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The map label for this gene is nusA

Identifier: 88608874

GI number: 88608874

Start: 444417

End: 446030

Strand: Direct

Name: nusA

Synonym: NSE_0521

Alternate gene names: 88608874

Gene position: 444417-446030 (Clockwise)

Preceding gene: 88608070

Following gene: 88608686

Centisome position: 51.74

GC content: 42.32

Gene sequence:

>1614_bases
ATGTCTTTTTCCAATAATGGACTCAGTAATCTCCAAATTGTGGAATCCATAAATTCTGTCGCAGAAAAAGAGGGACTTAG
TCCTGATACTCTGTTTCGCGCGATAGGAATCGAGTTAGCACATGAAATAGGAAAAAGGCAGTATGGAGACCATAGAATTT
TTGTTGAGATAGACAAAAAAAGTGGTGAGATACTTGTATCAAAGCGACTTCTTGTAGTCGAAGATTCTGATAAAGCTCGT
ATGCTTGAGCAGATGGAAGTTACTACCGAAGAGTCCGACGATTTGCCTTCCTCATTTCGTGCGGAGGAAAAGGTTCATTA
TGATGGTGTAATCGACTTGTCAACTGCAAGGCTCAAGTACCCGAGTATTGAGCACAAAGCGGGCGATATAATCACAGAAC
ATTTACCTTCATTTTCTTCTGGATACATAATTGCCAGAGTCATGAAAGCGAAGCTTGAGCGCCTCATTACCTCATTAGTG
AGGGAAAAACAGTATCACTGTTACAAAGGCAGAGTTGGTGAAATTGTTACAGGCATCGTGAAAAAATCCATTGATTTTAA
GACGGGTAGCCGAAGCATTATCGTCGATATCGCAGGAGTGGAGGGCCTTTTACCTTATTCATCACTAGTAAAAGGGGAGA
GCTTTAGGCCTGGGGAAAGAGTCAAGTGTGTTATACAAAAAGTTGAGTATTCAGTTGTCAAACCTCAAATCTTGCTCTCT
AGGTCTAGTGGCAGTTTTGTCGCTCAGCTCTTTTCTCAGCAGGTGCCAGAAATATATGATCGTGTAGTAGAAATAAGGAA
AGTCGCCAGAGATGCTGGCTCCAGAAGCAAGGTCGCTGTTTTTTCATCGGATAGAAACATAGATCCAGTTGGAGCATGTA
TTGGCATGGGTGGAAGTAGAATAAACGCCGTAGTAAACGAGTTGCACGGTGAAAAGATAGATATAGTCGAATATTCGAAC
GATACTGCCACCTTCCTAGCCAATGCCCTTAAACCAATCAGACCAGTTAAGATTACTGTAAACGAAGAGACAAAAAAAAT
AGAACTAGTTGTCCCTGATGAAAGTGTCAGCCTTGTTATAGGACGCGGTGGGCAGAATGTATACTTGTTATCCTCTCTCC
TTGGATATCGTGTAGAGGTTCTCAGTGATGCCGAATTTTCCAAAAAGAAAATGGAAGAATTCATTTCGGGGACCGCACGC
TTTGTAGAAGCCCTGAATGTCGAGGAAGTAATAGCTCAGCTATTGGTTACTGAAGGATTCTCAACGGTTGAAGAAATCGC
TGACTGTAATACGTCAAGGCTGGCCTTCATTGAGGGGTTTGATAAAGATATCGCTGAAGAGATAAGGAGTAGGGCGGTTG
AATATGTGAATGAGCAACCTAAGAGAGTACGGGCTCTGGCAGAAAAGTATAAAGCAAATCCGAATATGCTTGCCCTTTCA
AGCTTTGACACGGGATTACTTGAGGTACTTTTCTCATCTGGACTGACGGATCTTGAAAAGGTTGCCGAGTTGTCTTGTGA
TGAATTAAGAGAGGTCATTGGAGATAATGGATTTGGTGTGCCGTTGCTAGAACAGCTTATCATCAGATCAAGGAAGACTC
TGGGTTGGTTATAG

Upstream 100 bases:

>100_bases
TTAAAGTGTTTAAGCATACTATTTCCTACTCAGGGCGTAAGAGCTTCTCCCCTGGTATCAGTCTTTTGTTTGAGCACAGT
AGGCAAGTTTTAGTTCAGTT

Downstream 100 bases:

>100_bases
TGCAGGCATATGGATACTAAAAAAAGGCTCAGCTTGAATCTGAAGAACGGGAAAGAAGAATTGTCGCGCAGGGCCCTGAG
AGAGTTTATCGGCAAGTCTT

Product: N utilization substance protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSFSNNGLSNLQIVESINSVAEKEGLSPDTLFRAIGIELAHEIGKRQYGDHRIFVEIDKKSGEILVSKRLLVVEDSDKAR
MLEQMEVTTEESDDLPSSFRAEEKVHYDGVIDLSTARLKYPSIEHKAGDIITEHLPSFSSGYIIARVMKAKLERLITSLV
REKQYHCYKGRVGEIVTGIVKKSIDFKTGSRSIIVDIAGVEGLLPYSSLVKGESFRPGERVKCVIQKVEYSVVKPQILLS
RSSGSFVAQLFSQQVPEIYDRVVEIRKVARDAGSRSKVAVFSSDRNIDPVGACIGMGGSRINAVVNELHGEKIDIVEYSN
DTATFLANALKPIRPVKITVNEETKKIELVVPDESVSLVIGRGGQNVYLLSSLLGYRVEVLSDAEFSKKKMEEFISGTAR
FVEALNVEEVIAQLLVTEGFSTVEEIADCNTSRLAFIEGFDKDIAEEIRSRAVEYVNEQPKRVRALAEKYKANPNMLALS
SFDTGLLEVLFSSGLTDLEKVAELSCDELREVIGDNGFGVPLLEQLIIRSRKTLGWL

Sequences:

>Translated_537_residues
MSFSNNGLSNLQIVESINSVAEKEGLSPDTLFRAIGIELAHEIGKRQYGDHRIFVEIDKKSGEILVSKRLLVVEDSDKAR
MLEQMEVTTEESDDLPSSFRAEEKVHYDGVIDLSTARLKYPSIEHKAGDIITEHLPSFSSGYIIARVMKAKLERLITSLV
REKQYHCYKGRVGEIVTGIVKKSIDFKTGSRSIIVDIAGVEGLLPYSSLVKGESFRPGERVKCVIQKVEYSVVKPQILLS
RSSGSFVAQLFSQQVPEIYDRVVEIRKVARDAGSRSKVAVFSSDRNIDPVGACIGMGGSRINAVVNELHGEKIDIVEYSN
DTATFLANALKPIRPVKITVNEETKKIELVVPDESVSLVIGRGGQNVYLLSSLLGYRVEVLSDAEFSKKKMEEFISGTAR
FVEALNVEEVIAQLLVTEGFSTVEEIADCNTSRLAFIEGFDKDIAEEIRSRAVEYVNEQPKRVRALAEKYKANPNMLALS
SFDTGLLEVLFSSGLTDLEKVAELSCDELREVIGDNGFGVPLLEQLIIRSRKTLGWL
>Mature_536_residues
SFSNNGLSNLQIVESINSVAEKEGLSPDTLFRAIGIELAHEIGKRQYGDHRIFVEIDKKSGEILVSKRLLVVEDSDKARM
LEQMEVTTEESDDLPSSFRAEEKVHYDGVIDLSTARLKYPSIEHKAGDIITEHLPSFSSGYIIARVMKAKLERLITSLVR
EKQYHCYKGRVGEIVTGIVKKSIDFKTGSRSIIVDIAGVEGLLPYSSLVKGESFRPGERVKCVIQKVEYSVVKPQILLSR
SSGSFVAQLFSQQVPEIYDRVVEIRKVARDAGSRSKVAVFSSDRNIDPVGACIGMGGSRINAVVNELHGEKIDIVEYSND
TATFLANALKPIRPVKITVNEETKKIELVVPDESVSLVIGRGGQNVYLLSSLLGYRVEVLSDAEFSKKKMEEFISGTARF
VEALNVEEVIAQLLVTEGFSTVEEIADCNTSRLAFIEGFDKDIAEEIRSRAVEYVNEQPKRVRALAEKYKANPNMLALSS
FDTGLLEVLFSSGLTDLEKVAELSCDELREVIGDNGFGVPLLEQLIIRSRKTLGWL

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=515, Percent_Identity=31.4563106796116, Blast_Score=226, Evalue=2e-60,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 59562; Mature: 59431

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSNNGLSNLQIVESINSVAEKEGLSPDTLFRAIGIELAHEIGKRQYGDHRIFVEIDKK
CCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
SGEILVSKRLLVVEDSDKARMLEQMEVTTEESDDLPSSFRAEEKVHYDGVIDLSTARLKY
CCCEEEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCEEEECCCCEEC
PSIEHKAGDIITEHLPSFSSGYIIARVMKAKLERLITSLVREKQYHCYKGRVGEIVTGIV
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KKSIDFKTGSRSIIVDIAGVEGLLPYSSLVKGESFRPGERVKCVIQKVEYSVVKPQILLS
HHCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEE
RSSGSFVAQLFSQQVPEIYDRVVEIRKVARDAGSRSKVAVFSSDRNIDPVGACIGMGGSR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCHH
INAVVNELHGEKIDIVEYSNDTATFLANALKPIRPVKITVNEETKKIELVVPDESVSLVI
HHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCEEEEE
GRGGQNVYLLSSLLGYRVEVLSDAEFSKKKMEEFISGTARFVEALNVEEVIAQLLVTEGF
ECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCH
STVEEIADCNTSRLAFIEGFDKDIAEEIRSRAVEYVNEQPKRVRALAEKYKANPNMLALS
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEE
SFDTGLLEVLFSSGLTDLEKVAELSCDELREVIGDNGFGVPLLEQLIIRSRKTLGWL
CCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFSNNGLSNLQIVESINSVAEKEGLSPDTLFRAIGIELAHEIGKRQYGDHRIFVEIDKK
CCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
SGEILVSKRLLVVEDSDKARMLEQMEVTTEESDDLPSSFRAEEKVHYDGVIDLSTARLKY
CCCEEEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCEEEECCCCEEC
PSIEHKAGDIITEHLPSFSSGYIIARVMKAKLERLITSLVREKQYHCYKGRVGEIVTGIV
CCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KKSIDFKTGSRSIIVDIAGVEGLLPYSSLVKGESFRPGERVKCVIQKVEYSVVKPQILLS
HHCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEE
RSSGSFVAQLFSQQVPEIYDRVVEIRKVARDAGSRSKVAVFSSDRNIDPVGACIGMGGSR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCCHH
INAVVNELHGEKIDIVEYSNDTATFLANALKPIRPVKITVNEETKKIELVVPDESVSLVI
HHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCEEEEE
GRGGQNVYLLSSLLGYRVEVLSDAEFSKKKMEEFISGTARFVEALNVEEVIAQLLVTEGF
ECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCH
STVEEIADCNTSRLAFIEGFDKDIAEEIRSRAVEYVNEQPKRVRALAEKYKANPNMLALS
HHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEE
SFDTGLLEVLFSSGLTDLEKVAELSCDELREVIGDNGFGVPLLEQLIIRSRKTLGWL
CCCHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]