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The map label for this gene is tilS [H]

Identifier: 88657904

GI number: 88657904

Start: 1120270

End: 1121574

Strand: Direct

Name: tilS [H]

Synonym: ECH_1097

Alternate gene names: 88657904

Gene position: 1120270-1121574 (Clockwise)

Preceding gene: 88657968

Following gene: 88657587

Centisome position: 95.24

GC content: 29.12

Gene sequence:

>1305_bases
ATGATAAATTTAGGGCTGTTATTTCAAAAAAAAATTCAAAACTTGAATGATAATTTTGCAATTGCAGTTTCTGGTGGAAT
AGATAGCATGACACTATTACATTTATCAGCACGATACTGTAATAAGAACACCCCTATAGTATTAACAGTAAACCATGGCT
TGAGACCTGAAGCAGCACAAGAAGCATTGTTTGTATTTCAACATTCACAGAATTTAAACTTAAAATGCCACATACTAAAT
TGGCATGGTAAATTACCAGAAAGTAATATACAATCTTCTGCTAGACGAATACGATACAAGTTATTACTACAATGGTGTAA
TGAAAATAGAATAAAATATTTAATGGTAGCACATCAGAAAAATGATCAAGCAGAGACTATTATGATTAGGTTAGAAAGAG
GAAGTGGGTTAGACGGATTAGCTGGAATGCAAGAATACACATACTTAAATGGTATATGTATACTTAGGCCACTCCTAAGT
GCTAGCAGGACAGAATTATTACAATATGCAAACAAAAACAATATTACTTGGATAAATGATACTAGTAATAACAATAAAAA
GTACAAAAGAACCCTATACCGCAATATGTTAGAAATAACAGATAATCCTGAAGTATTAATTAATAGACTTTACACAGCAT
CAGAACATATAAAAAGATCTCTAAATTGCATCTTACATTATGTAAGGTTAGCTACTGACCAATGTTTAGAGTTTACCAAC
CTAGGACATATTAATATAAAACTAGATATATTTTTAACACTACCAGAAGAAATATCAATAAGGCTTTTAACATACTCTAT
CATGTCTATTGGTCAACAAAAATATAAGCCAAGGTATAGCAAACTTAGTAATATATTTTCTAAAATAAAAAATAATAAAC
ACAAGATAGCTCAAACTTTATGTGGTTGTAAAATCACAACAAATCATAATAACATTATCTCCATTACTAGAGAGGTATCA
GCAATACAAGAACTCACTATTAACTCTGCTACAAATATAGTAATAGAATGGGACAATAGATTCAAAATAAGTATTGTCAA
TTCTAATGCTGACAATGTTACTGTTTCTAGTTTAAATAATAATTATATTCCAGAAGAGCTAAAAAAGTTACACAGAGAAG
CAGTACGTTGTTTACCTATACTAAAACATAAAAATAAAATTGTTGCTTACCCCCTACAAAATAATAGTAATAATAACTAT
ATTGATCAAGTAAAGTCATCTATTATAATAAAAGAGGTGTTGGTCAAACAAAATTTTATCAATTTAACTTACAGTGAATT
TATCAGCAAGGAGCCTCTGCTATGA

Upstream 100 bases:

>100_bases
GTGGTCTAAAGCGCACGCCTGGAAAGTGTGTATGCAGTAATCCTGTATCGAGGGTTCGAATCCCTCTCTTTCCGCCATGT
GTTTTTATTATTGGGGATTA

Downstream 100 bases:

>100_bases
GAAAAATAATTGAAGGGTTAGCAATTTGGATTATAGTAGTTGTTCTGGTTGCTGCAGCATATGTTCAATTTAATGATAAA
ATCATTAACGGTAATACCAT

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MINLGLLFQKKIQNLNDNFAIAVSGGIDSMTLLHLSARYCNKNTPIVLTVNHGLRPEAAQEALFVFQHSQNLNLKCHILN
WHGKLPESNIQSSARRIRYKLLLQWCNENRIKYLMVAHQKNDQAETIMIRLERGSGLDGLAGMQEYTYLNGICILRPLLS
ASRTELLQYANKNNITWINDTSNNNKKYKRTLYRNMLEITDNPEVLINRLYTASEHIKRSLNCILHYVRLATDQCLEFTN
LGHINIKLDIFLTLPEEISIRLLTYSIMSIGQQKYKPRYSKLSNIFSKIKNNKHKIAQTLCGCKITTNHNNIISITREVS
AIQELTINSATNIVIEWDNRFKISIVNSNADNVTVSSLNNNYIPEELKKLHREAVRCLPILKHKNKIVAYPLQNNSNNNY
IDQVKSSIIIKEVLVKQNFINLTYSEFISKEPLL

Sequences:

>Translated_434_residues
MINLGLLFQKKIQNLNDNFAIAVSGGIDSMTLLHLSARYCNKNTPIVLTVNHGLRPEAAQEALFVFQHSQNLNLKCHILN
WHGKLPESNIQSSARRIRYKLLLQWCNENRIKYLMVAHQKNDQAETIMIRLERGSGLDGLAGMQEYTYLNGICILRPLLS
ASRTELLQYANKNNITWINDTSNNNKKYKRTLYRNMLEITDNPEVLINRLYTASEHIKRSLNCILHYVRLATDQCLEFTN
LGHINIKLDIFLTLPEEISIRLLTYSIMSIGQQKYKPRYSKLSNIFSKIKNNKHKIAQTLCGCKITTNHNNIISITREVS
AIQELTINSATNIVIEWDNRFKISIVNSNADNVTVSSLNNNYIPEELKKLHREAVRCLPILKHKNKIVAYPLQNNSNNNY
IDQVKSSIIIKEVLVKQNFINLTYSEFISKEPLL
>Mature_434_residues
MINLGLLFQKKIQNLNDNFAIAVSGGIDSMTLLHLSARYCNKNTPIVLTVNHGLRPEAAQEALFVFQHSQNLNLKCHILN
WHGKLPESNIQSSARRIRYKLLLQWCNENRIKYLMVAHQKNDQAETIMIRLERGSGLDGLAGMQEYTYLNGICILRPLLS
ASRTELLQYANKNNITWINDTSNNNKKYKRTLYRNMLEITDNPEVLINRLYTASEHIKRSLNCILHYVRLATDQCLEFTN
LGHINIKLDIFLTLPEEISIRLLTYSIMSIGQQKYKPRYSKLSNIFSKIKNNKHKIAQTLCGCKITTNHNNIISITREVS
AIQELTINSATNIVIEWDNRFKISIVNSNADNVTVSSLNNNYIPEELKKLHREAVRCLPILKHKNKIVAYPLQNNSNNNY
IDQVKSSIIIKEVLVKQNFINLTYSEFISKEPLL

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=186, Percent_Identity=32.258064516129, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 49994; Mature: 49994

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLGLLFQKKIQNLNDNFAIAVSGGIDSMTLLHLSARYCNKNTPIVLTVNHGLRPEAAQ
CCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHH
EALFVFQHSQNLNLKCHILNWHGKLPESNIQSSARRIRYKLLLQWCNENRIKYLMVAHQK
HHEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECC
NDQAETIMIRLERGSGLDGLAGMQEYTYLNGICILRPLLSASRTELLQYANKNNITWIND
CCCCEEEEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
TSNNNKKYKRTLYRNMLEITDNPEVLINRLYTASEHIKRSLNCILHYVRLATDQCLEFTN
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LGHINIKLDIFLTLPEEISIRLLTYSIMSIGQQKYKPRYSKLSNIFSKIKNNKHKIAQTL
CCEEEEEEEEEEECCCHHEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
CGCKITTNHNNIISITREVSAIQELTINSATNIVIEWDNRFKISIVNSNADNVTVSSLNN
HCCEEEECCCCEEEEHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEECCCCEEEEEECCC
NYIPEELKKLHREAVRCLPILKHKNKIVAYPLQNNSNNNYIDQVKSSIIIKEVLVKQNFI
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
NLTYSEFISKEPLL
CEEHHHHCCCCCCC
>Mature Secondary Structure
MINLGLLFQKKIQNLNDNFAIAVSGGIDSMTLLHLSARYCNKNTPIVLTVNHGLRPEAAQ
CCCCHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHH
EALFVFQHSQNLNLKCHILNWHGKLPESNIQSSARRIRYKLLLQWCNENRIKYLMVAHQK
HHEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECC
NDQAETIMIRLERGSGLDGLAGMQEYTYLNGICILRPLLSASRTELLQYANKNNITWIND
CCCCEEEEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
TSNNNKKYKRTLYRNMLEITDNPEVLINRLYTASEHIKRSLNCILHYVRLATDQCLEFTN
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LGHINIKLDIFLTLPEEISIRLLTYSIMSIGQQKYKPRYSKLSNIFSKIKNNKHKIAQTL
CCEEEEEEEEEEECCCHHEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
CGCKITTNHNNIISITREVSAIQELTINSATNIVIEWDNRFKISIVNSNADNVTVSSLNN
HCCEEEECCCCEEEEHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEECCCCEEEEEECCC
NYIPEELKKLHREAVRCLPILKHKNKIVAYPLQNNSNNNYIDQVKSSIIIKEVLVKQNFI
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
NLTYSEFISKEPLL
CEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA