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The map label for this gene is purN [H]

Identifier: 88657719

GI number: 88657719

Start: 362656

End: 363282

Strand: Direct

Name: purN [H]

Synonym: ECH_0370

Alternate gene names: 88657719

Gene position: 362656-363282 (Clockwise)

Preceding gene: 88658513

Following gene: 88657879

Centisome position: 30.83

GC content: 33.65

Gene sequence:

>627_bases
ATGACGCCGCTTAAGTTAGGTATTTTAATTTCAGGAAGGGGTTCTAATATGCAAGCATTAATTAATGCTTGTGCACAGGA
TGATTTCCCTGCAGAAGTATCCTGTGTGATTTCTAATAACCCTAAAGCAAATGGTTTATTGATTGCACAAAAACAAAATA
TTAAGACTTTTGTAGTACAAGGTCGTCCATTGGATTTTGATTCTATTGATAGTATACTTAGACAACATCAAGTAGATTTA
ATATGCCTTGCAGGGTTTATGAGTATTGTACCTGAGGGCTTTATTAATAAGTGGTTTCATAAAATTATTAATATACATCC
TTCTCTTTTACCGTCTTTTAAAGGATTGAATGCACAGTCTCAAGCATTGAAAGCTGGAGTTAAAATTGCAGGATGTACTG
TGCATTATGTATATCCAGAAGTTGATGGTGGTCCCATTATCGTACAAGCAGCAGTTCCTGTTTTTTCATCGGATAATCTT
ACAGATCTTTCCGAGAGGATATTAAAAATGGAACATATTTGTTATCCTAAAGCTGTAAAGTTGATTGCATTGAATCAGCT
TCAACTTAATGAAAATTTAGCCACATCAGCGGAATTGTTGCATATGTTTTATGATGAAACTATTTAA

Upstream 100 bases:

>100_bases
ATTTTATAAAGATTCTGGAGTACTAGTCAATTTGGGATATAATATGATTTATTTTACGATGCTCTTTGTACATGGTTAAT
ATTATGGGTAACTTAGTTAT

Downstream 100 bases:

>100_bases
TTCTTTTATATGTTACGTAAAATCTGAGTAAAAAATTTGTTTTTATGTATTCTAGTAAGCTTGTAAATTATATATACGTG
TGGTAATACTTTTAAGTTAT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTPLKLGILISGRGSNMQALINACAQDDFPAEVSCVISNNPKANGLLIAQKQNIKTFVVQGRPLDFDSIDSILRQHQVDL
ICLAGFMSIVPEGFINKWFHKIINIHPSLLPSFKGLNAQSQALKAGVKIAGCTVHYVYPEVDGGPIIVQAAVPVFSSDNL
TDLSERILKMEHICYPKAVKLIALNQLQLNENLATSAELLHMFYDETI

Sequences:

>Translated_208_residues
MTPLKLGILISGRGSNMQALINACAQDDFPAEVSCVISNNPKANGLLIAQKQNIKTFVVQGRPLDFDSIDSILRQHQVDL
ICLAGFMSIVPEGFINKWFHKIINIHPSLLPSFKGLNAQSQALKAGVKIAGCTVHYVYPEVDGGPIIVQAAVPVFSSDNL
TDLSERILKMEHICYPKAVKLIALNQLQLNENLATSAELLHMFYDETI
>Mature_207_residues
TPLKLGILISGRGSNMQALINACAQDDFPAEVSCVISNNPKANGLLIAQKQNIKTFVVQGRPLDFDSIDSILRQHQVDLI
CLAGFMSIVPEGFINKWFHKIINIHPSLLPSFKGLNAQSQALKAGVKIAGCTVHYVYPEVDGGPIIVQAAVPVFSSDNLT
DLSERILKMEHICYPKAVKLIALNQLQLNENLATSAELLHMFYDETI

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=193, Percent_Identity=46.6321243523316, Blast_Score=177, Evalue=8e-45,
Organism=Homo sapiens, GI209869995, Length=193, Percent_Identity=46.6321243523316, Blast_Score=177, Evalue=8e-45,
Organism=Homo sapiens, GI209869993, Length=193, Percent_Identity=46.6321243523316, Blast_Score=177, Evalue=8e-45,
Organism=Escherichia coli, GI1788846, Length=194, Percent_Identity=39.6907216494845, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787483, Length=97, Percent_Identity=40.2061855670103, Blast_Score=74, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=188, Percent_Identity=39.8936170212766, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320616, Length=206, Percent_Identity=29.126213592233, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582400, Length=187, Percent_Identity=43.8502673796791, Blast_Score=150, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22811; Mature: 22680

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLKLGILISGRGSNMQALINACAQDDFPAEVSCVISNNPKANGLLIAQKQNIKTFVVQ
CCCEEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCEEEEEE
GRPLDFDSIDSILRQHQVDLICLAGFMSIVPEGFINKWFHKIINIHPSLLPSFKGLNAQS
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCCCCCCCHHH
QALKAGVKIAGCTVHYVYPEVDGGPIIVQAAVPVFSSDNLTDLSERILKMEHICYPKAVK
HHHHCCCEEEEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHEE
LIALNQLQLNENLATSAELLHMFYDETI
EEEEEEEECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TPLKLGILISGRGSNMQALINACAQDDFPAEVSCVISNNPKANGLLIAQKQNIKTFVVQ
CCEEEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCEEEEEE
GRPLDFDSIDSILRQHQVDLICLAGFMSIVPEGFINKWFHKIINIHPSLLPSFKGLNAQS
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCCCCCCCHHH
QALKAGVKIAGCTVHYVYPEVDGGPIIVQAAVPVFSSDNLTDLSERILKMEHICYPKAVK
HHHHCCCEEEEEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHEE
LIALNQLQLNENLATSAELLHMFYDETI
EEEEEEEECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]