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The map label for this gene is pccA [H]

Identifier: 88658256

GI number: 88658256

Start: 468541

End: 470508

Strand: Direct

Name: pccA [H]

Synonym: ECH_0487

Alternate gene names: 88658256

Gene position: 468541-470508 (Clockwise)

Preceding gene: 88657876

Following gene: 88657582

Centisome position: 39.83

GC content: 31.86

Gene sequence:

>1968_bases
ATGATAAAAAAAATATTAATAGCAAATAGAGGTGAAATTGCATGTAGAGTAATGAGAACTGCTCGTAAAATGGGAATATC
TTGTGTAGCAGTATATTCAAATGCAGATGTGTACTCATTACATGTTTTATCTGCTGAAGAAGCAGTAAATATTGGTCCTG
CTCCAGTAAATCAGAGTTATTTGAATATGGAGAAAATTTGTGAGGTAGCTTGTAATACAGGTGTAGACGCTGTGCATCCT
GGATATGGTTTTTTATCAGAAAATGCTGATTTTCCTGAAAAACTTGAGCAGTATAATATTAAGTTTATTGGTCCAAGTTC
TACCTCAATCAGAATGATGGCTGATAAAATTACTTCTAAAAAAATAGCCGAGTCTGCAAAGGTGAATATTATTCCTGGAT
ATATGGGAATTGTTGATTCTGTTCATGAAGCAAAAGAAATAGCTAAATCTATAGGGTTTCCAGTAATGATAAAAGCTACT
GCTGGTGGTGGTGGTAAAGGTATGAGAATTGTGAAGTCAAGTGAAGAAATAGAGCAAGCTTTTACTTCTGCAACAAATGA
AGCAGCAAAAAATTTTAGGGACGGTAGAATTTTTATAGAAAAATACGTTGAGTTACCAAGACATATTGAAATACAAATTA
TAGCTGATAAGCATGGCAATATTGTGTGTTTGGGTGAAAGAGAATGTTCTATACAACGACATAATCAAAAAGTTATTGAG
GAAACTCCTAGTCCGTTTCTTGACGAAGAAACGAGGCAAAAAATGTATCAGCAATGTGTGAATCTTGCGAAAAAAGTAGG
GTATTATTCTGCTGGTACTATTGAATTTATAGTTGATCAAGATAAGCAATTTTATTTCTTAGAAATGAATACTAGATTAC
AAGTTGAACACCCTGTTACAGAGCTTGTGACTGGAATTGATATTGTCGAAGAAATGATTCGAATTGCTGATGGTGAAGAA
TTGAGATTTACTCAACAAGATGTGAAATTTACTGGTTCGGCAATAGAAGCAAGAGTTTATGCAGAAAATCCTACAAAGAA
TTTTTTACCTTCTAGTGGAAGAATTGCTTATTATTCTGCTCCTATGCCTAATGATAATTTACGGATTGATAGTGGGGTAT
TTGAGGGAGCAGAAGTCAGCATGTTTTATGATCCTATGATTGCTAAAGTTTGTACTTATGGTAAAAATAGAGATGAAGCA
GTGAGTTTTATGCAAAGATATTTAAATGAGTTTTATATTGGTGGCATTGCTAATAATATAGACTTTTTGTTATCTGTATT
TCATCATCCTGTATTTATATCTGGAAATATTAATACGAAGTTTATTGAGCAATTTTATTTTGATGGGTTTCAAGGAAATC
CTCTAACCAAAGCTTGTATTAAACTTTTTATTTTGACCTCACTATGTATATTCTTTCAAGATGAATATGGTATTCATGGG
GTTGAATTATGTGAAAATAGAGAATTAGCTGTTTATGTTGATGGTCAAAAATATTTGATTAGTGCTAAATATGAAAATGG
TAGAGTTTTGGCAATTTATGACCAGTGTGAGTATTTAGTTGTAAGTACATGGAATGTGAATTTTAAAATATTACAGATCC
AAGTAAATAATGATGAGGTATTTCATGTAAAGGTTGATAGTAGATTAAATAAATATCAACTTAAATATTCTGCTATGAGT
GCATTGTGTGCAGTATATAAACCTTGTGTATCTGATCTACTACCTATAATGCCTCAGATTAGTGGGGAAGAGTTATATTC
TAGTAATGTATGTTCTCCTATTTCTGGTATGATAGTTAAAATTTATGTTAAACAAGGAGAAGAAGTTCAACCAGGTCAAC
CTTTGTTAGTAATTGAAGCTATGAAGATGGAAAATGTGATATATTCAGATGTAAAGTCAATTGTGAAATCTGTATTATTT
TCAGAAGGAAACAGTGTAGCTACTGGTGATGTTATTATAGAGTTTTAG

Upstream 100 bases:

>100_bases
GTGAAATATTACTTGTGGTATGGTTGTATATAAGTAAAATTTTAGACTATCTTTATATATAAATGAAGGAATTACCTTTT
AATTAAGAGTATTTAAGATA

Downstream 100 bases:

>100_bases
TTGGTTATATTTATTTTTTATTGTATAAAGACTGCTTACACCTTAATAGTATAGATTTTAATAAATTAATAGATATAAAT
TGTGATTGTGCTAAAATACG

Product: propionyl-CoA carboxylase, alpha subunit

Products: NA

Alternate protein names: Biotin carboxylase; Biotin carboxyl carrier protein; BCCP [H]

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MIKKILIANRGEIACRVMRTARKMGISCVAVYSNADVYSLHVLSAEEAVNIGPAPVNQSYLNMEKICEVACNTGVDAVHP
GYGFLSENADFPEKLEQYNIKFIGPSSTSIRMMADKITSKKIAESAKVNIIPGYMGIVDSVHEAKEIAKSIGFPVMIKAT
AGGGGKGMRIVKSSEEIEQAFTSATNEAAKNFRDGRIFIEKYVELPRHIEIQIIADKHGNIVCLGERECSIQRHNQKVIE
ETPSPFLDEETRQKMYQQCVNLAKKVGYYSAGTIEFIVDQDKQFYFLEMNTRLQVEHPVTELVTGIDIVEEMIRIADGEE
LRFTQQDVKFTGSAIEARVYAENPTKNFLPSSGRIAYYSAPMPNDNLRIDSGVFEGAEVSMFYDPMIAKVCTYGKNRDEA
VSFMQRYLNEFYIGGIANNIDFLLSVFHHPVFISGNINTKFIEQFYFDGFQGNPLTKACIKLFILTSLCIFFQDEYGIHG
VELCENRELAVYVDGQKYLISAKYENGRVLAIYDQCEYLVVSTWNVNFKILQIQVNNDEVFHVKVDSRLNKYQLKYSAMS
ALCAVYKPCVSDLLPIMPQISGEELYSSNVCSPISGMIVKIYVKQGEEVQPGQPLLVIEAMKMENVIYSDVKSIVKSVLF
SEGNSVATGDVIIEF

Sequences:

>Translated_655_residues
MIKKILIANRGEIACRVMRTARKMGISCVAVYSNADVYSLHVLSAEEAVNIGPAPVNQSYLNMEKICEVACNTGVDAVHP
GYGFLSENADFPEKLEQYNIKFIGPSSTSIRMMADKITSKKIAESAKVNIIPGYMGIVDSVHEAKEIAKSIGFPVMIKAT
AGGGGKGMRIVKSSEEIEQAFTSATNEAAKNFRDGRIFIEKYVELPRHIEIQIIADKHGNIVCLGERECSIQRHNQKVIE
ETPSPFLDEETRQKMYQQCVNLAKKVGYYSAGTIEFIVDQDKQFYFLEMNTRLQVEHPVTELVTGIDIVEEMIRIADGEE
LRFTQQDVKFTGSAIEARVYAENPTKNFLPSSGRIAYYSAPMPNDNLRIDSGVFEGAEVSMFYDPMIAKVCTYGKNRDEA
VSFMQRYLNEFYIGGIANNIDFLLSVFHHPVFISGNINTKFIEQFYFDGFQGNPLTKACIKLFILTSLCIFFQDEYGIHG
VELCENRELAVYVDGQKYLISAKYENGRVLAIYDQCEYLVVSTWNVNFKILQIQVNNDEVFHVKVDSRLNKYQLKYSAMS
ALCAVYKPCVSDLLPIMPQISGEELYSSNVCSPISGMIVKIYVKQGEEVQPGQPLLVIEAMKMENVIYSDVKSIVKSVLF
SEGNSVATGDVIIEF
>Mature_655_residues
MIKKILIANRGEIACRVMRTARKMGISCVAVYSNADVYSLHVLSAEEAVNIGPAPVNQSYLNMEKICEVACNTGVDAVHP
GYGFLSENADFPEKLEQYNIKFIGPSSTSIRMMADKITSKKIAESAKVNIIPGYMGIVDSVHEAKEIAKSIGFPVMIKAT
AGGGGKGMRIVKSSEEIEQAFTSATNEAAKNFRDGRIFIEKYVELPRHIEIQIIADKHGNIVCLGERECSIQRHNQKVIE
ETPSPFLDEETRQKMYQQCVNLAKKVGYYSAGTIEFIVDQDKQFYFLEMNTRLQVEHPVTELVTGIDIVEEMIRIADGEE
LRFTQQDVKFTGSAIEARVYAENPTKNFLPSSGRIAYYSAPMPNDNLRIDSGVFEGAEVSMFYDPMIAKVCTYGKNRDEA
VSFMQRYLNEFYIGGIANNIDFLLSVFHHPVFISGNINTKFIEQFYFDGFQGNPLTKACIKLFILTSLCIFFQDEYGIHG
VELCENRELAVYVDGQKYLISAKYENGRVLAIYDQCEYLVVSTWNVNFKILQIQVNNDEVFHVKVDSRLNKYQLKYSAMS
ALCAVYKPCVSDLLPIMPQISGEELYSSNVCSPISGMIVKIYVKQGEEVQPGQPLLVIEAMKMENVIYSDVKSIVKSVLF
SEGNSVATGDVIIEF

Specific function: This protein carries two functions:biotin carboxyl carrier protein and biotin carboxyltransferase [H]

COG id: COG4770

COG function: function code I; Acetyl/propionyl-CoA carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotinyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI189095269, Length=678, Percent_Identity=45.8702064896755, Blast_Score=563, Evalue=1e-160,
Organism=Homo sapiens, GI65506442, Length=678, Percent_Identity=45.8702064896755, Blast_Score=562, Evalue=1e-160,
Organism=Homo sapiens, GI295821183, Length=657, Percent_Identity=45.9665144596651, Blast_Score=538, Evalue=1e-153,
Organism=Homo sapiens, GI116805327, Length=445, Percent_Identity=51.685393258427, Blast_Score=481, Evalue=1e-135,
Organism=Homo sapiens, GI106049528, Length=449, Percent_Identity=44.7661469933185, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI106049295, Length=449, Percent_Identity=44.7661469933185, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI106049292, Length=449, Percent_Identity=44.7661469933185, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI38679960, Length=513, Percent_Identity=31.1890838206628, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI38679974, Length=513, Percent_Identity=31.1890838206628, Blast_Score=247, Evalue=3e-65,
Organism=Homo sapiens, GI38679977, Length=513, Percent_Identity=31.1890838206628, Blast_Score=247, Evalue=3e-65,
Organism=Homo sapiens, GI38679967, Length=513, Percent_Identity=31.1890838206628, Blast_Score=247, Evalue=3e-65,
Organism=Homo sapiens, GI38679971, Length=513, Percent_Identity=31.1890838206628, Blast_Score=247, Evalue=3e-65,
Organism=Homo sapiens, GI134142062, Length=511, Percent_Identity=31.7025440313112, Blast_Score=245, Evalue=1e-64,
Organism=Escherichia coli, GI1789654, Length=445, Percent_Identity=44.9438202247191, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1786216, Length=326, Percent_Identity=22.3926380368098, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17567343, Length=680, Percent_Identity=42.5, Blast_Score=516, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71987519, Length=668, Percent_Identity=40.5688622754491, Blast_Score=464, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17562816, Length=465, Percent_Identity=43.6559139784946, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71997168, Length=510, Percent_Identity=32.156862745098, Blast_Score=237, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI71997163, Length=510, Percent_Identity=32.156862745098, Blast_Score=237, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI133931226, Length=733, Percent_Identity=27.8308321964529, Blast_Score=229, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6319685, Length=449, Percent_Identity=44.3207126948775, Blast_Score=401, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6319695, Length=448, Percent_Identity=42.8571428571429, Blast_Score=350, Evalue=4e-97,
Organism=Saccharomyces cerevisiae, GI6321376, Length=448, Percent_Identity=42.8571428571429, Blast_Score=334, Evalue=3e-92,
Organism=Saccharomyces cerevisiae, GI6323863, Length=731, Percent_Identity=29.1381668946648, Blast_Score=256, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6324343, Length=519, Percent_Identity=32.3699421965318, Blast_Score=242, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24651757, Length=681, Percent_Identity=40.5286343612335, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24651759, Length=643, Percent_Identity=38.8802488335925, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24652212, Length=448, Percent_Identity=44.1964285714286, Blast_Score=364, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652210, Length=448, Percent_Identity=44.1964285714286, Blast_Score=364, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652214, Length=448, Percent_Identity=44.1964285714286, Blast_Score=364, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19921944, Length=448, Percent_Identity=44.1964285714286, Blast_Score=364, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652216, Length=448, Percent_Identity=44.1964285714286, Blast_Score=364, Evalue=1e-100,
Organism=Drosophila melanogaster, GI281363050, Length=448, Percent_Identity=44.1964285714286, Blast_Score=364, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652224, Length=448, Percent_Identity=44.1964285714286, Blast_Score=364, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652222, Length=448, Percent_Identity=44.1964285714286, Blast_Score=364, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652220, Length=448, Percent_Identity=44.1964285714286, Blast_Score=364, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652218, Length=448, Percent_Identity=44.1964285714286, Blast_Score=364, Evalue=1e-100,
Organism=Drosophila melanogaster, GI161076409, Length=513, Percent_Identity=30.2144249512671, Blast_Score=238, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24586458, Length=505, Percent_Identity=30.4950495049505, Blast_Score=238, Evalue=1e-62,
Organism=Drosophila melanogaster, GI161076407, Length=505, Percent_Identity=30.4950495049505, Blast_Score=238, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24586460, Length=505, Percent_Identity=30.4950495049505, Blast_Score=238, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR001882
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR000089
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054
- InterPro:   IPR011053 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00364 Biotin_lipoyl; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.3.4.14 [H]

Molecular weight: Translated: 73483; Mature: 73483

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKILIANRGEIACRVMRTARKMGISCVAVYSNADVYSLHVLSAEEAVNIGPAPVNQSY
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEEHHHHCCCCCCCCCHHH
LNMEKICEVACNTGVDAVHPGYGFLSENADFPEKLEQYNIKFIGPSSTSIRMMADKITSK
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCEEEHHHHHHHHH
KIAESAKVNIIPGYMGIVDSVHEAKEIAKSIGFPVMIKATAGGGGKGMRIVKSSEEIEQA
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECHHHHHHH
FTSATNEAAKNFRDGRIFIEKYVELPRHIEIQIIADKHGNIVCLGERECSIQRHNQKVIE
HHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEECCCCEEEEECCCCCHHHHCHHHHH
ETPSPFLDEETRQKMYQQCVNLAKKVGYYSAGTIEFIVDQDKQFYFLEMNTRLQVEHPVT
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEEECCEEEECCCHH
ELVTGIDIVEEMIRIADGEELRFTQQDVKFTGSAIEARVYAENPTKNFLPSSGRIAYYSA
HHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCEEEEEEEECCCCCCCCCCCCCEEEEEC
PMPNDNLRIDSGVFEGAEVSMFYDPMIAKVCTYGKNRDEAVSFMQRYLNEFYIGGIANNI
CCCCCCEEEECCCCCCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCH
DFLLSVFHHPVFISGNINTKFIEQFYFDGFQGNPLTKACIKLFILTSLCIFFQDEYGIHG
HHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCH
VELCENRELAVYVDGQKYLISAKYENGRVLAIYDQCEYLVVSTWNVNFKILQIQVNNDEV
HHEECCCCEEEEECCCEEEEEEEECCCCEEEEECCCCEEEEEEECCEEEEEEEEECCCEE
FHVKVDSRLNKYQLKYSAMSALCAVYKPCVSDLLPIMPQISGEELYSSNVCSPISGMIVK
EEEEECCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCEEEE
IYVKQGEEVQPGQPLLVIEAMKMENVIYSDVKSIVKSVLFSEGNSVATGDVIIEF
EEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEC
>Mature Secondary Structure
MIKKILIANRGEIACRVMRTARKMGISCVAVYSNADVYSLHVLSAEEAVNIGPAPVNQSY
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEEHHHHCCCCCCCCCHHH
LNMEKICEVACNTGVDAVHPGYGFLSENADFPEKLEQYNIKFIGPSSTSIRMMADKITSK
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCEEEHHHHHHHHH
KIAESAKVNIIPGYMGIVDSVHEAKEIAKSIGFPVMIKATAGGGGKGMRIVKSSEEIEQA
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECHHHHHHH
FTSATNEAAKNFRDGRIFIEKYVELPRHIEIQIIADKHGNIVCLGERECSIQRHNQKVIE
HHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEECCCCEEEEECCCCCHHHHCHHHHH
ETPSPFLDEETRQKMYQQCVNLAKKVGYYSAGTIEFIVDQDKQFYFLEMNTRLQVEHPVT
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEEECCEEEECCCHH
ELVTGIDIVEEMIRIADGEELRFTQQDVKFTGSAIEARVYAENPTKNFLPSSGRIAYYSA
HHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCEEEEEEEECCCCCCCCCCCCCEEEEEC
PMPNDNLRIDSGVFEGAEVSMFYDPMIAKVCTYGKNRDEAVSFMQRYLNEFYIGGIANNI
CCCCCCEEEECCCCCCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCH
DFLLSVFHHPVFISGNINTKFIEQFYFDGFQGNPLTKACIKLFILTSLCIFFQDEYGIHG
HHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCH
VELCENRELAVYVDGQKYLISAKYENGRVLAIYDQCEYLVVSTWNVNFKILQIQVNNDEV
HHEECCCCEEEEECCCEEEEEEEECCCCEEEEECCCCEEEEEEECCEEEEEEEEECCCEE
FHVKVDSRLNKYQLKYSAMSALCAVYKPCVSDLLPIMPQISGEELYSSNVCSPISGMIVK
EEEEECCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCEEEE
IYVKQGEEVQPGQPLLVIEAMKMENVIYSDVKSIVKSVLFSEGNSVATGDVIIEF
EEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]