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The map label for this gene is purH [H]

Identifier: 88657596

GI number: 88657596

Start: 1103262

End: 1104776

Strand: Direct

Name: purH [H]

Synonym: ECH_1074

Alternate gene names: 88657596

Gene position: 1103262-1104776 (Clockwise)

Preceding gene: 88658660

Following gene: 88657982

Centisome position: 93.8

GC content: 31.42

Gene sequence:

>1515_bases
ATGAAAATAAAAAGAGCAATTATATCTGTATATAATAAAACAAACATTATAGAACTTGCAAAATTTTTAATTGAACAAAA
GGTAGAAATTATTGCAACAAGTAGTACTTATAAAGCTTTATTAGATGCAGGATTACAAGTAACAGAAGTATCTAGTTACA
CAAAGTTCCCAGAAATAATGGATGGGAGAGTAAAAACTTTACATCCAAAAATTCATGGTGGAATATTAAGTAATCGCAAC
ACTCATGCTGCAGAATGTCTAAAGCTAGACATACATGATATAGATTTAGTGATTGTGAATTTATATCCCTTCTCTCAAGT
TGCAAATAATAAAACTTCAACCGAAAATGAGATAATAGAACAAATAGATATAGGGGGAGTTACTTTATTAAGAGCTGGAG
CAAAAAACTTCCACAATGTAACAGTAATATCAGATATCAATGACTATGATACATTAAAGGCAGAAATGATTAATAATCAA
AATTCTACAAACTTAACATATAGAAAACAATTAGCAAAAAAAGCATTTGCTATAACTTCATCTTATGATAGTAGCATATA
TAATTGGTTAAATAAAGATAGTAAAGATATACTACCTGAAACGCTTGTCATACATGGAAATAAGGTACAGGATCTCAGAT
GCGGGGAAAATCCACATCAAAAAGGTGCGTTTTACAGTACTTTAGGGGATAAATACCCTCTCAAACAATTACATGGAAAA
GAATTAAGCTACAACAATATAGTAGATATAGAATCTGCTATAAACATAGTGACAGAATTTACGCAACCAGCAGCAGTGAT
TATAAAACATAGTAATCCTTGTGGTGCAGCAACTGCTGATGACATTACAACTGCATACAACAAAGCTTTTGCTTGTGATC
CAAAAAGCAGTTTTGGTGGTATTGTTGCATTAAATAGAGAAATCAACGAAGATATAGCAGAGGAAATCAATAAAATTTTC
ATCGAAGTAATAGTTGGTCCATCTATTACAGACAAAGCAATGGAAATTATACAAAAGAAGAAAAATGTGCGTATTATGCT
GTCCACAGAGTATAATCCACCAAAATATATAATTAAAAATGTCAGTAACGGATTTCTACTACAAGAAAGTAATACAAACC
AATTATCAGAGAAAGACTTAATACAAGTTACAAATTTCCCAGTATCAGATGATACTATTTCTAATCTATTATTTGCTTGG
AAAATATGTAAACATGTAAAATCCAATGCAATTGTTATTGCAAAAGATCACCGTGCAATTGGAGTTGGGGCTGGTCAAAT
GAGCCGTGTTGATAGTCTTGAAATTGCAATAAAAAAAGCCCAAGATTGCGCAGGAGCAGTTTTAGCGTCCGACGCATTTT
TTCCCTTTACAGACAGTATATTACTAAGTGCATCTGTAAATATAAGTGCAATAATTCAACCTGGAGGATCACTAAGAGAT
CAAGAAGTCATAGAAGAAGCAAACAATAAAAAGATTGCTATGTTTTTCACCAACATTAGAAACTTTTATCACTAA

Upstream 100 bases:

>100_bases
ATGTAGAGGGTACATTAGTTGCAGAAGCACAAATTCTAGCAATGACTAAACAAAATGAAGCATGATTTTTTTAAATTTAT
ACATCAATTTTTAGAAAAAT

Downstream 100 bases:

>100_bases
CAGATAACTTATTACTACTCAACATCAATATAACAACCTAAAATAAGCACAATGACTATAGGTGACAATCAAGTAAACAA
ACAAAATGCTATACTTAACA

Product: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MKIKRAIISVYNKTNIIELAKFLIEQKVEIIATSSTYKALLDAGLQVTEVSSYTKFPEIMDGRVKTLHPKIHGGILSNRN
THAAECLKLDIHDIDLVIVNLYPFSQVANNKTSTENEIIEQIDIGGVTLLRAGAKNFHNVTVISDINDYDTLKAEMINNQ
NSTNLTYRKQLAKKAFAITSSYDSSIYNWLNKDSKDILPETLVIHGNKVQDLRCGENPHQKGAFYSTLGDKYPLKQLHGK
ELSYNNIVDIESAINIVTEFTQPAAVIIKHSNPCGAATADDITTAYNKAFACDPKSSFGGIVALNREINEDIAEEINKIF
IEVIVGPSITDKAMEIIQKKKNVRIMLSTEYNPPKYIIKNVSNGFLLQESNTNQLSEKDLIQVTNFPVSDDTISNLLFAW
KICKHVKSNAIVIAKDHRAIGVGAGQMSRVDSLEIAIKKAQDCAGAVLASDAFFPFTDSILLSASVNISAIIQPGGSLRD
QEVIEEANNKKIAMFFTNIRNFYH

Sequences:

>Translated_504_residues
MKIKRAIISVYNKTNIIELAKFLIEQKVEIIATSSTYKALLDAGLQVTEVSSYTKFPEIMDGRVKTLHPKIHGGILSNRN
THAAECLKLDIHDIDLVIVNLYPFSQVANNKTSTENEIIEQIDIGGVTLLRAGAKNFHNVTVISDINDYDTLKAEMINNQ
NSTNLTYRKQLAKKAFAITSSYDSSIYNWLNKDSKDILPETLVIHGNKVQDLRCGENPHQKGAFYSTLGDKYPLKQLHGK
ELSYNNIVDIESAINIVTEFTQPAAVIIKHSNPCGAATADDITTAYNKAFACDPKSSFGGIVALNREINEDIAEEINKIF
IEVIVGPSITDKAMEIIQKKKNVRIMLSTEYNPPKYIIKNVSNGFLLQESNTNQLSEKDLIQVTNFPVSDDTISNLLFAW
KICKHVKSNAIVIAKDHRAIGVGAGQMSRVDSLEIAIKKAQDCAGAVLASDAFFPFTDSILLSASVNISAIIQPGGSLRD
QEVIEEANNKKIAMFFTNIRNFYH
>Mature_504_residues
MKIKRAIISVYNKTNIIELAKFLIEQKVEIIATSSTYKALLDAGLQVTEVSSYTKFPEIMDGRVKTLHPKIHGGILSNRN
THAAECLKLDIHDIDLVIVNLYPFSQVANNKTSTENEIIEQIDIGGVTLLRAGAKNFHNVTVISDINDYDTLKAEMINNQ
NSTNLTYRKQLAKKAFAITSSYDSSIYNWLNKDSKDILPETLVIHGNKVQDLRCGENPHQKGAFYSTLGDKYPLKQLHGK
ELSYNNIVDIESAINIVTEFTQPAAVIIKHSNPCGAATADDITTAYNKAFACDPKSSFGGIVALNREINEDIAEEINKIF
IEVIVGPSITDKAMEIIQKKKNVRIMLSTEYNPPKYIIKNVSNGFLLQESNTNQLSEKDLIQVTNFPVSDDTISNLLFAW
KICKHVKSNAIVIAKDHRAIGVGAGQMSRVDSLEIAIKKAQDCAGAVLASDAFFPFTDSILLSASVNISAIIQPGGSLRD
QEVIEEANNKKIAMFFTNIRNFYH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=467, Percent_Identity=35.117773019272, Blast_Score=243, Evalue=3e-64,
Organism=Escherichia coli, GI1790439, Length=523, Percent_Identity=43.7858508604207, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71985564, Length=471, Percent_Identity=34.3949044585987, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI71985574, Length=303, Percent_Identity=28.0528052805281, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323768, Length=468, Percent_Identity=33.5470085470085, Blast_Score=230, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6323056, Length=468, Percent_Identity=33.7606837606838, Blast_Score=228, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24649832, Length=470, Percent_Identity=33.6170212765957, Blast_Score=224, Evalue=1e-58,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55871; Mature: 55871

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKRAIISVYNKTNIIELAKFLIEQKVEIIATSSTYKALLDAGLQVTEVSSYTKFPEIM
CCHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECHHHHHHHHCCCEEEECHHHHCCHHHH
DGRVKTLHPKIHGGILSNRNTHAAECLKLDIHDIDLVIVNLYPFSQVANNKTSTENEIIE
CCCCEEECCHHCCCCCCCCCCCHHHEEEEECCEEEEEEEEECCHHHHHCCCCCCHHHHHH
QIDIGGVTLLRAGAKNFHNVTVISDINDYDTLKAEMINNQNSTNLTYRKQLAKKAFAITS
HHCCCCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
SYDSSIYNWLNKDSKDILPETLVIHGNKVQDLRCGENPHQKGAFYSTLGDKYPLKQLHGK
CCHHHHHHHHCCCCHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHCCC
ELSYNNIVDIESAINIVTEFTQPAAVIIKHSNPCGAATADDITTAYNKAFACDPKSSFGG
CCCCCCEEEHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCEEECCCCCCCCC
IVALNREINEDIAEEINKIFIEVIVGPSITDKAMEIIQKKKNVRIMLSTEYNPPKYIIKN
EEEECCHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHC
VSNGFLLQESNTNQLSEKDLIQVTNFPVSDDTISNLLFAWKICKHVKSNAIVIAKDHRAI
CCCCEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCEE
GVGAGQMSRVDSLEIAIKKAQDCAGAVLASDAFFPFTDSILLSASVNISAIIQPGGSLRD
EECCCCHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEECCCCCCCH
QEVIEEANNKKIAMFFTNIRNFYH
HHHHHHCCCCEEEEEEEHHHHHCC
>Mature Secondary Structure
MKIKRAIISVYNKTNIIELAKFLIEQKVEIIATSSTYKALLDAGLQVTEVSSYTKFPEIM
CCHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECHHHHHHHHCCCEEEECHHHHCCHHHH
DGRVKTLHPKIHGGILSNRNTHAAECLKLDIHDIDLVIVNLYPFSQVANNKTSTENEIIE
CCCCEEECCHHCCCCCCCCCCCHHHEEEEECCEEEEEEEEECCHHHHHCCCCCCHHHHHH
QIDIGGVTLLRAGAKNFHNVTVISDINDYDTLKAEMINNQNSTNLTYRKQLAKKAFAITS
HHCCCCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHC
SYDSSIYNWLNKDSKDILPETLVIHGNKVQDLRCGENPHQKGAFYSTLGDKYPLKQLHGK
CCHHHHHHHHCCCCHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHCCC
ELSYNNIVDIESAINIVTEFTQPAAVIIKHSNPCGAATADDITTAYNKAFACDPKSSFGG
CCCCCCEEEHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCEEECCCCCCCCC
IVALNREINEDIAEEINKIFIEVIVGPSITDKAMEIIQKKKNVRIMLSTEYNPPKYIIKN
EEEECCHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHC
VSNGFLLQESNTNQLSEKDLIQVTNFPVSDDTISNLLFAWKICKHVKSNAIVIAKDHRAI
CCCCEEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCEE
GVGAGQMSRVDSLEIAIKKAQDCAGAVLASDAFFPFTDSILLSASVNISAIIQPGGSLRD
EECCCCHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEECCCCCCCH
QEVIEEANNKKIAMFFTNIRNFYH
HHHHHHCCCCEEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA