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The map label for this gene is virB8-2

Identifier: 88608885

GI number: 88608885

Start: 663617

End: 664294

Strand: Reverse

Name: virB8-2

Synonym: NSE_0743

Alternate gene names: 88608885

Gene position: 664294-663617 (Counterclockwise)

Preceding gene: 88608672

Following gene: 88608571

Centisome position: 77.33

GC content: 41.45

Gene sequence:

>678_bases
ATGGTGAGGTTTTTCAAAAGCAAAAGCGAAGCGAAACAAAAGGTGGCCCACTGGTACGAGGAGCACGTAGGACGGGTTAC
TCTGCAAAGGAACTTTCTTGTGGTGCTGAACTTGGGGGTCATAGTAGCCCTTATGTTAGTTGTTTTTGCTTTGTTTGCGG
TTAGTAGTTCAAGAACAATAGAGGCTTTTGTGATCGAAGTTTCCAAGAAAACCGGAACTATAGCACGCGTCAATCCTGCT
ACGGTAAAGGAGTATTCTGCAAACAGGGCAATAACGAATTATTTTGTTACGCAGTATGTAAAGGCAAGGGAGGTTTTTCA
TCCTGCAACGTATCGTTACGATTACTACACCACAGTGAGAGTATTTTCAGATCCTAGCCTTTATACGCTGTTTAAGGGAT
CTCTCTCCTTAAGTAATCCTACAAGTCCGCTCAACTTATACGCTAATGTAGCGGATTCTAAATACGAGGTTCGCTCCATA
AGACATCTAGACAGTGATACGGTTCAGATGAGAATTACTATGAGATTTGTAAATAGTGATGGTGGAGTGAGTGTCACAAA
TAAGATTGTGTCTGTCAGATACAGTTATATGGATCTTGATCTTTCAGAAGAGGATAGGCGCCTCAATCCTCTTGGTTTTG
TTGTTGTTTCTTATCAGGTAGATGATGATCATTCGTGA

Upstream 100 bases:

>100_bases
TGTTTTTTTGTTGTTCTGCCTACGGAAGTTGATATTTTACTAAGGAACTGTTGTTAGATCTTGTCTAGTATGGTTTGTGT
TCTGTATTTCTGATGGGTGT

Downstream 100 bases:

>100_bases
GTTGTTTTTTGTTGTGTTTTTCGCACTTTTCTGCATACGAGCATATGCCCTCGAGCATGATCCGCGTGTGCGAACTCTCG
TCTATTCGGAGAGCACAATC

Product: type IV secretion system protein VirB8

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MVRFFKSKSEAKQKVAHWYEEHVGRVTLQRNFLVVLNLGVIVALMLVVFALFAVSSSRTIEAFVIEVSKKTGTIARVNPA
TVKEYSANRAITNYFVTQYVKAREVFHPATYRYDYYTTVRVFSDPSLYTLFKGSLSLSNPTSPLNLYANVADSKYEVRSI
RHLDSDTVQMRITMRFVNSDGGVSVTNKIVSVRYSYMDLDLSEEDRRLNPLGFVVVSYQVDDDHS

Sequences:

>Translated_225_residues
MVRFFKSKSEAKQKVAHWYEEHVGRVTLQRNFLVVLNLGVIVALMLVVFALFAVSSSRTIEAFVIEVSKKTGTIARVNPA
TVKEYSANRAITNYFVTQYVKAREVFHPATYRYDYYTTVRVFSDPSLYTLFKGSLSLSNPTSPLNLYANVADSKYEVRSI
RHLDSDTVQMRITMRFVNSDGGVSVTNKIVSVRYSYMDLDLSEEDRRLNPLGFVVVSYQVDDDHS
>Mature_225_residues
MVRFFKSKSEAKQKVAHWYEEHVGRVTLQRNFLVVLNLGVIVALMLVVFALFAVSSSRTIEAFVIEVSKKTGTIARVNPA
TVKEYSANRAITNYFVTQYVKAREVFHPATYRYDYYTTVRVFSDPSLYTLFKGSLSLSNPTSPLNLYANVADSKYEVRSI
RHLDSDTVQMRITMRFVNSDGGVSVTNKIVSVRYSYMDLDLSEEDRRLNPLGFVVVSYQVDDDHS

Specific function: Component of the type IV secretion system virB/virD4 which could be a major virulence determinant for subversion of human endothelial cell (HEC) function [H]

COG id: COG3736

COG function: function code U; Type IV secretory pathway, component VirB8

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the virB8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007430 [H]

Pfam domain/function: PF04335 VirB8 [H]

EC number: NA

Molecular weight: Translated: 25685; Mature: 25685

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRFFKSKSEAKQKVAHWYEEHVGRVTLQRNFLVVLNLGVIVALMLVVFALFAVSSSRTI
CCEEECCHHHHHHHHHHHHHHHHCEEEEECCEEEEEHHHHHHHHHHHHHHHHHCCCCCEE
EAFVIEVSKKTGTIARVNPATVKEYSANRAITNYFVTQYVKAREVFHPATYRYDYYTTVR
EEEEEEEECCCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
VFSDPSLYTLFKGSLSLSNPTSPLNLYANVADSKYEVRSIRHLDSDTVQMRITMRFVNSD
EECCCCEEEEEECCEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEEEEEECCC
GGVSVTNKIVSVRYSYMDLDLSEEDRRLNPLGFVVVSYQVDDDHS
CCEEEEEHEEEEEEEEEECCCCCCCCCCCCEEEEEEEEEECCCCC
>Mature Secondary Structure
MVRFFKSKSEAKQKVAHWYEEHVGRVTLQRNFLVVLNLGVIVALMLVVFALFAVSSSRTI
CCEEECCHHHHHHHHHHHHHHHHCEEEEECCEEEEEHHHHHHHHHHHHHHHHHCCCCCEE
EAFVIEVSKKTGTIARVNPATVKEYSANRAITNYFVTQYVKAREVFHPATYRYDYYTTVR
EEEEEEEECCCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEE
VFSDPSLYTLFKGSLSLSNPTSPLNLYANVADSKYEVRSIRHLDSDTVQMRITMRFVNSD
EECCCCEEEEEECCEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEEEEEECCC
GGVSVTNKIVSVRYSYMDLDLSEEDRRLNPLGFVVVSYQVDDDHS
CCEEEEEHEEEEEEEEEECCCCCCCCCCCCEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA