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The map label for this gene is pdxJ

Identifier: 88657971

GI number: 88657971

Start: 819291

End: 820010

Strand: Reverse

Name: pdxJ

Synonym: ECH_0805

Alternate gene names: 88657971

Gene position: 820010-819291 (Counterclockwise)

Preceding gene: 88658591

Following gene: 88657928

Centisome position: 69.71

GC content: 33.61

Gene sequence:

>720_bases
ATGTCAGATGTTGCATTGGGAGTAAATATTGATCATGTTGCAACGTTGCGTAATGCAAGAAATGTTGATTATCCAGATAT
TGTGGAAGTAGCAAATATAGCAGTAAGCAATGGTGCAGATTTTATCACTGTGCATTTAAGAGAAGATCGTAGGCATATAA
AGGATGATGATGTTTTTAGATTGAAAAATAGTTTAAAAGTTCCACTAAACTTAGAAATTGCCCCAACTGATGAGATGTTA
TCAATAGCAATAAAGGTAAGACCAAAATGTGTCTGTTTAGTTCCTGAAAAAAGACAAGAACTTACAACAGAAGGGGGACT
AGATGTAAAACGTGCTTTTTCTTATTTAATATCATTTATTGAAAAACTACATACTTACAACATAGATGTTACACTATTTA
TTGAACCTGATGTAGACCAAATTGATCAAGCAAAAAAACTATCTGCTGATAACGTAGAGCTACATACTGGAAAATACTGT
AACAATACTACACAAAGTGAACTATCTCAAGTAATAAAAGCTGCAGAATACTGCTACCAACGAAACATAGGCTGTCATGC
TGGACACGGCTTAAATTATCAAAGTGCTGCTATTATAGCAAAAGTACCATACATATCAGCACTTAACATAGGGCATTTTT
TAATTTGTGAATCTGTACTACATGGTATAGGTACATCCGTTCATAAAATGAAAAAGGCCATTGCTAGCGCTCCTAATTAA

Upstream 100 bases:

>100_bases
CTCAGATTAGAGGATATGCTTTAAATAAAATATAGTATTTGTGTAAAATTTTATTGATAATATATAATGTATTTTGGTAG
TAGATATATGAGAGGTGCTT

Downstream 100 bases:

>100_bases
TTATCTAAAAGGTTAATAGCATTTGATAATTTTTTGCTTGATCTAAATCTTACTCTTTTCTTAGGAGCTAGTGTTTCTAA
CTTCCCAGTTTTAGGGTTAT

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSDVALGVNIDHVATLRNARNVDYPDIVEVANIAVSNGADFITVHLREDRRHIKDDDVFRLKNSLKVPLNLEIAPTDEML
SIAIKVRPKCVCLVPEKRQELTTEGGLDVKRAFSYLISFIEKLHTYNIDVTLFIEPDVDQIDQAKKLSADNVELHTGKYC
NNTTQSELSQVIKAAEYCYQRNIGCHAGHGLNYQSAAIIAKVPYISALNIGHFLICESVLHGIGTSVHKMKKAIASAPN

Sequences:

>Translated_239_residues
MSDVALGVNIDHVATLRNARNVDYPDIVEVANIAVSNGADFITVHLREDRRHIKDDDVFRLKNSLKVPLNLEIAPTDEML
SIAIKVRPKCVCLVPEKRQELTTEGGLDVKRAFSYLISFIEKLHTYNIDVTLFIEPDVDQIDQAKKLSADNVELHTGKYC
NNTTQSELSQVIKAAEYCYQRNIGCHAGHGLNYQSAAIIAKVPYISALNIGHFLICESVLHGIGTSVHKMKKAIASAPN
>Mature_238_residues
SDVALGVNIDHVATLRNARNVDYPDIVEVANIAVSNGADFITVHLREDRRHIKDDDVFRLKNSLKVPLNLEIAPTDEMLS
IAIKVRPKCVCLVPEKRQELTTEGGLDVKRAFSYLISFIEKLHTYNIDVTLFIEPDVDQIDQAKKLSADNVELHTGKYCN
NTTQSELSQVIKAAEYCYQRNIGCHAGHGLNYQSAAIIAKVPYISALNIGHFLICESVLHGIGTSVHKMKKAIASAPN

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=241, Percent_Identity=46.8879668049792, Blast_Score=218, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_EHRCR (Q2GG30)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507603.1
- ProteinModelPortal:   Q2GG30
- SMR:   Q2GG30
- STRING:   Q2GG30
- GeneID:   3927206
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0805
- TIGR:   ECH_0805
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q2GG30
- ProtClustDB:   PRK05265
- BioCyc:   ECHA205920:ECH_0805-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 26508; Mature: 26376

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: ACT_SITE 45-45 ACT_SITE 72-72 ACT_SITE 189-189 BINDING 9-9 BINDING 20-20 BINDING 47-47 BINDING 52-52 BINDING 102-102 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVALGVNIDHVATLRNARNVDYPDIVEVANIAVSNGADFITVHLREDRRHIKDDDVFR
CCCEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHH
LKNSLKVPLNLEIAPTDEMLSIAIKVRPKCVCLVPEKRQELTTEGGLDVKRAFSYLISFI
HHCCCCCCEEEEECCCHHEEEEEEEECCEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHH
EKLHTYNIDVTLFIEPDVDQIDQAKKLSADNVELHTGKYCNNTTQSELSQVIKAAEYCYQ
HHHHHCCCEEEEEECCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
RNIGCHAGHGLNYQSAAIIAKVPYISALNIGHFLICESVLHGIGTSVHKMKKAIASAPN
CCCCCCCCCCCCCCCCEEEEECCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDVALGVNIDHVATLRNARNVDYPDIVEVANIAVSNGADFITVHLREDRRHIKDDDVFR
CCEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHH
LKNSLKVPLNLEIAPTDEMLSIAIKVRPKCVCLVPEKRQELTTEGGLDVKRAFSYLISFI
HHCCCCCCEEEEECCCHHEEEEEEEECCEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHH
EKLHTYNIDVTLFIEPDVDQIDQAKKLSADNVELHTGKYCNNTTQSELSQVIKAAEYCYQ
HHHHHCCCEEEEEECCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
RNIGCHAGHGLNYQSAAIIAKVPYISALNIGHFLICESVLHGIGTSVHKMKKAIASAPN
CCCCCCCCCCCCCCCCEEEEECCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA