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The map label for this gene is bcr [H]

Identifier: 88658142

GI number: 88658142

Start: 306388

End: 307596

Strand: Direct

Name: bcr [H]

Synonym: ECH_0318

Alternate gene names: 88658142

Gene position: 306388-307596 (Clockwise)

Preceding gene: 88658661

Following gene: 88658483

Centisome position: 26.05

GC content: 30.11

Gene sequence:

>1209_bases
ATGAAAATTTTAAAAAGAAATATTTTAATTCTTATTATTGCCGTTATTGTTTTATCTGATATATCTTCAGATATATATTT
ACCTGGACTACCGAAAATTAGTGATTTTTTTTCTGTAGATTATGCTATCGCACAGCTAACAATTAGCTTGAATTTAGCTG
GAATATCTATTTCAGGGTTATTATATGGTCCACTGTCTGATTATTATGGACGTAGACCTATTATGTTGTTAGGTGTGGGA
ATATTTACATTAGCAAGTGTAGCATGTTGTTTTGCTAGCAATATTACTGTGTTAATTATTATGCGTTTTATTCAGGGATT
TGGTGCAGGTGTAGCTGGAGTTGTTGGCTATGCTATTATCAAAGATATGTACTCTGGAAGTGACTGTGCTAAAAATATTT
CTATAGTAAATATGGCAGTAGCATTTTCTCCAGTATTGGGACCGATTTTAGGTAGTGCCATTATAGCACATGGTTATCAT
TGGAATATGCTGTTTATAGCAATATCAATAATGGCAATAATAGTTCTAATATGTCTATTTGTGTTTTTGAAAGAGACTAT
TGTTCTTGAGAAAATTAATAGTAATATCTCTTGCTTGTCAATTATGCGGAAGTATAAAGAGCTTGTTTTAAATATTCGAT
TTTGTGGTTTTGCTTTAATTCAAAGTTTTACTATTATGTGGATTTGGTCATGTATAGCTAATTTACCATTTATTTTTGTA
AAAGATATGGGTGTACCTGTTGGTTATTATGGCTATTTTGTGGCTATTAATGTAGCTGCATATATTGTTGGTGCAATGCT
AAATCAAAGATTTGTTGAGAGATTTGGAATAAATAATATGTTACTTTTAGGATTAATTTTAACTACTTTATCAGATGTAA
GTGTCCTAATACTCTATCAAATAGTTGAAATTACTCCTTTATTGGCAGAAATTATGTGGCTTCCATCTGGTATGGGTATT
GCTTTTATTCTTGGAAATAATATGACTGCTGCGTTTTCTGAAATTAGGGAGCCAGGTATAGGTTCTGCTTTTATTCTGTT
TTTGCAAACAATTTTTGGTGCACTTGGTATTTATATTTTAGGATGTTTTTATGATGGTACTCTGATTCCAGTGATACTAT
TTCCAATAATTTGTTCTACAATTTGCATTGTGATATATATTCTTTTAAGGAAAGATAAAAATAATAAATCTGAATTCATT
AGTGCATGA

Upstream 100 bases:

>100_bases
TTGGTTTTAAAGATTTAGTTCTATTTAATTATCTACGATAATTTTATATGAGATGTTATATTAGCAATGTGTTTATTAAT
TAAGGATTCCATTTTAGTGT

Downstream 100 bases:

>100_bases
TTATTGTACTGTTTAACAATAGGAAGTAAAATTGTGATTCTAAGTGTTTTTTATTTATAAAATAGGAAAAAATGGTAGAT
TTGTGTGAATATATGTTTAG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKILKRNILILIIAVIVLSDISSDIYLPGLPKISDFFSVDYAIAQLTISLNLAGISISGLLYGPLSDYYGRRPIMLLGVG
IFTLASVACCFASNITVLIIMRFIQGFGAGVAGVVGYAIIKDMYSGSDCAKNISIVNMAVAFSPVLGPILGSAIIAHGYH
WNMLFIAISIMAIIVLICLFVFLKETIVLEKINSNISCLSIMRKYKELVLNIRFCGFALIQSFTIMWIWSCIANLPFIFV
KDMGVPVGYYGYFVAINVAAYIVGAMLNQRFVERFGINNMLLLGLILTTLSDVSVLILYQIVEITPLLAEIMWLPSGMGI
AFILGNNMTAAFSEIREPGIGSAFILFLQTIFGALGIYILGCFYDGTLIPVILFPIICSTICIVIYILLRKDKNNKSEFI
SA

Sequences:

>Translated_402_residues
MKILKRNILILIIAVIVLSDISSDIYLPGLPKISDFFSVDYAIAQLTISLNLAGISISGLLYGPLSDYYGRRPIMLLGVG
IFTLASVACCFASNITVLIIMRFIQGFGAGVAGVVGYAIIKDMYSGSDCAKNISIVNMAVAFSPVLGPILGSAIIAHGYH
WNMLFIAISIMAIIVLICLFVFLKETIVLEKINSNISCLSIMRKYKELVLNIRFCGFALIQSFTIMWIWSCIANLPFIFV
KDMGVPVGYYGYFVAINVAAYIVGAMLNQRFVERFGINNMLLLGLILTTLSDVSVLILYQIVEITPLLAEIMWLPSGMGI
AFILGNNMTAAFSEIREPGIGSAFILFLQTIFGALGIYILGCFYDGTLIPVILFPIICSTICIVIYILLRKDKNNKSEFI
SA
>Mature_402_residues
MKILKRNILILIIAVIVLSDISSDIYLPGLPKISDFFSVDYAIAQLTISLNLAGISISGLLYGPLSDYYGRRPIMLLGVG
IFTLASVACCFASNITVLIIMRFIQGFGAGVAGVVGYAIIKDMYSGSDCAKNISIVNMAVAFSPVLGPILGSAIIAHGYH
WNMLFIAISIMAIIVLICLFVFLKETIVLEKINSNISCLSIMRKYKELVLNIRFCGFALIQSFTIMWIWSCIANLPFIFV
KDMGVPVGYYGYFVAINVAAYIVGAMLNQRFVERFGINNMLLLGLILTTLSDVSVLILYQIVEITPLLAEIMWLPSGMGI
AFILGNNMTAAFSEIREPGIGSAFILFLQTIFGALGIYILGCFYDGTLIPVILFPIICSTICIVIYILLRKDKNNKSEFI
SA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=269, Percent_Identity=31.2267657992565, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790146, Length=251, Percent_Identity=31.4741035856574, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1790794, Length=253, Percent_Identity=29.6442687747036, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI48994889, Length=286, Percent_Identity=26.2237762237762, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787065, Length=264, Percent_Identity=29.1666666666667, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI87082312, Length=195, Percent_Identity=27.6923076923077, Blast_Score=78, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323000, Length=164, Percent_Identity=30.4878048780488, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319480, Length=187, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319657, Length=168, Percent_Identity=26.7857142857143, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321838, Length=163, Percent_Identity=28.8343558282209, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44065; Mature: 44065

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILKRNILILIIAVIVLSDISSDIYLPGLPKISDFFSVDYAIAQLTISLNLAGISISGL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEEEEEEE
LYGPLSDYYGRRPIMLLGVGIFTLASVACCFASNITVLIIMRFIQGFGAGVAGVVGYAII
EECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHH
KDMYSGSDCAKNISIVNMAVAFSPVLGPILGSAIIAHGYHWNMLFIAISIMAIIVLICLF
HHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VFLKETIVLEKINSNISCLSIMRKYKELVLNIRFCGFALIQSFTIMWIWSCIANLPFIFV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
KDMGVPVGYYGYFVAINVAAYIVGAMLNQRFVERFGINNMLLLGLILTTLSDVSVLILYQ
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IVEITPLLAEIMWLPSGMGIAFILGNNMTAAFSEIREPGIGSAFILFLQTIFGALGIYIL
HHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
GCFYDGTLIPVILFPIICSTICIVIYILLRKDKNNKSEFISA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
>Mature Secondary Structure
MKILKRNILILIIAVIVLSDISSDIYLPGLPKISDFFSVDYAIAQLTISLNLAGISISGL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEEEEEEEEEE
LYGPLSDYYGRRPIMLLGVGIFTLASVACCFASNITVLIIMRFIQGFGAGVAGVVGYAII
EECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHH
KDMYSGSDCAKNISIVNMAVAFSPVLGPILGSAIIAHGYHWNMLFIAISIMAIIVLICLF
HHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VFLKETIVLEKINSNISCLSIMRKYKELVLNIRFCGFALIQSFTIMWIWSCIANLPFIFV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
KDMGVPVGYYGYFVAINVAAYIVGAMLNQRFVERFGINNMLLLGLILTTLSDVSVLILYQ
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
IVEITPLLAEIMWLPSGMGIAFILGNNMTAAFSEIREPGIGSAFILFLQTIFGALGIYIL
HHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
GCFYDGTLIPVILFPIICSTICIVIYILLRKDKNNKSEFISA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]