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The map label for this gene is dnaX [H]

Identifier: 88657800

GI number: 88657800

Start: 11920

End: 13437

Strand: Direct

Name: dnaX [H]

Synonym: ECH_0014

Alternate gene names: 88657800

Gene position: 11920-13437 (Clockwise)

Preceding gene: 88657880

Following gene: 88658046

Centisome position: 1.01

GC content: 29.05

Gene sequence:

>1518_bases
TTGGGTTGTATTGCCCATTTTTTATGTTACCATTCTACAATGAGTTTTGCATTAAAGTATAGACCTAGTAATTTTAAAGA
TCTCATAGGGCAAGAGGTTTTAGTTCGTGTTCTACATAATGCATTTCACTTAAATAAAATACCAGAATCTATACTTTTGA
CAGGTATTAGTGGTGTAGGAAAAACTACCGCTGCAAGGATAATATCTATGTGCTTAAACTGTATTTCTGGACCAACGTCT
GATCCATGTAATACATGTCAAAATTGTATATCTATAAAAAATTTCAACCATCCAGATGTAATAGAAATAGACGCAGCAAG
CAACACAAGCATAGAAGATGTTAAAGTGATTTTAGAGAATTCGCGCTACATGCCAATTAGTTCAAAATTCAAAGTATACA
TAATAGACGAAGTACATATGTTATCTAATAGTGCATTTAATGCCCTACTGAAAGTACTAGAAGAACCTGCACCTTATGTA
AAATTCATTTTAGCAACAACAGAAGTAAAAAAAATACCTGTAACTATTATATCTAGATGTCAGAGGTTTGACTTTCAAAG
GATATCCACAGATAAACTTGTAGATCATCTAAAAGTCGTGGCAAAAAAAGAAAATGTATCCTTCGATGAAGAAAGTATAA
AATTAATAGCATATAATGCTGATGGATCTATACGCAATGCTCTATCTTTATTGGAGCAAGCTGCTATATACAGTAATAAA
ATCTTATCTGAAAACATCACAAAAGAAATGCTGGGTTATGTTAGTAGACATACATTACTAGAAATAATAGAACCATTAAT
TATAGGGAATGCTACAGAAGCTCTAAATAAATTTGAAGAAGCATGTAACTCGTATAACTCTGTTATAATTTTAGATAATA
TGTTGCAGATTATTTATGAAATATGTTACTTCTCTATAACAAAAAAAGACAACTCTACATCTGATAACAGTATTATAACA
TATATAAAAAACGAAAAGATTTTATCTCCTACATTTCTATCAAGGTTATGGCAAATGCTTTTTAAAGGTATACAGGAAGT
AAAGTCATCTAGCTGTATAAAGCAGGCTGCAAATATGCTAATCATAAGGTTATGTTATTTGTCATCTTTACCATCTCCAA
AACAAATAATAGATAAGATTCAAGGAAAAATAAACAACACATCAGATATTAACCGTAAAGCATTACTTGAATTGTTAAAA
AGTAATAACATGCAAGACTTATATAACCAAATTTACGAATCTGAGTCAGAAGTAAAAAATTATAAAGATGAAGCATTAAA
TATAGAAACTAACCAGGCATTATCTAAAGATTTTCATGAGGATTCACTAAATTTTTTCAATAATTCAACAAATAAAAAAT
GTAATGTACCCATTGAACAAAATTTTGATATAAATAAAAGTAGTAATCCATGTTATAATCAACATCTTGATCCAACTGTT
TGCAATATATTACAAGAGTTCGATGGAGCAAAAGTTATAAATATAGAAAACATAAAAAAGAACAACAATAAGGAGTGA

Upstream 100 bases:

>100_bases
TGATATTTCTTTAAGTTAAATTATAATGCAAAGGGTAATATGAGCAAAGGGCTTGTGAAGCCGGTCAGGTTAGGAATAAA
GCAGCCGTAGCATTGTTTCT

Downstream 100 bases:

>100_bases
GCTATGTACTCTAGCGATCAATTATCTAACTTACAAGAGATGATAAAAAAGAAGTTTTCCGACTTTCAAGAACACCAAAA
ATCTCAAATATTTGAAGGTT

Product: DNA polymerase III, subunits gamma and tau

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MGCIAHFLCYHSTMSFALKYRPSNFKDLIGQEVLVRVLHNAFHLNKIPESILLTGISGVGKTTAARIISMCLNCISGPTS
DPCNTCQNCISIKNFNHPDVIEIDAASNTSIEDVKVILENSRYMPISSKFKVYIIDEVHMLSNSAFNALLKVLEEPAPYV
KFILATTEVKKIPVTIISRCQRFDFQRISTDKLVDHLKVVAKKENVSFDEESIKLIAYNADGSIRNALSLLEQAAIYSNK
ILSENITKEMLGYVSRHTLLEIIEPLIIGNATEALNKFEEACNSYNSVIILDNMLQIIYEICYFSITKKDNSTSDNSIIT
YIKNEKILSPTFLSRLWQMLFKGIQEVKSSSCIKQAANMLIIRLCYLSSLPSPKQIIDKIQGKINNTSDINRKALLELLK
SNNMQDLYNQIYESESEVKNYKDEALNIETNQALSKDFHEDSLNFFNNSTNKKCNVPIEQNFDINKSSNPCYNQHLDPTV
CNILQEFDGAKVINIENIKKNNNKE

Sequences:

>Translated_505_residues
MGCIAHFLCYHSTMSFALKYRPSNFKDLIGQEVLVRVLHNAFHLNKIPESILLTGISGVGKTTAARIISMCLNCISGPTS
DPCNTCQNCISIKNFNHPDVIEIDAASNTSIEDVKVILENSRYMPISSKFKVYIIDEVHMLSNSAFNALLKVLEEPAPYV
KFILATTEVKKIPVTIISRCQRFDFQRISTDKLVDHLKVVAKKENVSFDEESIKLIAYNADGSIRNALSLLEQAAIYSNK
ILSENITKEMLGYVSRHTLLEIIEPLIIGNATEALNKFEEACNSYNSVIILDNMLQIIYEICYFSITKKDNSTSDNSIIT
YIKNEKILSPTFLSRLWQMLFKGIQEVKSSSCIKQAANMLIIRLCYLSSLPSPKQIIDKIQGKINNTSDINRKALLELLK
SNNMQDLYNQIYESESEVKNYKDEALNIETNQALSKDFHEDSLNFFNNSTNKKCNVPIEQNFDINKSSNPCYNQHLDPTV
CNILQEFDGAKVINIENIKKNNNKE
>Mature_504_residues
GCIAHFLCYHSTMSFALKYRPSNFKDLIGQEVLVRVLHNAFHLNKIPESILLTGISGVGKTTAARIISMCLNCISGPTSD
PCNTCQNCISIKNFNHPDVIEIDAASNTSIEDVKVILENSRYMPISSKFKVYIIDEVHMLSNSAFNALLKVLEEPAPYVK
FILATTEVKKIPVTIISRCQRFDFQRISTDKLVDHLKVVAKKENVSFDEESIKLIAYNADGSIRNALSLLEQAAIYSNKI
LSENITKEMLGYVSRHTLLEIIEPLIIGNATEALNKFEEACNSYNSVIILDNMLQIIYEICYFSITKKDNSTSDNSIITY
IKNEKILSPTFLSRLWQMLFKGIQEVKSSSCIKQAANMLIIRLCYLSSLPSPKQIIDKIQGKINNTSDINRKALLELLKS
NNMQDLYNQIYESESEVKNYKDEALNIETNQALSKDFHEDSLNFFNNSTNKKCNVPIEQNFDINKSSNPCYNQHLDPTVC
NILQEFDGAKVINIENIKKNNNKE

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI6677723, Length=235, Percent_Identity=27.2340425531915, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI194306571, Length=230, Percent_Identity=27.8260869565217, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI194306567, Length=230, Percent_Identity=27.8260869565217, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4506491, Length=246, Percent_Identity=25.2032520325203, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI31881687, Length=246, Percent_Identity=25.2032520325203, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI31563534, Length=243, Percent_Identity=25.1028806584362, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1786676, Length=362, Percent_Identity=34.5303867403315, Blast_Score=243, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17541988, Length=233, Percent_Identity=27.8969957081545, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17554730, Length=229, Percent_Identity=23.5807860262009, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71986063, Length=263, Percent_Identity=24.3346007604563, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322528, Length=291, Percent_Identity=23.7113402061856, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324039, Length=221, Percent_Identity=23.9819004524887, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324478, Length=240, Percent_Identity=21.6666666666667, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI18859927, Length=297, Percent_Identity=25.2525252525253, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921076, Length=345, Percent_Identity=24.6376811594203, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17647857, Length=242, Percent_Identity=26.4462809917355, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR022754
- InterPro:   IPR012763
- InterPro:   IPR021029 [H]

Pfam domain/function: PF00004 AAA; PF12169 DNA_pol3_gamma3; PF12170 DNA_pol3_tau_5 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 57195; Mature: 57064

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGCIAHFLCYHSTMSFALKYRPSNFKDLIGQEVLVRVLHNAFHLNKIPESILLTGISGVG
CHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
KTTAARIISMCLNCISGPTSDPCNTCQNCISIKNFNHPDVIEIDAASNTSIEDVKVILEN
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHC
SRYMPISSKFKVYIIDEVHMLSNSAFNALLKVLEEPAPYVKFILATTEVKKIPVTIISRC
CCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
QRFDFQRISTDKLVDHLKVVAKKENVSFDEESIKLIAYNADGSIRNALSLLEQAAIYSNK
HHCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
ILSENITKEMLGYVSRHTLLEIIEPLIIGNATEALNKFEEACNSYNSVIILDNMLQIIYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHH
ICYFSITKKDNSTSDNSIITYIKNEKILSPTFLSRLWQMLFKGIQEVKSSSCIKQAANML
HHHHEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIRLCYLSSLPSPKQIIDKIQGKINNTSDINRKALLELLKSNNMQDLYNQIYESESEVKN
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHHH
YKDEALNIETNQALSKDFHEDSLNFFNNSTNKKCNVPIEQNFDINKSSNPCYNQHLDPTV
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHH
CNILQEFDGAKVINIENIKKNNNKE
HHHHHHCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
GCIAHFLCYHSTMSFALKYRPSNFKDLIGQEVLVRVLHNAFHLNKIPESILLTGISGVG
HHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCC
KTTAARIISMCLNCISGPTSDPCNTCQNCISIKNFNHPDVIEIDAASNTSIEDVKVILEN
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHC
SRYMPISSKFKVYIIDEVHMLSNSAFNALLKVLEEPAPYVKFILATTEVKKIPVTIISRC
CCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
QRFDFQRISTDKLVDHLKVVAKKENVSFDEESIKLIAYNADGSIRNALSLLEQAAIYSNK
HHCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
ILSENITKEMLGYVSRHTLLEIIEPLIIGNATEALNKFEEACNSYNSVIILDNMLQIIYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHH
ICYFSITKKDNSTSDNSIITYIKNEKILSPTFLSRLWQMLFKGIQEVKSSSCIKQAANML
HHHHEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIRLCYLSSLPSPKQIIDKIQGKINNTSDINRKALLELLKSNNMQDLYNQIYESESEVKN
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHHH
YKDEALNIETNQALSKDFHEDSLNFFNNSTNKKCNVPIEQNFDINKSSNPCYNQHLDPTV
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHH
CNILQEFDGAKVINIENIKKNNNKE
HHHHHHCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]