGATAAAGAAGAAGTTAATTTTTAAGAAGGCAGATAGTCAAATGGAATTTTCTGCTCTTAATGATAAGAAGTATATTT
TAGGGACAGAAAACATTATAGCTGTTGACTCAAATGATAATATTCATAGTGTTGCTGGGATAATAGGTTCTCAGTTAAGT
AGTTGCTCATTGGAAACAGAAGATATTTTCTTAGAATCTGCATGGTTTAACCCTATGGATATTGTTTTATCTTCAAGAAA
AATTAAATTGTCTACTGATGCTAGTTATCGTTTTGAAAGGTTTGTTGACCCTAAGTTTTTACAGACTGGGCTAGATTTAG
CTACAAAAATGATATTGGAGTATTGTGGAGGGGAATTTTTTGATGTTGTTTCTGATGAAAATTATCTAATTAATGATATA
AGGTTAGATTTTTTGCCAGAATCTGTAAGAAAAGTGGGAAATGTTGATATTGCAACAAAACAAATTTTTGATTTTTTAGT
TAAGCTTGGTTTTTTTGTAGATAATAGTAATGAAGATGTGTGGAAAGTTACTGTTCCTTCATGGAGGTCTGATATTCAGC
ATTCTTCTGATATTGTTGAAGAAGTACTTAGATTATATGGGTATGATAAAATATGTGAAGAACCTATACCTATTTCTCAT
GTTGATGTAGTTGATAACTCTTATGATAGGTTGAGGTCAGTACTGTTGAGTGAAGGTTTGATGGAAGTTATTACTTGGTC
ATTTACAAGTATGACATTTGCTAAAAAGTTAGGATATGATTCAGAATTGTTGCTACTTGATAACCCTATTAATGACAAGT
TGAATTTAATGCGTCCTAGTGTTTTATTAAATTTACTACAGGTTGTTTCTGACAACCAAGCATATGGAAGTAGTGAAGTA
GCTATTTTTGAAATAGGTCAAATTTACAATATTGATAATGTATGTAGTGGCAATAGTTATGTAGTAGGTGGTGTTAGGTA
TGGGAATAATTTCCCGCGTAATCTTTATAAACTTGATAGATCTGTTGATATTTTTGATGTCAAATCTGATGTTATCAGCA
TATTACGGGAATTGAATATTAATTCCGATGCTGTAGAATTTAAAAAATCTGATAAACCATATCTTCACCCTGTAAAATCT
GCAGATGTGTATTTTAACAATATTTTGCTAGGGTATTTTGGGGAGTTACATCCTGATATTATACATTTGTACGAAATCAA
ACGTCCAGTAGTTTGCTTTGAAGTATTTTTATATAAAATTCCTCAAATCAATGTGATAAGTAAAGAGTTTGTTGAATCAT
GTTATCAGTTAGTAAAACGTGATTTTGCCTTTCTAATTGATAAAAATATAAGTGTACAGTGTTTAGTAGATGTTACTAAA
AATAGCAATAGTACTTTAATCGATAATGTTGTAGTGTTTGATTTATATGAAGGAGATAATATAGGTGATAATAAATTATC
TGTTGCATTGTCAGTTACTTTTAGGCCTGTTGACCACACATTAAATGATCAAGAGATAAAAGATGCATCTGATCGCATAA
TTAGTGCTGTTGCCCAGGAGCTAGGTGGAGTTCTTAGATCTTTTTAA

Upstream 100 bases:

>100_bases
TATAGAACTTGTTGATAGGCAGATAATAACTGTTGAGAGTAACAAGAATTCCTAGTTTTATTTTAAGTATTATTATGTTT
TTTATCTCAAGTGACTTATT

Downstream 100 bases:

>100_bases
GTGTGTAGTGTGCTATTCGTTTTATATATATTTTTTAGATGTTATTTAATCACTACAGTACATAAGTCAGTATGTAGTTT
TTATAATTGTGCATTTTACT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 788; Mature: 788

Protein sequence:

>788_residues
MRFTLSWLMQYLDTDVSLDIIVNKLSDIGIEVDNVDYCEHLKTFVVAEVLEVVPHHAANRLKVCQVFDGKETFQVVCGAS
NVRAGIKSVLARVGSIIPKNQSVIEVVKLRGVESYGMLCSKDELGIVDHSNSNDGIIELSSAYNVGNTFFSCDPIIEVSI
TPNRGDCLGVYGIARDLAAAGMGKLKCIDNNIVNDSTASNSSPVDICMQAEGIVKGRYIKGIKNCESPKWLKDNLLVCGI
NSVSFVVDITNYIMLSFNRPLHVYDASKIKKKLIFKKADSQMEFSALNDKKYILGTENIIAVDSNDNIHSVAGIIGSQLS
SCSLETEDIFLESAWFNPMDIVLSSRKIKLSTDASYRFERFVDPKFLQTGLDLATKMILEYCGGEFFDVVSDENYLINDI
RLDFLPESVRKVGNVDIATKQIFDFLVKLGFFVDNSNEDVWKVTVPSWRSDIQHSSDIVEEVLRLYGYDKICEEPIPISH
VDVVDNSYDRLRSVLLSEGLMEVITWSFTSMTFAKKLGYDSELLLLDNPINDKLNLMRPSVLLNLLQVVSDNQAYGSSEV
AIFEIGQIYNIDNVCSGNSYVVGGVRYGNNFPRNLYKLDRSVDIFDVKSDVISILRELNINSDAVEFKKSDKPYLHPVKS
ADVYFNNILLGYFGELHPDIIHLYEIKRPVVCFEVFLYKIPQINVISKEFVESCYQLVKRDFAFLIDKNISVQCLVDVTK
NSNSTLIDNVVVFDLYEGDNIGDNKLSVALSVTFRPVDHTLNDQEIKDASDRIISAVAQELGGVLRSF

Sequences:

>Translated_788_residues
MRFTLSWLMQYLDTDVSLDIIVNKLSDIGIEVDNVDYCEHLKTFVVAEVLEVVPHHAANRLKVCQVFDGKETFQVVCGAS
NVRAGIKSVLARVGSIIPKNQSVIEVVKLRGVESYGMLCSKDELGIVDHSNSNDGIIELSSAYNVGNTFFSCDPIIEVSI
TPNRGDCLGVYGIARDLAAAGMGKLKCIDNNIVNDSTASNSSPVDICMQAEGIVKGRYIKGIKNCESPKWLKDNLLVCGI
NSVSFVVDITNYIMLSFNRPLHVYDASKIKKKLIFKKADSQMEFSALNDKKYILGTENIIAVDSNDNIHSVAGIIGSQLS
SCSLETEDIFLESAWFNPMDIVLSSRKIKLSTDASYRFERFVDPKFLQTGLDLATKMILEYCGGEFFDVVSDENYLINDI
RLDFLPESVRKVGNVDIATKQIFDFLVKLGFFVDNSNEDVWKVTVPSWRSDIQHSSDIVEEVLRLYGYDKICEEPIPISH
VDVVDNSYDRLRSVLLSEGLMEVITWSFTSMTFAKKLGYDSELLLLDNPINDKLNLMRPSVLLNLLQVVSDNQAYGSSEV
AIFEIGQIYNIDNVCSGNSYVVGGVRYGNNFPRNLYKLDRSVDIFDVKSDVISILRELNINSDAVEFKKSDKPYLHPVKS
ADVYFNNILLGYFGELHPDIIHLYEIKRPVVCFEVFLYKIPQINVISKEFVESCYQLVKRDFAFLIDKNISVQCLVDVTK
NSNSTLIDNVVVFDLYEGDNIGDNKLSVALSVTFRPVDHTLNDQEIKDASDRIISAVAQELGGVLRSF
>Mature_788_residues
MRFTLSWLMQYLDTDVSLDIIVNKLSDIGIEVDNVDYCEHLKTFVVAEVLEVVPHHAANRLKVCQVFDGKETFQVVCGAS
NVRAGIKSVLARVGSIIPKNQSVIEVVKLRGVESYGMLCSKDELGIVDHSNSNDGIIELSSAYNVGNTFFSCDPIIEVSI
TPNRGDCLGVYGIARDLAAAGMGKLKCIDNNIVNDSTASNSSPVDICMQAEGIVKGRYIKGIKNCESPKWLKDNLLVCGI
NSVSFVVDITNYIMLSFNRPLHVYDASKIKKKLIFKKADSQMEFSALNDKKYILGTENIIAVDSNDNIHSVAGIIGSQLS
SCSLETEDIFLESAWFNPMDIVLSSRKIKLSTDASYRFERFVDPKFLQTGLDLATKMILEYCGGEFFDVVSDENYLINDI
RLDFLPESVRKVGNVDIATKQIFDFLVKLGFFVDNSNEDVWKVTVPSWRSDIQHSSDIVEEVLRLYGYDKICEEPIPISH
VDVVDNSYDRLRSVLLSEGLMEVITWSFTSMTFAKKLGYDSELLLLDNPINDKLNLMRPSVLLNLLQVVSDNQAYGSSEV
AIFEIGQIYNIDNVCSGNSYVVGGVRYGNNFPRNLYKLDRSVDIFDVKSDVISILRELNINSDAVEFKKSDKPYLHPVKS
ADVYFNNILLGYFGELHPDIIHLYEIKRPVVCFEVFLYKIPQINVISKEFVESCYQLVKRDFAFLIDKNISVQCLVDVTK
NSNSTLIDNVVVFDLYEGDNIGDNKLSVALSVTFRPVDHTLNDQEIKDASDRIISAVAQELGGVLRSF

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI124028525, Length=398, Percent_Identity=24.1206030150754, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788006, Length=809, Percent_Identity=31.0259579728059, Blast_Score=391, Evalue=1e-109,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 88308; Mature: 88308

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTLSWLMQYLDTDVSLDIIVNKLSDIGIEVDNVDYCEHLKTFVVAEVLEVVPHHAANR
CEEEHHHHHHHHCCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCHHCCCC
LKVCQVFDGKETFQVVCGASNVRAGIKSVLARVGSIIPKNQSVIEVVKLRGVESYGMLCS
EEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHCCEEC
KDELGIVDHSNSNDGIIELSSAYNVGNTFFSCDPIIEVSITPNRGDCLGVYGIARDLAAA
CCCCCEEECCCCCCCEEEEECCCCCCCCEEECCCEEEEEECCCCCCEEEHHHHHHHHHHC
GMGKLKCIDNNIVNDSTASNSSPVDICMQAEGIVKGRYIKGIKNCESPKWLKDNLLVCGI
CCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCHHCCEEEEEC
NSVSFVVDITNYIMLSFNRPLHVYDASKIKKKLIFKKADSQMEFSALNDKKYILGTENII
CCCEEEEEEECEEEEEECCCEEEECHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEE
AVDSNDNIHSVAGIIGSQLSSCSLETEDIFLESAWFNPMDIVLSSRKIKLSTDASYRFER
EECCCCCHHHHHHHHHHHHHCCCCCHHHEEEECCCCCHHHHEECCCEEEEECCCCHHHHH
FVDPKFLQTGLDLATKMILEYCGGEFFDVVSDENYLINDIRLDFLPESVRKVGNVDIATK
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEECCCHHHHHHCCCCHHHH
QIFDFLVKLGFFVDNSNEDVWKVTVPSWRSDIQHSSDIVEEVLRLYGYDKICEEPIPISH
HHHHHHHHHHHEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCE
VDVVDNSYDRLRSVLLSEGLMEVITWSFTSMTFAKKLGYDSELLLLDNPINDKLNLMRPS
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCHH
VLLNLLQVVSDNQAYGSSEVAIFEIGQIYNIDNVCSGNSYVVGGVRYGNNFPRNLYKLDR
HHHHHHHHHCCCCCCCCCCEEEEEECCEECCCCCCCCCCEEEEEEECCCCCCHHHHHHCC
SVDIFDVKSDVISILRELNINSDAVEFKKSDKPYLHPVKSADVYFNNILLGYFGELHPDI
CCEEEECHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
IHLYEIKRPVVCFEVFLYKIPQINVISKEFVESCYQLVKRDFAFLIDKNISVQCLVDVTK
EEHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEC
NSNSTLIDNVVVFDLYEGDNIGDNKLSVALSVTFRPVDHTLNDQEIKDASDRIISAVAQE
CCCCEEECCEEEEEEECCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LGGVLRSF
HHHHHHCC
>Mature Secondary Structure
MRFTLSWLMQYLDTDVSLDIIVNKLSDIGIEVDNVDYCEHLKTFVVAEVLEVVPHHAANR
CEEEHHHHHHHHCCCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCHHCCCC
LKVCQVFDGKETFQVVCGASNVRAGIKSVLARVGSIIPKNQSVIEVVKLRGVESYGMLCS
EEEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHCCEEC
KDELGIVDHSNSNDGIIELSSAYNVGNTFFSCDPIIEVSITPNRGDCLGVYGIARDLAAA
CCCCCEEECCCCCCCEEEEECCCCCCCCEEECCCEEEEEECCCCCCEEEHHHHHHHHHHC
GMGKLKCIDNNIVNDSTASNSSPVDICMQAEGIVKGRYIKGIKNCESPKWLKDNLLVCGI
CCCCEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCHHCCEEEEEC
NSVSFVVDITNYIMLSFNRPLHVYDASKIKKKLIFKKADSQMEFSALNDKKYILGTENII
CCCEEEEEEECEEEEEECCCEEEECHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEE
AVDSNDNIHSVAGIIGSQLSSCSLETEDIFLESAWFNPMDIVLSSRKIKLSTDASYRFER
EECCCCCHHHHHHHHHHHHHCCCCCHHHEEEECCCCCHHHHEECCCEEEEECCCCHHHHH
FVDPKFLQTGLDLATKMILEYCGGEFFDVVSDENYLINDIRLDFLPESVRKVGNVDIATK
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEECCCHHHHHHCCCCHHHH
QIFDFLVKLGFFVDNSNEDVWKVTVPSWRSDIQHSSDIVEEVLRLYGYDKICEEPIPISH
HHHHHHHHHHHEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCE
VDVVDNSYDRLRSVLLSEGLMEVITWSFTSMTFAKKLGYDSELLLLDNPINDKLNLMRPS
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCHH
VLLNLLQVVSDNQAYGSSEVAIFEIGQIYNIDNVCSGNSYVVGGVRYGNNFPRNLYKLDR
HHHHHHHHHCCCCCCCCCCEEEEEECCEECCCCCCCCCCEEEEEEECCCCCCHHHHHHCC
SVDIFDVKSDVISILRELNINSDAVEFKKSDKPYLHPVKSADVYFNNILLGYFGELHPDI
CCEEEECHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
IHLYEIKRPVVCFEVFLYKIPQINVISKEFVESCYQLVKRDFAFLIDKNISVQCLVDVTK
EEHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEC
NSNSTLIDNVVVFDLYEGDNIGDNKLSVALSVTFRPVDHTLNDQEIKDASDRIISAVAQE
CCCCEEECCEEEEEEECCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LGGVLRSF
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA