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The map label for this gene is nuoN [H]

Identifier: 88657624

GI number: 88657624

Start: 557373

End: 558782

Strand: Direct

Name: nuoN [H]

Synonym: ECH_0556

Alternate gene names: 88657624

Gene position: 557373-558782 (Clockwise)

Preceding gene: 88658135

Following gene: 88658276

Centisome position: 47.39

GC content: 26.88

Gene sequence:

>1410_bases
ATGTATTGGAATAATTTTGTTTATATAATACCAGAAATTTTTTTAGTTAGTTCATCTTTAATCTTCTTATTATTGGGGAT
TGTGCTGAATAAAAAAGTTATACATGTTTTTTCATTGGTTAGTTTATTAGTTACTATTGGTATTGTCATAATTAATTTAG
ATGTTGAACCAAAGCTAATTTTTGATGGACTTTTAAAATCTAGTTTGTATATTTCTATTGCAAAAATTATCATTTTATTT
TCTAGCAGTAGTGTTCTATTCATGATGTTAGCATCTGGTAGGGAATATTGTTATGAATTTTCAATAATGATTTTGCTTGC
GGTTTTTGGTTTAATTACTTTGATTTCTGCAAACAATTTACTTTCTTTTTATTTGTCATTTGAAATTCAAAGCGTGACTT
TGTATGCATTAACATGTTTTGATAAAAATGCAATTAGATCTTCTGAATCAGGTATAAAATATTTTGTTTTAAGTGCGCTC
TCTTCTTGTATAATGTTATATGGTATTTCTATGTTGTATGGTTATACAACACAAGTTGATTTTTATGAATTATACAATTT
CTTTTCTTATAGTGAATATATACCTTTAGGTGCTGTTTTAGGTGTAGTACTGGTTTTGATTAGTATGTTCTTTAAATTAT
CAGCAGCCCCATTTCATATGTGGGTTCCTGATGTTTATCAAGGTACTTCAACTGTTATGACAGCATTTTTTTCTATAGTT
CCAAAAAGTACGTTTGTATTTTTATTAATTAGAATATTAAATGAAGTATTGCCTAGTTTATCTAAAGACTGGCAACATAT
AGTGATTTGTGTATCAATATTATCTATATTTGTTTCAGCATTTGGAGCAATGAGACAAAATAATTTAAAAAGGTTATTTT
CATATGCTGCTGTTGGGCATATGGGGTATATGTTAATTTCATTAGCAATGAATACTGTTGCTAGTAATATAGCTACAATA
ATGTATCTATTGTTATATATTGTTATGAATATAGGACTTTTTTCTATTTTAATACAATATCGTGATGATGATTGTAACCT
ATTAAATTTGAAAGGCTTACATAATAGGTCTCCGGTAATAGCATTTTGTATAGCTGTGATTATGCTATCTATGGCTGGAA
TACCACCTCTAGCTGGATTTTATGCTAAGTATGATGTGTTGTTAAGTTTGGTAGAAAATGGTTTTATAAAGGTTGCAATA
GCTTTTGTTATTGTAAGTGTTATTTCATGCTATTATTATCTACGAATTATTAAGGTAATGTATTTTGATTTTAGTGATAC
TTCCAATAATATATCATTAGCAGACAGGAATTTGTCTTTCATTCTGTTGTTTACAGTGTTAATAAATTGTATATTTTTTG
TATTTGTTGGTGATGTTAGGCGTTTAATAGGTTATTTTTTTACATTTTAA

Upstream 100 bases:

>100_bases
ATTATATCCTTATTATATCTTATTAAAATGCTTAACTCCATTCATAGAGCAGTTGTCTTTAAGAAACTTTGTACTTTAGT
AGCATTTGAGATTATTTATT

Downstream 100 bases:

>100_bases
TTTTTTTAATGGATAATAATCTGAATATTCGATATATAATGTAATAAATTTATTGAATATTAAAGCATGTGAAAACAGTT
TCAATATTTGGCTCGACTGG

Product: NADH dehydrogenase I, N subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MYWNNFVYIIPEIFLVSSSLIFLLLGIVLNKKVIHVFSLVSLLVTIGIVIINLDVEPKLIFDGLLKSSLYISIAKIIILF
SSSSVLFMMLASGREYCYEFSIMILLAVFGLITLISANNLLSFYLSFEIQSVTLYALTCFDKNAIRSSESGIKYFVLSAL
SSCIMLYGISMLYGYTTQVDFYELYNFFSYSEYIPLGAVLGVVLVLISMFFKLSAAPFHMWVPDVYQGTSTVMTAFFSIV
PKSTFVFLLIRILNEVLPSLSKDWQHIVICVSILSIFVSAFGAMRQNNLKRLFSYAAVGHMGYMLISLAMNTVASNIATI
MYLLLYIVMNIGLFSILIQYRDDDCNLLNLKGLHNRSPVIAFCIAVIMLSMAGIPPLAGFYAKYDVLLSLVENGFIKVAI
AFVIVSVISCYYYLRIIKVMYFDFSDTSNNISLADRNLSFILLFTVLINCIFFVFVGDVRRLIGYFFTF

Sequences:

>Translated_469_residues
MYWNNFVYIIPEIFLVSSSLIFLLLGIVLNKKVIHVFSLVSLLVTIGIVIINLDVEPKLIFDGLLKSSLYISIAKIIILF
SSSSVLFMMLASGREYCYEFSIMILLAVFGLITLISANNLLSFYLSFEIQSVTLYALTCFDKNAIRSSESGIKYFVLSAL
SSCIMLYGISMLYGYTTQVDFYELYNFFSYSEYIPLGAVLGVVLVLISMFFKLSAAPFHMWVPDVYQGTSTVMTAFFSIV
PKSTFVFLLIRILNEVLPSLSKDWQHIVICVSILSIFVSAFGAMRQNNLKRLFSYAAVGHMGYMLISLAMNTVASNIATI
MYLLLYIVMNIGLFSILIQYRDDDCNLLNLKGLHNRSPVIAFCIAVIMLSMAGIPPLAGFYAKYDVLLSLVENGFIKVAI
AFVIVSVISCYYYLRIIKVMYFDFSDTSNNISLADRNLSFILLFTVLINCIFFVFVGDVRRLIGYFFTF
>Mature_469_residues
MYWNNFVYIIPEIFLVSSSLIFLLLGIVLNKKVIHVFSLVSLLVTIGIVIINLDVEPKLIFDGLLKSSLYISIAKIIILF
SSSSVLFMMLASGREYCYEFSIMILLAVFGLITLISANNLLSFYLSFEIQSVTLYALTCFDKNAIRSSESGIKYFVLSAL
SSCIMLYGISMLYGYTTQVDFYELYNFFSYSEYIPLGAVLGVVLVLISMFFKLSAAPFHMWVPDVYQGTSTVMTAFFSIV
PKSTFVFLLIRILNEVLPSLSKDWQHIVICVSILSIFVSAFGAMRQNNLKRLFSYAAVGHMGYMLISLAMNTVASNIATI
MYLLLYIVMNIGLFSILIQYRDDDCNLLNLKGLHNRSPVIAFCIAVIMLSMAGIPPLAGFYAKYDVLLSLVENGFIKVAI
AFVIVSVISCYYYLRIIKVMYFDFSDTSNNISLADRNLSFILLFTVLINCIFFVFVGDVRRLIGYFFTF

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831108, Length=303, Percent_Identity=28.3828382838284, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI145693160, Length=389, Percent_Identity=30.5912596401028, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788614, Length=323, Percent_Identity=21.9814241486068, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788613, Length=295, Percent_Identity=22.3728813559322, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788831, Length=282, Percent_Identity=23.758865248227, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 52944; Mature: 52944

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYWNNFVYIIPEIFLVSSSLIFLLLGIVLNKKVIHVFSLVSLLVTIGIVIINLDVEPKLI
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHH
FDGLLKSSLYISIAKIIILFSSSSVLFMMLASGREYCYEFSIMILLAVFGLITLISANNL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHH
LSFYLSFEIQSVTLYALTCFDKNAIRSSESGIKYFVLSALSSCIMLYGISMLYGYTTQVD
HHHHHHEEEEEEEEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
FYELYNFFSYSEYIPLGAVLGVVLVLISMFFKLSAAPFHMWVPDVYQGTSTVMTAFFSIV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHCCHHHHHHHHHHHH
PKSTFVFLLIRILNEVLPSLSKDWQHIVICVSILSIFVSAFGAMRQNNLKRLFSYAAVGH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGYMLISLAMNTVASNIATIMYLLLYIVMNIGLFSILIQYRDDDCNLLNLKGLHNRSPVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHH
AFCIAVIMLSMAGIPPLAGFYAKYDVLLSLVENGFIKVAIAFVIVSVISCYYYLRIIKVM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YFDFSDTSNNISLADRNLSFILLFTVLINCIFFVFVGDVRRLIGYFFTF
HHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYWNNFVYIIPEIFLVSSSLIFLLLGIVLNKKVIHVFSLVSLLVTIGIVIINLDVEPKLI
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHH
FDGLLKSSLYISIAKIIILFSSSSVLFMMLASGREYCYEFSIMILLAVFGLITLISANNL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHH
LSFYLSFEIQSVTLYALTCFDKNAIRSSESGIKYFVLSALSSCIMLYGISMLYGYTTQVD
HHHHHHEEEEEEEEEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
FYELYNFFSYSEYIPLGAVLGVVLVLISMFFKLSAAPFHMWVPDVYQGTSTVMTAFFSIV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHCCHHHHHHHHHHHH
PKSTFVFLLIRILNEVLPSLSKDWQHIVICVSILSIFVSAFGAMRQNNLKRLFSYAAVGH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGYMLISLAMNTVASNIATIMYLLLYIVMNIGLFSILIQYRDDDCNLLNLKGLHNRSPVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHH
AFCIAVIMLSMAGIPPLAGFYAKYDVLLSLVENGFIKVAIAFVIVSVISCYYYLRIIKVM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YFDFSDTSNNISLADRNLSFILLFTVLINCIFFVFVGDVRRLIGYFFTF
HHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA