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The map label for this gene is thrS [H]

Identifier: 88657649

GI number: 88657649

Start: 3683

End: 5584

Strand: Direct

Name: thrS [H]

Synonym: ECH_0006

Alternate gene names: 88657649

Gene position: 3683-5584 (Clockwise)

Preceding gene: 88658630

Following gene: 88658160

Centisome position: 0.31

GC content: 31.97

Gene sequence:

>1902_bases
ATGATAAACATCCACTTTAGTAACAATTTATGCAAGCAGTTTCATCGTGGAATTAAAGGACATGATATAGTAAATGACCT
ATTTCCAAAATTAAAAAACGAAACAATAGCTGCAAAAGTAAACGGAGAATTATACGATTTATCAAGAGAAATCATAGAAA
ATTGCACTTTTGAAGTCATAACGATAAACAGCGAAGAAGGTCTTGAAATTATACGTCATGATACTGCTCACATCATGGCA
CAAGCCGTGAAAGAAATGTTTCCAGATGTTCAAATAACTATTGGACCAACTATTAAAGACGGTTTTTATTATGATTTCGC
AACAAATCATAACTTTTCTAGTGATGACCTAGAAATAATAGAAAAGAAAATGATAGAAATTATAAATAAAAATGAAAGCT
TTATACGGGAAGTATGGTCTAGAGAAGAAGCTATAAAATTCTTCTCAAGTATAGGAGAAGACTATAAGGTCAAAATAATA
TCTAATATTCCAAGTAATGAGAATATTACTGTTTATAAGCAGGGAAGTTTTACTGATTTATGTCGTGGACCACATGCACC
TTCAACAAAAACATCAAGAGCATTTAAATTAACAAAAGTATCTGGATCTTATTGGCAAGGCAATTCAAATAATGAAAGAT
TACAAAGAATTTATGGTACAGCATGGCGTAATGAAGAAGAATTAAAACTTTACTTAAATAATTTAATAGAAGCAGAAAAA
AGAGATCACAGAAAAATTGGTAGGGAATTAGAGTTGTTTCATATTCAAAATGAAGCATGCGGTCAAATATTTTGGCACAC
AAAAGGGTGGACTATTTATCGTATTATAGAAAATTATATACGAAAAAAACTAGAAAATAATGGATATATAGAAGTAAAAA
CCCCCATATTGTTAAACAAAGAACTTTGGGAAAAATCAGGACATTGGGATAAGTTTCGTGAAAACATGTTTTTAAGTGAA
GCAGAAGATAAAACTTTAGCCATAAAACCAATGAATTGCCCATGCCACATACAGATCTTTAACTCAAAAATTAGAAGTTA
TCGAGATTTACCAATACGAATGGCAGAATTTGGAACATGTCATAGGTATGAAGCATCAGGAGCATTACATGGGTTAATGA
GAGTTCGCGGTTTTACTCAAGATGATGCTCACATTTTCTGTACAGAAAGCCAAATTACATCTGAAGCTCTAAAGTTTTGT
AATTTACTCATAGAAATCTACAAAGATTTTGGTTTCACCGATATTCTAGTAAAATTTTCAGATCGTCCAAAGAACAGAGC
TGGTAGCGATGAAATATGGGATAAAGCTGAAGCAGCATTAAAGAAATCAGTCGAAGTTGCTAACTTAAGTTATGTTTTAA
ACCCTGGAGATGGAGCATTCTATGGACCAAAATTAGAATTTACATTAAAAGATGCTATTGGCAGAGAATGGCAATGTGGA
ACTCTACAAATGGATTTCGTATTACCTGAAAGGTTAGGTGCCTACTATATAGGTAGTGATGGGAAAAAACATCATCCAGT
AATGCTACACCGTGCTATCTTAGGTACTTTTGAAAGGTTCATAGGAATATTAATTGAACATCATTCAGGCAAATTTCCAA
TGTGGTTAGCTCCTATACAATTATCAATATTAACTATCAGTGAAGATTCCATTAACTATGCTAATTCCTTGAAAATAAAA
GCCGAAGAACATAATATAAGAGTTGAACTAGATACTACAAACGAAAAAATAAATTATAAGATACGGAACCATATCCACAA
GAAAGTACCTGTGTTCTGGATAGTAGGTAAAAAAGAAGTTGAAGAAAATTCTGTATCTATACGTTATTTAGAGTCCAATA
AGCAGCACGTTATGCCTATTGATAAAGCGTTGAAAACATTATTAACTTGTGCTAGCATCTAG

Upstream 100 bases:

>100_bases
TGAGTTAAAAAATACAATGTAACACACTCAAAATTAAGATATAATAGCAAAATAAAGTTGTGACTTTAAAGATTAAATAG
TAACTTATGGTTAATATAAT

Downstream 100 bases:

>100_bases
TAAATTTTAGTCAGGAGTGTAAGATTGAAAACAAAAAAGTTCTCTAATAAAAACAAGATTAATGAAATGATCACAGCAAA
AAAAGTAAAGCTTGTTGATC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MINIHFSNNLCKQFHRGIKGHDIVNDLFPKLKNETIAAKVNGELYDLSREIIENCTFEVITINSEEGLEIIRHDTAHIMA
QAVKEMFPDVQITIGPTIKDGFYYDFATNHNFSSDDLEIIEKKMIEIINKNESFIREVWSREEAIKFFSSIGEDYKVKII
SNIPSNENITVYKQGSFTDLCRGPHAPSTKTSRAFKLTKVSGSYWQGNSNNERLQRIYGTAWRNEEELKLYLNNLIEAEK
RDHRKIGRELELFHIQNEACGQIFWHTKGWTIYRIIENYIRKKLENNGYIEVKTPILLNKELWEKSGHWDKFRENMFLSE
AEDKTLAIKPMNCPCHIQIFNSKIRSYRDLPIRMAEFGTCHRYEASGALHGLMRVRGFTQDDAHIFCTESQITSEALKFC
NLLIEIYKDFGFTDILVKFSDRPKNRAGSDEIWDKAEAALKKSVEVANLSYVLNPGDGAFYGPKLEFTLKDAIGREWQCG
TLQMDFVLPERLGAYYIGSDGKKHHPVMLHRAILGTFERFIGILIEHHSGKFPMWLAPIQLSILTISEDSINYANSLKIK
AEEHNIRVELDTTNEKINYKIRNHIHKKVPVFWIVGKKEVEENSVSIRYLESNKQHVMPIDKALKTLLTCASI

Sequences:

>Translated_633_residues
MINIHFSNNLCKQFHRGIKGHDIVNDLFPKLKNETIAAKVNGELYDLSREIIENCTFEVITINSEEGLEIIRHDTAHIMA
QAVKEMFPDVQITIGPTIKDGFYYDFATNHNFSSDDLEIIEKKMIEIINKNESFIREVWSREEAIKFFSSIGEDYKVKII
SNIPSNENITVYKQGSFTDLCRGPHAPSTKTSRAFKLTKVSGSYWQGNSNNERLQRIYGTAWRNEEELKLYLNNLIEAEK
RDHRKIGRELELFHIQNEACGQIFWHTKGWTIYRIIENYIRKKLENNGYIEVKTPILLNKELWEKSGHWDKFRENMFLSE
AEDKTLAIKPMNCPCHIQIFNSKIRSYRDLPIRMAEFGTCHRYEASGALHGLMRVRGFTQDDAHIFCTESQITSEALKFC
NLLIEIYKDFGFTDILVKFSDRPKNRAGSDEIWDKAEAALKKSVEVANLSYVLNPGDGAFYGPKLEFTLKDAIGREWQCG
TLQMDFVLPERLGAYYIGSDGKKHHPVMLHRAILGTFERFIGILIEHHSGKFPMWLAPIQLSILTISEDSINYANSLKIK
AEEHNIRVELDTTNEKINYKIRNHIHKKVPVFWIVGKKEVEENSVSIRYLESNKQHVMPIDKALKTLLTCASI
>Mature_633_residues
MINIHFSNNLCKQFHRGIKGHDIVNDLFPKLKNETIAAKVNGELYDLSREIIENCTFEVITINSEEGLEIIRHDTAHIMA
QAVKEMFPDVQITIGPTIKDGFYYDFATNHNFSSDDLEIIEKKMIEIINKNESFIREVWSREEAIKFFSSIGEDYKVKII
SNIPSNENITVYKQGSFTDLCRGPHAPSTKTSRAFKLTKVSGSYWQGNSNNERLQRIYGTAWRNEEELKLYLNNLIEAEK
RDHRKIGRELELFHIQNEACGQIFWHTKGWTIYRIIENYIRKKLENNGYIEVKTPILLNKELWEKSGHWDKFRENMFLSE
AEDKTLAIKPMNCPCHIQIFNSKIRSYRDLPIRMAEFGTCHRYEASGALHGLMRVRGFTQDDAHIFCTESQITSEALKFC
NLLIEIYKDFGFTDILVKFSDRPKNRAGSDEIWDKAEAALKKSVEVANLSYVLNPGDGAFYGPKLEFTLKDAIGREWQCG
TLQMDFVLPERLGAYYIGSDGKKHHPVMLHRAILGTFERFIGILIEHHSGKFPMWLAPIQLSILTISEDSINYANSLKIK
AEEHNIRVELDTTNEKINYKIRNHIHKKVPVFWIVGKKEVEENSVSIRYLESNKQHVMPIDKALKTLLTCASI

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=599, Percent_Identity=39.2320534223706, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI38202255, Length=589, Percent_Identity=39.7283531409168, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI20070344, Length=628, Percent_Identity=34.7133757961783, Blast_Score=396, Evalue=1e-110,
Organism=Escherichia coli, GI1788013, Length=628, Percent_Identity=47.7707006369427, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=169, Percent_Identity=26.6272189349112, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71984192, Length=597, Percent_Identity=38.6934673366834, Blast_Score=451, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI71984184, Length=597, Percent_Identity=38.6934673366834, Blast_Score=451, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6322112, Length=629, Percent_Identity=38.3147853736089, Blast_Score=438, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6322654, Length=399, Percent_Identity=36.5914786967419, Blast_Score=263, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24583839, Length=598, Percent_Identity=40.3010033444816, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24583842, Length=598, Percent_Identity=40.3010033444816, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI28574141, Length=598, Percent_Identity=40.3010033444816, Blast_Score=462, Evalue=1e-130,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73285; Mature: 73285

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINIHFSNNLCKQFHRGIKGHDIVNDLFPKLKNETIAAKVNGELYDLSREIIENCTFEVI
CEEEEECCHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCEEEE
TINSEEGLEIIRHDTAHIMAQAVKEMFPDVQITIGPTIKDGFYYDFATNHNFSSDDLEII
EECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCEECCCEEEEEECCCCCCCHHHHHH
EKKMIEIINKNESFIREVWSREEAIKFFSSIGEDYKVKIISNIPSNENITVYKQGSFTDL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCHHH
CRGPHAPSTKTSRAFKLTKVSGSYWQGNSNNERLQRIYGTAWRNEEELKLYLNNLIEAEK
HCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RDHRKIGRELELFHIQNEACGQIFWHTKGWTIYRIIENYIRKKLENNGYIEVKTPILLNK
HHHHHHCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCEEECH
ELWEKSGHWDKFRENMFLSEAEDKTLAIKPMNCPCHIQIFNSKIRSYRDLPIRMAEFGTC
HHHHCCCCHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEECHHHHHHCCCCEEECCCCCC
HRYEASGALHGLMRVRGFTQDDAHIFCTESQITSEALKFCNLLIEIYKDFGFTDILVKFS
EEECCCHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
DRPKNRAGSDEIWDKAEAALKKSVEVANLSYVLNPGDGAFYGPKLEFTLKDAIGREWQCG
CCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCCEECCEEEEEEHHHCCCCCCCC
TLQMDFVLPERLGAYYIGSDGKKHHPVMLHRAILGTFERFIGILIEHHSGKFPMWLAPIQ
EEEEEEECHHHHCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEECEE
LSILTISEDSINYANSLKIKAEEHNIRVELDTTNEKINYKIRNHIHKKVPVFWIVGKKEV
EEEEEEECCCCCCCCEEEEEEECCEEEEEEECCCCEEEEHHHHHHHHCCCEEEEECCCCC
EENSVSIRYLESNKQHVMPIDKALKTLLTCASI
CCCCEEEEEEECCCCEECCHHHHHHHHHHHHCC
>Mature Secondary Structure
MINIHFSNNLCKQFHRGIKGHDIVNDLFPKLKNETIAAKVNGELYDLSREIIENCTFEVI
CEEEEECCHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCEEEE
TINSEEGLEIIRHDTAHIMAQAVKEMFPDVQITIGPTIKDGFYYDFATNHNFSSDDLEII
EECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCEECCCEEEEEECCCCCCCHHHHHH
EKKMIEIINKNESFIREVWSREEAIKFFSSIGEDYKVKIISNIPSNENITVYKQGSFTDL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCHHH
CRGPHAPSTKTSRAFKLTKVSGSYWQGNSNNERLQRIYGTAWRNEEELKLYLNNLIEAEK
HCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RDHRKIGRELELFHIQNEACGQIFWHTKGWTIYRIIENYIRKKLENNGYIEVKTPILLNK
HHHHHHCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCEEECH
ELWEKSGHWDKFRENMFLSEAEDKTLAIKPMNCPCHIQIFNSKIRSYRDLPIRMAEFGTC
HHHHCCCCHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEECHHHHHHCCCCEEECCCCCC
HRYEASGALHGLMRVRGFTQDDAHIFCTESQITSEALKFCNLLIEIYKDFGFTDILVKFS
EEECCCHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
DRPKNRAGSDEIWDKAEAALKKSVEVANLSYVLNPGDGAFYGPKLEFTLKDAIGREWQCG
CCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCCEECCEEEEEEHHHCCCCCCCC
TLQMDFVLPERLGAYYIGSDGKKHHPVMLHRAILGTFERFIGILIEHHSGKFPMWLAPIQ
EEEEEEECHHHHCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEECEE
LSILTISEDSINYANSLKIKAEEHNIRVELDTTNEKINYKIRNHIHKKVPVFWIVGKKEV
EEEEEEECCCCCCCCEEEEEEECCEEEEEEECCCCEEEEHHHHHHHHCCCEEEEECCCCC
EENSVSIRYLESNKQHVMPIDKALKTLLTCASI
CCCCEEEEEEECCCCEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA