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The map label for this gene is cysS

Identifier: 88658290

GI number: 88658290

Start: 776643

End: 778022

Strand: Reverse

Name: cysS

Synonym: ECH_0768

Alternate gene names: 88658290

Gene position: 778022-776643 (Counterclockwise)

Preceding gene: 88657791

Following gene: 88658048

Centisome position: 66.14

GC content: 32.03

Gene sequence:

>1380_bases
ATGATCATATATAATACTTTAACTGGCATTAAAGAACCTTTTGTACCTTTTAGCATTGATAATGTAAGGGTTTATGTTTG
TGGTCCTACAGTCTATGATTTTGCACATATTGGAAATGCTAGGTCAGTAGTTATATATGATGTGCTGTTTAGGTTATTGA
AGTTGCTATATCCTAAAGTAACGTATGTAAGAAACATTACTGATATTGATGATAAGATTATTAATGTTGCACAAAATACT
AACCAAAGCATATATGATGTTACTGCAATTTATATAAAAGCGTTTCATGAAGATATGAGAATGCTGAATTGTTTGGAACC
TACTTATGAGCCAAGAGCGACTGACAACATAGATGTTATAATAAGTTTAATACAGAAGTTGGTAGATTCTGGTCATGCGT
ATGTATATAATGGTAGTGTGTTCTTTAATATAGAGTCTTATTCTTCTTATGGTCAGTTATCTGGGCGTAATATCTCACAA
CTTATTTATGGTAGTAGAATAGATATAGAGGTAGGTAAAAAGCATCCAGGGGATTTTGTATTATGGAAGCCTGCAACTGA
AATAGATAATCAATTAATGAGTTGCTGGTCAAGTCCTTGGGGGTTAGGAAGACCAGGATGGCACATAGAATGTTCAGCTA
TGAGTTATCGTTATTTAAAGGAAAATTTTGATATTCATGGTGGTGGAGCAGATTTACAATTTCCTCACCATGAAAATGAA
ATAGCACAGAGTTGTTGTGCTTTTCCTGATAGCCGTTATGCTAAATATTGGGTACATAATGGGTTTCTCACAGTTAATGA
TGAGAAAATGAGTAAATCTTTAGGTAATGTTTTAACAGTTAGGCAGTTATTGAATAGTGGGATTACAGGTGAATTGATAA
GGTATGTGTTATTGAGTACTCATTATAGAAAACCTTTAGATTGGAATAGTCAAATGGTGGCTAGTGCTCAAGAATCACTT
AATCGTATGTATACTGCTTTGAGTTCTACAAATGTTGATTTATCGTTGGATGATAGTGTAGAGTTATCGAGTCATGTTAT
TGATTGTTTAAAAGATGATATTAATACTCCAAAAGCTATTTCAATGTTGCATGAAATGGTGACAGAGATTAATAAAACAG
CTGATATGATAGAAAAGATATCTTTAACGAAAACTCTTATTAAGACTGCAAATTTTTTAGGTATCTTACAGCATTCTTGG
CAAGATTGGTTTAAAATTGATGATAATAATCAAGAAATAAGTCAATTGATAGATGAAAGAAAAAAAGCGAGAGCCAATAA
TGATTTTAAAAGGGCAGATGATATACGTCAGCTTTTATTAAATCGTGGAATAGTTCTTTCTGACAATAAAGATGGAACTA
CGCATTGGTATAAGAAATAA

Upstream 100 bases:

>100_bases
GAGTAATAGTATCATTGTTTGGTATTATTGTTACTCTTATGGTAGTTATTCTGGGTCCCAAACTTGTTGTAAGTATGTTT
TCTTTTTTATCTAAATTTAC

Downstream 100 bases:

>100_bases
TAAAATAACAAGTGAATTTTTTTATAACTCTGCGTTATAAAGTTTTATCTTGAATACATATACAGAGATTATAGTAAAAT
ATGAAGTAAATATTTGCTAA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MIIYNTLTGIKEPFVPFSIDNVRVYVCGPTVYDFAHIGNARSVVIYDVLFRLLKLLYPKVTYVRNITDIDDKIINVAQNT
NQSIYDVTAIYIKAFHEDMRMLNCLEPTYEPRATDNIDVIISLIQKLVDSGHAYVYNGSVFFNIESYSSYGQLSGRNISQ
LIYGSRIDIEVGKKHPGDFVLWKPATEIDNQLMSCWSSPWGLGRPGWHIECSAMSYRYLKENFDIHGGGADLQFPHHENE
IAQSCCAFPDSRYAKYWVHNGFLTVNDEKMSKSLGNVLTVRQLLNSGITGELIRYVLLSTHYRKPLDWNSQMVASAQESL
NRMYTALSSTNVDLSLDDSVELSSHVIDCLKDDINTPKAISMLHEMVTEINKTADMIEKISLTKTLIKTANFLGILQHSW
QDWFKIDDNNQEISQLIDERKKARANNDFKRADDIRQLLLNRGIVLSDNKDGTTHWYKK

Sequences:

>Translated_459_residues
MIIYNTLTGIKEPFVPFSIDNVRVYVCGPTVYDFAHIGNARSVVIYDVLFRLLKLLYPKVTYVRNITDIDDKIINVAQNT
NQSIYDVTAIYIKAFHEDMRMLNCLEPTYEPRATDNIDVIISLIQKLVDSGHAYVYNGSVFFNIESYSSYGQLSGRNISQ
LIYGSRIDIEVGKKHPGDFVLWKPATEIDNQLMSCWSSPWGLGRPGWHIECSAMSYRYLKENFDIHGGGADLQFPHHENE
IAQSCCAFPDSRYAKYWVHNGFLTVNDEKMSKSLGNVLTVRQLLNSGITGELIRYVLLSTHYRKPLDWNSQMVASAQESL
NRMYTALSSTNVDLSLDDSVELSSHVIDCLKDDINTPKAISMLHEMVTEINKTADMIEKISLTKTLIKTANFLGILQHSW
QDWFKIDDNNQEISQLIDERKKARANNDFKRADDIRQLLLNRGIVLSDNKDGTTHWYKK
>Mature_459_residues
MIIYNTLTGIKEPFVPFSIDNVRVYVCGPTVYDFAHIGNARSVVIYDVLFRLLKLLYPKVTYVRNITDIDDKIINVAQNT
NQSIYDVTAIYIKAFHEDMRMLNCLEPTYEPRATDNIDVIISLIQKLVDSGHAYVYNGSVFFNIESYSSYGQLSGRNISQ
LIYGSRIDIEVGKKHPGDFVLWKPATEIDNQLMSCWSSPWGLGRPGWHIECSAMSYRYLKENFDIHGGGADLQFPHHENE
IAQSCCAFPDSRYAKYWVHNGFLTVNDEKMSKSLGNVLTVRQLLNSGITGELIRYVLLSTHYRKPLDWNSQMVASAQESL
NRMYTALSSTNVDLSLDDSVELSSHVIDCLKDDINTPKAISMLHEMVTEINKTADMIEKISLTKTLIKTANFLGILQHSW
QDWFKIDDNNQEISQLIDERKKARANNDFKRADDIRQLLLNRGIVLSDNKDGTTHWYKK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=312, Percent_Identity=40.7051282051282, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI10835051, Length=325, Percent_Identity=32.6153846153846, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI21269877, Length=251, Percent_Identity=37.4501992031873, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI303304987, Length=251, Percent_Identity=37.4501992031873, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI62240992, Length=251, Percent_Identity=37.4501992031873, Blast_Score=172, Evalue=5e-43,
Organism=Escherichia coli, GI1786737, Length=465, Percent_Identity=45.1612903225806, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17509795, Length=368, Percent_Identity=29.6195652173913, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17509797, Length=368, Percent_Identity=29.6195652173913, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324082, Length=324, Percent_Identity=33.9506172839506, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI19922218, Length=505, Percent_Identity=32.6732673267327, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI19922364, Length=332, Percent_Identity=31.6265060240964, Blast_Score=171, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_EHRCR (Q2GG67)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507566.1
- ProteinModelPortal:   Q2GG67
- SMR:   Q2GG67
- STRING:   Q2GG67
- GeneID:   3927265
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0768
- TIGR:   ECH_0768
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   VVKIYCC
- PhylomeDB:   Q2GG67
- ProtClustDB:   PRK00260
- BioCyc:   ECHA205920:ECH_0768-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52510; Mature: 52510

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 272-272

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIYNTLTGIKEPFVPFSIDNVRVYVCGPTVYDFAHIGNARSVVIYDVLFRLLKLLYPKV
CEEECCHHCCCCCCCCEEECCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCHH
TYVRNITDIDDKIINVAQNTNQSIYDVTAIYIKAFHEDMRMLNCLEPTYEPRATDNIDVI
HHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
ISLIQKLVDSGHAYVYNGSVFFNIESYSSYGQLSGRNISQLIYGSRIDIEVGKKHPGDFV
HHHHHHHHHCCCEEEEECEEEEEECCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCEE
LWKPATEIDNQLMSCWSSPWGLGRPGWHIECSAMSYRYLKENFDIHGGGADLQFPHHENE
EECCHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
IAQSCCAFPDSRYAKYWVHNGFLTVNDEKMSKSLGNVLTVRQLLNSGITGELIRYVLLST
HHHHHHCCCCCCCCEEEEECCEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
HYRKPLDWNSQMVASAQESLNRMYTALSSTNVDLSLDDSVELSSHVIDCLKDDINTPKAI
HCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHH
SMLHEMVTEINKTADMIEKISLTKTLIKTANFLGILQHSWQDWFKIDDNNQEISQLIDER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHH
KKARANNDFKRADDIRQLLLNRGIVLSDNKDGTTHWYKK
HHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MIIYNTLTGIKEPFVPFSIDNVRVYVCGPTVYDFAHIGNARSVVIYDVLFRLLKLLYPKV
CEEECCHHCCCCCCCCEEECCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCHH
TYVRNITDIDDKIINVAQNTNQSIYDVTAIYIKAFHEDMRMLNCLEPTYEPRATDNIDVI
HHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
ISLIQKLVDSGHAYVYNGSVFFNIESYSSYGQLSGRNISQLIYGSRIDIEVGKKHPGDFV
HHHHHHHHHCCCEEEEECEEEEEECCCCCCCCCCCCCHHHHHCCCEEEEEECCCCCCCEE
LWKPATEIDNQLMSCWSSPWGLGRPGWHIECSAMSYRYLKENFDIHGGGADLQFPHHENE
EECCHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
IAQSCCAFPDSRYAKYWVHNGFLTVNDEKMSKSLGNVLTVRQLLNSGITGELIRYVLLST
HHHHHHCCCCCCCCEEEEECCEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
HYRKPLDWNSQMVASAQESLNRMYTALSSTNVDLSLDDSVELSSHVIDCLKDDINTPKAI
HCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHH
SMLHEMVTEINKTADMIEKISLTKTLIKTANFLGILQHSWQDWFKIDDNNQEISQLIDER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHH
KKARANNDFKRADDIRQLLLNRGIVLSDNKDGTTHWYKK
HHHHCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA