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The map label for this gene is gmk

Identifier: 88658483

GI number: 88658483

Start: 311091

End: 311720

Strand: Direct

Name: gmk

Synonym: ECH_0322

Alternate gene names: 88658483

Gene position: 311091-311720 (Clockwise)

Preceding gene: 88658142

Following gene: 88657832

Centisome position: 26.45

GC content: 30.79

Gene sequence:

>630_bases
GTGAATGATAGTTTAAAAAGTAGGGGAATAATGTTAGTTATATCATCTCCTTCTGGAGGTGGTAAAACTACAATATCTCA
TTTGTTAATTAATGAATTACAAAATGATCTTGTTAGGTCAATTTCTGTTACTACTCGTGAACCTAGAGATGGTGAAATCA
ATGGTAAAGATTATTTTTTTGTAACTGAACCTGAGTTTATCAATTTGTGTAATACAAATCAGATGCTAGAGTATGCAAAA
GTTTTTGGGAATTACTATGGTATACCCCGTAAGTTTGTTACCGATAATATTGCTAATGGTGTTAGTGTATTGTTTAGTAT
TGATTGGCAAGGGGCGTTTAAATTAATTGATATTATGCGAGAACATGTGGTGAGTATATTCATATTACCTCCATCTATGG
AAGAGTTGCAAAGGCGACTATATAATCGTAGTGGAGAAAGCGATGTAATAAATAAAAGATTAGGAGAAGCTGCTTTTGAA
ATTAGTCATTGTTATCGTTATGACTATGTTATTGTTAACCATGATATTGAACAAAGTGTTTATCAGATTAAATGTATTTT
TACTTCAGAAAAATTGAAAACGCAACGTAGGGTATCTCTAAAACAATTTATTGATAATTGTATCAGATGA

Upstream 100 bases:

>100_bases
AAGTGTGGAAAGTGACATTGTTTAATATTTATATAATGGAGTTTTATTAAGTTGAGTTATATACAATATTATAATTATTA
TTTTTTTTAGGAAATGTAAG

Downstream 100 bases:

>100_bases
TTGTAACTCATCATAAATTTATAACCTAACGTTATTATTTATTTTATTAAGTAGATGATAAAATAAAAAATGTTATTTAC
TAAATCCTAATTATTTGTTC

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MNDSLKSRGIMLVISSPSGGGKTTISHLLINELQNDLVRSISVTTREPRDGEINGKDYFFVTEPEFINLCNTNQMLEYAK
VFGNYYGIPRKFVTDNIANGVSVLFSIDWQGAFKLIDIMREHVVSIFILPPSMEELQRRLYNRSGESDVINKRLGEAAFE
ISHCYRYDYVIVNHDIEQSVYQIKCIFTSEKLKTQRRVSLKQFIDNCIR

Sequences:

>Translated_209_residues
MNDSLKSRGIMLVISSPSGGGKTTISHLLINELQNDLVRSISVTTREPRDGEINGKDYFFVTEPEFINLCNTNQMLEYAK
VFGNYYGIPRKFVTDNIANGVSVLFSIDWQGAFKLIDIMREHVVSIFILPPSMEELQRRLYNRSGESDVINKRLGEAAFE
ISHCYRYDYVIVNHDIEQSVYQIKCIFTSEKLKTQRRVSLKQFIDNCIR
>Mature_209_residues
MNDSLKSRGIMLVISSPSGGGKTTISHLLINELQNDLVRSISVTTREPRDGEINGKDYFFVTEPEFINLCNTNQMLEYAK
VFGNYYGIPRKFVTDNIANGVSVLFSIDWQGAFKLIDIMREHVVSIFILPPSMEELQRRLYNRSGESDVINKRLGEAAFE
ISHCYRYDYVIVNHDIEQSVYQIKCIFTSEKLKTQRRVSLKQFIDNCIR

Specific function: Essential for recycling GMP and indirectly, cGMP

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain

Homologues:

Organism=Homo sapiens, GI226874831, Length=191, Percent_Identity=32.9842931937173, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI226874833, Length=191, Percent_Identity=32.9842931937173, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4504221, Length=191, Percent_Identity=32.9842931937173, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1790080, Length=204, Percent_Identity=43.6274509803922, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI193203132, Length=193, Percent_Identity=32.6424870466321, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17508835, Length=193, Percent_Identity=32.6424870466321, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71988041, Length=183, Percent_Identity=26.775956284153, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988048, Length=183, Percent_Identity=26.775956284153, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320662, Length=183, Percent_Identity=38.7978142076503, Blast_Score=124, Evalue=9e-30,
Organism=Drosophila melanogaster, GI21356141, Length=192, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KGUA_EHRCR (Q2GHE0)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507143.1
- ProteinModelPortal:   Q2GHE0
- SMR:   Q2GHE0
- STRING:   Q2GHE0
- GeneID:   3928029
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0322
- TIGR:   ECH_0322
- eggNOG:   COG0194
- HOGENOM:   HBG671982
- OMA:   DIDWQGT
- PhylomeDB:   Q2GHE0
- ProtClustDB:   PRK00300
- BioCyc:   ECHA205920:ECH_0322-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00328
- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665
- SMART:   SM00072
- TIGRFAMs:   TIGR03263

Pfam domain/function: PF00625 Guanylate_kin

EC number: =2.7.4.8

Molecular weight: Translated: 24089; Mature: 24089

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00856 GUANYLATE_KINASE_1; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSLKSRGIMLVISSPSGGGKTTISHLLINELQNDLVRSISVTTREPRDGEINGKDYFF
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
VTEPEFINLCNTNQMLEYAKVFGNYYGIPRKFVTDNIANGVSVLFSIDWQGAFKLIDIMR
EECCHHEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHH
EHVVSIFILPPSMEELQRRLYNRSGESDVINKRLGEAAFEISHCYRYDYVIVNHDIEQSV
HHHEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCHHHCE
YQIKCIFTSEKLKTQRRVSLKQFIDNCIR
EEEEEEEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNDSLKSRGIMLVISSPSGGGKTTISHLLINELQNDLVRSISVTTREPRDGEINGKDYFF
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
VTEPEFINLCNTNQMLEYAKVFGNYYGIPRKFVTDNIANGVSVLFSIDWQGAFKLIDIMR
EECCHHEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHH
EHVVSIFILPPSMEELQRRLYNRSGESDVINKRLGEAAFEISHCYRYDYVIVNHDIEQSV
HHHEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCHHHCE
YQIKCIFTSEKLKTQRRVSLKQFIDNCIR
EEEEEEEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA