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The map label for this gene is nrdF [H]

Identifier: 88658048

GI number: 88658048

Start: 773381

End: 774355

Strand: Reverse

Name: nrdF [H]

Synonym: ECH_0766

Alternate gene names: 88658048

Gene position: 774355-773381 (Counterclockwise)

Preceding gene: 88658290

Following gene: 88657760

Centisome position: 65.83

GC content: 30.67

Gene sequence:

>975_bases
ATGTCATTATTAGATTCTAGGCCAATTTATAAGCCATTTAACTATCCTTGGGCATATGATGCTTGGTTATGTCAGCAAAG
AATACATTGGTTACCTGAAGAGGTACCACTTGCAGATGATGTTCAGGATTGGAAAACAGTGCTTTCGGAAAAAGAAAAAA
ATCTTTTAGTTCAGATTTTTCGTTTTTTTACTCAGGCTGATATTGAAGTGAACAATTGTTACATGAAACATTATTCAAAT
ATTTTTAAACCTACAGAAGTATGTATGATGCTTTCTGCATTTTCAAATATGGAAACTATACATATAGCTGCTTATTCGTA
TTTACTGGATACTATTGGAATGCCTGAAATTGAATACCAAGCATTTTTAAAATTTGAGGTAATGAAGAAGAAATATGATT
ACATGCAAAGGTTTGAGGAATGTAGGAAAAATGATAAAAGACATGTAGCAAAAACTTTAGCAGTTTTTGGTGCTTTTACA
GAGGGGTTGCAGTTGTTTGCTTCATTTGCAATCTTATTAAATTTTCAACGTTTTGGAAAAATGAAAGGTATGGGACAGAT
TATAGCTTGGTCTGTAAGAGATGAAACATTACATACGGAATCAATAATCAGATTGTTTAATACTTTTATTCAAGAGAACT
ATGAAATATGGGATGATTCATTAAAACAAGAATTATATGAAGCGTGCAGAATAATTGTTTCATTAGAAGATGAGTTTATT
AACTTAGCATTTGCTTGTGGAGATGTTGAAGGTTTGTCTTCTAAAGAAGTTAAAGAATATATTAGGTATATTGCAAATAG
AAGATTATTGCAATTAAATCTAGAACCTATATATGATACAAATAAAAACCCTTTATTATGGTTGGATGAAATTTTAAATG
GGGTAGAGCATACTAATTTTTTTGAAAATAGAGTTACAGAATATGCTCGTGCTGCTACAGAAGGAACATGGGAAGAAGCA
TTTGAAGATCATTGA

Upstream 100 bases:

>100_bases
GTAAAAAAATCTTAGTTGATTTTTATGGTAAATAGTTGTTATAGTTTTCCTGGATTTAGCTTGTGATCCATGTTGTTTTT
TTGTTCAGTAGTACTTAGAG

Downstream 100 bases:

>100_bases
TATTAAAGTCATTTTAAGGTATATAGAATTGTATTAATGTAATCTGGACTTTTTTTACTTCTGTAAAAAATAATGTATAA
GTTTGTTATATTCAAACTTT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSLLDSRPIYKPFNYPWAYDAWLCQQRIHWLPEEVPLADDVQDWKTVLSEKEKNLLVQIFRFFTQADIEVNNCYMKHYSN
IFKPTEVCMMLSAFSNMETIHIAAYSYLLDTIGMPEIEYQAFLKFEVMKKKYDYMQRFEECRKNDKRHVAKTLAVFGAFT
EGLQLFASFAILLNFQRFGKMKGMGQIIAWSVRDETLHTESIIRLFNTFIQENYEIWDDSLKQELYEACRIIVSLEDEFI
NLAFACGDVEGLSSKEVKEYIRYIANRRLLQLNLEPIYDTNKNPLLWLDEILNGVEHTNFFENRVTEYARAATEGTWEEA
FEDH

Sequences:

>Translated_324_residues
MSLLDSRPIYKPFNYPWAYDAWLCQQRIHWLPEEVPLADDVQDWKTVLSEKEKNLLVQIFRFFTQADIEVNNCYMKHYSN
IFKPTEVCMMLSAFSNMETIHIAAYSYLLDTIGMPEIEYQAFLKFEVMKKKYDYMQRFEECRKNDKRHVAKTLAVFGAFT
EGLQLFASFAILLNFQRFGKMKGMGQIIAWSVRDETLHTESIIRLFNTFIQENYEIWDDSLKQELYEACRIIVSLEDEFI
NLAFACGDVEGLSSKEVKEYIRYIANRRLLQLNLEPIYDTNKNPLLWLDEILNGVEHTNFFENRVTEYARAATEGTWEEA
FEDH
>Mature_323_residues
SLLDSRPIYKPFNYPWAYDAWLCQQRIHWLPEEVPLADDVQDWKTVLSEKEKNLLVQIFRFFTQADIEVNNCYMKHYSNI
FKPTEVCMMLSAFSNMETIHIAAYSYLLDTIGMPEIEYQAFLKFEVMKKKYDYMQRFEECRKNDKRHVAKTLAVFGAFTE
GLQLFASFAILLNFQRFGKMKGMGQIIAWSVRDETLHTESIIRLFNTFIQENYEIWDDSLKQELYEACRIIVSLEDEFIN
LAFACGDVEGLSSKEVKEYIRYIANRRLLQLNLEPIYDTNKNPLLWLDEILNGVEHTNFFENRVTEYARAATEGTWEEAF
EDH

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=307, Percent_Identity=28.6644951140065, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI4557845, Length=289, Percent_Identity=29.4117647058824, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI260064013, Length=286, Percent_Identity=29.7202797202797, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI289177074, Length=307, Percent_Identity=28.6644951140065, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI289177076, Length=286, Percent_Identity=28.6713286713287, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1789031, Length=301, Percent_Identity=24.5847176079734, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17551968, Length=318, Percent_Identity=28.3018867924528, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6322434, Length=327, Percent_Identity=24.7706422018349, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI18079279, Length=308, Percent_Identity=27.9220779220779, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 38417; Mature: 38286

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure 
SLLDSRPIYKPFNYPWAYDAWLCQQRIHWLPEEVPLADDVQDWKTVLSEKEKNLLVQIF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RFFTQADIEVNNCYMKHYSNIFKPTEVCMMLSAFSNMETIHIAAYSYLLDTIGMPEIEYQ
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHH
AFLKFEVMKKKYDYMQRFEECRKNDKRHVAKTLAVFGAFTEGLQLFASFAILLNFQRFGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
MKGMGQIIAWSVRDETLHTESIIRLFNTFIQENYEIWDDSLKQELYEACRIIVSLEDEFI
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLAFACGDVEGLSSKEVKEYIRYIANRRLLQLNLEPIYDTNKNPLLWLDEILNGVEHTNF
HHHHEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHH
FENRVTEYARAATEGTWEEAFEDH
HHHHHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]